FastQCFastQC Report
Thu 26 May 2016
SRR938656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938656_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405651
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123900.5150373017532468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105720.43946524246451374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74660.3103525823155562No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26240.10907650361586116No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64300.061.5544621
GTATCAA115150.043.119911
TGGTATC23550.032.4765742
TATCAAC157200.031.4581682
ATCAACG158350.031.0758323
TCAACGC160700.030.6503124
CAACGCA164300.029.9787315
AACGCAG170000.028.973566
GTGGTAT30100.026.9932461
ACGCAGA194400.025.239237
CGCAGAG196650.024.9746048
GCAGAGT208600.023.065739
GAGTACT114600.022.77482812-13
TACATGG152950.022.672982
GTACATG156400.022.4204921
GTACTTT118150.021.8694214-15
ACATGGG153250.021.4789893
CAGAGTA202650.021.21162810-11
AGAGTAC200150.020.97822410-11
AGTACTT118050.020.84182712-13
Produced by FastQC (version 0.11.5)