##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938656_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2405651 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.795690646731384 31.0 31.0 34.0 30.0 34.0 2 32.042610503352314 31.0 31.0 34.0 30.0 34.0 3 32.08829252455988 33.0 31.0 34.0 30.0 34.0 4 35.75370575366086 37.0 35.0 37.0 35.0 37.0 5 35.650455947267496 37.0 35.0 37.0 33.0 37.0 6 35.69113849016337 37.0 35.0 37.0 33.0 37.0 7 35.61508797410763 37.0 35.0 37.0 33.0 37.0 8 35.59554316066628 37.0 35.0 37.0 33.0 37.0 9 37.12225297850769 39.0 37.0 39.0 33.0 39.0 10-11 37.0499762850056 39.0 37.0 39.0 33.0 39.0 12-13 36.929619051142495 39.0 37.0 39.0 33.0 39.0 14-15 37.89906515949321 40.0 37.0 41.0 32.5 41.0 16-17 37.99835408378023 40.0 37.0 41.0 33.0 41.0 18-19 38.17172420272101 40.0 37.5 41.0 33.0 41.0 20-21 38.14052932033782 40.0 37.0 41.0 33.0 41.0 22-23 38.00450127636968 40.0 37.0 41.0 33.0 41.0 24-25 37.88265484062318 40.0 37.0 41.0 32.5 41.0 26-27 37.65842613911993 40.0 37.0 41.0 32.0 41.0 28-29 37.506346099247146 39.5 36.5 41.0 32.0 41.0 30-31 37.221026449805066 39.0 36.0 41.0 31.0 41.0 32-33 37.07830001109886 39.0 36.0 41.0 31.0 41.0 34-35 36.916595133708086 39.0 35.0 41.0 31.0 41.0 36-37 36.771071739001215 39.0 35.0 41.0 30.0 41.0 38-39 36.54545318502143 39.0 35.0 40.0 30.0 41.0 40-41 36.30206147940828 39.0 35.0 40.0 30.0 41.0 42-43 35.99971130475701 38.0 35.0 40.0 29.5 41.0 44-45 35.63894617299018 38.0 35.0 40.0 28.5 41.0 46-47 35.13890834539175 38.0 34.0 40.0 26.5 41.0 48-49 34.65728258172112 38.0 33.5 40.0 24.5 41.0 50-51 34.2438423112912 38.0 33.0 40.0 24.0 41.0 52-53 33.64588379611174 37.0 33.0 40.0 22.5 41.0 54-55 33.218810209793524 37.0 32.0 40.0 20.5 41.0 56-57 32.82239984104095 36.5 31.0 39.0 19.0 40.5 58-59 32.28835708088995 36.0 31.0 39.0 16.5 40.0 60-61 31.805746760440314 35.0 30.0 39.0 13.5 40.0 62-63 31.303977592759715 35.0 29.5 38.5 8.5 40.0 64-65 30.654445719682528 34.5 29.0 38.0 7.5 40.0 66-67 30.00151933925578 34.0 28.0 37.5 4.0 40.0 68-69 29.265624149138837 34.0 27.0 37.0 2.0 39.0 70-71 28.508592060943172 33.0 26.0 36.0 2.0 39.0 72-73 27.937859232282655 33.0 26.0 36.0 2.0 38.5 74-75 27.23722746982002 32.0 24.5 35.0 2.0 38.0 76-77 26.08690080148783 30.5 23.0 34.0 2.0 36.0 78-79 26.58024563829084 32.0 24.0 35.0 2.0 36.0 80-81 26.200719888296348 32.0 23.0 35.0 2.0 36.0 82-83 25.533666978294026 31.0 20.5 34.5 2.0 36.0 84-85 24.758660129835956 31.0 18.5 34.0 2.0 35.0 86-87 23.856712798323613 30.5 10.0 34.0 2.0 35.0 88-89 22.890448988652135 29.5 4.5 34.0 2.0 35.0 90-91 22.034839633845476 29.0 2.0 34.0 2.0 35.0 92-93 20.958567971829662 28.5 2.0 33.5 2.0 35.0 94-95 19.795095797353817 26.5 2.0 33.0 2.0 34.5 96-97 18.418110731772813 25.0 2.0 32.5 2.0 34.0 98-99 16.672697951614758 11.0 2.0 32.0 2.0 34.0 100-101 12.50917319262021 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2959517898017481 1101 2 0.3135640201582035 1101 3 0.30988197891536373 1101 4 0.1770671646421178 1101 5 0.18594639181274175 1101 6 0.24543710372121552 1101 7 0.23159686217996267 1101 8 0.2577363785266016 1101 9 0.27566327334914575 1101 10-11 0.4071487590723848 1101 12-13 0.30397342327975707 1101 14-15 0.5126058625697851 1101 16-17 0.37801484561355636 1101 18-19 0.4193496545229394 1101 20-21 0.4592051963584325 1101 22-23 0.4667235823862157 1101 24-25 0.5103080367212982 1101 26-27 0.4953233231514247 1101 28-29 0.6078071087075827 1101 30-31 0.5279745179125115 1101 32-33 0.45494161612025863 1101 34-35 0.27461194856508797 1101 36-37 0.2851159533253167 1101 38-39 0.5357222214792969 1101 40-41 0.7747559347425792 1101 42-43 0.6200830936868442 1101 44-45 0.6979457528585513 1101 46-47 0.7650250576210524 1101 48-49 0.7950765081059856 1101 50-51 0.7877536099925422 1101 52-53 0.7709412761537919 1101 54-55 0.7548266515215687 1101 56-57 0.9487585576292901 1101 58-59 0.9872537280148634 1101 60-61 0.9244943506043022 1101 62-63 0.8936328237599298 1101 64-65 1.050512381993748 1101 66-67 1.1686963621036455 1101 68-69 1.3481137522311606 1101 70-71 1.6215112469118829 1101 72-73 1.7703393368054137 1101 74-75 1.5718537546698528 1101 76-77 1.6326037193831269 1101 78-79 1.5745038386672867 1101 80-81 1.9257851994145767 1101 82-83 1.9856657311487744 1101 84-85 2.1645619546929673 1101 86-87 2.3444092787117974 1101 88-89 2.121341663441388 1101 90-91 2.5320312871163857 1101 92-93 2.6464122511990773 1101 94-95 2.8221226946373967 1101 96-97 2.9977865154533987 1101 98-99 2.7310749006350683 1101 100-101 2.2366858951235216 1102 1 0.30875248263212285 1102 2 0.2488064804997876 1102 3 0.2833460409509172 1102 4 0.26106031404369645 1102 5 0.2768218901866746 1102 6 0.26254772450917585 1102 7 0.3069663957330846 1102 8 0.25481303040628234 1102 9 0.35536550334467876 1102 10-11 0.3441926452224422 1102 12-13 0.40357301127463074 1102 14-15 0.5745628785948327 1102 16-17 0.45627911001707133 1102 18-19 0.47273266493022703 1102 20-21 0.29437536153857025 1102 22-23 0.4513161069286582 1102 24-25 0.38621248344816195 1102 26-27 0.4193606601243758 1102 28-29 0.3449752397638193 1102 30-31 0.4253806652780767 1102 32-33 0.3919211656342938 1102 34-35 0.5448736545429043 1102 36-37 0.5607176000925378 1102 38-39 0.5755415859294146 1102 40-41 0.6210050566415006 1102 42-43 0.6757962182979185 1102 44-45 0.6492789520875988 1102 46-47 0.4765816720852314 1102 48-49 0.5052439316681117 1102 50-51 0.5812459592594337 1102 52-53 0.6596439090888424 1102 54-55 0.6178835106947247 1102 56-57 0.6622236515576461 1102 58-59 0.8205457020821925 1102 60-61 1.0015025884092204 1102 62-63 1.1625223629780166 1102 64-65 1.10884597121143 1102 66-67 1.1751201036592036 1102 68-69 1.3329106709240648 1102 70-71 1.444676154034223 1102 72-73 1.652101968457906 1102 74-75 1.4242930844909907 1102 76-77 1.2805389206930897 1102 78-79 1.4291227809186822 1102 80-81 1.695345782279432 1102 82-83 1.865098457482656 1102 84-85 2.025214858750541 1102 86-87 2.002741531064313 1102 88-89 1.7045941059880434 1102 90-91 1.9467895265401616 1102 92-93 2.391109857521112 1102 94-95 2.1007081617850893 1102 96-97 2.232540456020132 1102 98-99 2.1967695827734453 1102 100-101 1.8932189149619738 1103 1 0.1957439901218656 1103 2 0.20740562110324845 1103 3 0.1975318226830538 1103 4 0.12458412678829944 1103 5 0.0977132399598375 1103 6 0.17775010696215787 1103 7 0.21645900744387347 1103 8 0.18337530898562449 1103 9 0.2766664653236077 1103 10-11 0.317358792828351 1103 12-13 0.3638442141120919 1103 14-15 0.4842572732511172 1103 16-17 0.32939125070969766 1103 18-19 0.39548054077786077 1103 20-21 0.4216476229935182 1103 22-23 0.43028733159435006 1103 24-25 0.46882369208599073 1103 26-27 0.47920308701264247 1103 28-29 0.43747166065291054 1103 30-31 0.5171655681424951 1103 32-33 0.39373772454333533 1103 34-35 0.5053405940110949 1103 36-37 0.4203816094550987 1103 38-39 0.5078082058652384 1103 40-41 0.6467381723075007 1103 42-43 0.6360959105507504 1103 44-45 0.7179933723433649 1103 46-47 0.7945203706518456 1103 48-49 0.9080335373356618 1103 50-51 0.997009111787456 1103 52-53 1.1358365959174037 1103 54-55 1.0109236899935823 1103 56-57 0.9970914166040004 1103 58-59 1.2016515833018175 1103 60-61 1.344021093719217 1103 62-63 1.2462768375071462 1103 64-65 1.4781466790401794 1103 66-67 1.096035638691447 1103 68-69 1.011912844522989 1103 70-71 1.4250594493987485 1103 72-73 1.3743980103762894 1103 74-75 1.196876018921266 1103 76-77 1.3835394300182458 1103 78-79 1.3589516087227125 1103 80-81 1.6333945237748821 1103 82-83 1.77776108029202 1103 84-85 2.021840605188597 1103 86-87 2.083163153915695 1103 88-89 2.063888265350453 1103 90-91 2.274871379294975 1103 92-93 2.397045596467887 1103 94-95 2.2949465362077426 1103 96-97 2.2785180682586983 1103 98-99 2.3343553200292995 1103 100-101 2.106355404881537 1104 1 0.1425872738243683 1104 2 0.1660874095595517 1104 3 0.20560468670179688 1104 4 0.1624487767101357 1104 5 0.05508008193371694 1104 6 0.09253903119090978 1104 7 0.06913621532292069 1104 8 0.13625117306416 1104 9 0.16092611742373464 1104 10-11 0.16132967683420674 1104 12-13 0.11675035593913918 1104 14-15 0.23779231903316145 1104 16-17 0.2958533764734028 1104 18-19 0.27107819410998246 1104 20-21 0.2590379847983755 1104 22-23 0.2133108163069366 1104 24-25 0.23337537734167313 1104 26-27 0.3684902690844254 1104 28-29 0.20122938551265435 1104 30-31 0.3516414830952357 1104 32-33 0.3624544510536438 1104 34-35 0.2604017711985662 1104 36-37 0.25649071945065316 1104 38-39 0.31332891195372525 1104 40-41 0.41788955250775217 1104 42-43 0.5011197008882533 1104 44-45 0.4856122219977337 1104 46-47 0.69681094402128 1104 48-49 0.6943357510282269 1104 50-51 0.5770288350355486 1104 52-53 0.609837881060713 1104 54-55 0.6709610483691719 1104 56-57 0.7771817562937642 1104 58-59 0.9020807869970398 1104 60-61 0.8317547140742185 1104 62-63 1.0505831116451105 1104 64-65 1.1772784005342452 1104 66-67 1.2041322313532206 1104 68-69 1.3106460197286438 1104 70-71 1.2581716317789962 1104 72-73 1.5066735208031332 1104 74-75 1.33419956086734 1104 76-77 1.3042805419804253 1104 78-79 1.5594068190106931 1104 80-81 1.6533658785570111 1104 82-83 1.7426674613037925 1104 84-85 2.0678683828872764 1104 86-87 1.9723750687317612 1104 88-89 1.9133495776962768 1104 90-91 2.178258405801767 1104 92-93 2.308241719397447 1104 94-95 2.3076152587240415 1104 96-97 2.156516775522224 1104 98-99 2.3425604949615995 1104 100-101 1.7679672061797795 1105 1 0.12237314858013448 1105 2 0.15079827750802366 1105 3 0.19496579376723133 1105 4 0.07967110798967525 1105 5 0.17535411834197845 1105 6 0.09791661224335968 1105 7 0.1541324968202602 1105 8 0.1027855285811583 1105 9 0.2402651386237764 1105 10-11 0.2104186612713761 1105 12-13 0.1948825348128338 1105 14-15 0.36848379166575285 1105 16-17 0.28666556148245803 1105 18-19 0.31024146768694294 1105 20-21 0.22236244930936522 1105 22-23 0.2523562376234807 1105 24-25 0.2519013129303076 1105 26-27 0.3024751495189051 1105 28-29 0.3699908321333183 1105 30-31 0.40605334402816595 1105 32-33 0.5068333203896032 1105 34-35 0.48236876412250496 1105 36-37 0.42131792341104557 1105 38-39 0.46118978788801 1105 40-41 0.5509275164147027 1105 42-43 0.5207603957311164 1105 44-45 0.6287491571775874 1105 46-47 0.5194466341887463 1105 48-49 0.5912742131512161 1105 50-51 0.7439127368389293 1105 52-53 0.7564599890190138 1105 54-55 0.8272920717982046 1105 56-57 0.8898343573811047 1105 58-59 1.0755942302460753 1105 60-61 0.990544628172227 1105 62-63 1.057815898355031 1105 64-65 1.2719697894389626 1105 66-67 1.2842211095474596 1105 68-69 1.2325351246213785 1105 70-71 1.322704891094073 1105 72-73 1.4631903462361393 1105 74-75 1.5521755837370108 1105 76-77 1.558581445889672 1105 78-79 1.7742069873107553 1105 80-81 2.0033882353067085 1105 82-83 2.227309200986088 1105 84-85 2.334198836403882 1105 86-87 2.4209429350992586 1105 88-89 2.3666535218420464 1105 90-91 2.5205729608725456 1105 92-93 2.3851022372015827 1105 94-95 2.388963309166215 1105 96-97 2.5131488850917556 1105 98-99 2.6147433686007595 1105 100-101 2.566937282963165 1106 1 0.08066316229772497 1106 2 0.10052960976793202 1106 3 0.07301375504104612 1106 4 0.056069754557420026 1106 5 0.059499380123362755 1106 6 -0.04419446372977376 1106 7 0.10796653256867472 1106 8 0.11297847053253207 1106 9 0.11181395182132547 1106 10-11 0.1372154004669639 1106 12-13 0.08651838685025837 1106 14-15 0.06038469429842053 1106 16-17 0.0440268818712255 1106 18-19 0.1157969371200025 1106 20-21 0.12510612257852927 1106 22-23 0.1864488441105081 1106 24-25 0.09870549655449423 1106 26-27 -0.07958861764444691 1106 28-29 0.1412537875404709 1106 30-31 0.2488697976966705 1106 32-33 -0.03867036186385775 1106 34-35 -0.09302407503304977 1106 36-37 -0.10963338224044605 1106 38-39 0.10518238204229391 1106 40-41 0.1206457442755422 1106 42-43 0.07230350417449216 1106 44-45 0.09729265696159217 1106 46-47 -0.36269455709896903 1106 48-49 -0.20322984528531407 1106 50-51 0.07539495323238299 1106 52-53 0.004048903606850729 1106 54-55 -0.1086701679654496 1106 56-57 0.020059753734052777 1106 58-59 0.23023897150309125 1106 60-61 0.27861473326234787 1106 62-63 0.19691595960535224 1106 64-65 0.37688395631404603 1106 66-67 0.2451015157149321 1106 68-69 0.20088906879057333 1106 70-71 0.13325348450701213 1106 72-73 0.3979257710370767 1106 74-75 0.3456276558787259 1106 76-77 0.53369617827952 1106 78-79 0.7104616990725425 1106 80-81 0.5729201802076034 1106 82-83 0.8501214312073664 1106 84-85 1.1006096503579315 1106 86-87 1.1381021879969389 1106 88-89 1.2991822299150293 1106 90-91 1.233141023481533 1106 92-93 1.3433618496779047 1106 94-95 1.3325280123088596 1106 96-97 1.488766142248707 1106 98-99 1.4517704096867021 1106 100-101 1.6565746107956816 1107 1 0.02478686280725384 1107 2 0.009984313242945575 1107 3 0.03624838201505298 1107 4 0.046753058522384094 1107 5 6.193892920194344E-4 1107 6 -0.010065080560949013 1107 7 0.0569849606209516 1107 8 0.10735789071026858 1107 9 0.0903010273529361 1107 10-11 -0.0025636921221803277 1107 12-13 0.08556093038945534 1107 14-15 0.22076223299064424 1107 16-17 0.1276861324287566 1107 18-19 0.08535889482000414 1107 20-21 0.10909678200577133 1107 22-23 0.08362375063314431 1107 24-25 0.09779101818919145 1107 26-27 0.17359295525833573 1107 28-29 0.0700128189097029 1107 30-31 0.08158472398923777 1107 32-33 0.16062145462922928 1107 34-35 0.28892703499564476 1107 36-37 0.24208502527785214 1107 38-39 0.13875543764162757 1107 40-41 0.22206449896134473 1107 42-43 0.25780890270081613 1107 44-45 0.24487704922744769 1107 46-47 0.388755953624063 1107 48-49 0.4126940166308444 1107 50-51 0.4522818110083193 1107 52-53 0.43414025593650507 1107 54-55 0.45884842006085336 1107 56-57 0.546336083786727 1107 58-59 0.5441223289519073 1107 60-61 0.5962753877709837 1107 62-63 0.8809362261650548 1107 64-65 1.0012482386514847 1107 66-67 1.108942133835452 1107 68-69 1.1827212727550638 1107 70-71 0.9168038572226074 1107 72-73 1.064711821302346 1107 74-75 1.3749148402881808 1107 76-77 1.2599901771047826 1107 78-79 1.4520726135986557 1107 80-81 1.4626956912887863 1107 82-83 1.6047881360767846 1107 84-85 1.880941441255171 1107 86-87 2.1249890935263096 1107 88-89 2.2004448308538613 1107 90-91 2.1224915289516915 1107 92-93 2.0912117510670534 1107 94-95 1.7810927826864464 1107 96-97 1.975020314705482 1107 98-99 1.897274184596089 1107 100-101 1.298920450754805 1108 1 -5.422588066075207E-4 1108 2 0.026848323229572202 1108 3 -0.0019177790230244796 1108 4 0.06067729202278116 1108 5 -0.0045892334349630914 1108 6 -0.012241320832693248 1108 7 0.010437033327754364 1108 8 0.04660420777088348 1108 9 0.03749201049495099 1108 10-11 0.047236325287741465 1108 12-13 0.013680871788395166 1108 14-15 0.13742820511254195 1108 16-17 0.10118137803830507 1108 18-19 -0.04183285695155092 1108 20-21 0.09467340233241117 1108 22-23 0.06798700125083457 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56-57 -0.3141800267043138 2316 58-59 -0.37023817218136656 2316 60-61 -0.1810735496498701 2316 62-63 -0.32685939258711727 2316 64-65 -0.2724469094688331 2316 66-67 -0.2751361748592771 2316 68-69 -0.012352612668806273 2316 70-71 6.981849388303374E-4 2316 72-73 0.228873385948134 2316 74-75 0.3263341810389271 2316 76-77 0.30782718506528184 2316 78-79 0.04753412671414026 2316 80-81 -0.3662597369532037 2316 82-83 0.15281811134484613 2316 84-85 0.41473763565334565 2316 86-87 0.480953215933134 2316 88-89 0.5027551422540384 2316 90-91 0.588518646827584 2316 92-93 0.6530394314784616 2316 94-95 0.9027713681774721 2316 96-97 0.7061308990816855 2316 98-99 0.336701985519813 2316 100-101 -0.10659719869935103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 14.0 4 33.0 5 99.0 6 217.0 7 569.0 8 1280.0 9 2435.0 10 3931.0 11 6137.0 12 8734.0 13 12320.0 14 16440.0 15 22110.0 16 28202.0 17 36333.0 18 35035.0 19 29320.0 20 29965.0 21 32432.0 22 35651.0 23 39845.0 24 44230.0 25 50919.0 26 58132.0 27 67944.0 28 80093.0 29 93141.0 30 109132.0 31 126453.0 32 148933.0 33 176681.0 34 213900.0 35 258570.0 36 294422.0 37 257869.0 38 82553.0 39 1574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54001292255035 19.74306244485672 15.598283313195555 22.11864131939738 2 15.892366867939714 20.94779317489802 39.611558538528186 23.548281418634083 3 19.237888815587915 25.381078455458432 30.009224283101936 25.371808445851713 4 13.24946968616811 17.39849213373012 36.31960745760711 33.03243072249466 5 13.538138067982713 38.09666529627654 33.50321182587094 14.861984809869803 6 31.595688651429487 36.31678077992194 17.23670640504379 14.850824163604779 7 28.50031031101353 33.437684851210754 20.050040508785354 18.011964328990366 8 26.7334815954108 34.9952819404319 19.19763889177561 19.073597572381683 9 26.9825460458821 16.570988891124372 19.95993588411253 36.486529178881 10-11 25.185136996181072 27.098091119617933 26.45019996666183 21.266571917539164 12-13 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29.461626955960558 22.079270377479475 38-39 21.882889980439398 26.420742138861574 29.496558705715813 22.199809174983216 40-41 22.295590940961095 26.186742952026403 29.521677573929335 21.995988533083167 42-43 22.08284712906338 26.308469744202856 29.322638294369785 22.286044832363974 44-45 22.010472061685064 26.53995466485204 29.26349298460825 22.186080288854644 46-47 22.27479059713591 26.74227339804211 28.58557978093239 22.397356223889595 48-49 22.217395623887256 26.731371258756987 28.600844428389653 22.450388688966104 50-51 22.47059112065715 27.01210607856252 28.14308891855053 22.3742138822298 52-53 22.50725479298535 27.00194250953276 28.067953331551415 22.42284936593047 54-55 22.286129617305253 27.04004030509829 28.171896089665545 22.501933987930915 56-57 22.271601325379283 27.22165434637028 27.95814937411952 22.54859495413092 58-59 22.367126403622137 26.82519201663084 28.01310331382233 22.79457826592469 60-61 22.23136273715514 27.05045328686497 28.167302738427146 22.550881237552744 62-63 22.11952190903834 27.018050415459268 28.252144637771647 22.610283037730746 64-65 22.040268551230874 26.933501105107187 28.088761078611217 22.93746926505072 66-67 22.088878201044587 26.959327463933203 27.988783028885656 22.963011306136558 68-69 22.26105239704161 26.848911221666487 27.96103061482388 22.929005766468023 70-71 22.210321994122587 26.896769458726222 27.850565928245157 23.042342618906037 72-73 22.293595982118735 26.895686231473793 27.635903204378366 23.17481458202911 74-75 22.056295780227472 27.233584588953256 27.47603455364057 23.234085077178694 76-77 22.095349657951218 26.934330873430934 27.887357725621882 23.08296174299597 78-79 22.136918447438966 26.983776948526618 27.804365637409585 23.074938966624835 80-81 21.929296017561995 27.213399125683107 27.67117570535525 23.186129151399655 82-83 22.099336700855265 27.134428391107264 27.48215485654153 23.28408005149594 84-85 22.12662480731785 26.929191075844017 27.51039693770484 23.43378717913329 86-87 22.008596750860505 27.036656745206937 27.438725286419796 23.516021217512762 88-89 22.049603205119944 27.04858269133802 27.282116150680213 23.619697952861824 90-91 22.074625959834123 26.892336458232872 27.414425529509185 23.61861205242382 92-93 21.882730534381924 26.74559649224177 27.40673060255508 23.964942370821223 94-95 21.85632911839664 26.772067103665492 27.341975207542575 24.02962857039529 96-97 21.90716774794016 26.710004900960282 27.204943692996196 24.177883658103358 98-99 21.782731576608576 26.641063063594846 27.10902371125321 24.467181648543367 100-101 22.027160637008578 26.445695249580105 27.176807271047892 24.350336842363426 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1428.0 1 992.5 2 714.5 3 869.0 4 755.5 5 737.5 6 1190.5 7 2555.0 8 4015.0 9 4540.5 10 3905.0 11 3022.0 12 2949.0 13 3218.5 14 3498.0 15 3680.5 16 3911.5 17 4236.0 18 4554.0 19 4815.5 20 5192.5 21 5827.0 22 6640.5 23 7790.5 24 9386.0 25 11608.5 26 14515.0 27 17577.5 28 20166.0 29 23083.0 30 26827.5 31 31134.5 32 36413.0 33 40928.5 34 44537.5 35 48368.5 36 52979.0 37 57440.0 38 60627.5 39 63373.0 40 66672.5 41 71783.0 42 76285.5 43 80245.0 44 83138.0 45 85212.0 46 87412.0 47 89350.0 48 90445.0 49 89856.0 50 89091.5 51 86129.5 52 82477.5 53 80615.5 54 79897.5 55 75173.5 56 67558.0 57 61668.5 58 57300.5 59 52396.5 60 44288.5 61 36366.0 62 29919.5 63 23253.5 64 17825.0 65 14264.0 66 10993.0 67 8438.0 68 6464.0 69 4826.0 70 3799.0 71 2947.5 72 2384.5 73 2085.5 74 1668.0 75 1276.5 76 977.0 77 672.0 78 465.5 79 324.0 80 194.0 81 101.5 82 53.5 83 31.0 84 17.0 85 8.5 86 3.5 87 1.5 88 2.5 89 2.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022987540586726835 2 0.01226279289888683 3 0.0018290267374610864 4 0.0 5 4.156878948775196E-4 6 0.0 7 0.0 8 4.156878948775197E-5 9 2.9098152641426374E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015380452110468226 26-27 0.008272189108062641 28-29 1.6627515795100787E-4 30-31 0.0 32-33 2.7019713167038777E-4 34-35 0.009997293871804347 36-37 9.768665529621711E-4 38-39 0.012720049583252102 40-41 0.012325146083118457 42-43 0.00465570442262822 44-45 4.988254738530236E-4 46-47 4.156878948775197E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.313757897550393E-5 66-67 4.3647228962139564E-4 68-69 7.898070002672873E-4 70-71 6.235318423162795E-5 72-73 2.078439474387598E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.235318423162795E-5 82-83 0.0010184353424499233 84-85 0.005611786580846515 86-87 0.0037827598433854285 88-89 0.0 90-91 0.001122357316169303 92-93 4.988254738530236E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.0784394743875983E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2405651.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.95419230664103 #Duplication Level Percentage of deduplicated Percentage of total 1 71.92584305705213 35.21071552832212 2 14.813336651723935 14.503498623030154 3 5.516609204703426 8.101834436629128 4 2.5680421120420864 5.028657096178437 5 1.3732397690758145 3.3612921869232384 6 0.8435727609155079 2.4777853897501143 7 0.5713733936436769 1.9579786093931466 8 0.3761418208935103 1.4730975227672827 9 0.29011512651777915 1.2782116525155172 >10 1.5238053525267894 13.589948909721064 >50 0.12864009075105118 4.342190740311906 >100 0.06477462870981213 5.680271056137291 >500 0.0036558368323161734 1.2220221589056937 >1k 5.951362285164507E-4 0.5060647604009317 >5k 8.501946121663581E-5 0.3107393289870348 >10k+ 1.7003892243327163E-4 0.9556920000268273 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12390 0.5150373017532468 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10572 0.43946524246451374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7466 0.3103525823155562 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2624 0.10907650361586116 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.156878948775197E-5 0.0 12-13 0.0 0.0 0.0 6.235318423162795E-5 0.0 14-15 0.0 0.0 0.0 8.313757897550393E-5 0.0 16-17 0.0 0.0 0.0 8.313757897550393E-5 0.0 18-19 0.0 0.0 0.0 1.0392197371937992E-4 0.0 20-21 0.0 0.0 0.0 2.494127369265118E-4 0.0 22-23 0.0 0.0 0.0 5.403942633407755E-4 0.0 24-25 0.0 0.0 0.0 0.001226279289888683 0.0 26-27 0.0 0.0 0.0 0.0026811869219600017 0.0 28-29 0.0 0.0 0.0 0.007004341028686206 0.0 30-31 0.0 0.0 0.0 0.018123992216659855 0.0 32-33 0.0 0.0 0.0 0.03940721243438886 4.156878948775197E-5 34-35 0.0 0.0 0.0 0.06948223162877741 4.156878948775197E-5 36-37 0.0 0.0 0.0 0.11928164143510427 4.156878948775197E-5 38-39 0.0 0.0 0.0 0.19497840709230058 4.156878948775197E-5 40-41 0.0 0.0 0.0 0.27857324275216977 4.156878948775197E-5 42-43 0.0 0.0 0.0 0.3647661277550235 4.156878948775197E-5 44-45 0.0 0.0 0.0 0.46812692281631874 4.156878948775197E-5 46-47 0.0 0.0 0.0 0.5914823056212227 4.156878948775197E-5 48-49 0.0 0.0 0.0 0.7280565634832318 4.156878948775197E-5 50-51 0.0 0.0 0.0 0.8765194951387378 4.156878948775197E-5 52-53 0.0 0.0 0.0 1.0195992685555801 4.156878948775197E-5 54-55 0.0 0.0 0.0 1.1725516294757634 4.156878948775197E-5 56-57 0.0 0.0 0.0 1.3550801841164826 4.156878948775197E-5 58-59 0.0 0.0 0.0 1.5556495933948855 4.156878948775197E-5 60-61 0.0 0.0 0.0 1.7718696519154276 4.156878948775197E-5 62-63 0.0 0.0 0.0 1.9875701005673725 4.156878948775197E-5 64-65 0.0 0.0 0.0 2.211002344064039 4.156878948775197E-5 66-67 0.0 0.0 0.0 2.466068436360885 4.156878948775197E-5 68-69 0.0 0.0 0.0 2.7565719216960396 4.156878948775197E-5 70-71 0.0 0.0 0.0 3.070582557486518 4.156878948775197E-5 72-73 0.0 0.0 0.0 3.390870080489647 4.156878948775197E-5 74-75 0.0 0.0 0.0 3.7122176076247135 4.156878948775197E-5 76-77 0.0 0.0 0.0 4.06397270427007 4.156878948775197E-5 78-79 0.0 0.0 0.0 4.454137362402111 4.156878948775197E-5 80-81 0.0 0.0 0.0 4.866686813673305 4.156878948775197E-5 82-83 4.156878948775197E-5 0.0 0.0 5.299376343451316 4.156878948775197E-5 84-85 4.156878948775197E-5 0.0 0.0 5.7267450681748935 4.156878948775197E-5 86-87 4.156878948775197E-5 0.0 0.0 6.167686002666223 4.156878948775197E-5 88-89 4.156878948775197E-5 0.0 0.0 6.641632556010826 4.156878948775197E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6430 0.0 61.554462 1 GTATCAA 11515 0.0 43.11991 1 TGGTATC 2355 0.0 32.476574 2 TATCAAC 15720 0.0 31.458168 2 ATCAACG 15835 0.0 31.075832 3 TCAACGC 16070 0.0 30.650312 4 CAACGCA 16430 0.0 29.978731 5 AACGCAG 17000 0.0 28.97356 6 GTGGTAT 3010 0.0 26.993246 1 ACGCAGA 19440 0.0 25.23923 7 CGCAGAG 19665 0.0 24.974604 8 GCAGAGT 20860 0.0 23.06573 9 GAGTACT 11460 0.0 22.774828 12-13 TACATGG 15295 0.0 22.67298 2 GTACATG 15640 0.0 22.420492 1 GTACTTT 11815 0.0 21.86942 14-15 ACATGGG 15325 0.0 21.478989 3 CAGAGTA 20265 0.0 21.211628 10-11 AGAGTAC 20015 0.0 20.978224 10-11 AGTACTT 11805 0.0 20.841827 12-13 >>END_MODULE