FastQCFastQC Report
Thu 26 May 2016
SRR938655_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938655_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932868
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182590.9446584039882702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131330.6794566416330551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99750.5160724891715316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41550.2149655330834801No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29380.15200210257503358No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.13296303731035955No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG25590.13239393481603504No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG23590.12204661673740783No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC19330.10000682922993188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68300.055.0145071
GTATCAA132600.047.3120841
TATCAAC178350.035.0947652
ATCAACG181100.034.535593
TCAACGC183400.034.1284184
CAACGCA186400.033.451565
AACGCAG190650.032.753536
GCGTATC804.4599088E-429.7269841
ACGCAGA213300.029.0772237
CGCAGAG215750.028.7250028
GTACATG149000.027.388731
TACATGG151600.026.5418222
GCAGAGT224000.026.5206419
ACATGGG153300.025.3477553
GAGTACT129400.024.45777912-13
AGTACTT131150.024.18577812-13
CATGGGA94650.023.0649054
TACCGTC1252.2482577E-422.8125867
GTACTTT137800.022.73566614-15
ATACCGT1050.002190712822.6297766
Produced by FastQC (version 0.11.5)