##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938655_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1932868 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.271139570834634 31.0 31.0 34.0 28.0 34.0 2 31.273930242520443 31.0 31.0 34.0 28.0 34.0 3 31.28725086244896 31.0 31.0 34.0 28.0 34.0 4 34.98504295171735 37.0 35.0 37.0 32.0 37.0 5 34.98551375468992 37.0 35.0 37.0 32.0 37.0 6 35.04005602038008 37.0 35.0 37.0 32.0 37.0 7 34.9627574154055 37.0 35.0 37.0 32.0 37.0 8 34.946172216623175 37.0 35.0 37.0 32.0 37.0 9 36.43470531872844 39.0 37.0 39.0 32.0 39.0 10-11 36.2876244006316 39.0 35.0 39.0 32.0 39.0 12-13 36.14753645877525 39.0 35.0 39.0 31.0 39.0 14-15 37.0527314850264 39.0 36.0 41.0 31.0 41.0 16-17 36.941237063265575 39.0 36.0 41.0 31.0 41.0 18-19 37.15170875610751 39.0 36.0 41.0 31.0 41.0 20-21 37.174746542443664 39.0 36.0 41.0 31.0 41.0 22-23 37.08165818876405 39.0 36.0 41.0 31.0 41.0 24-25 36.94077945312354 39.0 36.0 41.0 30.5 41.0 26-27 36.731269026131116 39.0 36.0 41.0 30.0 41.0 28-29 36.51371769825979 39.0 35.5 41.0 30.0 41.0 30-31 36.19450319421709 38.5 35.0 40.0 30.0 41.0 32-33 35.84929493374612 38.0 35.0 40.0 29.0 41.0 34-35 35.55674132946481 38.0 35.0 40.0 27.5 41.0 36-37 35.2556648979651 38.0 34.0 40.0 26.5 41.0 38-39 35.05277675454299 38.0 34.0 40.0 25.5 41.0 40-41 34.887561902830406 38.0 34.0 40.0 25.0 41.0 42-43 34.50743480672244 38.0 33.5 40.0 24.0 41.0 44-45 34.00388567662148 38.0 33.0 40.0 22.5 41.0 46-47 33.437292924296955 38.0 33.0 40.0 19.0 41.0 48-49 32.93990148318457 37.5 32.0 40.0 14.0 41.0 50-51 32.18012507838093 36.5 31.0 39.5 9.0 40.5 52-53 32.1923985497199 37.0 31.0 39.0 8.5 40.0 54-55 32.31002686163774 37.0 31.0 40.0 7.5 41.0 56-57 32.00869485138147 36.5 31.0 40.0 4.5 41.0 58-59 31.67280228137669 36.0 31.0 39.0 2.0 41.0 60-61 31.242283746225816 35.5 30.0 39.0 2.0 40.5 62-63 30.71311155236674 35.0 29.0 39.0 2.0 40.0 64-65 30.248775653588346 35.0 29.0 38.0 2.0 40.0 66-67 29.765136574251322 35.0 28.0 38.0 2.0 40.0 68-69 29.25065007025829 34.0 27.5 37.5 2.0 40.0 70-71 28.682461244120137 34.0 26.0 37.0 2.0 39.0 72-73 28.10278611886585 34.0 26.0 36.0 2.0 39.0 74-75 27.47794805439378 33.0 25.0 36.0 2.0 38.5 76-77 26.794393098752735 33.0 23.5 35.0 2.0 37.5 78-79 26.057701560582515 32.0 21.0 35.0 2.0 37.0 80-81 25.40893040807753 31.5 19.0 35.0 2.0 36.0 82-83 24.719372197170216 31.0 16.5 34.5 2.0 36.0 84-85 24.053127269942905 31.0 8.5 34.0 2.0 35.0 86-87 23.31932573771204 30.5 4.0 34.0 2.0 35.0 88-89 22.541557157550336 29.5 2.0 34.0 2.0 35.0 90-91 21.66930437050021 29.0 2.0 34.0 2.0 35.0 92-93 20.767823255390436 28.5 2.0 34.0 2.0 35.0 94-95 19.80319918380355 26.5 2.0 33.5 2.0 35.0 96-97 18.60104104367189 25.0 2.0 33.0 2.0 35.0 98-99 17.157152738831623 20.5 2.0 32.5 2.0 34.0 100-101 13.733208630904956 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.351127725928027 1101 2 0.3996643066083472 1101 3 0.25289824373376035 1101 4 0.2060956325592258 1101 5 0.21188836416416734 1101 6 0.44698933045373224 1101 7 0.3653388454014035 1101 8 0.5320876743352443 1101 9 0.5954014601734059 1101 10-11 0.6075456699382471 1101 12-13 0.6123338476200715 1101 14-15 0.7448597449114089 1101 16-17 0.6836542104859618 1101 18-19 0.6426576920488145 1101 20-21 0.6564657564213618 1101 22-23 0.6774806556750832 1101 24-25 0.5922932663570677 1101 26-27 0.6809352955449413 1101 28-29 0.6845163203760265 1101 30-31 0.7418089737152016 1101 32-33 0.5599223817103223 1101 34-35 0.5156349224326959 1101 36-37 0.6033948934482041 1101 38-39 0.7763237865527302 1101 40-41 0.6630029336705547 1101 42-43 0.9142145447501093 1101 44-45 0.7764505843752971 1101 46-47 0.7113288672533145 1101 48-49 0.629986285040701 1101 50-51 0.6974612756467877 1101 52-53 0.7483303648262591 1101 54-55 0.6788029404351974 1101 56-57 0.7960075108864828 1101 58-59 0.7567185662140332 1101 60-61 0.8785284723235058 1101 62-63 0.7659362269559402 1101 64-65 0.6594210725452356 1101 66-67 0.6684971453201562 1101 68-69 0.6833899491166306 1101 70-71 0.8860822573077023 1101 72-73 1.172991937906378 1101 74-75 0.9234669665667141 1101 76-77 0.8110971261466737 1101 78-79 1.07337736836946 1101 80-81 1.136226795999427 1101 82-83 0.8132725121984912 1101 84-85 0.5100349002892521 1101 86-87 0.6643271224689364 1101 88-89 0.4589004522342002 1101 90-91 0.30980985107259684 1101 92-93 0.2915370482884967 1101 94-95 0.4006960825082615 1101 96-97 0.25818480766372787 1101 98-99 0.32730651181884696 1101 100-101 0.5362084401055522 1102 1 0.36063040983958317 1102 2 0.1865423337610821 1102 3 0.1558024615875766 1102 4 0.10564113737292047 1102 5 0.34726413026973546 1102 6 0.27959520033586927 1102 7 0.20762925105206165 1102 8 0.36934975849198537 1102 9 0.41263160428680123 1102 10-11 0.5467752163943373 1102 12-13 0.4971592681567003 1102 14-15 0.6991908947899503 1102 16-17 0.7262430078065236 1102 18-19 0.561146378745633 1102 20-21 0.6889390862162657 1102 22-23 0.662805614186972 1102 24-25 0.6116732624511201 1102 26-27 0.4278754951920547 1102 28-29 0.412174676872219 1102 30-31 0.5776750675541251 1102 32-33 0.5421624133881835 1102 34-35 0.5424422727435712 1102 36-37 0.6887419027582808 1102 38-39 0.6308404522165816 1102 40-41 0.7949483888699547 1102 42-43 0.7630060702518904 1102 44-45 0.8883504858525981 1102 46-47 0.8339739949513003 1102 48-49 0.9766836569956538 1102 50-51 0.9403160271063911 1102 52-53 0.8227102953729712 1102 54-55 0.7408636681989336 1102 56-57 0.6901398136587389 1102 58-59 0.7326707590079238 1102 60-61 0.6817437543333789 1102 62-63 0.75373316061766 1102 64-65 0.8160948508184092 1102 66-67 0.5339264350323489 1102 68-69 0.6389310357033224 1102 70-71 0.8669157102509288 1102 72-73 0.7916603587767845 1102 74-75 0.8349980549988167 1102 76-77 0.7264901295225279 1102 78-79 0.8468692836930494 1102 80-81 1.0625520876262726 1102 82-83 1.0375761893371624 1102 84-85 1.3127837877491757 1102 86-87 1.4070806957234687 1102 88-89 1.3006938745752201 1102 90-91 0.900393924950837 1102 92-93 1.2246518680719625 1102 94-95 1.4118924088990674 1102 96-97 1.8133015508415085 1102 98-99 1.9338582543865357 1102 100-101 1.844923144122328 1103 1 0.42561635255250607 1103 2 0.2523119857909535 1103 3 0.2108536516924744 1103 4 0.2794001871405385 1103 5 0.24265186526681504 1103 6 0.23224064226013752 1103 7 0.20021162592432518 1103 8 0.2319643979266388 1103 9 0.3208099222582632 1103 10-11 0.3917524058243913 1103 12-13 0.5561425721155615 1103 14-15 0.5699021612737809 1103 16-17 0.6592160689559563 1103 18-19 0.5848324366047635 1103 20-21 0.5218876943580852 1103 22-23 0.4154343923123207 1103 24-25 0.5989051851821685 1103 26-27 0.44197827252379795 1103 28-29 0.588832014578152 1103 30-31 0.6249854122936398 1103 32-33 0.6774483587507092 1103 34-35 0.5777406361478299 1103 36-37 0.503702706119384 1103 38-39 0.46186234851220576 1103 40-41 0.5976815711224361 1103 42-43 0.5421981250036438 1103 44-45 0.5478667759929579 1103 46-47 0.36768658400446697 1103 48-49 0.38330828321738153 1103 50-51 0.6404083483831542 1103 52-53 0.6744013676948128 1103 54-55 0.6675068799833639 1103 56-57 0.3807810155719409 1103 58-59 0.35929312508221756 1103 60-61 0.5030468570241986 1103 62-63 0.5582559454896128 1103 64-65 0.6182637942856708 1103 66-67 0.6401723588660175 1103 68-69 0.8245057404396405 1103 70-71 0.8221933620215829 1103 72-73 0.8028233482878484 1103 74-75 1.024610538674196 1103 76-77 0.869077340727241 1103 78-79 0.7679487977868895 1103 80-81 0.8569840164865354 1103 82-83 0.6443988019207545 1103 84-85 0.90532661004125 1103 86-87 0.9815492019415899 1103 88-89 0.7755064786641093 1103 90-91 0.7837439806085627 1103 92-93 0.7287555679780127 1103 94-95 0.9655091396085638 1103 96-97 0.9389781984427046 1103 98-99 1.18173194406765 1103 100-101 1.2733128624945493 1104 1 0.40422627713619264 1104 2 0.24567218164021654 1104 3 0.4152438197725097 1104 4 0.4061862804080647 1104 5 0.4204275174251322 1104 6 0.5107046962083572 1104 7 0.39064155570913783 1104 8 0.427088193671878 1104 9 0.46600889518551014 1104 10-11 0.4680254110882771 1104 12-13 0.5756494815412552 1104 14-15 0.6078820323217471 1104 16-17 0.7170404741893677 1104 18-19 0.5851690151611848 1104 20-21 0.5953458148388648 1104 22-23 0.7039665092599208 1104 24-25 0.6050069419070141 1104 26-27 0.7159680137528781 1104 28-29 0.7532914413185026 1104 30-31 0.6402341247162582 1104 32-33 0.5288167927038927 1104 34-35 0.6086849392717895 1104 36-37 0.7032271319779468 1104 38-39 0.7380960464224913 1104 40-41 0.7263538195329531 1104 42-43 0.8702460889425083 1104 44-45 0.7806328180264259 1104 46-47 0.8470911747446692 1104 48-49 0.6158508861114811 1104 50-51 0.7316171018169442 1104 52-53 0.8215966460297395 1104 54-55 0.7689385345090898 1104 56-57 0.8110629225315833 1104 58-59 0.697901017042291 1104 60-61 0.6243843233588713 1104 62-63 0.8016174864574417 1104 64-65 1.0462672198542862 1104 66-67 0.8765566203809776 1104 68-69 0.9980245407134483 1104 70-71 0.8895235706943119 1104 72-73 0.9365801448730409 1104 74-75 0.8874226435456194 1104 76-77 0.8164075792914929 1104 78-79 0.8892424730393955 1104 80-81 0.8653582762976839 1104 82-83 1.001685010270652 1104 84-85 1.307781451421878 1104 86-87 1.5476263579424625 1104 88-89 1.5227643797411012 1104 90-91 1.32406092043394 1104 92-93 1.413515374639637 1104 94-95 1.6789346185455045 1104 96-97 1.8909039598545405 1104 98-99 2.0327757560738817 1104 100-101 1.82272803483413 1105 1 0.346080972244593 1105 2 0.26208825311399764 1105 3 0.3061801971927842 1105 4 0.28746013852383356 1105 5 0.21685214054777902 1105 6 0.3494532299100115 1105 7 0.3671779691363497 1105 8 0.3985676007950687 1105 9 0.48715819178426045 1105 10-11 0.4743705095025703 1105 12-13 0.4855333872818335 1105 14-15 0.5886447606353045 1105 16-17 0.6316850322182077 1105 18-19 0.7168421846441788 1105 20-21 0.5776093012673442 1105 22-23 0.6025040085214926 1105 24-25 0.6702253605833661 1105 26-27 0.6292443079633614 1105 28-29 0.4087830626717519 1105 30-31 0.5540688078411051 1105 32-33 0.5339670513631418 1105 34-35 0.6551850925825988 1105 36-37 0.7228199722224531 1105 38-39 0.7666654455847706 1105 40-41 0.8098221320169543 1105 42-43 0.9307874026895675 1105 44-45 1.0016176922975006 1105 46-47 0.9238266109209547 1105 48-49 0.8215813347636143 1105 50-51 0.755216417685034 1105 52-53 0.9746012566062134 1105 54-55 1.0042389815015227 1105 56-57 0.9111532211923006 1105 58-59 1.1958218974120385 1105 60-61 1.0011142741025019 1105 62-63 1.0554675569241532 1105 64-65 0.881822528487227 1105 66-67 1.1467077088877318 1105 68-69 1.1246030091243888 1105 70-71 1.1625739213400443 1105 72-73 1.1860038931395032 1105 74-75 1.3223801806707485 1105 76-77 1.5078694330301694 1105 78-79 1.3506164473338167 1105 80-81 1.3939262689387633 1105 82-83 1.4933868165251702 1105 84-85 1.2283803762770766 1105 86-87 1.4009983518550406 1105 88-89 1.3399247822312041 1105 90-91 1.0834691407064092 1105 92-93 1.2798891098254543 1105 94-95 1.3936063224334099 1105 96-97 1.3010964563452703 1105 98-99 1.1932633407112903 1105 100-101 1.0000129988412514 1106 1 0.10218957534726414 1106 2 -0.017307899060337917 1106 3 -0.07626788347636904 1106 4 -0.08404261217009434 1106 5 0.11454071510812014 1106 6 -0.1273535961768104 1106 7 0.13851489310252418 1106 8 0.1073335211320412 1106 9 0.011910880997561435 1106 10-11 -0.009575318609350347 1106 12-13 0.08475040172779558 1106 14-15 0.3790609222580912 1106 16-17 0.08165361486472733 1106 18-19 0.01262555727313952 1106 20-21 0.06947046966462977 1106 22-23 0.13615996812126951 1106 24-25 -0.018398165420272505 1106 26-27 -0.10717750030298134 1106 28-29 0.17854679377860094 1106 30-31 -0.12833840428115195 1106 32-33 0.07490412282811576 1106 34-35 -0.29801276179132685 1106 36-37 -0.2820690713140337 1106 38-39 -0.09579165133141032 1106 40-41 0.18870570398265585 1106 42-43 -0.583401286899047 1106 44-45 -0.414158770428557 1106 46-47 -0.31832869338695247 1106 48-49 -0.1754956340594802 1106 50-51 -0.5340933661739662 1106 52-53 -0.5224445273819072 1106 54-55 -0.3551413484723227 1106 56-57 -0.9546610326298293 1106 58-59 -0.7594233507987269 1106 60-61 -0.0689263181576969 1106 62-63 -0.14572359435156912 1106 64-65 -0.0980770672861837 1106 66-67 -0.5046684040210643 1106 68-69 -0.39821184350127936 1106 70-71 -0.3891045476924546 1106 72-73 -0.2859603931121164 1106 74-75 0.12719881213246964 1106 76-77 0.1554419535006879 1106 78-79 0.36400992363046925 1106 80-81 0.3813667134266119 1106 82-83 0.1853602503283831 1106 84-85 0.37420326337457155 1106 86-87 0.18580575677617261 1106 88-89 0.22012673412832484 1106 90-91 0.25610018061397 1106 92-93 0.09488945313218977 1106 94-95 0.024535715316645224 1106 96-97 0.2104839943928205 1106 98-99 0.09549874627690969 1106 100-101 -0.2799510455911207 1107 1 0.14648629031334082 1107 2 0.02063586510289639 1107 3 0.19220153912042903 1107 4 -0.012345869980762814 1107 5 0.14565910976968155 1107 6 0.09930831542583007 1107 7 0.07717096482782893 1107 8 0.031486132956452195 1107 9 0.12199087054053592 1107 10-11 0.21488287297907505 1107 12-13 0.38460041651018173 1107 14-15 0.4794151590896121 1107 16-17 0.5082031855066305 1107 18-19 0.4187217912264032 1107 20-21 0.43730709307513393 1107 22-23 0.449079718953854 1107 24-25 0.3970535540834419 1107 26-27 0.35704115750815646 1107 28-29 0.42751093344265456 1107 30-31 0.4754108916580293 1107 32-33 0.5436750854429704 1107 34-35 0.4714790767639556 1107 36-37 0.5917148557976262 1107 38-39 0.5754549808198774 1107 40-41 0.36065594666332146 1107 42-43 0.61467067358452 1107 44-45 0.5748807812574199 1107 46-47 0.49951465864499767 1107 48-49 0.5481685030057477 1107 50-51 0.41593510502921305 1107 52-53 0.4947243929226559 1107 54-55 0.5499273233492588 1107 56-57 0.4786423757064071 1107 58-59 0.3546453089293493 1107 60-61 0.4842781652913075 1107 62-63 0.5244777213705341 1107 64-65 0.6326742508417489 1107 66-67 0.656546406768296 1107 68-69 0.5631627816964944 1107 70-71 0.6080256888282385 1107 72-73 0.6304850524345547 1107 74-75 0.49245681681188813 1107 76-77 0.6594558399344201 1107 78-79 0.6226842488320763 1107 80-81 0.7580969533437809 1107 82-83 0.583144128036782 1107 84-85 0.8844257504531718 1107 86-87 0.8452239098506347 1107 88-89 0.7900260466767755 1107 90-91 0.7522725856497061 1107 92-93 0.8437071841689914 1107 94-95 0.8833563719529067 1107 96-97 0.9466200108379326 1107 98-99 1.033775287729462 1107 100-101 0.9941989802812667 1108 1 0.43376415411988845 1108 2 0.25558038489207036 1108 3 0.23237235687008706 1108 4 0.22682376985360975 1108 5 0.26787658538503223 1108 6 0.3168928578918795 1108 7 0.32956911421874224 1108 8 0.4646507085563769 1108 9 0.3822735902427752 1108 10-11 0.4898612378002696 1108 12-13 0.46254327660068384 1108 14-15 0.44414418348327445 1108 16-17 0.5598047145929215 1108 18-19 0.6517165156483102 1108 20-21 0.5287888423995355 1108 22-23 0.3979017535821612 1108 24-25 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-1.024744981850457 2316 50-51 -0.9672570206034834 2316 52-53 -1.0323985711468353 2316 54-55 -1.1195506698739024 2316 56-57 -0.8602446267203909 2316 58-59 -0.9068385624756914 2316 60-61 -0.9147471593872822 2316 62-63 -0.9659318600396851 2316 64-65 -0.9702962600751093 2316 66-67 -1.068570641948849 2316 68-69 -1.1320356462987284 2316 70-71 -1.5265098759868962 2316 72-73 -1.3266625337916444 2316 74-75 -1.1817941927490168 2316 76-77 -1.391177118509212 2316 78-79 -1.3419769106285848 2316 80-81 -1.434038081289259 2316 82-83 -1.7449191614667363 2316 84-85 -2.029260263588956 2316 86-87 -1.8991294037765165 2316 88-89 -2.0703418797554285 2316 90-91 -1.9552775895653305 2316 92-93 -2.1718658215860884 2316 94-95 -2.37778815492495 2316 96-97 -2.7924175975877823 2316 98-99 -2.7039261516204647 2316 100-101 -2.392973993376023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6665.0 3 1801.0 4 1841.0 5 2056.0 6 2583.0 7 3193.0 8 4224.0 9 5459.0 10 7160.0 11 9976.0 12 13194.0 13 16799.0 14 20336.0 15 24307.0 16 30088.0 17 36492.0 18 30692.0 19 23280.0 20 23907.0 21 25564.0 22 28113.0 23 31404.0 24 34779.0 25 39500.0 26 44553.0 27 50921.0 28 58563.0 29 66837.0 30 78107.0 31 90643.0 32 107245.0 33 127767.0 34 156328.0 35 192199.0 36 218961.0 37 215145.0 38 97699.0 39 4487.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.6731945827782 19.01279281842994 14.869627080934949 21.44438551785691 2 15.881241571043544 21.257077215973947 39.80621945636209 23.05546175662042 3 18.74885726968125 26.78565046670013 30.020153489723583 24.445338773895042 4 12.677512471259517 17.21854784866565 36.724369496317024 33.379570183757814 5 13.314959010128677 38.27274107490309 32.49804261122657 15.914257303741659 6 30.09194265429717 36.481737045562234 17.740660091813194 15.685660208327402 7 27.77146429362462 34.71981585326221 20.155746409076514 17.352973444036657 8 27.131945019458474 33.1564130164776 19.896290469487454 19.815351494576465 9 28.512739043025803 16.23518392502762 19.873735086897685 35.37834194504889 10-11 25.261844502320102 27.195698371490117 26.08576599594341 21.45669113024637 12-13 26.760960719553573 25.137270238155935 27.015300503736157 21.086468538554335 14-15 23.589219718981152 25.748908783372055 26.87302557000851 23.78884592763828 16-17 21.42997612678422 29.217260754203174 27.063418501664604 22.289344617348007 18-19 21.337344661730484 26.397885927115517 31.58020582296451 20.68456358818949 20-21 22.832759902774338 25.4186457203393 31.319066287847907 20.429528089038456 22-23 22.121000957154216 25.55053186878474 31.382393071849187 20.946074102211856 24-25 21.65783884871382 25.89904765158303 31.538990693911522 20.904122805791626 26-27 21.129349148048522 26.338245688866436 31.512848312959296 21.01955685012575 28-29 21.02476968886807 26.34712762970085 31.796122723902275 20.831979957528805 30-31 21.215206216922383 26.08673706313473 31.48150449024684 21.216552229696045 32-33 21.01458577365575 26.30863826256463 31.153067546744197 21.523708417035422 34-35 21.629462939538346 26.434460122113435 30.987196182072168 20.94888075627605 36-37 21.802556557162784 25.94180843161766 30.83257630461283 21.423058706606728 38-39 21.223063003570683 26.248516312604366 30.94696970677136 21.581450977053596 40-41 21.341764952024185 26.190842790093942 31.000716033747523 21.46667622413435 42-43 21.619156350093956 26.200299444118684 30.452240319172446 21.72830388661491 44-45 21.199048075597222 26.58799156523991 30.338826206533653 21.874134152629214 46-47 21.530272450371665 26.981504876485733 29.496445315238795 21.991777357903807 48-49 21.675922385455628 26.82980463905284 29.33314326900639 22.161129706485145 50-51 21.836791345513795 27.243801435877696 28.714138953285467 22.205268265323046 52-53 22.011517254279127 27.32521483929837 28.50534663137517 22.157921275047336 54-55 21.552584138968744 27.356703486843877 28.617339219594378 22.473373154593002 56-57 21.634260513339548 27.51616297484115 28.544270212806143 22.305306299013157 58-59 21.677654218133082 26.84290733716604 28.67606041945883 22.803378025242047 60-61 21.73759707553404 27.162597347218753 28.934059274876102 22.165746302371108 62-63 21.640414067019414 27.136177879515728 28.80483319655972 22.418574856905135 64-65 21.51936492414041 26.893786227173887 28.82372039897914 22.76312844970656 66-67 21.57394320922689 27.08967014555879 28.683461692991845 22.652924952222474 68-69 21.683163040120622 26.90778502230361 28.753776556194687 22.65527538138108 70-71 21.64438773543089 27.187343044996293 28.509122506218688 22.659146713354136 72-73 21.79070151964484 27.119614619993047 28.26114013340913 22.828543726952976 74-75 21.519095757728817 27.35137767464294 28.174018301373376 22.955508266254864 76-77 21.481630786928925 27.20701074749101 28.385231973947484 22.926126491632576 78-79 21.750452397675343 27.13666872331371 28.26381478276504 22.849064096245908 80-81 21.53576790982457 27.573617292522734 28.067055194472584 22.823559603180104 82-83 21.839639786469228 27.25039678074032 27.8025396554378 23.107423777352647 84-85 21.753862670659224 26.851410869157487 27.900272278668485 23.4944541815148 86-87 21.981826564303756 26.87489554232871 27.773891248115877 23.36938664525166 88-89 21.921243582312027 26.755781094733354 27.86738675886055 23.45558856409407 90-91 21.94078348961774 26.814414238170762 27.843207884010805 23.40159438820069 92-93 22.17620653393448 26.332885165542624 27.84664174315535 23.644266557367548 94-95 22.03738372902516 26.32815860315929 27.97289050769222 23.661567160123333 96-97 22.327376788652 26.228775102849188 27.809406097532886 23.634442010965923 98-99 22.432953007075227 26.140823525028257 27.40318136842933 24.023042099467183 100-101 23.063043846301596 25.568328607292685 27.676175526029812 23.692452020375907 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2759.0 1 1889.5 2 1302.5 3 1662.0 4 1466.5 5 1369.0 6 2296.0 7 4511.5 8 6899.0 9 7468.0 10 6020.0 11 4632.5 12 4399.5 13 4494.5 14 4641.5 15 4834.5 16 5027.5 17 5255.5 18 5499.5 19 5709.0 20 6004.0 21 6617.5 22 7381.5 23 8191.0 24 9274.0 25 10695.0 26 12440.5 27 14527.0 28 16523.0 29 18822.5 30 21739.5 31 25009.5 32 29304.0 33 32891.0 34 34801.0 35 36722.5 36 39937.0 37 43187.0 38 45505.0 39 47395.0 40 49611.0 41 54990.0 42 60127.0 43 63554.0 44 66089.5 45 68572.5 46 69046.5 47 68697.0 48 70921.5 49 70532.5 50 68376.0 51 65646.5 52 63699.5 53 63173.5 54 61854.0 55 58300.0 56 52356.5 57 46947.0 58 43150.5 59 39336.0 60 34244.0 61 29224.0 62 24732.5 63 19215.5 64 14700.0 65 11888.0 66 9326.5 67 7603.0 68 6069.5 69 4521.0 70 3734.0 71 3156.0 72 2688.5 73 2414.5 74 1986.0 75 1616.0 76 1170.5 77 741.0 78 458.0 79 266.0 80 148.5 81 83.5 82 48.5 83 29.5 84 20.0 85 17.0 86 14.5 87 10.0 88 8.0 89 8.0 90 5.0 91 2.5 92 2.5 93 2.0 94 2.0 95 2.5 96 2.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007398332426218448 2 0.10471485895570729 3 0.11304445000900216 4 0.11532085998630015 5 0.15526150776980113 6 0.09172897476703014 7 0.02566134883499546 8 0.02835165153543853 9 0.02188457773629653 10-11 0.049072155987889506 12-13 0.051917668459511984 14-15 0.026204583034123385 16-17 0.029852012656839476 18-19 0.0348445936297771 20-21 0.055539229787031505 22-23 0.02987788095203604 24-25 0.046925087486574354 26-27 0.053728449123271745 28-29 0.05021036097653849 30-31 0.06399816231630924 32-33 0.047442453390505714 34-35 0.058953844752978476 36-37 0.050339702452521326 38-39 0.03127476889265071 40-41 0.036034535208819225 42-43 0.03212842263413746 44-45 0.007838093444560105 46-47 0.01435690383409524 48-49 0.009778215584302705 50-51 0.009441927746747321 52-53 0.010476659554610041 54-55 0.012002888971207552 56-57 0.014874269738026599 58-59 0.01955643116860541 60-61 0.02488529997909842 62-63 0.012106362151993824 64-65 0.009597137517926728 66-67 0.0109940254585414 68-69 0.011433786476883057 70-71 0.030343510265574265 72-73 0.028015363697883145 74-75 0.028662071077797346 76-77 0.023979909647218536 78-79 0.0057168932384415285 80-81 0.005277132220099873 82-83 0.006492942094338569 84-85 0.005458210286475849 86-87 0.013813669634967312 88-89 0.03662950599834029 90-91 0.03380986182191438 92-93 0.02876554425858362 94-95 0.04402783842455874 96-97 0.04094951129616714 98-99 0.025454402473422913 100-101 0.07093086542898946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1932868.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.43506044426661 #Duplication Level Percentage of deduplicated Percentage of total 1 70.1022387630616 27.64486022899744 2 13.470821336256817 10.624453072584076 3 5.74592454168297 6.797726448283885 4 3.0808215024829946 4.859695286736527 5 1.82861525618263 3.605577657843505 6 1.191863103721828 2.8200716121936895 7 0.8592093170029198 2.371807994520102 8 0.6006594999436735 1.8949634949361378 9 0.4385579162451386 1.556510214189479 >10 2.3397343683785445 16.806202369676964 >50 0.20502645118569449 5.650665764652675 >100 0.12531605663620846 9.337696807832156 >500 0.008178082442070588 2.0755680170417232 >1k 0.0026380911103453513 1.8024286341164042 >5k 1.3190455551726757E-4 0.5188659959398324 >10k+ 2.6380911103453513E-4 1.6329064004554603 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18259 0.9446584039882702 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13133 0.6794566416330551 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9975 0.5160724891715316 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4155 0.2149655330834801 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2938 0.15200210257503358 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2570 0.13296303731035955 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2559 0.13239393481603504 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2359 0.12204661673740783 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1933 0.10000682922993188 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.5868295196568003E-5 0.0 14-15 0.0 0.0 0.0 5.1736590393136005E-5 0.0 16-17 0.0 0.0 0.0 7.760488558970401E-5 0.0 18-19 0.0 0.0 0.0 1.5520977117940802E-4 0.0 20-21 0.0 0.0 0.0 1.8107806637597602E-4 0.0 22-23 0.0 0.0 0.0 0.0010088635126661522 0.0 24-25 0.0 0.0 0.0 0.002354014862887688 0.0 26-27 0.0 0.0 0.0 0.005147790744117032 0.0 28-29 0.0 0.0 0.0 0.01280480612230116 0.0 30-31 0.0 0.0 0.0 0.03665537429353686 0.0 32-33 0.0 0.0 0.0 0.07708751968577264 0.0 34-35 0.0 0.0 0.0 0.1364811254570928 0.0 36-37 0.0 0.0 0.0 0.23193513473242872 0.0 38-39 0.0 0.0 0.0 0.3739003387711939 0.0 40-41 0.0 0.0 0.0 0.5305328661864132 0.0 42-43 0.0 0.0 0.0 0.6870360521256496 0.0 44-45 0.0 0.0 0.0 0.8776853877243558 0.0 46-47 0.0 0.0 0.0 1.0998423068724819 0.0 48-49 0.0 0.0 0.0 1.3300960024171335 0.0 50-51 0.0 0.0 0.0 1.5819497244509195 0.0 52-53 0.0 0.0 0.0 1.8261981676969148 0.0 54-55 0.0 0.0 0.0 2.087674895543824 0.0 56-57 0.0 0.0 0.0 2.390980656723584 0.0 58-59 0.0 0.0 0.0 2.714825844289418 0.0 60-61 0.0 0.0 0.0 3.0753005378535936 0.0 62-63 0.0 0.0 0.0 3.429747918636968 0.0 64-65 0.0 0.0 0.0 3.8023807109435306 5.1736590393136005E-5 66-67 0.0 0.0 0.0 4.208435340644058 5.1736590393136005E-5 68-69 0.0 0.0 0.0 4.6567070281053855 5.1736590393136005E-5 70-71 0.0 0.0 0.0 5.136253484459363 5.1736590393136005E-5 72-73 0.0 0.0 0.0 5.625629892988037 5.1736590393136005E-5 74-75 0.0 0.0 0.0 6.114385462431993 5.1736590393136005E-5 76-77 0.0 0.0 0.0 6.635502269166855 5.1736590393136005E-5 78-79 0.0 0.0 0.0 7.2027939828275915 5.1736590393136005E-5 80-81 0.0 0.0 0.0 7.812871856743451 5.1736590393136005E-5 82-83 0.0 0.0 0.0 8.432572736472434 5.1736590393136005E-5 84-85 0.0 0.0 0.0 9.039986175983048 5.1736590393136005E-5 86-87 0.0 0.0 0.0 9.662041070574917 5.1736590393136005E-5 88-89 5.1736590393136005E-5 0.0 0.0 10.316534807343285 5.1736590393136005E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6830 0.0 55.014507 1 GTATCAA 13260 0.0 47.312084 1 TATCAAC 17835 0.0 35.094765 2 ATCAACG 18110 0.0 34.53559 3 TCAACGC 18340 0.0 34.128418 4 CAACGCA 18640 0.0 33.45156 5 AACGCAG 19065 0.0 32.75353 6 GCGTATC 80 4.4599088E-4 29.726984 1 ACGCAGA 21330 0.0 29.077223 7 CGCAGAG 21575 0.0 28.725002 8 GTACATG 14900 0.0 27.38873 1 TACATGG 15160 0.0 26.541822 2 GCAGAGT 22400 0.0 26.520641 9 ACATGGG 15330 0.0 25.347755 3 GAGTACT 12940 0.0 24.457779 12-13 AGTACTT 13115 0.0 24.185778 12-13 CATGGGA 9465 0.0 23.064905 4 TACCGTC 125 2.2482577E-4 22.812586 7 GTACTTT 13780 0.0 22.735666 14-15 ATACCGT 105 0.0021907128 22.629776 6 >>END_MODULE