FastQCFastQC Report
Thu 26 May 2016
SRR938655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938655_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932868
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175450.9077184784475713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135930.7032554732138977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102700.5313347833375067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45850.2372122669525286No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29830.1543302491427247No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28690.1484322778379072No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG27720.143413828569773No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG22550.11666601133652169No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC22070.11418265499765116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68650.057.604591
GTATCAA125650.044.7125321
TATCAAC173000.032.4472662
ATCAACG174250.032.0879443
TCAACGC176000.031.7688874
CAACGCA180350.031.0026325
AACGCAG187450.029.8658986
GTACATG150000.027.2826291
GTGGTAT27000.026.758031
TACATGG152650.026.4976312
ACGCAGA215250.025.9424727
CGCAGAG216400.025.7168358
TGGTATC27150.025.5597922
ACATGGG154050.025.224923
GGACCGA6450.025.030326
GCAGAGT226250.024.1564969
CATGGGG52050.022.7256774
GAGTACT133000.022.3853112-13
AGGACCG8550.022.2245065
AGTACTT134150.022.17571312-13
Produced by FastQC (version 0.11.5)