##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938655_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1932868 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.760856406128095 31.0 31.0 34.0 30.0 34.0 2 31.964780316089872 31.0 31.0 34.0 30.0 34.0 3 32.002261923731986 33.0 31.0 34.0 30.0 34.0 4 35.70892166459375 37.0 35.0 37.0 33.0 37.0 5 35.61269315856023 37.0 35.0 37.0 33.0 37.0 6 35.62854266302717 37.0 35.0 37.0 33.0 37.0 7 35.562777696148935 37.0 35.0 37.0 33.0 37.0 8 35.55134804859928 37.0 35.0 37.0 33.0 37.0 9 37.09508254055631 39.0 37.0 39.0 33.0 39.0 10-11 37.005574876297814 39.0 37.0 39.0 33.0 39.0 12-13 36.892366938663166 39.0 37.0 39.0 32.5 39.0 14-15 37.839202935741085 40.0 37.0 41.0 32.5 41.0 16-17 37.78571325098248 39.5 37.0 41.0 32.5 41.0 18-19 38.08451120304129 40.0 37.0 41.0 33.0 41.0 20-21 38.08316708642287 40.0 37.0 41.0 33.0 41.0 22-23 37.96089851971267 40.0 37.0 41.0 33.0 41.0 24-25 37.81618817218765 40.0 37.0 41.0 32.0 41.0 26-27 37.60066207314726 40.0 36.5 41.0 32.0 41.0 28-29 37.45692023459439 39.5 36.0 41.0 32.0 41.0 30-31 37.12370037684933 39.0 36.0 41.0 31.0 41.0 32-33 36.8314150267892 39.0 35.0 40.0 31.0 41.0 34-35 36.631357133544554 39.0 35.0 40.5 30.0 41.0 36-37 36.51590667339932 39.0 35.0 41.0 30.0 41.0 38-39 36.30457796393753 39.0 35.0 40.0 30.0 41.0 40-41 36.05698733695213 38.5 35.0 40.0 30.0 41.0 42-43 35.7413015270572 38.0 35.0 40.0 29.0 41.0 44-45 35.2794117342726 38.0 34.5 40.0 27.0 41.0 46-47 34.69134519273949 38.0 34.0 40.0 24.5 41.0 48-49 34.18150903217395 38.0 33.0 40.0 23.0 41.0 50-51 33.74646121721711 38.0 33.0 40.0 21.0 41.0 52-53 33.25019246011627 37.0 33.0 40.0 19.0 41.0 54-55 32.909753537230685 37.0 32.0 40.0 17.5 41.0 56-57 32.56782382449293 36.5 31.5 39.5 14.5 41.0 58-59 32.105656206217915 36.0 31.0 39.0 10.5 40.5 60-61 31.71682468745926 35.5 31.0 39.0 8.0 40.0 62-63 31.247553894006213 35.0 30.0 39.0 7.0 40.0 64-65 30.745549618494383 35.0 29.0 38.0 3.5 40.0 66-67 30.249505398195843 34.5 29.0 38.0 2.0 40.0 68-69 29.7833080168951 34.0 28.0 37.0 2.0 39.5 70-71 29.261585374686735 34.0 28.0 36.5 2.0 39.0 72-73 28.80414285921232 34.0 26.5 36.0 2.0 39.0 74-75 28.248836185399107 33.0 26.0 35.5 2.0 38.5 76-77 27.038930490856075 31.5 25.5 34.5 2.0 36.0 78-79 27.721243250961784 33.0 26.0 35.0 2.0 37.0 80-81 27.36980564632453 33.0 26.0 35.0 2.0 36.5 82-83 26.958607623490067 33.0 25.0 35.0 2.0 36.0 84-85 26.44335438322741 32.0 24.5 35.0 2.0 35.5 86-87 25.867533633957414 32.0 23.0 34.5 2.0 35.0 88-89 25.174941072023543 31.0 20.0 34.0 2.0 35.0 90-91 24.606011895276865 31.0 18.0 34.0 2.0 35.0 92-93 23.83130870809595 31.0 9.0 34.0 2.0 35.0 94-95 23.081518499969995 30.5 2.0 34.0 2.0 35.0 96-97 22.07616609101087 30.0 2.0 34.0 2.0 35.0 98-99 20.790402396852762 29.0 2.0 34.0 2.0 35.0 100-101 17.023296469288123 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29801692699023974 1101 2 0.3637907358749466 1101 3 0.3169925100287543 1101 4 0.34145605465947426 1101 5 0.17413199206222174 1101 6 0.26993489162410356 1101 7 0.2683078898404929 1101 8 0.27415938092843106 1101 9 0.3150132843572422 1101 10-11 0.2448186882848944 1101 12-13 0.34595888721791823 1101 14-15 0.5614171938603292 1101 16-17 0.5959033978031485 1101 18-19 0.5410741336245195 1101 20-21 0.42236365880616233 1101 22-23 0.30292964458126903 1101 24-25 0.45895381569879845 1101 26-27 0.45860289603523796 1101 28-29 0.5132482808970806 1101 30-31 0.574257338906051 1101 32-33 0.5103735247943249 1101 34-35 0.5121350244415481 1101 36-37 0.5847049796664407 1101 38-39 0.6884318983410367 1101 40-41 0.799662161374485 1101 42-43 0.8566344580107241 1101 44-45 0.877179684562428 1101 46-47 0.9014380298830886 1101 48-49 0.9958841288619169 1101 50-51 1.0522644378922124 1101 52-53 1.0306191700446803 1101 54-55 1.0773937011240733 1101 56-57 0.8959062034560716 1101 58-59 1.1584394678525953 1101 60-61 1.0624613700241063 1101 62-63 1.229180343758511 1101 64-65 1.164409434981632 1101 66-67 1.0758980735664352 1101 68-69 1.1318684478258199 1101 70-71 1.076673658923177 1101 72-73 0.8266025451969234 1101 74-75 1.0026818327758882 1101 76-77 0.9941824294892676 1101 78-79 1.1181678238957033 1101 80-81 1.0029449869074618 1101 82-83 0.9491201671296423 1101 84-85 1.1464772409765125 1101 86-87 1.0321346420765387 1101 88-89 1.2363030007069327 1101 90-91 1.2301066856987042 1101 92-93 1.51207023331796 1101 94-95 1.47204868675653 1101 96-97 1.6299802968823762 1101 98-99 1.599418393232444 1101 100-101 1.8870045683231638 1102 1 0.24098872247312286 1102 2 0.15476933752492172 1102 3 0.15650303609155714 1102 4 0.0842855956028643 1102 5 0.05225292791691771 1102 6 0.14841785049163292 1102 7 0.11463970931033884 1102 8 0.25667571986399196 1102 9 0.24220598581195674 1102 10-11 0.2644037299108106 1102 12-13 0.28881807572837204 1102 14-15 0.45159026909623634 1102 16-17 0.5093400651208029 1102 18-19 0.37650425360305206 1102 20-21 0.4016263285191499 1102 22-23 0.36333992388352954 1102 24-25 0.46201790183943814 1102 26-27 0.43695966786561513 1102 28-29 0.3157808621278875 1102 30-31 0.4485956889080214 1102 32-33 0.5342687243422901 1102 34-35 0.3691098835063613 1102 36-37 0.5367915532514047 1102 38-39 0.5085173855208183 1102 40-41 0.34572849730159305 1102 42-43 0.4984200818987361 1102 44-45 0.5821890813621735 1102 46-47 0.5953309335933668 1102 48-49 0.7435135840223239 1102 50-51 0.8251862594091577 1102 52-53 0.8740632089172706 1102 54-55 0.8224113468047207 1102 56-57 0.8597607098694553 1102 58-59 1.0033315987765192 1102 60-61 0.9077091372967594 1102 62-63 0.7614768637996967 1102 64-65 0.7168135266147964 1102 66-67 0.8213343818863414 1102 68-69 0.8102735230052609 1102 70-71 0.7504685710376364 1102 72-73 0.7744729120502676 1102 74-75 1.0948029077926371 1102 76-77 0.9407324375734376 1102 78-79 0.9785763552688458 1102 80-81 1.0737436795909332 1102 82-83 1.1433266896616239 1102 84-85 1.1306386501300807 1102 86-87 1.1833335365785977 1102 88-89 1.1372251677760126 1102 90-91 1.180894917698648 1102 92-93 1.5101814614432172 1102 94-95 1.2429469441720364 1102 96-97 1.1471498349147957 1102 98-99 1.4162807999493339 1102 100-101 1.5793085372315367 1103 1 0.19423016655410308 1103 2 0.23626265652270817 1103 3 0.28431916224354126 1103 4 0.18342306843241118 1103 5 0.03401372961803162 1103 6 0.013540359740524366 1103 7 0.19380807720615678 1103 8 0.20904726256465267 1103 9 0.33614990797860855 1103 10-11 0.34691060372786353 1103 12-13 0.287494548710832 1103 14-15 0.4640204415537852 1103 16-17 0.4539405952339166 1103 18-19 0.3689693115001731 1103 20-21 0.3195666957420329 1103 22-23 0.293717965465774 1103 24-25 0.3545967332602089 1103 26-27 0.3725884022564685 1103 28-29 0.3018292401369109 1103 30-31 0.16559522187472453 1103 32-33 0.22548773230995067 1103 34-35 0.3570383658013938 1103 36-37 0.3970767723659918 1103 38-39 0.37672221872480804 1103 40-41 0.37529093850344 1103 42-43 0.4532504926294578 1103 44-45 0.6554231256948952 1103 46-47 0.4866229494310019 1103 48-49 0.5737308614448935 1103 50-51 0.722758803597749 1103 52-53 0.5636452061406132 1103 54-55 0.32534411156839127 1103 56-57 0.43950417036587197 1103 58-59 0.530720403832099 1103 60-61 0.40853562341552063 1103 62-63 0.5250762954428403 1103 64-65 0.4892509966670495 1103 66-67 0.5752456659916625 1103 68-69 0.5757655088991314 1103 70-71 0.37245923101026435 1103 72-73 0.6271452969750868 1103 74-75 0.6006132105534334 1103 76-77 0.49002041132252927 1103 78-79 0.8817312720951023 1103 80-81 0.7367258483684012 1103 82-83 0.7802678418707387 1103 84-85 0.9214051816880264 1103 86-87 0.7210609200205909 1103 88-89 0.6740994094055672 1103 90-91 0.6715191365830719 1103 92-93 0.8356324773762971 1103 94-95 0.7023863956840479 1103 96-97 0.6485633847540804 1103 98-99 0.9717078832194872 1103 100-101 1.1503465993274915 1104 1 0.2329465647450668 1104 2 0.28454972275933343 1104 3 0.2507037525652578 1104 4 0.11264860894588935 1104 5 0.1943587375841247 1104 6 0.16720192193132988 1104 7 0.20470772239377055 1104 8 0.17500290952185082 1104 9 0.2617221064702804 1104 10-11 0.21678159191174728 1104 12-13 0.4262122920203595 1104 14-15 0.4169191720607799 1104 16-17 0.4853174287052511 1104 18-19 0.3282123684465148 1104 20-21 0.3070336282439925 1104 22-23 0.29549038336123346 1104 24-25 0.4521248537110907 1104 26-27 0.3371206148569428 1104 28-29 0.44956675071103547 1104 30-31 0.39656568008189197 1104 32-33 0.33042416798488006 1104 34-35 0.47342430124167834 1104 36-37 0.5285728730167278 1104 38-39 0.4950990790985159 1104 40-41 0.4569454642295767 1104 42-43 0.6461304811511113 1104 44-45 0.6049215459278727 1104 46-47 0.4460915379233654 1104 48-49 0.46540756668158423 1104 50-51 0.6977603729027422 1104 52-53 0.549581094800665 1104 54-55 0.6231200327504212 1104 56-57 0.5074269784951753 1104 58-59 0.71016146223117 1104 60-61 0.7978576238758528 1104 62-63 0.680987792619586 1104 64-65 0.6327083399682074 1104 66-67 0.772198822984933 1104 68-69 0.902911496111539 1104 70-71 0.924563893191209 1104 72-73 0.5583003353408564 1104 74-75 0.6214136756655435 1104 76-77 0.5165903888739933 1104 78-79 0.6555894906200734 1104 80-81 0.5680222736410876 1104 82-83 0.6426517506657241 1104 84-85 0.9538877026195678 1104 86-87 1.058781332643182 1104 88-89 1.0076472642403687 1104 90-91 0.8673286438810877 1104 92-93 1.235717518761117 1104 94-95 1.0964021126471906 1104 96-97 1.1376357909918404 1104 98-99 1.0396051340960533 1104 100-101 1.2896922140915166 1105 1 0.23069416170360668 1105 2 0.2528442032888627 1105 3 0.1843755886661569 1105 4 0.12165568937358273 1105 5 -0.04883637222298631 1105 6 0.07498870457035878 1105 7 0.15983519540966284 1105 8 0.11296885483067598 1105 9 0.2853114360378868 1105 10-11 0.24518558418150604 1105 12-13 0.26247226777193333 1105 14-15 0.29065885874411634 1105 16-17 0.2506334599525246 1105 18-19 0.3002424286174943 1105 20-21 0.3365400518262831 1105 22-23 0.28573461170151404 1105 24-25 0.3343915751383193 1105 26-27 0.1957427304336008 1105 28-29 0.21385503692122398 1105 30-31 0.18164234955222724 1105 32-33 0.291053529958738 1105 34-35 0.24689731150647276 1105 36-37 0.3486847195821454 1105 38-39 0.3133952474534709 1105 40-41 0.30728724887993053 1105 42-43 0.3365626207230221 1105 44-45 0.4022194780604167 1105 46-47 0.2948272160212966 1105 48-49 0.22114040641974952 1105 50-51 0.37976407486652874 1105 52-53 0.2433194030932313 1105 54-55 0.35689468555066384 1105 56-57 0.17542777739921434 1105 58-59 0.3585571841563038 1105 60-61 0.4636047538693582 1105 62-63 0.5515445218389381 1105 64-65 0.41541530252942493 1105 66-67 0.2559684123088779 1105 68-69 0.3937167857564461 1105 70-71 0.5803115014019156 1105 72-73 0.5571860892502833 1105 74-75 0.4408561587943183 1105 76-77 0.2820130344278411 1105 78-79 0.7163268734216928 1105 80-81 0.661471260523868 1105 82-83 0.7833798222970358 1105 84-85 0.7352954615530862 1105 86-87 0.7275920064512285 1105 88-89 0.7227369862273925 1105 90-91 0.6669688557594391 1105 92-93 0.9092896887701443 1105 94-95 1.0859184662691241 1105 96-97 1.0625451407008306 1105 98-99 1.2296319142596062 1105 100-101 1.3792485129153818 1106 1 -0.017954016732737443 1106 2 0.029145260591544542 1106 3 0.09530540828924217 1106 4 0.1135626775941958 1106 5 -0.10011791848747009 1106 6 -0.08149816510957919 1106 7 0.033335006522570154 1106 8 0.13277861092423393 1106 9 -0.20945539303611582 1106 10-11 0.07300904956620258 1106 12-13 -0.0699177243032878 1106 14-15 -0.00797313913969333 1106 16-17 0.10382154854918468 1106 18-19 0.0840532865752266 1106 20-21 0.029231147160793114 1106 22-23 0.12486613593144114 1106 24-25 0.1153781538965788 1106 26-27 0.0882781477892749 1106 28-29 -0.08443023483683731 1106 30-31 -0.02508178474717937 1106 32-33 -0.13446956145413935 1106 34-35 -0.15011039415683314 1106 36-37 0.06467622089395775 1106 38-39 -0.07006634035248283 1106 40-41 -0.09223028889680052 1106 42-43 -0.044564344502880715 1106 44-45 -0.26939012172599774 1106 46-47 -0.885678952147579 1106 48-49 -0.8180062725457802 1106 50-51 -0.09717665452487978 1106 52-53 -0.20780041971125485 1106 54-55 -0.14673725351963895 1106 56-57 -0.3322542237261743 1106 58-59 -0.608681505391278 1106 60-61 -0.40847286463780463 1106 62-63 -0.6621806079132249 1106 64-65 -0.44009332076132424 1106 66-67 -0.5637474063080887 1106 68-69 -0.8482232337696338 1106 70-71 -0.6575903555455866 1106 72-73 -0.2683861866654773 1106 74-75 -0.19490510542794937 1106 76-77 -0.2451708409334863 1106 78-79 -0.005248354658444754 1106 80-81 -0.6578719813804526 1106 82-83 -0.18868075397136153 1106 84-85 -0.10859571650329514 1106 86-87 0.08031492125945405 1106 88-89 0.1855939329092564 1106 90-91 0.1050813197635243 1106 92-93 0.2577918455844532 1106 94-95 0.3528519000504531 1106 96-97 0.5431998745177502 1106 98-99 0.6896524087143092 1106 100-101 1.005191614816205 1107 1 0.07986753096341204 1107 2 0.18247471885646505 1107 3 0.1228436102626489 1107 4 0.12301542581793257 1107 5 -0.028948144432774825 1107 6 0.0438855142653054 1107 7 0.005114679292859137 1107 8 0.08016104852752903 1107 9 0.26303638144931085 1107 10-11 0.07100410049145012 1107 12-13 0.07810551478561223 1107 14-15 0.24744623151934775 1107 16-17 0.17359426523717048 1107 18-19 0.21532032844937277 1107 20-21 0.18880484331991454 1107 22-23 0.1932509297101319 1107 24-25 0.2297508238058441 1107 26-27 0.21885174282609654 1107 28-29 0.21886359904417674 1107 30-31 0.0696442111209592 1107 32-33 0.1295513803830417 1107 34-35 0.19578629178482032 1107 36-37 0.3710003788578007 1107 38-39 0.24485141222105256 1107 40-41 0.42364128114660105 1107 42-43 0.407750185485547 1107 44-45 0.419141090972289 1107 46-47 0.29366659871552514 1107 48-49 0.4117191559567672 1107 50-51 0.43966145432852244 1107 52-53 0.3458696637571066 1107 54-55 0.18465532924948036 1107 56-57 0.2384096815039598 1107 58-59 0.19180805006426738 1107 60-61 0.2949746597055274 1107 62-63 0.4288957219286864 1107 64-65 0.2914179584924206 1107 66-67 0.29448169772000554 1107 68-69 0.3413107107746889 1107 70-71 0.6047766062283841 1107 72-73 0.6565055442833234 1107 74-75 0.5043650128285364 1107 76-77 0.4855587346127237 1107 78-79 0.7498453918920092 1107 80-81 0.8543405308706582 1107 82-83 0.7511118501029159 1107 84-85 0.8156038541514761 1107 86-87 0.9317964916553727 1107 88-89 0.8135756578190261 1107 90-91 1.049201598181785 1107 92-93 1.1210515912142611 1107 94-95 1.4045401922234042 1107 96-97 1.6137998385459973 1107 98-99 1.662953161625179 1107 100-101 1.8004538161998553 1108 1 0.1945034289335119 1108 2 0.15029589609065042 1108 3 0.22506507236001738 1108 4 0.17616860288384117 1108 5 0.13891480071603723 1108 6 0.19832513308550404 1108 7 0.11457641407361052 1108 8 0.17176674939714331 1108 9 0.21356994903019455 1108 10-11 0.27981337437077514 1108 12-13 0.3262041732041965 1108 14-15 0.38623302384366553 1108 16-17 0.36829660123550667 1108 18-19 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-0.679935313235049 2316 74-75 -0.8487623246747376 2316 76-77 -0.854599716541891 2316 78-79 -0.9693734684919875 2316 80-81 -0.7772290490789793 2316 82-83 -0.9091570498239037 2316 84-85 -1.1138105163458398 2316 86-87 -1.2397764948585213 2316 88-89 -1.1692656879006762 2316 90-91 -0.8874344480346643 2316 92-93 -1.0832702193462218 2316 94-95 -1.1379536358386808 2316 96-97 -1.1785616517099804 2316 98-99 -1.105738938534806 2316 100-101 -1.3521565456590423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 51.0 5 121.0 6 305.0 7 643.0 8 1390.0 9 2467.0 10 4242.0 11 6367.0 12 9031.0 13 12339.0 14 15449.0 15 20879.0 16 26449.0 17 33658.0 18 29366.0 19 20428.0 20 18558.0 21 19565.0 22 20925.0 23 23234.0 24 26754.0 25 30765.0 26 36258.0 27 43146.0 28 50956.0 29 60445.0 30 71463.0 31 84520.0 32 102116.0 33 124501.0 34 158692.0 35 206785.0 36 258122.0 37 280333.0 38 128351.0 39 4177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30023209154527 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21.245579108350906 26.455893521958046 31.54711030448018 20.751417065210866 20-21 22.674466130123733 25.532007359012614 31.347691616809836 20.44583489405381 22-23 22.006960640871494 25.671256392055742 31.393607840783748 20.928175126289016 24-25 21.617000690727437 26.03894978385664 31.562078805015663 20.78197072040026 26-27 21.03458882041436 26.449509024435862 31.597955439042796 20.917946716106982 28-29 20.894852514349157 26.358397322939098 31.880533880776007 20.86621628193574 30-31 21.082186029438205 26.165332309617746 31.496871036411143 21.255610624532906 32-33 20.947073493960634 26.35046675663699 31.19825833176183 21.504201417640548 34-35 21.526616441758424 26.545709495819192 31.056715166818442 20.87095889560394 36-37 21.742072268905822 26.01503627107101 30.94366366279341 21.299227797229758 38-39 21.151350262043266 26.28822094881936 31.03384405405342 21.526584735083944 40-41 21.23061409898761 26.20992442240673 31.100350363815465 21.459111114790197 42-43 21.596726891470407 26.248970751093665 30.481288817525908 21.673013539910023 44-45 21.154727743289435 26.694315407787457 30.31973955579195 21.831217293131154 46-47 21.51991237891051 27.013044863901726 29.467868473170437 21.999174284017325 48-49 21.617099563964015 26.83090619742269 29.33754400197013 22.214450236643167 50-51 21.776740968728358 27.187161365307823 28.780298144686085 22.255799521277737 52-53 22.005046387026947 27.354997858105158 28.4408195489811 22.199136205886795 54-55 21.46784211031438 27.36678151550278 28.559979780948204 22.605396593234637 56-57 21.591489951719414 27.518304405681093 28.40706142374958 22.483144218849915 58-59 21.58534581683306 26.993804392684297 28.57671802289102 22.84413176759163 60-61 21.672431847389475 27.38197848999518 28.64825740816238 22.297332254452968 62-63 21.512901143620702 27.374406749186036 28.541983854005647 22.57070825318761 64-65 21.30219063613076 27.15037231577801 28.49208552461196 23.05535152347927 66-67 21.36739621852718 27.326503992520458 28.4141314476502 22.891968341302164 68-69 21.421260565730684 27.123663355833887 28.401227397033445 23.053848681401988 70-71 21.37288804661676 27.32539279733163 28.20212151172326 23.099597644328348 72-73 21.411954731483007 27.2376286722337 27.983463938145754 23.36695265813754 74-75 21.09129033526734 27.492046275462783 27.789858147366942 23.626805241902932 76-77 20.882175426754717 27.235072513880077 28.405264024233063 23.477488035132144 78-79 21.013954790608274 27.22155820947893 28.290970930387516 23.47351606952528 80-81 20.720004140036743 27.6512019044169 28.091287810179317 23.53750614536704 82-83 20.848410320746165 27.296560622492937 27.912371941030962 23.942657115729933 84-85 20.687623735222925 26.89254737513156 27.944833624312697 24.47499526533282 86-87 20.722759405742078 26.946246434646504 27.88063099480639 24.45036316480503 88-89 20.542743735904345 26.90464003537652 27.816160161596954 24.73645606712218 90-91 20.419058146400737 26.827809836672994 28.091199406328254 24.661932610598015 92-93 20.43826953654122 26.39874040848749 28.017450051685174 25.145540003286115 94-95 20.087453644238224 26.394269532555896 28.221356719166106 25.29692010403977 96-97 20.222067009830397 26.353131884440895 28.134698707818025 25.29010239791069 98-99 20.171553072695104 26.2553951658072 27.83046264096796 25.74258912052974 100-101 19.94947149460111 25.98282858728457 28.243235376680566 25.824464541433763 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2793.0 1 1923.5 2 1333.0 3 1677.5 4 1519.0 5 1465.0 6 2382.0 7 4679.5 8 6825.0 9 7066.0 10 5664.0 11 4394.5 12 4196.0 13 4364.5 14 4639.5 15 4867.5 16 5038.0 17 5203.0 18 5423.5 19 5750.5 20 6092.5 21 6541.0 22 7304.0 23 8153.0 24 9138.5 25 10562.5 26 12181.0 27 14321.5 28 16351.0 29 18735.0 30 22057.5 31 25262.0 32 29563.5 33 33002.5 34 34649.0 35 36655.5 36 40161.5 37 43782.0 38 46224.0 39 47838.5 40 49591.0 41 55518.0 42 61232.5 43 64771.0 44 67562.5 45 69545.0 46 70078.5 47 70088.0 48 72658.0 49 72113.5 50 69052.5 51 65811.0 52 63167.0 53 62789.5 54 62241.0 55 58624.5 56 51906.5 57 46007.5 58 42451.0 59 38596.5 60 33223.0 61 28101.5 62 23915.5 63 18466.5 64 13687.0 65 10990.0 66 8619.0 67 7193.0 68 5643.0 69 4018.5 70 3318.0 71 2846.0 72 2465.5 73 2272.0 74 1914.0 75 1556.0 76 1072.5 77 582.0 78 336.0 79 205.0 80 105.0 81 60.5 82 35.5 83 19.5 84 11.0 85 9.5 86 8.0 87 3.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.173659039313601E-4 2 0.05008101950055565 3 0.0 4 0.0 5 0.04687335089618122 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006363600618355728 26-27 0.00168143918777692 28-29 1.0347318078627201E-4 30-31 0.002043595320528872 32-33 4.39761018341656E-4 34-35 0.011795942609635008 36-37 0.024523143846346466 38-39 0.0039578491650749045 40-41 0.0010347318078627202 42-43 0.001371019645418104 44-45 0.00879522036683312 46-47 0.0 48-49 0.0 50-51 7.50180560700472E-4 52-53 0.0 54-55 0.0019401221397426004 56-57 0.0 58-59 0.02330733397210777 60-61 0.0 62-63 0.00985582046989241 64-65 0.015598582003530505 66-67 0.006674020160714545 68-69 0.016710918696982928 70-71 5.1736590393136005E-5 72-73 7.7604885589704E-5 74-75 0.022764099772979843 76-77 0.04977059995819684 78-79 0.030653929807933085 80-81 0.026799553823644448 82-83 0.0362932181607849 84-85 0.016245289383444706 86-87 0.010631869325789448 88-89 0.08502908631111902 90-91 0.025195719521457233 92-93 0.02563548053979889 94-95 0.02742039290836208 96-97 0.0387248379092623 98-99 0.05411647355122026 100-101 0.01528816246117169 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1932868.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.88079545900922 #Duplication Level Percentage of deduplicated Percentage of total 1 67.27490708927711 24.811520878834777 2 14.538953087296843 10.72416310001451 3 6.331670601918303 7.005511451495121 4 3.3755968665648015 4.979787903513954 5 2.0601430605524804 3.798985741626664 6 1.326664045991135 2.9357055193812385 7 0.9254684617711206 2.389240912964121 8 0.6700732490018896 1.9770267551193952 9 0.5061146235604287 1.6799318919311055 >10 2.601966448739513 17.393511597741195 >50 0.23053935346256407 5.884977554563125 >100 0.1452601394683433 10.100001216512226 >500 0.009271513090012942 2.264943179707303 >1k 0.002950026892276845 1.9093765799330455 >5k 0.0 0.0 >10k+ 4.214324131824065E-4 2.1453157166621684 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17545 0.9077184784475713 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13593 0.7032554732138977 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10270 0.5313347833375067 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4585 0.2372122669525286 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2983 0.1543302491427247 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2869 0.1484322778379072 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2772 0.143413828569773 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2255 0.11666601133652169 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2207 0.11418265499765116 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.5868295196568003E-5 0.0 14-15 0.0 0.0 0.0 5.1736590393136005E-5 0.0 16-17 0.0 0.0 0.0 1.0347318078627201E-4 0.0 18-19 0.0 0.0 0.0 2.5868295196568E-4 0.0 20-21 0.0 0.0 0.0 2.84551247162248E-4 0.0 22-23 0.0 0.0 0.0 0.0011640732838455602 0.0 24-25 0.0 0.0 0.0 0.0025092246340670966 0.0 26-27 0.0 0.0 0.0 0.005251263924903304 0.0 28-29 0.0 0.0 0.0 0.013011752483873705 0.0 30-31 0.0 0.0 0.0 0.03706926701668195 0.0 32-33 0.0 0.0 0.0 0.07812225149363536 0.0 34-35 0.0 0.0 0.0 0.13862819395840792 0.0 36-37 0.0 0.0 0.0 0.23493585697523062 0.0 38-39 0.0 0.0 0.0 0.37827208065941387 0.0 40-41 0.0 0.0 0.0 0.5367153887383929 0.0 42-43 0.0 0.0 0.0 0.6947965406846199 0.0 44-45 0.0 0.0 0.0 0.886713422747958 0.0 46-47 0.0 0.0 0.0 1.1100344151799295 0.0 48-49 0.0 0.0 0.0 1.3414521840084268 0.0 50-51 0.0 0.0 0.0 1.593900876831734 0.0 52-53 0.0 0.0 0.0 1.8370887199746697 0.0 54-55 0.0 0.0 0.0 2.099031077135117 0.0 56-57 0.0 0.0 0.0 2.403242228646757 0.0 58-59 0.0 0.0 0.0 2.7281221480204545 0.0 60-61 0.0 0.0 0.0 3.0890624708981678 0.0 62-63 0.0 0.0 0.0 3.444622188374995 0.0 64-65 0.0 0.0 0.0 3.8167634830728225 0.0 66-67 0.0 0.0 0.0 4.223620029924444 0.0 68-69 0.0 0.0 0.0 4.675539147008487 0.0 70-71 0.0 0.0 0.0 5.160750760010513 0.0 72-73 0.0 0.0 0.0 5.65620621791038 0.0 74-75 0.0 0.0 0.0 6.150859758659154 0.0 76-77 0.0 0.0 0.0 6.6816771760927285 0.0 78-79 0.0 0.0 0.0 7.25812626625305 0.0 80-81 0.0 0.0 0.0 7.88041397550169 0.0 82-83 0.0 0.0 0.0 8.513643973618478 0.0 84-85 0.0 0.0 0.0 9.136112760933493 0.0 86-87 0.0 0.0 0.0 9.775292466945492 0.0 88-89 0.0 5.1736590393136005E-5 0.0 10.452576171782036 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6865 0.0 57.60459 1 GTATCAA 12565 0.0 44.712532 1 TATCAAC 17300 0.0 32.447266 2 ATCAACG 17425 0.0 32.087944 3 TCAACGC 17600 0.0 31.768887 4 CAACGCA 18035 0.0 31.002632 5 AACGCAG 18745 0.0 29.865898 6 GTACATG 15000 0.0 27.282629 1 GTGGTAT 2700 0.0 26.75803 1 TACATGG 15265 0.0 26.497631 2 ACGCAGA 21525 0.0 25.942472 7 CGCAGAG 21640 0.0 25.716835 8 TGGTATC 2715 0.0 25.559792 2 ACATGGG 15405 0.0 25.22492 3 GGACCGA 645 0.0 25.03032 6 GCAGAGT 22625 0.0 24.156496 9 CATGGGG 5205 0.0 22.725677 4 GAGTACT 13300 0.0 22.38531 12-13 AGGACCG 855 0.0 22.224506 5 AGTACTT 13415 0.0 22.175713 12-13 >>END_MODULE