FastQCFastQC Report
Thu 26 May 2016
SRR938654_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938654_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923431
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187040.9724289563805512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135130.7025466471113339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101410.5272349255055159No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40600.21108113574128728No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28260.14692494817854135No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26540.13798259464467402No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG25780.1340313221529652No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG25480.13247160932729066No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC19380.10075744853857507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66150.058.799491
GTATCAA132000.047.7524221
TATCAAC178150.035.2652132
ATCAACG181000.034.6836783
TCAACGC182800.034.290164
CAACGCA185300.033.8001255
AACGCAG189400.033.0892266
ACGCAGA213250.029.2502577
CGCAGAG214500.029.0362638
GTACATG149750.028.072121
TACATGG151950.027.271982
GCAGAGT221300.027.198069
TGGTATC25850.027.02452
GTGGTAT26250.026.2978381
ACATGGG153750.025.8399733
CATGGGA92850.024.9769174
AGTACTT135900.024.41494812-13
GAGTACT134150.024.41475912-13
GTACTTT142100.022.89930314-15
AGAGTAC215350.022.5890410-11
Produced by FastQC (version 0.11.5)