##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938654_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1923431 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20664843189072 31.0 31.0 34.0 28.0 34.0 2 31.262843845191224 31.0 31.0 34.0 28.0 34.0 3 31.298449489479996 31.0 31.0 34.0 28.0 34.0 4 34.968987190078565 37.0 35.0 37.0 32.0 37.0 5 35.00915447447816 37.0 35.0 37.0 32.0 37.0 6 35.076089550391984 37.0 35.0 37.0 32.0 37.0 7 35.01192192493518 37.0 35.0 37.0 32.0 37.0 8 34.98930452925008 37.0 35.0 37.0 32.0 37.0 9 36.48387490895176 39.0 37.0 39.0 32.0 39.0 10-11 36.34415375441074 39.0 35.0 39.0 32.0 39.0 12-13 36.20044649379156 39.0 35.0 39.0 32.0 39.0 14-15 37.13151576531729 39.0 36.0 41.0 31.0 41.0 16-17 37.04109245405736 39.0 36.0 41.0 31.0 41.0 18-19 37.119974670263716 39.0 36.0 41.0 31.0 41.0 20-21 37.22231886665027 39.0 36.0 41.0 31.0 41.0 22-23 37.13663058357695 39.0 36.0 41.0 31.0 41.0 24-25 37.0140735487782 39.0 36.0 41.0 31.0 41.0 26-27 36.82353773023311 39.0 36.0 41.0 30.0 41.0 28-29 36.6289206631275 39.0 36.0 41.0 30.0 41.0 30-31 36.33842362944135 39.0 35.0 40.0 30.0 41.0 32-33 35.98622175685013 38.0 35.0 40.0 29.5 41.0 34-35 35.688645446600376 38.0 35.0 40.0 28.5 41.0 36-37 35.375118733138855 38.0 34.5 40.0 27.0 41.0 38-39 35.09161025272027 38.0 34.0 40.0 26.0 41.0 40-41 34.926515429979034 38.0 34.0 40.0 25.0 41.0 42-43 34.65505885056443 38.0 34.0 40.0 24.5 41.0 44-45 34.22614562206807 38.0 33.0 40.0 23.5 41.0 46-47 33.67419210774912 38.0 33.0 40.0 20.0 41.0 48-49 33.176695966738606 38.0 32.5 40.0 16.0 41.0 50-51 32.37618999589795 36.5 31.5 39.5 13.0 40.5 52-53 32.416415509576375 37.0 31.5 39.5 10.5 40.0 54-55 32.54206467505203 37.0 32.0 40.0 8.5 41.0 56-57 32.27278519478993 37.0 31.0 40.0 7.5 41.0 58-59 31.975810153834477 36.0 31.0 39.5 4.0 41.0 60-61 31.562261656383825 36.0 31.0 39.0 2.0 41.0 62-63 31.082071049078444 35.0 30.0 39.0 2.0 40.0 64-65 30.62785018022482 35.0 29.0 38.5 2.0 40.0 66-67 30.133386640851686 35.0 29.0 38.0 2.0 40.0 68-69 29.620403851242912 34.5 28.0 37.5 2.0 40.0 70-71 29.050893429501762 34.0 27.5 37.0 2.0 39.0 72-73 28.55834521747856 34.0 26.0 36.0 2.0 39.0 74-75 28.01883457217857 34.0 26.0 36.0 2.0 39.0 76-77 27.36304811558096 33.0 25.5 35.0 2.0 37.5 78-79 26.63069691608381 33.0 23.5 35.0 2.0 37.0 80-81 26.028608252648525 32.0 21.5 35.0 2.0 36.5 82-83 25.42369234976456 32.0 19.5 35.0 2.0 36.0 84-85 24.807771893039053 31.0 17.5 34.5 2.0 35.5 86-87 24.17180366750874 31.0 9.5 34.0 2.0 35.0 88-89 23.427096163054458 30.5 4.5 34.0 2.0 35.0 90-91 22.65719565713561 30.0 2.0 34.0 2.0 35.0 92-93 21.86754580746593 29.0 2.0 34.0 2.0 35.0 94-95 20.964210309597796 29.0 2.0 34.0 2.0 35.0 96-97 19.793009471096184 27.5 2.0 34.0 2.0 35.0 98-99 18.40547516391282 25.0 2.0 33.0 2.0 35.0 100-101 15.13477556512295 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5695737426766527 1101 2 0.6416039095875092 1101 3 0.5549369253265297 1101 4 0.6085268265063775 1101 5 0.473736452622866 1101 6 0.6101622289914914 1101 7 0.38696431547599985 1101 8 0.6640947170889291 1101 9 0.5493446026913915 1101 10-11 0.7207357250272537 1101 12-13 0.6605158923429784 1101 14-15 0.8223401703845781 1101 16-17 0.9083416925529306 1101 18-19 0.8352571417252008 1101 20-21 0.8374438721240764 1101 22-23 0.9295160331531562 1101 24-25 0.965698603592891 1101 26-27 1.007065677365901 1101 28-29 1.0420139154771277 1101 30-31 0.9972257578086854 1101 32-33 0.9761528594912079 1101 34-35 0.9935655992019221 1101 36-37 1.0213809653573875 1101 38-39 1.0396768065010988 1101 40-41 0.9957312224507291 1101 42-43 0.9149400436426234 1101 44-45 0.9786187148185448 1101 46-47 1.0223131621433623 1101 48-49 1.1184822112720028 1101 50-51 0.8632980764507963 1101 52-53 0.9645123727975502 1101 54-55 1.1208136837167118 1101 56-57 1.097846230278897 1101 58-59 1.0974063501232756 1101 60-61 1.0201169875243856 1101 62-63 1.0149838342533037 1101 64-65 0.9896747857692567 1101 66-67 0.9952555166510706 1101 68-69 1.121196801962462 1101 70-71 1.316955763854029 1101 72-73 1.2913283890781635 1101 74-75 1.0657342201388893 1101 76-77 1.2581764920422387 1101 78-79 1.2483610169955988 1101 80-81 1.2129163705648658 1101 82-83 0.9977388635501292 1101 84-85 1.172538386523506 1101 86-87 1.4990929619181514 1101 88-89 1.3831531656651954 1101 90-91 1.310833427289996 1101 92-93 1.4256715731114404 1101 94-95 1.160830030792468 1101 96-97 1.1908808961277657 1101 98-99 1.2937718127053763 1101 100-101 1.2807724835537098 1102 1 0.5868713175394475 1102 2 0.629432935568186 1102 3 0.6547861511457 1102 4 0.6207650357773176 1102 5 0.5794135464435612 1102 6 0.6622953283136326 1102 7 0.47546994407683485 1102 8 0.6204356980547345 1102 9 0.6905699843124111 1102 10-11 0.750745117060319 1102 12-13 0.7866037621978279 1102 14-15 0.8771994327198058 1102 16-17 0.8058652405676057 1102 18-19 0.9018928499841294 1102 20-21 0.8522121125601245 1102 22-23 0.8286016685680906 1102 24-25 0.7160048201015456 1102 26-27 0.7444909434676958 1102 28-29 0.7596478059456757 1102 30-31 0.6658282539435305 1102 32-33 0.7858621175414982 1102 34-35 0.8663251499171665 1102 36-37 0.9394349542193225 1102 38-39 1.0028487845204026 1102 40-41 0.9709574708142554 1102 42-43 1.1396029790431328 1102 44-45 1.0532987899826765 1102 46-47 0.948921530389093 1102 48-49 0.7256719240694807 1102 50-51 0.776177215337917 1102 52-53 0.8195009149352401 1102 54-55 0.922950615099829 1102 56-57 0.9199072659526024 1102 58-59 0.9296639774922788 1102 60-61 1.0388508885136893 1102 62-63 0.6141870094293509 1102 64-65 0.6898217627260266 1102 66-67 0.6829784293797125 1102 68-69 0.6253648650089261 1102 70-71 0.6623733853425549 1102 72-73 0.801228170232644 1102 74-75 0.8005019519559333 1102 76-77 0.8970655357618718 1102 78-79 0.8820846167082657 1102 80-81 0.8700641397131541 1102 82-83 0.8102218370064911 1102 84-85 0.511675260077336 1102 86-87 0.5534108585486521 1102 88-89 0.8183599819959042 1102 90-91 1.0809620242888691 1102 92-93 0.7855102851487814 1102 94-95 0.5020160118592969 1102 96-97 0.3927310500024319 1102 98-99 0.6072457222924257 1102 100-101 0.4368404824305667 1103 1 0.5534322240624405 1103 2 0.543255212724322 1103 3 0.5398490412439223 1103 4 0.4442863839934077 1103 5 0.5555301622149287 1103 6 0.50293241485614 1103 7 0.4114652094074174 1103 8 0.4665013618212299 1103 9 0.627103101806938 1103 10-11 0.5718430225121978 1103 12-13 0.6036856066774163 1103 14-15 0.7960632560111662 1103 16-17 0.7443973098393002 1103 18-19 0.7066978262289894 1103 20-21 0.6375104602023711 1103 22-23 0.6779728003019514 1103 24-25 0.6466899403756301 1103 26-27 0.7123707982868694 1103 28-29 0.6900556575403556 1103 30-31 0.7396774171646499 1103 32-33 0.7417330487258624 1103 34-35 0.8308061323937821 1103 36-37 0.8278116548823462 1103 38-39 0.7081818635408297 1103 40-41 0.90989587365506 1103 42-43 1.1772047014787006 1103 44-45 1.1588612740744892 1103 46-47 0.9007817987524618 1103 48-49 1.0035995050891842 1103 50-51 1.037378869985794 1103 52-53 1.0238112896585463 1103 54-55 0.9845456477537411 1103 56-57 1.219094729678659 1103 58-59 1.1810495029875803 1103 60-61 1.1041384202237303 1103 62-63 1.1068628632042241 1103 64-65 0.92062822689768 1103 66-67 0.9937298782108002 1103 68-69 1.01909440519767 1103 70-71 1.2561376372798385 1103 72-73 1.3162813240289175 1103 74-75 1.27234944796621 1103 76-77 1.1262566440579498 1103 78-79 1.0712147357096917 1103 80-81 1.0907292770126773 1103 82-83 1.0789171071136217 1103 84-85 1.033437988754475 1103 86-87 1.1892736565184236 1103 88-89 1.1803853618131015 1103 90-91 1.2932857092174253 1103 92-93 1.2901638187131788 1103 94-95 1.0485973135793145 1103 96-97 0.9005575514573181 1103 98-99 0.8337990975764846 1103 100-101 0.5586335716191488 1104 1 0.47967027444004273 1104 2 0.5123049571298139 1104 3 0.5031166355667871 1104 4 0.5154794157612628 1104 5 0.3702419041185294 1104 6 0.490010606698732 1104 7 0.36418841059349916 1104 8 0.3748691090374905 1104 9 0.4926628615017705 1104 10-11 0.528419291604564 1104 12-13 0.3792100001230807 1104 14-15 0.5769960601377377 1104 16-17 0.5249949840064971 1104 18-19 0.5322753462672907 1104 20-21 0.5438496673188666 1104 22-23 0.6957626413854925 1104 24-25 0.5001532073521986 1104 26-27 0.6242376420421749 1104 28-29 0.5224352346897376 1104 30-31 0.5891251477016297 1104 32-33 0.6932210566330781 1104 34-35 0.5980942051648555 1104 36-37 0.6890023700836636 1104 38-39 0.5721757786927171 1104 40-41 0.6114251164466822 1104 42-43 0.480650053674033 1104 44-45 0.6168799919321302 1104 46-47 0.551497000062092 1104 48-49 0.3175004487039601 1104 50-51 0.3193243225656772 1104 52-53 0.4167026735753936 1104 54-55 0.49721865666479204 1104 56-57 0.5064919899526359 1104 58-59 0.41838498815382863 1104 60-61 0.44577700495601036 1104 62-63 0.6122768336448381 1104 64-65 0.4856581106060389 1104 66-67 0.5561543967510545 1104 68-69 0.638763445058057 1104 70-71 0.5797298597937761 1104 72-73 0.46227397279109894 1104 74-75 0.5712498510487869 1104 76-77 0.6661998109588474 1104 78-79 0.633061965868297 1104 80-81 0.8102942258165591 1104 82-83 0.8046496826723022 1104 84-85 0.7917926087603462 1104 86-87 0.900008780300297 1104 88-89 0.8846427477994148 1104 90-91 0.9384000703855939 1104 92-93 0.9835983602646579 1104 94-95 0.5520272219977151 1104 96-97 0.7706863525569148 1104 98-99 0.5523478458583604 1104 100-101 0.5078661703434939 1105 1 0.5401664741512633 1105 2 0.5784213331861636 1105 3 0.6007552526574607 1105 4 0.5698866513351888 1105 5 0.5307570136562632 1105 6 0.5681521665395408 1105 7 0.564219487207616 1105 8 0.49578469645145873 1105 9 0.5827826970302539 1105 10-11 0.5988566363131156 1105 12-13 0.6375617668725653 1105 14-15 0.7671888963918363 1105 16-17 0.6340690621524487 1105 18-19 0.8135653189286671 1105 20-21 0.8168772184963302 1105 22-23 0.8651161158828771 1105 24-25 0.7774738754994104 1105 26-27 0.9401051853039064 1105 28-29 0.9600260641568212 1105 30-31 0.9350424575222789 1105 32-33 0.7862229549451172 1105 34-35 1.01909041587016 1105 36-37 1.096618925710068 1105 38-39 1.0266592399239087 1105 40-41 1.0589126748069475 1105 42-43 1.209615217082785 1105 44-45 1.1263126238428285 1105 46-47 1.1334137821666488 1105 48-49 1.0646269613068498 1105 50-51 0.7878409272989941 1105 52-53 0.8647825932452733 1105 54-55 0.8909565723328328 1105 56-57 1.2121391384534093 1105 58-59 1.3508481762919438 1105 60-61 1.3811040789901874 1105 62-63 1.2813679159758813 1105 64-65 1.477243970746617 1105 66-67 1.4749440212663991 1105 68-69 1.2726180446168271 1105 70-71 1.2207543619449517 1105 72-73 1.270298628859468 1105 74-75 1.311700022190042 1105 76-77 1.1058582613791863 1105 78-79 0.9951518681675289 1105 80-81 1.0364491544649432 1105 82-83 1.0760465911355475 1105 84-85 0.8459594403382908 1105 86-87 0.8978963034794916 1105 88-89 1.1394714233053271 1105 90-91 1.1226345522343557 1105 92-93 1.0935578287372856 1105 94-95 1.196324003662511 1105 96-97 1.0446954556594434 1105 98-99 1.0633639192108966 1105 100-101 1.0392534040117791 1106 1 0.25959620835583763 1106 2 0.15031164465003855 1106 3 -0.04311426365300264 1106 4 0.2190223633037789 1106 5 0.34142084921614924 1106 6 -0.17055100118247424 1106 7 0.09008493380207483 1106 8 0.22048231149677378 1106 9 0.29097731876995425 1106 10-11 0.041468585100417954 1106 12-13 0.18255951294082706 1106 14-15 0.37713484415959897 1106 16-17 0.12571982490823075 1106 18-19 -0.34552855838161634 1106 20-21 0.08783773045480814 1106 22-23 0.1311209270792304 1106 24-25 0.24604901147257863 1106 26-27 -0.16322936978681923 1106 28-29 0.11907781190183186 1106 30-31 0.3524221670810519 1106 32-33 0.28834923410404656 1106 34-35 0.19476986043397204 1106 36-37 0.08471732352270323 1106 38-39 0.17466166658686433 1106 40-41 0.263970329117015 1106 42-43 -0.1476144269645161 1106 44-45 0.16424776959005527 1106 46-47 -0.11533433780149238 1106 48-49 0.4407449746450638 1106 50-51 -0.1315871217229656 1106 52-53 0.14202967893260166 1106 54-55 0.1364657713234081 1106 56-57 -0.47355618895651475 1106 58-59 -0.7324394464630721 1106 60-61 0.038596190229089444 1106 62-63 0.25647271326947774 1106 64-65 0.3179883159057191 1106 66-67 -0.1831509640514497 1106 68-69 -0.4672740194369638 1106 70-71 -0.5586859889100673 1106 72-73 0.049220364766075164 1106 74-75 0.008282893151399406 1106 76-77 0.21494392342424007 1106 78-79 0.39333018576546763 1106 80-81 0.3674725237347971 1106 82-83 0.30571465229082406 1106 84-85 0.16687045005757994 1106 86-87 0.39313861167411446 1106 88-89 0.0719486838724741 1106 90-91 0.28391116232101865 1106 92-93 0.34071050206884834 1106 94-95 0.21952012100177853 1106 96-97 0.5921590451826617 1106 98-99 0.58574230168837 1106 100-101 0.12193225454872092 1107 1 0.27067452197142217 1107 2 0.2577554549514183 1107 3 0.18652176265128873 1107 4 0.3017837903088534 1107 5 0.39121869975900836 1107 6 0.29875970187895007 1107 7 0.2846691601257234 1107 8 0.17005642380551222 1107 9 0.0728150078025891 1107 10-11 0.23164686914591215 1107 12-13 0.23964076263007428 1107 14-15 0.2324008048078383 1107 16-17 0.1940000236819941 1107 18-19 0.23023038525990103 1107 20-21 0.34831940877941747 1107 22-23 0.33351322525033567 1107 24-25 0.31623373289885137 1107 26-27 0.3558611277494421 1107 28-29 0.454668807942582 1107 30-31 0.5900544146951674 1107 32-33 0.36859288634941123 1107 34-35 0.47163140179414853 1107 36-37 0.4245083179933573 1107 38-39 0.6077481028162381 1107 40-41 0.5721295784032776 1107 42-43 0.5519066947098281 1107 44-45 0.8624722562351081 1107 46-47 0.6323402238154898 1107 48-49 0.6769242321481457 1107 50-51 0.7941839901383005 1107 52-53 0.5540804033996167 1107 54-55 0.6254782614121908 1107 56-57 0.5408793757404737 1107 58-59 0.730267969347242 1107 60-61 0.7087195471923202 1107 62-63 0.7626286548934225 1107 64-65 0.7353815430484367 1107 66-67 0.7260529203583133 1107 68-69 0.7565865510824494 1107 70-71 0.69716208367009 1107 72-73 0.5044695777570247 1107 74-75 0.8651878999158846 1107 76-77 0.8930829970171104 1107 78-79 0.8402079252504286 1107 80-81 0.8326388358221557 1107 82-83 0.8550902786924617 1107 84-85 0.8265864464254307 1107 86-87 0.9464702179052864 1107 88-89 1.1659832468502103 1107 90-91 1.426033744705098 1107 92-93 1.3148611472461802 1107 94-95 1.3555316402498079 1107 96-97 1.3615474678411985 1107 98-99 1.1057287988896825 1107 100-101 0.7236784595034127 1108 1 0.34327443818179404 1108 2 0.347213532646105 1108 3 0.22307973149731097 1108 4 0.23900214987145318 1108 5 0.3455803585600563 1108 6 0.22889024728518592 1108 7 0.2576642792096635 1108 8 0.18965721148639147 1108 9 0.22922182444393258 1108 10-11 0.23544951683972926 1108 12-13 0.3977391857599102 1108 14-15 0.40127145007068066 1108 16-17 0.4193172394334681 1108 18-19 0.4314915359221132 1108 20-21 0.3609957526127232 1108 22-23 0.4945430688576309 1108 24-25 0.4083563401867991 1108 26-27 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-0.9585219445397364 2316 58-59 -0.9808246250941757 2316 60-61 -1.2099748679763103 2316 62-63 -1.1457046822044816 2316 64-65 -1.2056478833980968 2316 66-67 -0.9823985263888915 2316 68-69 -0.8803435060501847 2316 70-71 -1.3191294359712593 2316 72-73 -1.1187423451511869 2316 74-75 -1.0080811691795049 2316 76-77 -1.1735284509227135 2316 78-79 -1.00002714660695 2316 80-81 -0.9579419591739153 2316 82-83 -0.9489752094827963 2316 84-85 -1.040797468887984 2316 86-87 -1.1147708436856512 2316 88-89 -1.3039310925963328 2316 90-91 -1.256746753853598 2316 92-93 -1.169618241953689 2316 94-95 -1.0606015867118082 2316 96-97 -1.189152268021445 2316 98-99 -1.5135477168282314 2316 100-101 -1.5098443522154863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6142.0 3 1710.0 4 1684.0 5 1915.0 6 2312.0 7 3037.0 8 3920.0 9 4944.0 10 6854.0 11 8980.0 12 12348.0 13 15766.0 14 18511.0 15 23725.0 16 28574.0 17 34875.0 18 29741.0 19 21909.0 20 22316.0 21 23296.0 22 25764.0 23 29043.0 24 32364.0 25 36055.0 26 41285.0 27 46990.0 28 54913.0 29 63064.0 30 73023.0 31 86087.0 32 102420.0 33 124181.0 34 154415.0 35 193660.0 36 227442.0 37 234224.0 38 119146.0 39 6796.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.726242975798044 18.975618877179787 14.859094079123153 21.43904406789901 2 15.870946801394439 21.219207966318752 39.82093052045936 23.08891471182745 3 18.698277790203534 26.80176642237052 30.06673497942815 24.433220807997795 4 12.670781993615297 17.25321432974391 36.662787848775 33.41321582786579 5 13.322176332974555 38.23611882049316 32.52315892586907 15.918545920663218 6 30.16804156480871 36.41680925479775 17.738865249924178 15.676283930469362 7 27.786769631481935 34.64936278038287 20.19188361536317 17.37198397277202 8 27.099264233597903 33.188333211113004 19.90267100834895 19.80973154694015 9 28.4818715129273 16.235618059987402 19.93293585840697 35.349574568678335 10-11 25.188540601212996 27.23522590413537 26.105477670431625 21.470755824220007 12-13 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26.010262572040865 30.840979547677005 21.318321785989223 38-39 21.26431222531994 26.281528239098996 30.91308058217741 21.54107895340365 40-41 21.292217174733622 26.22009263330436 31.056104434911102 21.431585757050918 42-43 21.567993235634496 26.22743878959043 30.460159379641954 21.74440859513312 44-45 21.08819026718108 26.687432426144163 30.360408628968543 21.86396867770621 46-47 21.50298394738997 26.950524059416363 29.590722789922562 21.955769203271096 48-49 21.666130240746913 26.831989842051595 29.395837092214478 22.106042824987014 50-51 21.81315297370076 27.250277327372647 28.777521183339715 22.159048515586875 52-53 22.004108650992922 27.303004951491193 28.536302182909324 22.15658421460656 54-55 21.474045333962827 27.40388865028665 28.648950363917518 22.473115651833 56-57 21.56554753451923 27.536011472092408 28.610012291527582 22.28842870186078 58-59 21.609947848151286 26.834907941327867 28.78064506065317 22.774499149867673 60-61 21.68475516956119 27.135224146474496 28.988383598625024 22.19163708533929 62-63 21.585138546245773 27.183867856595086 28.905511954309244 22.3254816428499 64-65 21.423719296056404 26.946731086942677 28.88448675003867 22.74506286696225 66-67 21.514835859160268 27.157191035391904 28.74875186099767 22.57922124445015 68-69 21.643479131218097 26.96398186939674 28.774650432142174 22.617888567242986 70-71 21.62322877158515 27.208684164203277 28.522260464083494 22.645826600128082 72-73 21.77428413048882 27.124078155176733 28.2689545647894 22.832683149545044 74-75 21.503510458313517 27.42281164791467 28.199680765787704 22.87399712798411 76-77 21.48355615247844 27.167322735872286 28.473611510836257 22.87550960081302 78-79 21.77992657598803 27.14177198209997 28.348729221789704 22.729572220122296 80-81 21.577063149545666 27.554059643360645 28.13615342880562 22.732723778288065 82-83 21.765675091949515 27.317715346964494 27.891821406325878 23.02478815476011 84-85 21.76398990710397 26.858297549098364 27.968037776089737 23.409674767707934 86-87 21.897979106033567 26.91544273917118 27.831636798026295 23.35494135676896 88-89 21.867615120903995 26.79572176271863 27.990017931734595 23.34664518464278 90-91 21.865246600271384 26.806379688848182 27.958337299054936 23.370036411825495 92-93 22.145489765274085 26.286338314731633 28.024363132885675 23.5438087871086 94-95 21.987089224768404 26.347706809686333 28.100087762067282 23.565116203477977 96-97 22.294803846282868 26.194916159989432 27.997084368423288 23.513195625304416 98-99 22.40479910432805 26.156855434314313 27.584813617916144 23.853531843441495 100-101 22.950334533555644 25.655285463602134 27.808762264885612 23.58561773795661 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2819.0 1 1882.5 2 1376.0 3 1749.5 4 1451.0 5 1401.0 6 2354.0 7 4750.5 8 7363.5 9 7803.5 10 6073.5 11 4643.0 12 4478.5 13 4600.0 14 4677.0 15 4896.5 16 5116.0 17 5270.5 18 5485.5 19 5746.5 20 6165.5 21 6638.5 22 7292.0 23 8245.0 24 9267.5 25 10682.0 26 12362.0 27 14396.5 28 16320.0 29 18391.5 30 21352.5 31 24832.0 32 29251.0 33 32342.5 34 34273.0 35 36674.0 36 39783.5 37 43062.0 38 45481.5 39 47214.0 40 49303.0 41 54846.0 42 59970.5 43 63367.0 44 66062.5 45 68159.0 46 68666.0 47 68878.0 48 71320.5 49 70483.0 50 68026.0 51 65167.5 52 63057.5 53 62887.0 54 61723.5 55 58046.0 56 51914.0 57 46242.0 58 42808.5 59 39029.5 60 33726.0 61 28922.0 62 24323.5 63 18816.5 64 14316.5 65 11591.5 66 9189.5 67 7543.5 68 5961.5 69 4401.0 70 3619.0 71 3065.0 72 2695.0 73 2422.0 74 1938.0 75 1566.0 76 1121.0 77 670.5 78 408.0 79 245.0 80 133.5 81 71.5 82 47.0 83 33.0 84 21.5 85 22.0 86 16.0 87 8.0 88 4.0 89 3.5 90 5.5 91 5.0 92 3.0 93 1.5 94 1.0 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17999086008284154 2 0.048715030588568034 3 0.04684337519775859 4 0.05323819778302419 5 0.06082880020130693 6 0.06384424499761104 7 0.036549270548306645 8 0.03504154815015459 9 0.04128039945285274 10-11 0.04338601176751336 12-13 0.03888883978681845 14-15 0.03472960558501968 16-17 0.03239003634650788 18-19 0.04083848081891162 20-21 0.050560690765616235 22-23 0.04829910716838815 24-25 0.06823743612326098 26-27 0.05323819778302419 28-29 0.04538764322712902 30-31 0.05469392975365376 32-33 0.050378724269287536 34-35 0.04996280084910767 36-37 0.04736327947298343 38-39 0.026619098891512094 40-41 0.024565477004373955 42-43 0.02144605135302488 44-45 0.003769305995380131 46-47 0.011619860551275299 48-49 0.010814008924676788 50-51 0.00844844447240374 52-53 0.008942353533867345 54-55 0.01003415251183952 56-57 0.008058516265985107 58-59 0.010450075932019397 60-61 0.010502066359541882 62-63 0.0034313682164839807 64-65 0.0064208177990268435 66-67 0.0035093538577677078 68-69 0.008500434899926225 70-71 0.02269382161356451 72-73 0.020016314596156555 74-75 0.020354252375052704 76-77 0.01219175525402263 78-79 0.004263215056843734 80-81 0.006004894378846967 82-83 0.004835109759591064 84-85 0.0050950618972034865 86-87 0.007720578487088957 88-89 0.030310419245608497 90-91 0.03660126097582913 92-93 0.023057754606221902 94-95 0.0321560794226567 96-97 0.03595138063179808 98-99 0.02108211836036749 100-101 0.12467304519891798 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1923431.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.98342960867101 #Duplication Level Percentage of deduplicated Percentage of total 1 69.36818326768707 27.04209689497269 2 13.77370869895146 10.738928070318273 3 5.837275132949146 6.826710127553061 4 3.2166171049478733 5.015790659551302 5 1.9020605458288227 3.7074421699874134 6 1.2692278010823401 2.9687311584517015 7 0.8624429331989764 2.3534688364500633 8 0.5998617027564852 1.8707733171475978 9 0.4603961251007822 1.6153037941474113 >10 2.3608498600581256 16.737728189666694 >50 0.20915571711568043 5.645542155679329 >100 0.1290738159361795 9.392496056055787 >500 0.008058285099005838 2.0312789450449427 >1k 0.002686095033001946 1.8359888333090446 >5k 0.0 0.0 >10k+ 4.0291425495029193E-4 2.2177207916646564 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18704 0.9724289563805512 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13513 0.7025466471113339 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10141 0.5272349255055159 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4060 0.21108113574128728 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2826 0.14692494817854135 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2654 0.13798259464467402 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2578 0.1340313221529652 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2548 0.13247160932729066 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 1938 0.10075744853857507 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.199042752248456E-5 0.0 10-11 0.0 0.0 0.0 5.199042752248456E-5 0.0 12-13 0.0 0.0 0.0 5.199042752248456E-5 0.0 14-15 0.0 0.0 0.0 1.0398085504496912E-4 0.0 16-17 0.0 0.0 0.0 2.0796171008993823E-4 0.0 18-19 0.0 0.0 0.0 3.639329926573919E-4 0.0 20-21 0.0 0.0 0.0 5.718947027473301E-4 0.0 22-23 0.0 0.0 0.0 0.0013257559018233563 0.0 24-25 0.0 0.0 0.0 0.003379377788961496 0.0 26-27 0.0 0.0 0.0 0.007018707715535416 0.0 28-29 0.0 0.0 0.0 0.01583108518059655 0.0 30-31 0.0 0.0 0.0 0.04039656218497051 0.0 32-33 0.0 0.0 0.0 0.08269077497451169 0.0 34-35 0.0 0.0 0.0 0.1434935799620574 0.0 36-37 0.0 0.0 0.0 0.2410796124217609 0.0 38-39 0.0 0.0 0.0 0.38712072333242004 0.0 40-41 0.0 0.0 0.0 0.5481610725833159 0.0 42-43 0.0 0.0 0.0 0.7069658334507452 0.0 44-45 0.0 0.0 0.0 0.9002142525518202 0.0 46-47 0.0 0.0 0.0 1.1263206218471056 0.0 48-49 0.0 0.0 0.0 1.3635269474184413 0.0 50-51 0.0 0.0 0.0 1.6301858501812645 0.0 52-53 0.0 0.0 0.0 1.8791420123726819 0.0 54-55 0.0 0.0 0.0 2.153521493622594 0.0 56-57 0.0 0.0 0.0 2.4646842023446642 0.0 58-59 0.0 0.0 0.0 2.795551283097756 0.0 60-61 0.0 0.0 0.0 3.159718232678999 0.0 62-63 0.0 0.0 0.0 3.5198559241272496 0.0 64-65 0.0 0.0 0.0 3.887480237138738 0.0 66-67 0.0 0.0 0.0 4.297008834733349 0.0 68-69 0.0 0.0 0.0 4.7445684300606565 0.0 70-71 0.0 0.0 0.0 5.230055042265618 0.0 72-73 0.0 0.0 0.0 5.711096472917406 0.0 74-75 0.0 0.0 0.0 6.206617237634207 0.0 76-77 0.0 0.0 0.0 6.731434608259927 0.0 78-79 0.0 0.0 0.0 7.296986478849514 0.0 80-81 0.0 0.0 0.0 7.897631888016779 0.0 82-83 0.0 0.0 0.0 8.517331788871033 5.199042752248456E-5 84-85 0.0 0.0 0.0 9.129934996368469 5.199042752248456E-5 86-87 0.0 0.0 0.0 9.763646317440033 5.199042752248456E-5 88-89 0.0 0.0 0.0 10.435414631458055 5.199042752248456E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6615 0.0 58.79949 1 GTATCAA 13200 0.0 47.752422 1 TATCAAC 17815 0.0 35.265213 2 ATCAACG 18100 0.0 34.683678 3 TCAACGC 18280 0.0 34.29016 4 CAACGCA 18530 0.0 33.800125 5 AACGCAG 18940 0.0 33.089226 6 ACGCAGA 21325 0.0 29.250257 7 CGCAGAG 21450 0.0 29.036263 8 GTACATG 14975 0.0 28.07212 1 TACATGG 15195 0.0 27.27198 2 GCAGAGT 22130 0.0 27.19806 9 TGGTATC 2585 0.0 27.0245 2 GTGGTAT 2625 0.0 26.297838 1 ACATGGG 15375 0.0 25.839973 3 CATGGGA 9285 0.0 24.976917 4 AGTACTT 13590 0.0 24.414948 12-13 GAGTACT 13415 0.0 24.414759 12-13 GTACTTT 14210 0.0 22.899303 14-15 AGAGTAC 21535 0.0 22.58904 10-11 >>END_MODULE