FastQCFastQC Report
Thu 26 May 2016
SRR938654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938654_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923431
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175340.9116001561792443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137350.7140885220213254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104400.5427800633347388No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46280.24061169857405854No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30140.15669914855276848No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG27570.14333760867948991No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27270.1417778958538154No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG22750.11827822261365238No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC22090.11484685439716841No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68100.059.0855331
ACCGAAC857.232302E-944.701178
GTATCAA124700.043.480681
TATCAAC172350.031.4870722
ATCAACG174900.030.9582983
TCAACGC178300.030.3679544
CAACGCA182150.029.7260845
AACGCAG187950.028.90896
AGGTAAG4150.026.3595312
GTGGTAT26750.026.3145451
TGGTATC26000.025.793112
GTACATG145750.025.3553961
ACGCAGA214000.025.2344917
CGCAGAG215750.024.985788
TACATGG147000.024.8162382
CGACCGT500.001655238623.74780718-19
ACATGGG148900.023.4338973
GCAGAGT226900.023.4021179
GAGTACT133350.022.43858112-13
AGTACTT135600.022.32895312-13
Produced by FastQC (version 0.11.5)