##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938654_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1923431 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.759175660577373 31.0 31.0 34.0 30.0 34.0 2 31.96373979622872 31.0 31.0 34.0 30.0 34.0 3 32.00173596037497 33.0 31.0 34.0 30.0 34.0 4 35.708806814489314 37.0 35.0 37.0 33.0 37.0 5 35.585923799709995 37.0 35.0 37.0 33.0 37.0 6 35.6299732093327 37.0 35.0 37.0 33.0 37.0 7 35.562130900458605 37.0 35.0 37.0 33.0 37.0 8 35.5675576612834 37.0 35.0 37.0 33.0 37.0 9 37.108479066834214 39.0 37.0 39.0 33.0 39.0 10-11 37.02898804272158 39.0 37.0 39.0 33.0 39.0 12-13 36.912470475935976 39.0 37.0 39.0 33.0 39.0 14-15 37.876434870811586 40.0 37.0 41.0 32.5 41.0 16-17 37.83371121709071 40.0 37.0 41.0 32.5 41.0 18-19 38.01032373919314 40.0 37.0 41.0 33.0 41.0 20-21 38.08411271316726 40.0 37.0 41.0 33.0 41.0 22-23 37.96871008109987 40.0 37.0 41.0 33.0 41.0 24-25 37.850478649870986 40.0 37.0 41.0 32.0 41.0 26-27 37.63753339735088 40.0 37.0 41.0 32.0 41.0 28-29 37.48524225719561 39.5 36.0 41.0 32.0 41.0 30-31 37.17527506835441 39.0 36.0 41.0 31.5 41.0 32-33 36.86279258262968 39.0 35.0 40.5 31.0 41.0 34-35 36.6263123033787 39.0 35.0 40.0 30.0 41.0 36-37 36.54260303592902 39.0 35.0 41.0 30.0 41.0 38-39 36.36595308071878 39.0 35.0 40.5 30.0 41.0 40-41 36.12183124843054 38.5 35.0 40.0 30.0 41.0 42-43 35.798153923899534 38.0 35.0 40.0 29.5 41.0 44-45 35.3733310422885 38.0 35.0 40.0 27.5 41.0 46-47 34.80433974496616 38.0 34.0 40.0 25.0 41.0 48-49 34.287449094872656 38.0 33.5 40.0 23.0 41.0 50-51 33.86251495374672 38.0 33.0 40.0 21.5 41.0 52-53 33.370664453260865 37.0 33.0 40.0 19.5 41.0 54-55 33.04878677737855 37.0 32.0 40.0 18.0 41.0 56-57 32.709283306757555 36.5 32.0 40.0 15.5 41.0 58-59 32.27917013919397 36.0 31.0 39.0 12.5 41.0 60-61 31.92524348416969 35.5 31.0 39.0 8.5 40.0 62-63 31.47799687121607 35.0 30.5 39.0 7.5 40.0 64-65 30.99560005011877 35.0 30.0 38.0 6.5 40.0 66-67 30.520508403992658 35.0 29.0 38.0 2.0 40.0 68-69 30.016385563090125 34.0 29.0 37.0 2.0 39.5 70-71 29.47012292096779 34.0 28.0 36.5 2.0 39.0 72-73 29.07144186612361 34.0 28.0 36.0 2.0 39.0 74-75 28.611363235801022 34.0 27.0 36.0 2.0 38.5 76-77 27.350300582656722 31.5 25.5 34.5 2.0 36.5 78-79 28.047592037354082 33.0 26.5 35.0 2.0 37.0 80-81 27.799151620203688 33.0 26.0 35.0 2.0 36.5 82-83 27.364682954574405 33.0 26.0 35.0 2.0 36.0 84-85 26.892271155034933 33.0 25.0 35.0 2.0 36.0 86-87 26.357212709995835 32.0 24.5 35.0 2.0 35.0 88-89 25.774549489947912 32.0 23.0 34.0 2.0 35.0 90-91 25.27936718291428 32.0 20.0 34.0 2.0 35.0 92-93 24.61175888295447 31.0 18.0 34.0 2.0 35.0 94-95 23.906479359020416 31.0 9.5 34.0 2.0 35.0 96-97 22.98929205154747 30.5 2.0 34.0 2.0 35.0 98-99 21.84084872293313 30.0 2.0 34.0 2.0 35.0 100-101 18.34444334109204 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31261613527615495 1101 2 0.3272534351174521 1101 3 0.43482988791878086 1101 4 0.22050647073451302 1101 5 0.14838880390558984 1101 6 0.1853202486800285 1101 7 0.29352944609814813 1101 8 0.2756046104143337 1101 9 0.27767424334334834 1101 10-11 0.3172816284626805 1101 12-13 0.27761007330249043 1101 14-15 0.4875440722499107 1101 16-17 0.5549967059990095 1101 18-19 0.3988877502110739 1101 20-21 0.5277076036713453 1101 22-23 0.4127631027658012 1101 24-25 0.5524172798516176 1101 26-27 0.6084634462901519 1101 28-29 0.5580672364582071 1101 30-31 0.49120811431987477 1101 32-33 0.5149498108298403 1101 34-35 0.5231695577761997 1101 36-37 0.42732774365780557 1101 38-39 0.5813451258072746 1101 40-41 0.7325552937770752 1101 42-43 0.7116370512013006 1101 44-45 0.583069415005653 1101 46-47 0.5401044812553693 1101 48-49 0.7591759109224299 1101 50-51 0.8334646761693065 1101 52-53 0.9279718057354387 1101 54-55 0.8860824326688643 1101 56-57 0.9063289257844218 1101 58-59 0.9521977410108278 1101 60-61 0.8811560032701102 1101 62-63 0.903898858771921 1101 64-65 0.6915464877037003 1101 66-67 0.6243334773350888 1101 68-69 0.6339954991020846 1101 70-71 0.6540797942752405 1101 72-73 0.5413129474134202 1101 74-75 0.8022957109020794 1101 76-77 0.721601358547673 1101 78-79 0.6146272561113371 1101 80-81 0.8731800588625234 1101 82-83 0.9509925579778553 1101 84-85 0.8531047463767187 1101 86-87 0.8772432912071793 1101 88-89 0.8366682981623832 1101 90-91 0.9755786672255624 1101 92-93 0.9255098841784424 1101 94-95 1.0818303442748167 1101 96-97 1.1644498328578017 1101 98-99 1.0172912535289598 1101 100-101 0.879800159796595 1102 1 0.33148903556801557 1102 2 0.34736583294948176 1102 3 0.381611966972109 1102 4 0.2672809553397286 1102 5 0.16309311859722442 1102 6 0.28854343000131166 1102 7 0.28963363356535865 1102 8 0.25139086223369134 1102 9 0.3636750975445793 1102 10-11 0.4444508362502688 1102 12-13 0.4032974927856614 1102 14-15 0.548043257414399 1102 16-17 0.5349684393237979 1102 18-19 0.5842623063016532 1102 20-21 0.4764173835786636 1102 22-23 0.5436940009521223 1102 24-25 0.6587780481868108 1102 26-27 0.5097890955283759 1102 28-29 0.4817031446810489 1102 30-31 0.4393831475368728 1102 32-33 0.5117152029994756 1102 34-35 0.601497058248242 1102 36-37 0.6776736742529081 1102 38-39 0.8248807873150596 1102 40-41 0.8736582236315442 1102 42-43 0.6183479653986765 1102 44-45 0.6055906115283705 1102 46-47 0.5636599858716096 1102 48-49 0.7789789569231829 1102 50-51 0.705859135984575 1102 52-53 0.460638732053269 1102 54-55 0.5092910312562893 1102 56-57 0.6790657628291612 1102 58-59 0.7620214383365393 1102 60-61 0.6552149013505222 1102 62-63 0.6809075728785707 1102 64-65 0.7075758245690302 1102 66-67 0.6252042260989015 1102 68-69 0.4458599430804888 1102 70-71 0.3856139633694582 1102 72-73 0.43544599238996895 1102 74-75 0.3786643314785749 1102 76-77 0.39635814460509877 1102 78-79 0.49105548265442067 1102 80-81 0.7422510767066903 1102 82-83 0.69512654355119 1102 84-85 0.48956182485523314 1102 86-87 0.42504076190758866 1102 88-89 0.6521517753605757 1102 90-91 0.41885667488828915 1102 92-93 0.45298610972379905 1102 94-95 0.3466520593798954 1102 96-97 0.4840912703962523 1102 98-99 0.18247380943002156 1102 100-101 -0.004848185902684321 1103 1 0.2672428563140592 1103 2 0.2399553817360065 1103 3 0.3031025633925779 1103 4 0.25421939220524337 1103 5 0.21781499602843724 1103 6 0.18344306037516844 1103 7 0.3114595042544437 1103 8 0.21567669336251072 1103 9 0.34067373851910077 1103 10-11 0.3637437307782392 1103 12-13 0.3387012516001917 1103 14-15 0.5139086203239387 1103 16-17 0.5428234037581561 1103 18-19 0.5524334977096288 1103 20-21 0.4643085123550037 1103 22-23 0.39168026151205027 1103 24-25 0.40448651791887613 1103 26-27 0.5155104815642417 1103 28-29 0.5565033640095649 1103 30-31 0.4543126345947215 1103 32-33 0.4638421073200263 1103 34-35 0.42535309184371783 1103 36-37 0.5393391773180767 1103 38-39 0.6191731093118378 1103 40-41 0.7320162957652414 1103 42-43 0.7430992414889204 1103 44-45 0.8268819343764306 1103 46-47 0.5533395385251794 1103 48-49 0.6065278167354862 1103 50-51 0.6066470043429675 1103 52-53 0.7715683198766072 1103 54-55 0.5708531842614875 1103 56-57 0.530173954212934 1103 58-59 0.6761265459036991 1103 60-61 0.5572767334110971 1103 62-63 0.6031748487620554 1103 64-65 0.5300994962802967 1103 66-67 0.5939831184767783 1103 68-69 0.46280424920866636 1103 70-71 0.3391537111023979 1103 72-73 0.1473855906759134 1103 74-75 0.3625518313886076 1103 76-77 0.34360675597158163 1103 78-79 0.4053941829497134 1103 80-81 0.40003691208859493 1103 82-83 0.41713042236825615 1103 84-85 0.2812380064451183 1103 86-87 0.3735579388249626 1103 88-89 0.2823844651252223 1103 90-91 0.5846586013412605 1103 92-93 0.4578274335206167 1103 94-95 0.4532882848354838 1103 96-97 0.3957801327342345 1103 98-99 0.36866618907251336 1103 100-101 -0.18165677923606438 1104 1 0.286587422421146 1104 2 0.34347965163145133 1104 3 0.32267971278023566 1104 4 0.17942209177745383 1104 5 0.10728666441966084 1104 6 0.1559976155171725 1104 7 0.14138058655432673 1104 8 0.14086271365562908 1104 9 0.19128670202545095 1104 10-11 0.3242597073654707 1104 12-13 0.23963312910014878 1104 14-15 0.3863393163749578 1104 16-17 0.4214722277145384 1104 18-19 0.42238361431014937 1104 20-21 0.3539723013312255 1104 22-23 0.4730941392899979 1104 24-25 0.40303758276730406 1104 26-27 0.3866655216408432 1104 28-29 0.4032873519016462 1104 30-31 0.39114743251290207 1104 32-33 0.4227542476730761 1104 34-35 0.510834706027282 1104 36-37 0.6027088550064477 1104 38-39 0.5986611481066078 1104 40-41 0.546235998935849 1104 42-43 0.49567263803877637 1104 44-45 0.3755312216461206 1104 46-47 0.39044522029070805 1104 48-49 0.40755065808156843 1104 50-51 0.48804837266160206 1104 52-53 0.37827061018872854 1104 54-55 0.5473307676192789 1104 56-57 0.5523434959960696 1104 58-59 0.5785939981438233 1104 60-61 0.6415246986934839 1104 62-63 0.5758140358016384 1104 64-65 0.7230903381205778 1104 66-67 0.43984943714366764 1104 68-69 0.32318509274776375 1104 70-71 0.27693397704423717 1104 72-73 0.28693185250970643 1104 74-75 0.32679192034476046 1104 76-77 0.29846119837401375 1104 78-79 0.3691780627353545 1104 80-81 0.4605108816025769 1104 82-83 0.28234285151993177 1104 84-85 0.40181776583107265 1104 86-87 0.4509799091420241 1104 88-89 0.19574935524275006 1104 90-91 0.047495159850008406 1104 92-93 0.19746666689152903 1104 94-95 0.39341662262177124 1104 96-97 0.39048693262921574 1104 98-99 0.41595904941443607 1104 100-101 0.27826584744151717 1105 1 0.30889104973632087 1105 2 0.3241623110273686 1105 3 0.13386230987632786 1105 4 0.16956699382015472 1105 5 0.12541107348758374 1105 6 0.1848346611866063 1105 7 0.22662403224720862 1105 8 0.21808047716552892 1105 9 0.19834138660515066 1105 10-11 0.31359588450217757 1105 12-13 0.37470733917513144 1105 14-15 0.4099647259046364 1105 16-17 0.3155942034249435 1105 18-19 0.3405093426682839 1105 20-21 0.3483291107011226 1105 22-23 0.3792811706671202 1105 24-25 0.3556115912840525 1105 26-27 0.2752593781460462 1105 28-29 0.18849356429272035 1105 30-31 0.23548672464040266 1105 32-33 0.317542758121121 1105 34-35 0.1640135358168564 1105 36-37 0.18305362951776516 1105 38-39 0.16452334091354004 1105 40-41 0.39444814080352586 1105 42-43 0.4316756284299501 1105 44-45 0.298619680055495 1105 46-47 0.00878071579413131 1105 48-49 0.08255919682105883 1105 50-51 0.05641003408775447 1105 52-53 0.14003640685679386 1105 54-55 0.19316144404869107 1105 56-57 0.16622389500806634 1105 58-59 0.09342839057402585 1105 60-61 0.027906838435633574 1105 62-63 0.1483213468682827 1105 64-65 0.040462458586432604 1105 66-67 0.2205483677856499 1105 68-69 0.04680669099457191 1105 70-71 0.131452765935812 1105 72-73 0.17560486642821616 1105 74-75 -9.682913280251171E-4 1105 76-77 -0.09463862059005024 1105 78-79 0.14305435728888583 1105 80-81 0.22158009303349502 1105 82-83 0.3405056665920867 1105 84-85 0.21766742538090256 1105 86-87 0.37308095211423975 1105 88-89 0.1906885579516917 1105 90-91 0.35400981496555417 1105 92-93 0.42260310348864394 1105 94-95 0.5223222713827376 1105 96-97 0.49552720762793356 1105 98-99 0.16515376932179038 1105 100-101 0.045518132378884246 1106 1 0.11849911839065896 1106 2 0.08434902926061838 1106 3 0.15045377526899983 1106 4 0.09980464669781242 1106 5 -0.04420508404328416 1106 6 0.002808818911269384 1106 7 0.055501321462465114 1106 8 0.06495720184460652 1106 9 -0.011924745858628683 1106 10-11 0.08574318631416844 1106 12-13 0.006971424142037108 1106 14-15 0.09686748665505007 1106 16-17 0.04661083196435101 1106 18-19 0.13949359884975365 1106 20-21 0.15137423567847463 1106 22-23 0.29830393327122806 1106 24-25 0.2239975655091868 1106 26-27 0.19320994331416586 1106 28-29 0.2583342724064508 1106 30-31 0.19290847950531287 1106 32-33 0.319757088071448 1106 34-35 0.2619597195101875 1106 36-37 -0.1979455152520586 1106 38-39 -0.06318906582212946 1106 40-41 0.19804558438149655 1106 42-43 0.2825154813135029 1106 44-45 0.2704034037192571 1106 46-47 -0.01022856534909522 1106 48-49 0.162135204876229 1106 50-51 0.15160642105623623 1106 52-53 0.04557223173261349 1106 54-55 0.07096000574534145 1106 56-57 0.30561714500912274 1106 58-59 0.538222229546399 1106 60-61 0.04183318752963672 1106 62-63 0.1651398906177306 1106 64-65 -0.17407645438652253 1106 66-67 0.23371849118314714 1106 68-69 -0.6545540939634868 1106 70-71 -0.3002594217261212 1106 72-73 -0.09593155668743236 1106 74-75 0.1856715809351357 1106 76-77 0.17178158811981703 1106 78-79 0.12105092794227446 1106 80-81 -0.6036138082906355 1106 82-83 -0.06389308131723226 1106 84-85 0.12197812249882034 1106 86-87 0.14296926663672238 1106 88-89 0.3757220298912962 1106 90-91 0.3096768734371764 1106 92-93 0.03452703434559723 1106 94-95 0.12379090802787474 1106 96-97 0.2801507588975056 1106 98-99 0.3656559537397541 1106 100-101 0.15533052942475933 1107 1 0.1706746129044916 1107 2 0.1944172497958654 1107 3 0.20937360371018343 1107 4 0.11503716436789801 1107 5 0.05741682189244557 1107 6 0.13535789085554484 1107 7 0.060463338299094005 1107 8 0.10231834675632712 1107 9 0.19486145721194958 1107 10-11 0.18263963607250844 1107 12-13 0.2226626706125998 1107 14-15 0.24188309599500002 1107 16-17 0.18348878079502384 1107 18-19 0.19114363561608627 1107 20-21 0.13838577324425927 1107 22-23 0.17241781210716312 1107 24-25 0.292650661339799 1107 26-27 0.0934828777915726 1107 28-29 0.13766517732947392 1107 30-31 0.22157314168675413 1107 32-33 0.23373732583584683 1107 34-35 0.29266210055602215 1107 36-37 0.34785697229840906 1107 38-39 0.27059370659552684 1107 40-41 0.14306865166259541 1107 42-43 0.263318740826314 1107 44-45 0.402010550782002 1107 46-47 0.474942823371876 1107 48-49 0.5143491666654043 1107 50-51 0.5624104917836874 1107 52-53 0.7125517394038212 1107 54-55 0.5735862477542142 1107 56-57 0.6196074575361123 1107 58-59 0.32258148772985606 1107 60-61 0.34371729897483405 1107 62-63 0.5003747372589125 1107 64-65 0.5298631703089427 1107 66-67 0.5355437241456755 1107 68-69 0.4796727530545155 1107 70-71 0.4015465775987366 1107 72-73 0.39244831681541825 1107 74-75 0.2840545161190846 1107 76-77 0.3671036272517654 1107 78-79 0.6028615328728897 1107 80-81 0.5373864193728295 1107 82-83 0.5587051345338985 1107 84-85 0.6311634274762774 1107 86-87 0.74624343363703 1107 88-89 0.5282517111886129 1107 90-91 0.6614038447541972 1107 92-93 0.5704576938107699 1107 94-95 0.5084789698267329 1107 96-97 0.5789156191723279 1107 98-99 0.2229396145682898 1107 100-101 -0.17246142280254873 1108 1 0.19494124687276582 1108 2 0.19081848046208805 1108 3 0.1856113132268291 1108 4 0.20837306686051704 1108 5 0.08217344514129366 1108 6 0.1332015974127856 1108 7 0.06803749236203771 1108 8 0.18172166924372135 1108 9 0.12377578791432597 1108 10-11 0.14141582093908767 1108 12-13 0.1554859674375919 1108 14-15 0.2748982525750492 1108 16-17 0.27212587098857455 1108 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-0.24912381476953982 2316 76-77 -0.31031213181449147 2316 78-79 -0.07458381572111605 2316 80-81 -0.40457281845514714 2316 82-83 -0.3291226598355941 2316 84-85 -0.32349434817728095 2316 86-87 -0.1651133433360812 2316 88-89 -0.11492738315499906 2316 90-91 -0.39134005313979614 2316 92-93 -0.5975763779657406 2316 94-95 -0.5295122897429962 2316 96-97 -0.3927920489424963 2316 98-99 -0.3804164929135254 2316 100-101 -0.13059946901732644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 12.0 4 49.0 5 142.0 6 251.0 7 685.0 8 1338.0 9 2223.0 10 3879.0 11 6095.0 12 8551.0 13 12037.0 14 15240.0 15 20018.0 16 25904.0 17 32408.0 18 28656.0 19 19397.0 20 17231.0 21 17598.0 22 19401.0 23 21212.0 24 24399.0 25 28639.0 26 34014.0 27 40440.0 28 48337.0 29 57102.0 30 68652.0 31 80976.0 32 98128.0 33 120557.0 34 154830.0 35 204996.0 36 260711.0 37 295398.0 38 148107.0 39 5812.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.324063401550255 19.853407180843735 15.41018278350628 21.412346634099727 2 16.141024340638907 21.920983300433548 38.85248672955337 23.085505629374175 3 18.68491404364522 26.868834543505365 30.532156816666994 23.91409459618242 4 12.970311906171835 17.9841647555852 36.26217940752748 32.783343930715475 5 13.320015174167283 38.41426829655401 32.70584647760015 15.559870051678551 6 30.167809502914324 36.823728015197844 17.343642688508194 15.664819793379642 7 27.574163045100136 34.8843290973266 20.006904328774986 17.53460352879828 8 27.181479346022808 33.64804872126944 19.666990913632983 19.503481019074766 9 28.02642777411823 16.33476844243438 19.92725499381054 35.71154878963686 10-11 25.208572597613326 27.38338417130638 26.07275748389207 21.33528574718823 12-13 26.49465980323703 25.201475904256508 27.158421591416587 21.145442701089877 14-15 23.350564694028535 25.92094543552641 26.85549936545683 23.872990504988223 16-17 21.254908546238465 29.37074425856711 27.158161639278976 22.216185555915445 18-19 21.233046571465263 26.46996435016385 31.5433202438767 20.753668834494192 20-21 22.684515327037985 25.563303284599236 31.309077372674142 20.443104015688633 22-23 22.0248607826327 25.653948594984694 31.427797513921735 20.89339310846087 24-25 21.58392847348209 26.095564348312767 31.506116818084806 20.81439036012034 26-27 21.028136634611112 26.456402111917924 31.608264130645868 20.907197122825096 28-29 20.859235397578598 26.378903116358217 31.896933136670874 20.864928349392308 30-31 21.057240940797982 26.173618913285683 31.56746979746089 21.201670348455444 32-33 20.91447522682124 26.3831403362013 31.208943809265836 21.493440627711628 34-35 21.496084445569867 26.606661616127475 31.022572998403202 20.874680939899452 36-37 21.74811235558994 26.046711368082732 30.912890468013117 21.29228580831421 38-39 21.159062898148573 26.304653507892844 31.058220906119537 21.47806268783904 40-41 21.258012120607113 26.209461061405808 31.082946505446785 21.449580312540295 42-43 21.586689584106693 26.243302315763646 30.511883482910186 21.65812461721948 44-45 21.14661248570913 26.688558102682137 30.35403921429986 21.810790197308872 46-47 21.503916698857406 26.993923878735448 29.520008775984163 21.98215064642298 48-49 21.598825224299702 26.846816964060576 29.37147212455243 22.182885687087296 50-51 21.73439026406458 27.226477061043518 28.800773201638112 22.238359473253784 52-53 22.001127152468687 27.374753760337644 28.42137305679278 22.20274603040088 54-55 21.442593989594634 27.38338417130638 28.585038922633565 22.58898291646542 56-57 21.553437581072572 27.555212534268193 28.412794636251576 22.478555248407663 58-59 21.547718634045097 26.991870256848312 28.59954425191234 22.860866857194253 60-61 21.610237123140887 27.392534486550336 28.74467553034135 22.252552859967423 62-63 21.46739342352286 27.4136166049107 28.574562851487784 22.54442712007865 64-65 21.291275850290443 27.17872385336412 28.53434825579914 22.995652040546293 66-67 21.34711911867884 27.32828142217772 28.423512053073924 22.90108740606952 68-69 21.426889152886922 27.16161098878817 28.452778001627316 22.958721856697593 70-71 21.387515691364985 27.336235959656456 28.213886594810678 23.062361754167878 72-73 21.42179781858564 27.241216347246144 27.99884685231755 23.338138981850662 74-75 21.115106286630507 27.490562437643977 27.809783662632036 23.584547613093477 76-77 20.848733331219055 27.254213954126765 28.46579887711075 23.43125383754343 78-79 21.00088331736361 27.20651273687489 28.37143625115744 23.42116769460407 80-81 20.680481417570835 27.662455554036256 28.123747337401472 23.533315690991436 82-83 20.82612179028928 27.30580044618417 27.942244573600895 23.925833189925658 84-85 20.702519869798973 26.877476007151124 28.00011022529374 24.41989389775616 86-87 20.69590489732998 26.95044439649682 27.92107185471523 24.43257885145797 88-89 20.4927815096844 26.89476109977574 27.925940579543244 24.686516810996615 90-91 20.31625806008841 26.888642099319632 28.175630639202314 24.619469201389645 92-93 20.39345092443576 26.425217835034694 28.100855950856197 25.08047528967335 94-95 20.03401217305136 26.41490795865093 28.34492461120218 25.20615525709553 96-97 20.19617548017059 26.3115755127166 28.278581347602277 25.21366765951053 98-99 20.096541049962553 26.310022639237552 27.92791317388385 25.665523136916047 100-101 19.950364738844286 25.986583350273545 28.273538276132598 25.78951363474957 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2994.0 1 1985.0 2 1287.0 3 1703.0 4 1580.0 5 1499.0 6 2386.5 7 4816.0 8 7094.5 9 7303.0 10 5822.0 11 4513.5 12 4305.0 13 4414.0 14 4647.5 15 4842.5 16 4963.0 17 5140.0 18 5375.5 19 5562.0 20 5973.0 21 6612.5 22 7240.5 23 8121.5 24 9089.0 25 10296.0 26 11977.0 27 14245.5 28 16121.0 29 18316.0 30 21747.5 31 25225.5 32 29558.5 33 32742.5 34 34378.5 35 36609.5 36 40099.5 37 43629.0 38 46102.5 39 47745.5 40 49642.0 41 55283.5 42 60689.5 43 64389.5 44 67488.0 45 69646.5 46 69847.0 47 70097.5 48 72479.5 49 71704.0 50 69045.5 51 65661.5 52 62928.5 53 62321.5 54 61810.0 55 58002.0 56 51068.0 57 45512.5 58 42301.5 59 38564.5 60 32920.0 61 27723.5 62 23701.0 63 18333.5 64 13479.5 65 10861.0 66 8503.0 67 6993.5 68 5542.5 69 3887.0 70 3194.5 71 2792.0 72 2432.5 73 2246.5 74 1891.5 75 1550.5 76 1051.5 77 542.0 78 301.5 79 200.5 80 104.0 81 46.5 82 30.0 83 21.0 84 11.5 85 8.5 86 4.5 87 3.0 88 1.5 89 1.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.1592342017987647E-4 2 0.048143135885820705 3 6.758755577922993E-4 4 0.0 5 0.09067130559921308 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.3395692385118051E-4 26-27 0.00808451147974635 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01060604721458685 36-37 1.0398085504496912E-4 38-39 0.003925277277947585 40-41 0.004939090614636033 42-43 2.599521376124228E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.599521376124228E-5 68-69 8.318468403597529E-4 70-71 2.599521376124228E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014557319706295679 82-83 5.199042752248456E-4 84-85 0.005069066683442245 86-87 0.0018716553908094441 88-89 1.299760688062114E-4 90-91 0.0033793777889614966 92-93 7.53861199076026E-4 94-95 1.0398085504496912E-4 96-97 0.0 98-99 2.599521376124228E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1923431.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.9596923932941 #Duplication Level Percentage of deduplicated Percentage of total 1 67.48099888763669 24.94076961279273 2 14.390632141241028 10.637466745706393 3 6.250692712494485 6.930710396964038 4 3.3899375446801367 5.011641955354261 5 2.0815029193623413 3.846585380768789 6 1.329274634733012 2.94777489635643 7 0.9449735402873879 2.4448151958176774 8 0.6454676087192651 1.9085027414479323 9 0.5092921876628265 1.6940954334893292 >10 2.5902379056678986 17.355620368536982 >50 0.22794460511782513 5.831056974744946 >100 0.146356059689207 10.088473733234105 >500 0.00958743537935067 2.368872918651784 >1k 0.0026788422383479814 1.8201535928108443 >5k 0.0 0.0 >10k+ 4.229750902654708E-4 2.173460053323814 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17534 0.9116001561792443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13735 0.7140885220213254 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10440 0.5427800633347388 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4628 0.24061169857405854 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3014 0.15669914855276848 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2757 0.14333760867948991 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2727 0.1417778958538154 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2275 0.11827822261365238 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2209 0.11484685439716841 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.199042752248456E-5 0.0 10-11 0.0 0.0 0.0 1.0398085504496912E-4 0.0 12-13 0.0 0.0 0.0 1.0398085504496912E-4 0.0 14-15 0.0 0.0 0.0 2.0796171008993823E-4 0.0 16-17 0.0 0.0 0.0 3.1194256513490735E-4 0.0 18-19 0.0 0.0 0.0 4.6791384770236103E-4 0.0 20-21 0.0 0.0 0.0 6.758755577922993E-4 0.0 22-23 0.0 0.0 0.0 0.0014557319706295676 0.0 24-25 0.0 0.0 0.0 0.00353534907152895 0.0 26-27 0.0 0.0 0.0 0.007070698143057901 0.0 28-29 0.0 0.0 0.0 0.016143027745731457 0.0 30-31 0.0 0.0 0.0 0.04096845688771783 0.0 32-33 0.0 0.0 0.0 0.08388655480752884 0.0 34-35 0.0 0.0 0.0 0.145599192276718 0.0 36-37 0.0 0.0 0.0 0.24456297106576738 0.0 38-39 0.0 0.0 0.0 0.39156590488559245 0.0 40-41 0.0 0.0 0.0 0.5537760387557443 0.0 42-43 0.0 0.0 0.0 0.7142704885176541 0.0 44-45 0.0 0.0 0.0 0.9088446635205526 0.0 46-47 0.0 0.0 0.0 1.135912855725004 0.0 48-49 0.0 0.0 0.0 1.3748348654045817 0.0 50-51 0.0 0.0 0.0 1.642897509710512 0.0 52-53 0.0 0.0 0.0 1.8918536719019294 0.0 54-55 0.0 0.0 0.0 2.166363129220648 0.0 56-57 0.0 0.0 0.0 2.4776818092252855 0.0 58-59 0.0 0.0 0.0 2.8091207846811246 0.0 60-61 0.0 0.0 0.0 3.175601308287118 0.0 62-63 0.0 0.0 0.0 3.53597295665922 0.0 64-65 0.0 0.0 0.0 3.9043251356560233 0.0 66-67 0.0 0.0 0.0 4.315439441290069 0.0 68-69 0.0 0.0 0.0 4.764948677649471 0.0 70-71 0.0 0.0 0.0 5.252982820803034 0.0 72-73 0.0 5.199042752248456E-5 0.0 5.7400291458336685 0.0 74-75 0.0 5.199042752248456E-5 0.0 6.243998355022873 0.0 76-77 0.0 5.199042752248456E-5 0.0 6.775080572165053 0.0 78-79 0.0 5.199042752248456E-5 0.0 7.348716954234386 0.0 80-81 0.0 5.199042752248456E-5 0.0 7.963217812336392 0.0 82-83 0.0 5.199042752248456E-5 0.0 8.595265439727237 0.0 84-85 0.0 5.199042752248456E-5 0.0 9.219462512562188 0.0 86-87 0.0 5.199042752248456E-5 0.0 9.867913119836377 0.0 88-89 0.0 5.199042752248456E-5 0.0 10.557826093059745 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6810 0.0 59.085533 1 ACCGAAC 85 7.232302E-9 44.70117 8 GTATCAA 12470 0.0 43.48068 1 TATCAAC 17235 0.0 31.487072 2 ATCAACG 17490 0.0 30.958298 3 TCAACGC 17830 0.0 30.367954 4 CAACGCA 18215 0.0 29.726084 5 AACGCAG 18795 0.0 28.9089 6 AGGTAAG 415 0.0 26.359531 2 GTGGTAT 2675 0.0 26.314545 1 TGGTATC 2600 0.0 25.79311 2 GTACATG 14575 0.0 25.355396 1 ACGCAGA 21400 0.0 25.234491 7 CGCAGAG 21575 0.0 24.98578 8 TACATGG 14700 0.0 24.816238 2 CGACCGT 50 0.0016552386 23.747807 18-19 ACATGGG 14890 0.0 23.433897 3 GCAGAGT 22690 0.0 23.402117 9 GAGTACT 13335 0.0 22.438581 12-13 AGTACTT 13560 0.0 22.328953 12-13 >>END_MODULE