FastQCFastQC Report
Thu 26 May 2016
SRR938653_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938653_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918108
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187410.9770565578163483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133760.6973538507737833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101580.5295843612559876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41640.2170889230429152No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28330.14769762703664235No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25550.13320417828401737No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG25210.1314315982207467No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG24880.12971115286521925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65350.058.3670351
GTATCAA125450.045.531231
TATCAAC170850.033.410112
ATCAACG171950.033.1963773
TCAACGC175100.032.54494
CAACGCA178550.031.8894375
TGGTATC26100.031.5032582
GTGGTAT27250.031.1441671
AACGCAG183400.031.0696096
GTACATG146650.028.025181
ACGCAGA205900.027.458827
TACATGG148400.027.4471232
CGCAGAG207700.027.1751048
ACATGGG152800.025.5991973
GCAGAGT217450.025.1257119
CATGGGA95800.024.3097484
AGTACTT130500.023.60571312-13
GAGTACT128700.023.27148812-13
ATGGGAT35850.023.0685
AGGTAAG6000.022.179752