##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938653_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1918108 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23129198147341 31.0 31.0 34.0 28.0 34.0 2 31.336902822990155 31.0 31.0 34.0 28.0 34.0 3 31.36573644445464 31.0 31.0 34.0 28.0 34.0 4 35.073929622315326 37.0 35.0 37.0 32.0 37.0 5 35.0953085019196 37.0 35.0 37.0 32.0 37.0 6 35.1324722069873 37.0 35.0 37.0 32.0 37.0 7 35.062509514584164 37.0 35.0 37.0 32.0 37.0 8 35.030150022835 37.0 35.0 37.0 32.0 37.0 9 36.51613464935238 39.0 37.0 39.0 32.0 39.0 10-11 36.3883251099521 39.0 35.0 39.0 32.0 39.0 12-13 36.22325150617171 39.0 35.0 39.0 32.0 39.0 14-15 37.16026782642062 39.0 36.0 41.0 31.5 41.0 16-17 37.04520835114602 39.0 36.0 41.0 31.0 41.0 18-19 37.02064795100172 39.0 36.0 41.0 31.0 41.0 20-21 37.050894423046046 39.0 36.0 41.0 31.0 41.0 22-23 37.10015051290125 39.0 36.0 41.0 31.0 41.0 24-25 37.00730876467853 39.0 36.0 41.0 31.0 41.0 26-27 36.814174436475945 39.0 36.0 41.0 30.0 41.0 28-29 36.621223883118155 39.0 35.5 41.0 30.0 41.0 30-31 36.30251190235378 38.5 35.0 40.0 30.0 41.0 32-33 35.93112301288562 38.0 35.0 40.0 29.5 41.0 34-35 35.63825446742311 38.0 35.0 40.0 28.5 41.0 36-37 35.3292713444707 38.0 34.5 40.0 27.0 41.0 38-39 34.99237321360424 38.0 34.0 40.0 25.5 41.0 40-41 34.79921255737425 38.0 34.0 40.0 25.0 41.0 42-43 34.48779005144653 38.0 33.5 40.0 24.0 41.0 44-45 34.03703597503373 38.0 33.0 40.0 23.0 41.0 46-47 33.47315662100361 38.0 33.0 40.0 19.0 41.0 48-49 32.9594772557124 37.0 32.0 40.0 14.5 41.0 50-51 32.13953802392775 36.5 30.5 39.5 10.5 40.5 52-53 32.15122897146563 36.5 31.0 39.0 8.5 40.0 54-55 32.24234480018852 37.0 31.0 40.0 7.5 41.0 56-57 31.94486963194982 36.0 31.0 39.5 4.5 41.0 58-59 31.553375513787543 36.0 30.5 39.0 2.0 41.0 60-61 31.11637248788911 35.0 30.0 39.0 2.0 40.5 62-63 30.56678169321018 35.0 29.0 39.0 2.0 40.0 64-65 30.113856988240496 35.0 28.0 38.0 2.0 40.0 66-67 29.597845376798386 34.5 28.0 38.0 2.0 40.0 68-69 29.024035664310873 34.0 26.5 37.0 2.0 40.0 70-71 28.443578776586094 34.0 26.0 36.5 2.0 39.0 72-73 27.849561912050834 34.0 25.5 36.0 2.0 39.0 74-75 27.18853578630609 33.0 24.5 35.5 2.0 38.5 76-77 26.504295117897428 32.5 22.5 35.0 2.0 37.5 78-79 25.76640992060927 32.0 20.0 35.0 2.0 37.0 80-81 25.069343853422225 31.5 17.5 35.0 2.0 36.0 82-83 24.37736378764908 31.0 10.0 35.0 2.0 36.0 84-85 23.660682036673638 31.0 4.5 34.0 2.0 35.0 86-87 22.939082158043238 30.0 2.0 34.0 2.0 35.0 88-89 22.187131016605946 29.5 2.0 34.0 2.0 35.0 90-91 21.381419867911504 29.0 2.0 34.0 2.0 35.0 92-93 20.52843479095025 28.5 2.0 34.0 2.0 35.0 94-95 19.633606397554257 26.5 2.0 34.0 2.0 35.0 96-97 18.568263622277787 25.0 2.0 33.0 2.0 35.0 98-99 17.340128918705307 21.5 2.0 33.0 2.0 35.0 100-101 14.575938372604671 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.643563684878071 1101 2 0.676870916408884 1101 3 0.7673782507507845 1101 4 0.5211397630236618 1101 5 0.6045116461424769 1101 6 0.5508916157252557 1101 7 0.5876870816145612 1101 8 0.5531066521476262 1101 9 0.7674901244225438 1101 10-11 0.7683230407898449 1101 12-13 0.7930071929536808 1101 14-15 0.9821388367375192 1101 16-17 1.0840762810251263 1101 18-19 1.0941055121078804 1101 20-21 1.1428931780535692 1101 22-23 1.0287107611217934 1101 24-25 1.2002637582953284 1101 26-27 1.264494716292127 1101 28-29 1.3367980580287764 1101 30-31 1.3212231053918373 1101 32-33 1.303357404007528 1101 34-35 1.4274490448868562 1101 36-37 1.3862741463349337 1101 38-39 1.627225889212653 1101 40-41 1.6611551184868034 1101 42-43 1.6913661030776481 1101 44-45 1.7771468045616317 1101 46-47 1.838211529724326 1101 48-49 1.8904161577523837 1101 50-51 1.7795847611012832 1101 52-53 1.8518612868104114 1101 54-55 1.9975341939940563 1101 56-57 2.1150111390080255 1101 58-59 2.2002278765546315 1101 60-61 2.187505833773219 1101 62-63 2.161673120382904 1101 64-65 2.2573065694256336 1101 66-67 2.221621933728912 1101 68-69 2.4071059262295655 1101 70-71 2.5343668858572315 1101 72-73 2.764417314526849 1101 74-75 2.837420286445642 1101 76-77 2.9936222212995354 1101 78-79 3.2362350982147596 1101 80-81 3.5855756562846217 1101 82-83 3.5002279043784448 1101 84-85 3.4457867071811243 1101 86-87 3.68959893230495 1101 88-89 3.9981309739920903 1101 90-91 4.056028688214482 1101 92-93 4.309594360966894 1101 94-95 4.536944302375183 1101 96-97 4.635209458212909 1101 98-99 5.128525700571089 1101 100-101 5.104511426456705 1102 1 0.42643374605592044 1102 2 0.351816036693382 1102 3 0.391581825611226 1102 4 0.3818587084313734 1102 5 0.4019822478045043 1102 6 0.5004515917162564 1102 7 0.48630966415957033 1102 8 0.49523003921940756 1102 9 0.531568638344659 1102 10-11 0.565456896572428 1102 12-13 0.5716837816474722 1102 14-15 0.8640467064464517 1102 16-17 0.8239781315775829 1102 18-19 0.9413715300808079 1102 20-21 0.8057084017852816 1102 22-23 0.6795274755528382 1102 24-25 0.5503019416147126 1102 26-27 0.7760987115917786 1102 28-29 0.7184857748353579 1102 30-31 0.7558243080959457 1102 32-33 0.7115934473702552 1102 34-35 0.6298308527190883 1102 36-37 0.702876915823218 1102 38-39 0.6485669831999843 1102 40-41 0.9360179544225176 1102 42-43 0.9981954926799474 1102 44-45 0.994607148859636 1102 46-47 1.29393332126876 1102 48-49 1.2206557263795048 1102 50-51 1.2806453697236648 1102 52-53 1.2009540455059167 1102 54-55 1.30815444045259 1102 56-57 1.1188150289160674 1102 58-59 1.1066421106197843 1102 60-61 1.0813614895541157 1102 62-63 1.2946032040480517 1102 64-65 1.3725243834235528 1102 66-67 1.5330840448860457 1102 68-69 1.7290265331048182 1102 70-71 1.7103800119016377 1102 72-73 1.8137224062936212 1102 74-75 1.801151064455432 1102 76-77 1.9714237638492769 1102 78-79 2.2419513242938223 1102 80-81 2.7231870712654462 1102 82-83 2.9556760018346786 1102 84-85 3.1408210439889963 1102 86-87 3.2836838289000134 1102 88-89 3.5167182193544413 1102 90-91 3.9313450039045854 1102 92-93 3.794689128865855 1102 94-95 4.0769885302126525 1102 96-97 4.478385211382406 1102 98-99 4.888102880190495 1102 100-101 4.898650498277979 1103 1 0.5501724440159492 1103 2 0.577272598439734 1103 3 0.4865844277337885 1103 4 0.5653129781937878 1103 5 0.369170374983824 1103 6 0.509554061120653 1103 7 0.42602440275371123 1103 8 0.2949525676493323 1103 9 0.6398678553272958 1103 10-11 0.5856403743175065 1103 12-13 0.6524203377332967 1103 14-15 0.8244504685800464 1103 16-17 0.8551893849145529 1103 18-19 0.946452447165349 1103 20-21 0.8956846421248628 1103 22-23 0.8140100962175936 1103 24-25 1.025305694358181 1103 26-27 0.9307158755493035 1103 28-29 0.986164833601002 1103 30-31 0.9392731223282098 1103 32-33 1.2045728413369048 1103 34-35 1.0715280979078017 1103 36-37 1.2794601602699842 1103 38-39 1.2895228383725197 1103 40-41 1.2480498120735177 1103 42-43 1.443515705849002 1103 44-45 1.231677046434534 1103 46-47 1.3436502288494339 1103 48-49 1.1500305821385908 1103 50-51 1.2140705433252563 1103 52-53 1.3698607308117658 1103 54-55 1.3925265287700128 1103 56-57 1.4470841974998834 1103 58-59 1.4604591962821907 1103 60-61 1.6542014137189405 1103 62-63 1.7695122289497327 1103 64-65 1.7905247894472502 1103 66-67 1.7142868338853177 1103 68-69 1.7656342976798456 1103 70-71 1.8653874942439046 1103 72-73 2.1494430024739586 1103 74-75 2.133448067484739 1103 76-77 2.1894460911850544 1103 78-79 2.30934183510999 1103 80-81 2.574227953355237 1103 82-83 2.773810613900192 1103 84-85 2.8696401961400966 1103 86-87 2.904947699998729 1103 88-89 3.3862399578235944 1103 90-91 3.5503504508381454 1103 92-93 3.643936960044641 1103 94-95 3.675850341303182 1103 96-97 3.7991966702440685 1103 98-99 3.9693469658998524 1103 100-101 4.2900702388328025 1104 1 0.5615125856693304 1104 2 0.44151162674809896 1104 3 0.399866973429031 1104 4 0.48223168243966796 1104 5 0.3327387560844741 1104 6 0.32026774477795783 1104 7 0.43397877073022784 1104 8 0.4720155778704864 1104 9 0.5120535045554462 1104 10-11 0.6786597493965303 1104 12-13 0.6472524311243788 1104 14-15 0.6971243373238281 1104 16-17 0.758219785604723 1104 18-19 0.8884000324387529 1104 20-21 0.7941387014586354 1104 22-23 0.9388903267204185 1104 24-25 0.945116994648977 1104 26-27 0.8677436149705429 1104 28-29 0.8343623414038319 1104 30-31 0.8377640576520591 1104 32-33 0.8828473362907729 1104 34-35 1.0405477729528627 1104 36-37 0.9298892542486001 1104 38-39 0.9176789892876656 1104 40-41 1.1519048094829074 1104 42-43 1.203563173615045 1104 44-45 0.9902102744905363 1104 46-47 0.8934638036760916 1104 48-49 0.8473840636039185 1104 50-51 1.0342680189003275 1104 52-53 1.0166719492936451 1104 54-55 1.2872141240588135 1104 56-57 1.221550797149348 1104 58-59 1.2511983336691195 1104 60-61 1.2914295230156547 1104 62-63 1.355433750645215 1104 64-65 1.5595044586913112 1104 66-67 1.6186655323302048 1104 68-69 2.005909262415244 1104 70-71 2.061041581343133 1104 72-73 2.0463516789296 1104 74-75 1.9873446201064695 1104 76-77 2.1716038448112975 1104 78-79 2.3400815364826464 1104 80-81 2.594252855494414 1104 82-83 2.7884592136041952 1104 84-85 2.8215862877809634 1104 86-87 3.1089693830808933 1104 88-89 3.4376725125673993 1104 90-91 3.719504955658678 1104 92-93 3.7126364647288206 1104 94-95 3.87145422747475 1104 96-97 4.20586570854304 1104 98-99 4.312113631988069 1104 100-101 4.217307093007166 1105 1 0.529165330144135 1105 2 0.5485280211048718 1105 3 0.5007168962900508 1105 4 0.524198075867055 1105 5 0.47230222380692766 1105 6 0.4807818807284434 1105 7 0.5121702798660834 1105 8 0.4837444204140269 1105 9 0.5609136134793999 1105 10-11 0.5852530410933952 1105 12-13 0.622386727300885 1105 14-15 0.8027152869697431 1105 16-17 0.8115885749874252 1105 18-19 0.8281688943135137 1105 20-21 0.8154146545432681 1105 22-23 0.8757002581317792 1105 24-25 0.7908634304563336 1105 26-27 1.0184504124946585 1105 28-29 0.9873194130966212 1105 30-31 0.8938466521240755 1105 32-33 1.0185852217533338 1105 34-35 1.0990560630697814 1105 36-37 1.1487711107886796 1105 38-39 0.978903407957155 1105 40-41 1.1391455109829351 1105 42-43 1.1324803003274369 1105 44-45 1.1733026646836606 1105 46-47 1.1870508127282946 1105 48-49 1.1423000936416443 1105 50-51 1.1919788964259368 1105 52-53 1.278697033403013 1105 54-55 1.3618623517210366 1105 56-57 1.4604771712455218 1105 58-59 1.519948682188602 1105 60-61 1.7121715442428176 1105 62-63 1.8970341986089316 1105 64-65 1.9344591511577143 1105 66-67 1.872911661440412 1105 68-69 1.8082324933303155 1105 70-71 2.2225176310838357 1105 72-73 2.441975900569407 1105 74-75 2.3661574233184197 1105 76-77 2.41793277285829 1105 78-79 2.685186696429856 1105 80-81 3.1212224553011048 1105 82-83 3.1505500517238616 1105 84-85 3.4537018940189945 1105 86-87 3.6887489793559176 1105 88-89 3.9499473689773694 1105 90-91 4.160020810971972 1105 92-93 4.342604029181693 1105 94-95 4.746928213980077 1105 96-97 4.869362009589526 1105 98-99 5.040282371183963 1105 100-101 4.880579179848928 1106 1 0.12784995698518387 1106 2 0.2696520744497768 1106 3 0.20668797062351985 1106 4 0.23659685637414896 1106 5 0.231401243195414 1106 6 0.04602480751140803 1106 7 0.17903639102615898 1106 8 0.3329564734154147 1106 9 0.19240685948655312 1106 10-11 0.16294232126262642 1106 12-13 -0.04629613674463684 1106 14-15 0.3473902884952338 1106 16-17 0.19211807469249464 1106 18-19 0.3675436443016409 1106 20-21 0.32544761655954346 1106 22-23 0.27960586721153646 1106 24-25 0.4497554343706369 1106 26-27 0.45658095089309114 1106 28-29 0.2579418607156043 1106 30-31 0.6094262333019387 1106 32-33 0.5844663144153586 1106 34-35 0.578128233726467 1106 36-37 0.6605065547306523 1106 38-39 0.6123338927937283 1106 40-41 0.7240508115734698 1106 42-43 0.4216742507748066 1106 44-45 0.49064413738631885 1106 46-47 0.8723817832376355 1106 48-49 0.9185122133128587 1106 50-51 0.10856844078849548 1106 52-53 0.7537252268313992 1106 54-55 0.700840161967399 1106 56-57 0.7542384288419228 1106 58-59 -0.03978932232686461 1106 60-61 1.2099958760190113 1106 62-63 1.5668631622433615 1106 64-65 1.462149872073283 1106 66-67 1.5141735913851093 1106 68-69 0.9028424917709188 1106 70-71 1.140313729881747 1106 72-73 1.6479838410592365 1106 74-75 1.935528088366933 1106 76-77 1.897099439524954 1106 78-79 2.2297694619396324 1106 80-81 2.6209691946943856 1106 82-83 2.8381695489203373 1106 84-85 2.8316328240744326 1106 86-87 2.9938439669146497 1106 88-89 3.043610712322476 1106 90-91 3.3399362702367057 1106 92-93 3.5747906131777683 1106 94-95 3.6761799411806884 1106 96-97 4.012118643237677 1106 98-99 4.165668830202357 1106 100-101 3.9394198253936867 1107 1 0.433194318033884 1107 2 0.29064982335325595 1107 3 0.23837428109933256 1107 4 0.29993710477064184 1107 5 0.360909924123483 1107 6 0.41388833123995283 1107 7 0.3347074473399587 1107 8 0.36907006523334474 1107 9 0.5039916325067892 1107 10-11 0.33491951386113783 1107 12-13 0.4385046810770703 1107 14-15 0.5678678559197721 1107 16-17 0.6590678362325093 1107 18-19 0.6030174634658749 1107 20-21 0.6626270413126178 1107 22-23 0.5738344024684565 1107 24-25 0.5879276531534643 1107 26-27 0.7453888221862357 1107 28-29 0.7485370983649346 1107 30-31 0.684622595589893 1107 32-33 0.7416121947803092 1107 34-35 0.7077948980744395 1107 36-37 0.7892174260767675 1107 38-39 0.8930296298365406 1107 40-41 0.7876275669016977 1107 42-43 0.859252473302142 1107 44-45 0.9144836883013951 1107 46-47 0.7661549891425992 1107 48-49 0.8779966228508371 1107 50-51 1.039779302770647 1107 52-53 1.0808684906377621 1107 54-55 1.1384325642089763 1107 56-57 1.1219804886032705 1107 58-59 1.1842803947798082 1107 60-61 1.2565593821433119 1107 62-63 1.2901842974552338 1107 64-65 1.4884818689462556 1107 66-67 1.6859259151192205 1107 68-69 1.9032063485889807 1107 70-71 1.8453915925653916 1107 72-73 2.1637733541706545 1107 74-75 2.1607749892967476 1107 76-77 2.445051487477002 1107 78-79 2.4232758751835917 1107 80-81 2.592666886397435 1107 82-83 2.6316504386786796 1107 84-85 3.106079052725267 1107 86-87 3.396043267346407 1107 88-89 3.3276875085845994 1107 90-91 3.4864852220882803 1107 92-93 3.8016589510530707 1107 94-95 4.0091612341244875 1107 96-97 4.045731789598548 1107 98-99 4.440791672009723 1107 100-101 4.394260646062039 1108 1 0.4018993127447672 1108 2 0.44402931922539324 1108 3 0.4150714798770032 1108 4 0.36945051579533583 1108 5 0.3980107036187661 1108 6 0.3823281348122407 1108 7 0.4707411878879313 1108 8 0.3494003335910918 1108 9 0.3029747887028975 1108 10-11 0.43573160657705046 1108 12-13 0.5442453584566067 1108 14-15 0.44474147297984956 1108 16-17 0.6206864433368864 1108 18-19 0.7793837860539838 1108 20-21 0.6441957669932137 1108 22-23 0.7071775068616191 1108 24-25 0.5782734212224909 1108 26-27 0.5393965808652368 1108 28-29 0.6866223203068813 1108 30-31 0.6248766496810347 1108 32-33 0.7335018066404828 1108 34-35 0.7277278601074926 1108 36-37 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3.8383262362109534 2310 100-101 3.490001325136051 2311 1 -0.19813731329774953 2311 2 -0.13795243858233874 2311 3 -0.41790770851599035 2311 4 -0.18268521118501013 2311 5 -0.10786204464848481 2311 6 -0.042285124933499674 2311 7 -0.15040620444035824 2311 8 -0.16207163257502089 2311 9 -0.23334510482995086 2311 10-11 -0.271504925896636 2311 12-13 -0.1983800391409858 2311 14-15 -0.12431958626766715 2311 16-17 -0.052563125398449984 2311 18-19 -0.1159179221361768 2311 20-21 -0.06276793408235903 2311 22-23 -0.20391254194515085 2311 24-25 -0.21573165050583754 2311 26-27 -0.0530503929196513 2311 28-29 -2.3573374122776158E-4 2311 30-31 0.061834889468848075 2311 32-33 0.13159566809711265 2311 34-35 -0.14264786903500237 2311 36-37 0.09174822860757814 2311 38-39 0.14778721792746552 2311 40-41 -0.05459194198447648 2311 42-43 0.22428250499883973 2311 44-45 0.3161036732547089 2311 46-47 0.1441812838355574 2311 48-49 0.2805015253557386 2311 50-51 0.31632374598175517 2311 52-53 0.44127351770945467 2311 54-55 0.270325362768439 2311 56-57 0.42015762011373425 2311 58-59 0.637592514758591 2311 60-61 0.4526996616169221 2311 62-63 0.6317193056569117 2311 64-65 0.6194527499171336 2311 66-67 0.6017477476077246 2311 68-69 0.4884852672012343 2311 70-71 0.8355310827048825 2311 72-73 0.8469512873485918 2311 74-75 0.8770432888983812 2311 76-77 1.142825935251448 2311 78-79 1.4493979013056197 2311 80-81 1.610895571292783 2311 82-83 1.5720063790346188 2311 84-85 1.9744082543878 2311 86-87 2.263296384599524 2311 88-89 2.1622048431019323 2311 90-91 2.5541336230700153 2311 92-93 2.8967613794888294 2311 94-95 2.977939369569288 2311 96-97 2.859781936982028 2311 98-99 3.1334354479868303 2311 100-101 3.2416632434646377 2312 1 -0.25094684201089024 2312 2 -0.37730872412336325 2312 3 -0.33076140249418273 2312 4 -0.2622055718298668 2312 5 -0.23702828224845263 2312 6 -0.19178912383509328 2312 7 -0.25471517822724365 2312 8 -0.10608128943608364 2312 9 -0.03134050523498644 2312 10-11 -0.45177872765637517 2312 12-13 -0.3289507550530715 2312 14-15 -0.3875863284500696 2312 16-17 -0.33620195421800503 2312 18-19 -0.10116088830283587 2312 20-21 -0.30946412053758365 2312 22-23 -0.3624040578663781 2312 24-25 -0.4638609197194441 2312 26-27 -0.23446265164114521 2312 28-29 -0.0893328629990009 2312 30-31 0.02816459575839758 2312 32-33 -0.011071868144718167 2312 34-35 -0.21495704877389699 2312 36-37 -0.2846033314789267 2312 38-39 -0.10545080539088048 2312 40-41 0.1210050328092862 2312 42-43 0.16816344185166798 2312 44-45 -0.030619401380015177 2312 46-47 -0.4176100410079684 2312 48-49 -0.12408262320430907 2312 50-51 -0.13023742748704592 2312 52-53 -0.14016048039121642 2312 54-55 0.136416507976044 2312 56-57 0.15624441744551376 2312 58-59 0.4449444295402252 2312 60-61 0.32947742795292356 2312 62-63 0.4993636722972603 2312 64-65 0.451172792480552 2312 66-67 0.5611952241234732 2312 68-69 0.571137814713218 2312 70-71 0.605053617287659 2312 72-73 0.7494587531090673 2312 74-75 0.7117210312066433 2312 76-77 0.9904729335008575 2312 78-79 1.1459447725512852 2312 80-81 1.119119349596886 2312 82-83 1.2931816785231725 2312 84-85 1.3813579906548092 2312 86-87 1.7836255645672559 2312 88-89 1.7429981016060339 2312 90-91 1.8248546839517203 2312 92-93 2.0215053696946654 2312 94-95 2.0517331200698656 2312 96-97 2.1245475521492487 2312 98-99 2.294967616547112 2312 100-101 2.232627098886324 2313 1 -0.34089718679156533 2313 2 -0.44777322149180776 2313 3 -0.4323522400859048 2313 4 -0.2899079125973145 2313 5 -0.4055932974256109 2313 6 -0.3445839304433136 2313 7 -0.239602646236186 2313 8 -0.2981759914622515 2313 9 -0.4030123726623529 2313 10-11 -0.40784299885550723 2313 12-13 -0.33495465774924327 2313 14-15 -0.2638449267104406 2313 16-17 -0.3276853188692641 2313 18-19 -0.3245137209460438 2313 20-21 -0.24485515538976443 2313 22-23 -0.4958129226834487 2313 24-25 -0.31464889873500823 2313 26-27 -0.2655511552675094 2313 28-29 -0.1762775365414342 2313 30-31 -0.16616331574648058 2313 32-33 -0.17384597090721599 2313 34-35 -0.10806951274410181 2313 36-37 -0.10095936960461671 2313 38-39 -0.08996054512611096 2313 40-41 0.0699796185198025 2313 42-43 0.12274081981801288 2313 44-45 0.23747010555386083 2313 46-47 0.05095986316765533 2313 48-49 0.005185259901857364 2313 50-51 0.23193554102780212 2313 52-53 0.32374213075910774 2313 54-55 0.15373950497463085 2313 56-57 0.2782572393754279 2313 58-59 0.1305586578011919 2313 60-61 0.37046955453972075 2313 62-63 0.5000226242660375 2313 64-65 0.44518525730102 2313 66-67 0.586213929856779 2313 68-69 0.7236647892309094 2313 70-71 0.9185285473537235 2313 72-73 1.0946067409673468 2313 74-75 1.211194294761924 2313 76-77 1.1911361367726592 2313 78-79 1.140002726314112 2313 80-81 1.4537878755000726 2313 82-83 1.5883607008935279 2313 84-85 1.5300770730535653 2313 86-87 1.5639086588356506 2313 88-89 1.4295040406763597 2313 90-91 1.6010724697030518 2313 92-93 1.7582347147389221 2313 94-95 1.708834496641785 2313 96-97 1.7953496029778329 2313 98-99 1.7385200903803408 2313 100-101 1.7530252759275857 2314 1 -0.3469773066125015 2314 2 -0.42063661644614214 2314 3 -0.5580173672704873 2314 4 -0.43422663962502384 2314 5 -0.3394785914082803 2314 6 -0.3124390021815131 2314 7 -0.42326786520560944 2314 8 -0.3137671066897738 2314 9 -0.3782963841604925 2314 10-11 -0.34697953297651907 2314 12-13 -0.5119601564955474 2314 14-15 -0.5595135166866996 2314 16-17 -0.5011071411321026 2314 18-19 -0.40946789110679305 2314 20-21 -0.47315940961708236 2314 22-23 -0.4328303743191029 2314 24-25 -0.4227974107775978 2314 26-27 -0.47328381940591413 2314 28-29 -0.4544392113644449 2314 30-31 -0.479608125939599 2314 32-33 -0.4175220997564466 2314 34-35 -0.5221605716491098 2314 36-37 -0.3710145656695474 2314 38-39 -0.30768075573060116 2314 40-41 -0.2089517973447883 2314 42-43 -0.2688197727782864 2314 44-45 -0.47532897743214875 2314 46-47 -0.36991732871688754 2314 48-49 -0.18670481940858252 2314 50-51 -0.1353426421330184 2314 52-53 -0.22754285699596366 2314 54-55 -0.2779282464219577 2314 56-57 -0.3781099166713098 2314 58-59 -0.05825537776960843 2314 60-61 0.01879646293427939 2314 62-63 0.174802862155655 2314 64-65 0.0318361953838 2314 66-67 0.4301474734608348 2314 68-69 0.549792595448082 2314 70-71 0.6678898083105409 2314 72-73 0.47909468586306403 2314 74-75 0.4814597615504148 2314 76-77 0.7465764745408698 2314 78-79 0.8365562055853424 2314 80-81 1.0072642263017322 2314 82-83 1.2735364062808792 2314 84-85 1.282873381634193 2314 86-87 1.6517723041122814 2314 88-89 1.6383410433172578 2314 90-91 1.7886160153514084 2314 92-93 2.0613761551768235 2314 94-95 2.1360528736354993 2314 96-97 2.193748166127886 2314 98-99 2.0401765981340745 2314 100-101 2.1529253556135135 2315 1 -0.6419654428401635 2315 2 -0.6599265843660085 2315 3 -0.5807098094133281 2315 4 -0.37798338743556315 2315 5 -0.41144946252161674 2315 6 -0.39317761868313994 2315 7 -0.48263869857761676 2315 8 -0.530736477430338 2315 9 -0.6163848164410908 2315 10-11 -0.5358303711744625 2315 12-13 -0.5849218923495059 2315 14-15 -0.435616754707695 2315 16-17 -0.43375679944926304 2315 18-19 -0.5252740862542495 2315 20-21 -0.5362867433154577 2315 22-23 -0.40705613556493603 2315 24-25 -0.6437565356735817 2315 26-27 -0.567534762165927 2315 28-29 -0.6885567601574891 2315 30-31 -0.4847366166264706 2315 32-33 -0.38799074196548844 2315 34-35 -0.6564083589859635 2315 36-37 -0.49530915844980683 2315 38-39 -0.33843097305263825 2315 40-41 -0.3827893178008992 2315 42-43 -0.25594502760964133 2315 44-45 -0.3677364724901935 2315 46-47 -0.34094188223998145 2315 48-49 -0.3789932127104336 2315 50-51 -0.3370969955342247 2315 52-53 -0.27059829654331224 2315 54-55 -0.11859453567526401 2315 56-57 -0.12833744742895448 2315 58-59 -0.0792766830629823 2315 60-61 0.004597058752416672 2315 62-63 0.01748398189777589 2315 64-65 0.13049699667567083 2315 66-67 0.21508960856862913 2315 68-69 0.24550214802764003 2315 70-71 0.29658405090070517 2315 72-73 0.4214410832669593 2315 74-75 0.48235658230691314 2315 76-77 0.4283919565317582 2315 78-79 0.6572966163471925 2315 80-81 0.8017855455160934 2315 82-83 0.9429145713713822 2315 84-85 1.0518332984795116 2315 86-87 1.3160581730755965 2315 88-89 1.5858428453113618 2315 90-91 1.725674604134806 2315 92-93 1.7802827082482544 2315 94-95 1.7778124242042495 2315 96-97 1.8545800841611282 2315 98-99 1.9854723881190068 2315 100-101 2.1540040772340436 2316 1 -0.8213547207837841 2316 2 -0.7819629364720164 2316 3 -0.7815744913793381 2316 4 -0.5172080316017258 2316 5 -0.7490619806966237 2316 6 -0.5093328817696658 2316 7 -0.6204606596220756 2316 8 -0.5536907066534624 2316 9 -0.7048354190065567 2316 10-11 -0.6030817672864188 2316 12-13 -0.5829111156377209 2316 14-15 -1.0952241119099995 2316 16-17 -0.8628587192727011 2316 18-19 -0.7417803330338515 2316 20-21 -0.6066005112636006 2316 22-23 -0.8105369759231564 2316 24-25 -0.9577658437819778 2316 26-27 -0.7073314106627961 2316 28-29 -0.85203735018559 2316 30-31 -0.7638224736003494 2316 32-33 -0.8043218619633237 2316 34-35 -0.6694766344408833 2316 36-37 -0.6717091818139593 2316 38-39 -0.689725701308852 2316 40-41 -0.9049581362635806 2316 42-43 -0.7862009692994079 2316 44-45 -0.5810509058992537 2316 46-47 -0.5145256917220777 2316 48-49 -0.40482060591855173 2316 50-51 -0.11071762440521127 2316 52-53 0.028316626678353884 2316 54-55 -0.1354697064734225 2316 56-57 0.08584229377142805 2316 58-59 0.19264921087631492 2316 60-61 -0.0610301120791199 2316 62-63 0.1620922882684681 2316 64-65 0.18595481917909495 2316 66-67 0.34156765148772905 2316 68-69 0.27840428772436354 2316 70-71 0.010402065073563449 2316 72-73 0.3295742046541186 2316 74-75 0.44964917858492015 2316 76-77 0.5477434137659856 2316 78-79 0.6945510581423058 2316 80-81 0.9237785070674711 2316 82-83 1.1559366400577957 2316 84-85 0.9927848032764643 2316 86-87 1.4230266335662058 2316 88-89 1.4479058367146074 2316 90-91 1.8741453999968485 2316 92-93 1.9091050927678381 2316 94-95 2.056054772968345 2316 96-97 2.085816589802942 2316 98-99 2.1577072311515906 2316 100-101 2.273366590715364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6008.0 3 1627.0 4 1773.0 5 1959.0 6 2428.0 7 2965.0 8 3955.0 9 5151.0 10 6653.0 11 9162.0 12 12620.0 13 16248.0 14 19534.0 15 24585.0 16 30065.0 17 36108.0 18 31511.0 19 24214.0 20 24941.0 21 26800.0 22 29636.0 23 32678.0 24 37002.0 25 41619.0 26 47262.0 27 53371.0 28 61035.0 29 68990.0 30 78897.0 31 91104.0 32 105426.0 33 123912.0 34 148395.0 35 180687.0 36 204010.0 37 205193.0 38 112622.0 39 7961.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69108895255595 18.95298996694693 14.851083388805254 21.504837691691865 2 15.878031633713144 21.27092885960923 39.76890116446443 23.08213834221319 3 18.70512302296168 26.755870809591485 30.07017589610525 24.46883027134158 4 12.697284283392548 17.312562979715054 36.64449293896992 33.34565979792248 5 13.32936185218474 38.22924453351877 32.55611238596119 15.885281228335302 6 30.12991917739763 36.41816176831132 17.72640917916077 15.725509875130278 7 27.773171166717255 34.72532951175833 20.175444085997952 17.326055235526464 8 27.149124225163778 33.151829828599915 19.905846252050846 19.793199694185457 9 28.443924517123005 16.242211955801775 19.977083096462746 35.33678043061247 10-11 25.270772713630592 27.147283615855155 26.125391553627953 21.456552116886304 12-13 26.755891389769022 25.17611000476928 27.024916289891866 21.043082315569833 14-15 23.58019746836235 25.78472892557415 26.886616572093995 23.7484570339695 16-17 21.382606684634403 29.224149911104284 27.09766639298763 22.295577011273682 18-19 21.37928445082268 26.36071467309682 31.53600377966158 20.723997096418916 20-21 22.852945954692302 25.42379643689464 31.329057231392188 20.39420037702087 22-23 22.171798216130547 25.598312818087145 31.314272572290104 20.915616393492208 24-25 21.629583225642566 25.943664535802146 31.50598508560014 20.920767152955143 26-27 21.079688768069833 26.361509301699698 31.559304724808634 20.999497205421836 28-29 21.03145608586471 26.333148190160294 31.756017805036418 20.879377918938584 30-31 21.214959607391144 26.111279160125793 31.451343725716193 21.22241750676687 32-33 21.037446017146223 26.311768353388022 31.116053292113143 21.534732337352615 34-35 21.649526471692603 26.481210104718595 30.93511410571989 20.934149317868915 36-37 21.83319678692659 25.96794540507869 30.82614661172825 21.37271119626647 38-39 21.285974561776754 26.286355217380418 30.86311311624492 21.564557104597903 40-41 21.308745523731556 26.168288139371477 31.01177028839749 21.51119604849948 42-43 21.603958625181303 26.242917105660762 30.38918537557167 21.763938893586268 44-45 21.1494930087477 26.67059450736666 30.299085082504206 21.880827401381435 46-47 21.54976179631623 26.961846478097335 29.547502948052482 21.94088877753395 48-49 21.693621596480607 26.81844750658426 29.340176480617945 22.147754416317184 50-51 21.83421811929021 27.270802897634905 28.75773340465845 22.137245578416437 52-53 21.98591303663241 27.338792277117825 28.524575804823165 22.150718881426602 54-55 21.510665615203635 27.438961919224177 28.587993769429797 22.46237869614239 56-57 21.623097671729287 27.4836986118175 28.56836364119819 22.324840075255022 58-59 21.677600124298348 26.813196897129565 28.777934541071115 22.73126843750098 60-61 21.744727342687074 27.13181467316342 28.99056274959469 22.132895234554812 62-63 21.63371601964526 27.15143741983921 28.834735821315732 22.3801107391998 64-65 21.515803886032245 26.944215322016362 28.86760106906034 22.67237972289105 66-67 21.567932815466456 27.13934205933546 28.71145096830887 22.58127415688921 68-69 21.682342541079272 26.9390503545809 28.803705587448388 22.57490151689144 70-71 21.649780991564246 27.236040803014422 28.49346546403431 22.620712741387027 72-73 21.78662136591567 27.169370477029215 28.265207531979836 22.77880062507528 74-75 21.55264445618756 27.42161578615455 28.159398636587717 22.866341121070167 76-77 21.50404538232024 27.194875718711998 28.396824176096334 22.904254722871435 78-79 21.794032907435195 27.14003126195916 28.334383045548822 22.731552785056824 80-81 21.592168408824826 27.575300101591495 28.112350757348047 22.720180732235633 82-83 21.85096073003125 27.25457996689592 27.855943809096505 23.038515493976323 84-85 21.820757864881493 26.828187401591226 27.973457002533863 23.377597730993422 86-87 22.02762439868473 26.844298512007015 27.842245946091083 23.285831143217177 88-89 21.98242305131199 26.768006490977136 27.96936913730179 23.280201320409084 90-91 21.94808801231766 26.81022490675059 27.960194464616073 23.28149261631568 92-93 22.260198795629897 26.2790761942155 28.000822796789528 23.459902213365076 94-95 22.078936891212976 26.37828770089532 28.081633355434505 23.461142052457205 96-97 22.441332473995647 26.256781363218263 27.943388001393295 23.358498161392795 98-99 22.547310692054765 26.176784051340718 27.517292765768403 23.75861249083611 100-101 23.121737827965553 25.62164258206674 27.83700999899274 23.419609590974968 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2774.0 1 1885.0 2 1328.0 3 1693.0 4 1471.0 5 1424.5 6 2411.5 7 4740.5 8 7260.0 9 7858.0 10 6202.0 11 4605.5 12 4441.5 13 4648.0 14 4785.0 15 4903.0 16 5031.5 17 5222.0 18 5507.0 19 5796.5 20 6081.5 21 6549.0 22 7268.5 23 8286.5 24 9380.5 25 10800.5 26 12339.5 27 14283.0 28 16329.5 29 18381.0 30 21341.0 31 24762.5 32 29027.5 33 32489.5 34 34583.0 35 36422.5 36 39229.5 37 42750.5 38 45267.0 39 46823.0 40 48945.5 41 54464.0 42 59509.5 43 62826.5 44 65391.0 45 67543.0 46 68145.0 47 68361.0 48 70686.5 49 70098.5 50 67619.0 51 64829.0 52 63228.0 53 63224.0 54 61673.0 55 57737.5 56 51637.0 57 46244.5 58 42831.0 59 38981.5 60 33682.0 61 28673.0 62 24396.0 63 19206.5 64 14726.5 65 11899.5 66 9483.5 67 7669.5 68 6051.0 69 4568.0 70 3760.0 71 3202.0 72 2795.5 73 2434.5 74 1933.5 75 1562.0 76 1154.5 77 758.5 78 463.5 79 288.0 80 160.0 81 76.5 82 51.0 83 40.0 84 28.5 85 21.0 86 12.5 87 6.0 88 8.0 89 7.5 90 5.0 91 4.5 92 3.5 93 2.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2986797406611098 2 0.034408907110548524 3 0.040821476162968925 4 0.042958999180442395 5 0.05771312147178365 6 0.055784137285283204 7 0.02992532224462856 8 0.03159363289241273 9 0.03889249197646848 10-11 0.030577006091419254 12-13 0.03271452910889272 14-15 0.027892068642641602 16-17 0.02145343223634957 18-19 0.02481612088578954 20-21 0.03688530572835315 22-23 0.030811612276263903 24-25 0.05044032974159953 26-27 0.042958999180442395 28-29 0.035086658311210835 30-31 0.03534733184992712 32-33 0.031332959353696455 34-35 0.030655208153034137 36-37 0.029560379290425775 38-39 0.018977033618544942 40-41 0.018533888602727273 42-43 0.019368043926619356 44-45 0.00914964120894131 46-47 0.01650063500074031 48-49 0.014441314044881727 50-51 0.006777512006623194 52-53 0.004874595173994373 54-55 0.007585599976643651 56-57 0.00503099929722414 58-59 0.0074031284995422575 60-61 0.006438636406292033 62-63 0.004587854281406469 64-65 0.008445822654407364 66-67 0.002476398617804628 68-69 0.007272791730184119 70-71 0.013528956659374758 72-73 0.01350288930550313 74-75 0.01040087419477944 76-77 0.009410314747657588 78-79 0.00636043434467715 80-81 0.006907848775981331 82-83 0.011339298934158035 84-85 0.004587854281406469 86-87 0.001094828862608362 88-89 0.019237707157261218 90-91 0.02791813599651323 92-93 0.01350288930550313 94-95 0.02145343223634957 96-97 0.018351417125625876 98-99 0.013242215766786854 100-101 0.10505143610265949 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1918108.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.0075502281731 #Duplication Level Percentage of deduplicated Percentage of total 1 69.24851647324168 27.012149845564476 2 13.826930757680486 10.787093920633863 3 5.956942901261032 6.970972482818966 4 3.2010514324346326 4.994606981346376 5 1.9063738493396258 3.718148684089558 6 1.230184007613706 2.879187868013215 7 0.8577903556300732 2.3422210267737786 8 0.632010620456274 1.9722548817749566 9 0.4531157521172155 1.5907441913899847 >10 2.3378217342750456 16.633400678256784 >50 0.20856053118302473 5.605511133816594 >100 0.12964022413924686 9.445774746385945 >500 0.008228573150046924 2.1001333258993267 >1k 0.0024281035524728626 1.7233279854812802 >5k 0.0 0.0 >10k+ 4.0468392541214375E-4 2.2244722477549117 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18741 0.9770565578163483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13376 0.6973538507737833 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10158 0.5295843612559876 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4164 0.2170889230429152 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2833 0.14769762703664235 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2555 0.13320417828401737 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2521 0.1314315982207467 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2488 0.12971115286521925 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.6067353871627666E-5 0.0 14-15 0.0 0.0 0.0 1.0426941548651066E-4 0.0 16-17 0.0 0.0 0.0 1.56404123229766E-4 0.0 18-19 0.0 0.0 0.0 2.0853883097302132E-4 0.0 20-21 0.0 0.0 0.0 3.3887560033115966E-4 0.0 22-23 0.0 0.0 0.0 9.905594471218513E-4 0.0 24-25 0.0 0.0 0.0 0.0030759477568520644 0.0 26-27 0.0 0.0 0.0 0.005943356682731108 0.0 28-29 0.0 0.0 0.0 0.014050303736807313 0.0 30-31 0.0 0.0 0.0 0.04027406173166474 0.0 32-33 0.0 0.0 0.0 0.0838586774050262 0.0 34-35 0.0 0.0 0.0 0.14563830608078376 0.0 36-37 0.0 0.0 0.0 0.24067987829673826 0.0 38-39 0.0 0.0 0.0 0.3871784070552857 0.0 40-41 0.0 0.0 0.0 0.5475969027812824 0.0 42-43 0.0 0.0 0.0 0.7039749586571767 0.0 44-45 0.0 0.0 0.0 0.892780802749376 0.0 46-47 0.0 0.0 0.0 1.1076018660054596 0.0 48-49 0.0 0.0 0.0 1.343224677651102 0.0 50-51 0.0 0.0 0.0 1.5955827304823296 0.0 52-53 0.0 0.0 0.0 1.8446041620179885 0.0 54-55 0.0 0.0 0.0 2.111168922709253 0.0 56-57 0.0 0.0 0.0 2.4222567238132573 0.0 58-59 0.0 0.0 0.0 2.7532860506290575 0.0 60-61 0.0 0.0 0.0 3.1165346268301892 0.0 62-63 0.0 0.0 0.0 3.471962996869832 0.0 64-65 0.0 0.0 0.0 3.841363468584668 0.0 66-67 0.0 0.0 0.0 4.248457333997877 0.0 68-69 0.0 0.0 0.0 4.699578960100266 0.0 70-71 0.0 0.0 0.0 5.179739618415647 0.0 72-73 0.0 0.0 0.0 5.662741618303036 0.0 74-75 0.0 0.0 0.0 6.156926513001354 0.0 76-77 0.0 0.0 0.0 6.6795248234197455 0.0 78-79 0.0 0.0 0.0 7.240807086983632 0.0 80-81 0.0 2.6067353871627666E-5 0.0 7.842936894064359 0.0 82-83 0.0 5.213470774325533E-5 0.0 8.457396559526366 5.213470774325533E-5 84-85 0.0 5.213470774325533E-5 0.0 9.065313319166595 5.213470774325533E-5 86-87 0.0 5.213470774325533E-5 0.0 9.692598122733443 5.213470774325533E-5 88-89 0.0 5.213470774325533E-5 0.0 10.353249139255976 5.213470774325533E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6535 0.0 58.367035 1 GTATCAA 12545 0.0 45.53123 1 TATCAAC 17085 0.0 33.41011 2 ATCAACG 17195 0.0 33.196377 3 TCAACGC 17510 0.0 32.5449 4 CAACGCA 17855 0.0 31.889437 5 TGGTATC 2610 0.0 31.503258 2 GTGGTAT 2725 0.0 31.144167 1 AACGCAG 18340 0.0 31.069609 6 GTACATG 14665 0.0 28.02518 1 ACGCAGA 20590 0.0 27.45882 7 TACATGG 14840 0.0 27.447123 2 CGCAGAG 20770 0.0 27.175104 8 ACATGGG 15280 0.0 25.599197 3 GCAGAGT 21745 0.0 25.125711 9 CATGGGA 9580 0.0 24.309748 4 AGTACTT 13050 0.0 23.605713 12-13 GAGTACT 12870 0.0 23.271488 12-13 ATGGGAT 3585 0.0 23.068 5 AGGTAAG 600 0.0 22.17975 2 >>END_MODULE