FastQCFastQC Report
Thu 26 May 2016
SRR938653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938653_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1918108
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175370.9142863696934688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139720.7284261365887635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103790.5411061316672471No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45000.234606184844649No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29920.15598704556781995No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.1459250469733717No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG26970.14060730678355965No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG22350.11652107180617566No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC21960.11448781820418873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67300.059.433081
GTATCAA128350.045.4299131
TATCAAC175800.033.1950262
ATCAACG177950.032.779423
TCAACGC180200.032.370134
CAACGCA182800.031.8837155
AACGCAG188500.030.8905756
GTGGTAT27450.028.2418141
TGGTATC27650.027.0054762
ACGCAGA215050.026.966417
CGCAGAG216750.026.7549068
GTACATG143800.026.1285631
TACATGG146400.025.3071772
GCAGAGT228000.025.0389739
GGACCGA5500.024.1792166
ACATGGG149250.023.9847553
AGTACTT135600.023.87003512-13
GAGTACT134050.023.13626512-13
AGGACCG7100.022.0958255
CATGGGA95450.021.9642964
Produced by FastQC (version 0.11.5)