##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938653_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1918108 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7674833742417 31.0 31.0 34.0 30.0 34.0 2 31.981213258064717 31.0 31.0 34.0 30.0 34.0 3 32.021391913281214 33.0 31.0 34.0 30.0 34.0 4 35.730357206163575 37.0 35.0 37.0 33.0 37.0 5 35.60860806586491 37.0 35.0 37.0 33.0 37.0 6 35.66135587777122 37.0 35.0 37.0 33.0 37.0 7 35.588926170997674 37.0 35.0 37.0 33.0 37.0 8 35.58668542125886 37.0 35.0 37.0 33.0 37.0 9 37.119101739839465 39.0 37.0 39.0 33.0 39.0 10-11 37.0638105362159 39.0 37.0 39.0 33.0 39.0 12-13 36.94938971111116 39.0 37.0 39.0 33.0 39.0 14-15 37.907291977302634 40.0 37.0 41.0 32.5 41.0 16-17 37.86660370531795 40.0 37.0 41.0 32.5 41.0 18-19 37.94756395364598 40.0 37.0 41.0 33.0 41.0 20-21 37.9767862393567 40.0 37.0 41.0 33.0 41.0 22-23 37.96580901596782 40.0 37.0 41.0 33.0 41.0 24-25 37.87112560919406 40.0 37.0 41.0 32.5 41.0 26-27 37.671308654152945 40.0 37.0 41.0 32.0 41.0 28-29 37.51340174797248 39.5 36.5 41.0 32.0 41.0 30-31 37.1957191670125 39.0 36.0 41.0 31.5 41.0 32-33 36.88474163081537 39.0 35.0 40.5 31.0 41.0 34-35 36.63346589451689 39.0 35.0 40.0 30.0 41.0 36-37 36.558626521551446 39.0 35.0 40.5 30.0 41.0 38-39 36.359634076913295 39.0 35.0 40.0 30.0 41.0 40-41 36.118022030042106 38.5 35.0 40.0 30.0 41.0 42-43 35.788491315400385 38.0 35.0 40.0 29.5 41.0 44-45 35.34017766465705 38.0 35.0 40.0 27.5 41.0 46-47 34.781088186900845 38.0 34.0 40.0 25.0 41.0 48-49 34.225480264927725 38.0 33.5 40.0 23.0 41.0 50-51 33.79787634481478 38.0 33.0 40.0 21.5 41.0 52-53 33.28320250997337 37.0 33.0 40.0 19.5 41.0 54-55 32.95846271429971 37.0 32.0 40.0 18.0 41.0 56-57 32.598732188177095 36.5 32.0 39.5 14.5 41.0 58-59 32.15507156009985 36.0 31.0 39.0 11.0 40.5 60-61 31.764114168753792 35.5 31.0 39.0 8.0 40.0 62-63 31.301812515249402 35.0 30.0 39.0 7.0 40.0 64-65 30.819076923718583 35.0 29.5 38.0 3.5 40.0 66-67 30.338787232001536 34.5 29.0 38.0 2.0 40.0 68-69 29.805099087225535 34.0 28.0 37.0 2.0 39.5 70-71 29.284941463150147 34.0 28.0 36.5 2.0 39.0 72-73 28.740107960552795 34.0 26.5 36.0 2.0 39.0 74-75 28.159092449434546 33.0 26.0 35.5 2.0 38.0 76-77 26.90060517968748 31.5 25.0 34.5 2.0 36.0 78-79 27.537537250248683 33.0 26.0 35.0 2.0 37.0 80-81 27.277294344218365 33.0 26.0 35.0 2.0 36.5 82-83 26.823871231442652 33.0 25.0 35.0 2.0 36.0 84-85 26.285362450915173 32.0 24.0 35.0 2.0 35.5 86-87 25.682458964771534 32.0 21.5 34.5 2.0 35.0 88-89 24.981842784660717 31.0 19.0 34.0 2.0 35.0 90-91 24.480261799648403 31.0 17.0 34.0 2.0 35.0 92-93 23.758585282997622 31.0 7.0 34.0 2.0 35.0 94-95 23.03039401326724 30.5 2.0 34.0 2.0 35.0 96-97 22.102917823188267 30.0 2.0 34.0 2.0 35.0 98-99 20.991388649648506 29.0 2.0 34.0 2.0 35.0 100-101 17.969811136807728 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3170294746825455 1101 2 0.38896952029090315 1101 3 0.35642962605007256 1101 4 0.37571775830586773 1101 5 0.2968417712377658 1101 6 0.28578249346517026 1101 7 0.38140291151742645 1101 8 0.3207320986379969 1101 9 0.4193082007578752 1101 10-11 0.43078471459041623 1101 12-13 0.4211094742910788 1101 14-15 0.6079690617381885 1101 16-17 0.49693327346935945 1101 18-19 0.6562768784574615 1101 20-21 0.6470947480525524 1101 22-23 0.6845054015553771 1101 24-25 0.6250357422203976 1101 26-27 0.7295252920759694 1101 28-29 0.7105464180513223 1101 30-31 0.6382720935437547 1101 32-33 0.5497011472095892 1101 34-35 0.5595988658981241 1101 36-37 0.7374710728212577 1101 38-39 0.9397922547728044 1101 40-41 1.1269631754828353 1101 42-43 1.0716662967483543 1101 44-45 1.0954187469381367 1101 46-47 1.1458352135288195 1101 48-49 1.2361690857130512 1101 50-51 1.380371573473397 1101 52-53 1.3127505767600525 1101 54-55 1.4018653579029419 1101 56-57 1.490044540337614 1101 58-59 1.4781047343519091 1101 60-61 1.5286193705908993 1101 62-63 1.6335319376609156 1101 64-65 1.752916563581966 1101 66-67 1.6945141396319805 1101 68-69 1.9057484513779954 1101 70-71 1.8145300894597582 1101 72-73 1.8888206049668348 1101 74-75 1.8168728578149747 1101 76-77 1.581604590267883 1101 78-79 1.9057167525331948 1101 80-81 2.2548579305458674 1101 82-83 2.4043667058941693 1101 84-85 2.530731886830708 1101 86-87 2.674938654792644 1101 88-89 2.7062943772499466 1101 90-91 2.9830854835721183 1101 92-93 3.322431013509494 1101 94-95 3.493516898100001 1101 96-97 3.6232960658667395 1101 98-99 4.097539209610524 1101 100-101 4.1945901634463745 1102 1 0.28628767564234536 1102 2 0.26498292813102253 1102 3 0.25594479652238533 1102 4 0.14370055185951003 1102 5 0.11696733940837589 1102 6 0.099901585970251 1102 7 0.21389226668429018 1102 8 0.26710778304934024 1102 9 0.39884137797638175 1102 10-11 0.2868188062360204 1102 12-13 0.38377400776418824 1102 14-15 0.460562427703465 1102 16-17 0.4298127043547382 1102 18-19 0.4872949077473123 1102 20-21 0.486069631600472 1102 22-23 0.5076109594122613 1102 24-25 0.358752881317578 1102 26-27 0.4527363824281281 1102 28-29 0.3511011892754681 1102 30-31 0.26186276272404285 1102 32-33 0.06777587661525075 1102 34-35 0.1431608060052625 1102 36-37 0.40438325400462816 1102 38-39 0.3832760106588182 1102 40-41 0.43396763067308797 1102 42-43 0.43851887813800516 1102 44-45 0.4229798472940445 1102 46-47 0.5095299216870899 1102 48-49 0.48777532128863044 1102 50-51 0.44794407246597956 1102 52-53 0.5764349289257922 1102 54-55 0.5647679383063107 1102 56-57 0.6377664333285082 1102 58-59 0.7379827308970945 1102 60-61 0.7803355064953301 1102 62-63 0.8697235953805666 1102 64-65 0.7121326219925841 1102 66-67 0.6003883826582417 1102 68-69 0.7977537660451794 1102 70-71 0.7814264900830885 1102 72-73 0.9437594975660346 1102 74-75 1.0182747210482432 1102 76-77 0.9522670497245223 1102 78-79 1.2294348638255954 1102 80-81 1.2588640088794385 1102 82-83 1.30025763122676 1102 84-85 1.501626968650097 1102 86-87 1.5788727343210311 1102 88-89 1.7731599212424882 1102 90-91 1.7549091527214031 1102 92-93 1.9239535557577128 1102 94-95 2.2456053565929004 1102 96-97 2.5835780165693976 1102 98-99 2.9572880732693037 1102 100-101 3.449529690085935 1103 1 0.27558227228689347 1103 2 0.30750795909528605 1103 3 0.2597739174149538 1103 4 0.19311707464346028 1103 5 0.2293958967612042 1103 6 0.24032825471773833 1103 7 0.3126777003526442 1103 8 0.25137734046366944 1103 9 0.39883476521256256 1103 10-11 0.5077689347550915 1103 12-13 0.4867124224334134 1103 14-15 0.652739098269457 1103 16-17 0.5495819255100329 1103 18-19 0.5205607468045415 1103 20-21 0.38635196355195944 1103 22-23 0.5095061775243579 1103 24-25 0.5148572558592264 1103 26-27 0.5118612691018924 1103 28-29 0.5566177883045782 1103 30-31 0.5659888959037787 1103 32-33 0.6015596603990403 1103 34-35 0.588451923013487 1103 36-37 0.5706203665545289 1103 38-39 0.9454895539958912 1103 40-41 0.7885350997104723 1103 42-43 0.8909959202751949 1103 44-45 1.0536262383451245 1103 46-47 1.1437731603014 1103 48-49 1.061353395215363 1103 50-51 0.9676454797282474 1103 52-53 1.1315495049781958 1103 54-55 1.1388702390852288 1103 56-57 1.0922006882386555 1103 58-59 1.1828706346311222 1103 60-61 1.2866860742341721 1103 62-63 1.3742764832737144 1103 64-65 1.2945903151828269 1103 66-67 1.4553714639019972 1103 68-69 1.5754005743616553 1103 70-71 1.3908845939259642 1103 72-73 1.4504355786041323 1103 74-75 1.6786139095430919 1103 76-77 1.560543948629256 1103 78-79 1.7152856983894509 1103 80-81 2.0199726255090873 1103 82-83 2.061445601022296 1103 84-85 2.1404179702655917 1103 86-87 2.2811328778047795 1103 88-89 2.448180272023155 1103 90-91 2.6779393922290993 1103 92-93 2.8305371903575463 1103 94-95 2.8300713461566396 1103 96-97 3.3946073792246096 1103 98-99 3.7546594335214323 1103 100-101 4.017414009040696 1104 1 0.24023276227274337 1104 2 0.24816674400971905 1104 3 0.2954726647468675 1104 4 0.16539438704693055 1104 5 0.12416669543937076 1104 6 0.12284104046017319 1104 7 0.19522674232062087 1104 8 0.296992940185028 1104 9 0.19519718120347562 1104 10-11 0.29184916699325925 1104 12-13 0.27658280487771236 1104 14-15 0.49974572851049714 1104 16-17 0.5125557974765869 1104 18-19 0.4878413625530129 1104 20-21 0.4977428639210899 1104 22-23 0.5033703536465666 1104 24-25 0.4815305023594192 1104 26-27 0.46932156078786846 1104 28-29 0.43795612024550934 1104 30-31 0.5138874602812038 1104 32-33 0.5621874137030076 1104 34-35 0.5295865321527842 1104 36-37 0.6033772465606759 1104 38-39 0.5850498372530808 1104 40-41 0.7187809107219536 1104 42-43 0.7486502602383567 1104 44-45 0.615733772450767 1104 46-47 0.7642635985740895 1104 48-49 0.9614963129180722 1104 50-51 0.8267184607213096 1104 52-53 0.8929694413159837 1104 54-55 0.8504030035573464 1104 56-57 1.014233999343098 1104 58-59 1.1300422152042344 1104 60-61 1.1219286438771157 1104 62-63 1.2566165096508932 1104 64-65 1.3113625380799014 1104 66-67 1.2892779761398678 1104 68-69 1.37079073473193 1104 70-71 1.4338993517591696 1104 72-73 1.6296033091999256 1104 74-75 1.773132959865496 1104 76-77 1.7429059456593414 1104 78-79 1.9556697416837245 1104 80-81 2.1032499217986818 1104 82-83 2.248065535376835 1104 84-85 2.2697767742687773 1104 86-87 2.408538338921989 1104 88-89 2.5025525249168368 1104 90-91 2.6346677419944555 1104 92-93 2.999195108841157 1104 94-95 3.3829324581629656 1104 96-97 3.5699011166278964 1104 98-99 3.779067775304224 1104 100-101 3.9448371024590436 1105 1 0.27785195593805057 1105 2 0.2136819022840406 1105 3 0.16426437091240587 1105 4 0.15941611607534867 1105 5 0.005774624954788976 1105 6 0.1865922254264376 1105 7 0.19206450882185777 1105 8 0.19273449671953102 1105 9 0.21239777972761686 1105 10-11 0.21108326056376825 1105 12-13 0.3170319767954055 1105 14-15 0.3841847980099331 1105 16-17 0.35121567033667844 1105 18-19 0.3603680359112502 1105 20-21 0.37213876347312436 1105 22-23 0.3819031795355272 1105 24-25 0.358008224993668 1105 26-27 0.4972623682378199 1105 28-29 0.6377635051411374 1105 30-31 0.4399769322044733 1105 32-33 0.4739225295973526 1105 34-35 0.6078849205986714 1105 36-37 0.7471241098852701 1105 38-39 0.6517948046316491 1105 40-41 0.7537769143651403 1105 42-43 0.74837492984188 1105 44-45 0.7856962717537357 1105 46-47 0.8457904392216804 1105 48-49 0.8410851528593284 1105 50-51 0.9300103434700588 1105 52-53 0.9170614318734351 1105 54-55 1.0163528818078689 1105 56-57 0.9188477003412601 1105 58-59 1.0528474718075174 1105 60-61 0.9843459285850109 1105 62-63 1.1111409896598197 1105 64-65 1.2023386714652737 1105 66-67 1.123620854260281 1105 68-69 1.2072502878834719 1105 70-71 1.1988833663319305 1105 72-73 1.2530140603291287 1105 74-75 1.3530356996933754 1105 76-77 1.30845665292156 1105 78-79 1.6161559960750722 1105 80-81 1.9329818870768456 1105 82-83 1.9545784352448656 1105 84-85 1.8644788133401349 1105 86-87 1.9694758098786984 1105 88-89 2.1793425210134174 1105 90-91 2.4383908595245174 1105 92-93 2.546547290108464 1105 94-95 2.55482924063104 1105 96-97 2.9029742220759545 1105 98-99 3.3020363713747827 1105 100-101 3.4620388627148095 1106 1 0.08111804314219029 1106 2 0.17298901064398464 1106 3 0.11581065898483445 1106 4 0.12784032377786048 1106 5 0.06904457674911413 1106 6 0.10771305827952204 1106 7 0.12872449720548929 1106 8 0.07487607857991208 1106 9 0.11066470312962906 1106 10-11 0.21139429227374507 1106 12-13 0.048437874024422456 1106 14-15 -0.05224128096782721 1106 16-17 0.048626733912577436 1106 18-19 0.243025743960672 1106 20-21 0.36264368009063475 1106 22-23 0.15100097852711514 1106 24-25 0.1527938249882439 1106 26-27 0.07530886874970832 1106 28-29 0.21647161897253397 1106 30-31 0.15910470436993052 1106 32-33 0.21296833742099608 1106 34-35 0.1227114716892217 1106 36-37 0.23137166739648052 1106 38-39 0.25232669428065435 1106 40-41 -0.013356384616280081 1106 42-43 0.13579458704595027 1106 44-45 -0.2889382024247027 1106 46-47 -0.4935174714362276 1106 48-49 0.03252418160420234 1106 50-51 -0.05183060265393635 1106 52-53 0.22885320617233162 1106 54-55 0.2293611545168588 1106 56-57 0.24101510828604233 1106 58-59 0.6174389552451416 1106 60-61 0.34119619718532235 1106 62-63 0.5265522295152714 1106 64-65 0.2566747247017034 1106 66-67 0.4205655962706345 1106 68-69 0.19836031796399922 1106 70-71 0.3206956444287421 1106 72-73 0.7146215603460746 1106 74-75 1.0363411503475284 1106 76-77 1.1012749279131384 1106 78-79 1.2531548705979283 1106 80-81 1.084615495677479 1106 82-83 1.4960199499834168 1106 84-85 1.5551296690869627 1106 86-87 1.6896471343935353 1106 88-89 2.1034355288171795 1106 90-91 2.0211086269213894 1106 92-93 1.676978762562527 1106 94-95 2.217277204263212 1106 96-97 2.8015952583285184 1106 98-99 3.114729742133619 1106 100-101 3.713368445587669 1107 1 0.1938331266660569 1107 2 0.10677509601014279 1107 3 0.16114828868239783 1107 4 0.1483222521333829 1107 5 0.028626772806937595 1107 6 0.13843942885592497 1107 7 0.05713245775056919 1107 8 0.13333556232059607 1107 9 0.21050621138034842 1107 10-11 0.18767470709192224 1107 12-13 0.22227862887749694 1107 14-15 0.31795092639631406 1107 16-17 0.3378145145304643 1107 18-19 0.29496403063382104 1107 20-21 0.1967123965910602 1107 22-23 0.16443973580625482 1107 24-25 0.2736329483715565 1107 26-27 0.30975920684225855 1107 28-29 0.3290292541600479 1107 30-31 0.22801089191564472 1107 32-33 0.31934187818125537 1107 34-35 0.4134551415169838 1107 36-37 0.4114329023586265 1107 38-39 0.3154507689205275 1107 40-41 0.34294144182080544 1107 42-43 0.5335272774942297 1107 44-45 0.3830870139229532 1107 46-47 0.5306093123316984 1107 48-49 0.36864093041511126 1107 50-51 0.31684397397457076 1107 52-53 0.6307578093356341 1107 54-55 0.5578843988140747 1107 56-57 0.4957178238885973 1107 58-59 0.5473050184802517 1107 60-61 0.7404453144312306 1107 62-63 1.0652090734747368 1107 64-65 1.04210728892377 1107 66-67 1.0740246171640422 1107 68-69 1.1444138627052745 1107 70-71 1.1633087089218286 1107 72-73 1.4180120370359646 1107 74-75 1.314324147532453 1107 76-77 1.2969884270545968 1107 78-79 1.465163001727646 1107 80-81 1.7398630333567233 1107 82-83 1.8699499835278068 1107 84-85 1.9057360792809455 1107 86-87 2.0901939630335633 1107 88-89 2.2744309768613675 1107 90-91 2.3105529801043616 1107 92-93 2.544331415266008 1107 94-95 2.781012076782229 1107 96-97 3.115944005457127 1107 98-99 3.535567608070579 1107 100-101 3.8089311581830536 1108 1 0.23451864330839456 1108 2 0.25857471935049503 1108 3 0.18923404002624977 1108 4 0.15963919819026984 1108 5 0.14475892328648854 1108 6 0.18850369236474052 1108 7 0.20218808906724206 1108 8 0.21019442475355277 1108 9 0.2539676567063651 1108 10-11 0.26725802857907155 1108 12-13 0.3637487877667027 1108 14-15 0.45949804463626975 1108 16-17 0.4831581306787882 1108 18-19 0.4299779372375241 1108 20-21 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42-43 -0.3824323378674137 2316 44-45 -0.30682771716013235 2316 46-47 -0.4510146295038382 2316 48-49 -0.21142941322468545 2316 50-51 0.03145181323742463 2316 52-53 -0.11187834935388707 2316 54-55 0.06373365535329611 2316 56-57 0.13114886019359773 2316 58-59 0.14176414720800778 2316 60-61 0.41273048096662635 2316 62-63 0.4273666950619841 2316 64-65 0.5195622167006846 2316 66-67 0.7608295325798125 2316 68-69 0.6952213808541039 2316 70-71 0.7640779948337091 2316 72-73 0.853713639940878 2316 74-75 0.897514416325297 2316 76-77 0.6846534299449907 2316 78-79 0.7203025264163898 2316 80-81 0.9374582164624883 2316 82-83 1.1426915014395185 2316 84-85 1.1815449070676927 2316 86-87 1.4159808716171618 2316 88-89 1.6069303542089486 2316 90-91 1.724004983482768 2316 92-93 1.5112187138000408 2316 94-95 2.3857270490225027 2316 96-97 2.555074562112985 2316 98-99 2.731191441458897 2316 100-101 3.2311584249365417 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 45.0 5 110.0 6 254.0 7 611.0 8 1266.0 9 2244.0 10 3872.0 11 6000.0 12 8460.0 13 12141.0 14 15034.0 15 20013.0 16 25947.0 17 33661.0 18 29253.0 19 20289.0 20 18784.0 21 19370.0 22 20785.0 23 23357.0 24 26849.0 25 31173.0 26 36604.0 27 43463.0 28 51758.0 29 60891.0 30 72356.0 31 85329.0 32 102138.0 33 123700.0 34 155919.0 35 201035.0 36 244186.0 37 268287.0 38 145376.0 39 7541.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.377872037378516 19.85697356821074 15.391268578864475 21.373885815546263 2 16.12004429239868 21.894433668064668 38.90621976477878 23.07930227475787 3 18.667183844045994 26.880659234327233 30.51153639218728 23.94062052943949 4 13.014595632779802 17.986422036715346 36.30019790335059 32.69878442715426 5 13.263635054202041 38.48250984780008 32.72882696888158 15.525028129116297 6 30.195588569569598 36.75835771499832 17.411271940891755 15.634781774540329 7 27.645575744431493 34.85971592840445 19.96415217495574 17.53055615220832 8 27.14221514117036 33.621985831871825 19.66166660062937 19.574132426328443 9 27.996911539913288 16.28135641997218 19.913060161367348 35.808671878747184 10-11 25.17650205306479 27.43031153615959 26.051478853119846 21.341707557655774 12-13 26.57973377932838 25.184687202180484 27.094120873277213 21.141458145213928 14-15 23.372015548655238 25.909568178642704 26.85072999017782 23.867686282524236 16-17 21.279562986025812 29.35999954121457 27.153841180997105 22.206596291762505 18-19 21.321166482804934 26.477028405074165 31.508262308483154 20.69354280363775 20-21 22.665668460795743 25.55275823884787 31.323887914549132 20.457685385807263 22-23 22.05235575890409 25.68142669755822 31.36815028142315 20.89806726211454 24-25 21.6088942761996 26.102563099962854 31.47849094475617 20.810051679081376 26-27 21.03132652494591 26.491028242389575 31.553364014788208 20.924281217876306 28-29 20.89095087450759 26.38388974969084 31.834964454556257 20.89019492124531 30-31 21.088567484208397 26.185777860266473 31.53612830977192 21.189526345753208 32-33 20.928979497355186 26.38296141899198 31.22013311130737 21.467925972345466 34-35 21.567204259024617 26.546712135195875 31.051850808995678 20.83423279678383 36-37 21.763146081709326 26.041856204475085 30.907414484451078 21.287583229364515 38-39 21.163028000289906 26.27507139419555 31.036179142205977 21.52572146330857 40-41 21.213123694580613 26.22324900441405 31.135249529970814 21.428377771034523 42-43 21.610295748869067 26.26067162305647 30.50416437369118 21.624868254383287 44-45 21.1184988110339 26.68286538484599 30.401480042522287 21.79715576159783 46-47 21.502163330825653 26.985903289024023 29.534509805761616 21.97742357438871 48-49 21.63955314299299 26.802505385515307 29.364639530203725 22.193301941287977 50-51 21.74812367186832 27.202013650951873 28.8366452775339 22.213217399645902 52-53 22.003166662148324 27.34848611235655 28.42425973928475 22.22408748621037 54-55 21.438527298391776 27.42580903312249 28.530074565685187 22.605589102800547 56-57 21.574723633914253 27.508226856881883 28.454941014791657 22.462108494412202 58-59 21.55895288482192 26.979424516242045 28.608946941465234 22.8526756574708 60-61 21.710247806692845 27.321871344053623 28.728205085427934 22.239675763825602 62-63 21.482549470624175 27.424785257138808 28.534472511453995 22.558192760783022 64-65 21.332583983800703 27.151677590625763 28.547610457805295 22.968127967768236 66-67 21.39384965890814 27.3335662020588 28.451075410365963 22.821508728667094 68-69 21.421738040136532 27.133631509882424 28.47474822780633 22.96988222217472 70-71 21.339622169346043 27.387795681994966 28.23409839279123 23.038483755867762 72-73 21.419414339547096 27.269554164833266 27.978638324849282 23.332393170770363 74-75 21.110985408537996 27.553531917910774 27.723777806046375 23.611704867504855 76-77 20.83321168568193 27.320828649898754 28.39592452562629 23.45003513879302 78-79 21.019932141464402 27.17805775274385 28.37918928444071 23.42282082135104 80-81 20.69665560888939 27.64063735756139 28.214927530111723 23.447779503437506 82-83 20.837671413921445 27.304257201561345 27.95884032613677 23.89923105838044 84-85 20.657976441293275 26.86151895421156 28.028870442754155 24.451634161741005 86-87 20.705565491714754 26.92230792431071 27.995778066434717 24.37634851753982 88-89 20.52852108876095 26.890574926216317 27.90138923428151 24.679514750741223 90-91 20.363861682302435 26.851221644014377 28.18040745188642 24.604509221796768 92-93 20.39837891536853 26.37552242494324 28.154940948027434 25.071157711660792 94-95 20.043162324540642 26.388425469264504 28.32551660281903 25.242895603375825 96-97 20.205640141222496 26.349246236395444 28.221429658809623 25.223683963572434 98-99 20.130487960010594 26.276674723216836 27.97592732004663 25.61690999672594 100-101 19.977925923068142 25.963811860463053 28.258572212834977 25.79969000363383 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2837.0 1 1893.0 2 1227.5 3 1648.5 4 1529.0 5 1467.0 6 2437.5 7 4814.5 8 7055.0 9 7436.0 10 5977.0 11 4506.5 12 4292.5 13 4491.0 14 4646.5 15 4839.5 16 5028.5 17 5175.5 18 5423.5 19 5668.0 20 6025.5 21 6555.0 22 7181.0 23 8073.0 24 9114.5 25 10398.5 26 12167.5 27 14361.0 28 16211.5 29 18304.5 30 21460.5 31 24905.5 32 29199.0 33 32693.5 34 34715.0 35 36668.5 36 39798.0 37 43057.0 38 45583.5 39 47379.0 40 49185.5 41 55212.5 42 60951.0 43 64470.0 44 67296.5 45 69167.0 46 69240.0 47 69414.5 48 71893.5 49 71245.5 50 68614.5 51 65376.5 52 62926.5 53 62434.5 54 61623.5 55 58023.5 56 51213.0 57 45527.0 58 42170.5 59 38444.5 60 32906.5 61 27794.0 62 23843.5 63 18335.5 64 13604.0 65 10846.5 66 8403.0 67 6954.5 68 5529.5 69 3935.0 70 3202.5 71 2813.0 72 2458.0 73 2278.5 74 1936.0 75 1572.5 76 1050.5 77 568.5 78 350.0 79 213.5 80 103.5 81 53.0 82 32.5 83 20.5 84 16.0 85 8.5 86 6.5 87 7.5 88 5.0 89 2.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.213470774325533E-5 2 0.04426236687402378 3 2.0853883097302132E-4 4 0.0 5 0.10134987185288835 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010687615087367345 26-27 0.011912780719333844 28-29 0.0 30-31 0.0 32-33 7.298859084055746E-4 34-35 0.01553614290749009 36-37 0.003388756003311597 38-39 0.012251656319665002 40-41 0.0074031284995422575 42-43 0.005865154621116225 44-45 0.0028674089258790432 46-47 5.213470774325533E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.6067353871627666E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.5640412322976598E-4 68-69 2.6067353871627667E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.213470774325533E-5 82-83 8.862900316353407E-4 84-85 0.0047442584046362354 86-87 0.0015379738784260324 88-89 5.213470774325533E-5 90-91 8.60222677763713E-4 92-93 4.952797235609256E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.001094828862608362 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1918108.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.7775656649746 #Duplication Level Percentage of deduplicated Percentage of total 1 67.16740495997648 24.702536464614763 2 14.510488936480398 10.673209193845908 3 6.323902668437355 6.977332369420888 4 3.50766745939185 5.160138812747136 5 2.0848146540438104 3.8337203919198775 6 1.3555394286788485 2.9912064209819107 7 0.9136471151591444 2.3521201740666036 8 0.6556029675200763 1.9289184950497456 9 0.485241202102685 1.6061391156314437 >10 2.605790076852454 17.443262693737978 >50 0.23175858124627308 5.923996050634394 >100 0.14547396262369586 10.061107355222001 >500 0.009536574849753577 2.338701966939735 >1k 0.0027044018230644477 1.8148549219230383 >5k 0.0 0.0 >10k+ 4.2701081416807066E-4 2.1927555732646526 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17537 0.9142863696934688 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13972 0.7284261365887635 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10379 0.5411061316672471 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4500 0.234606184844649 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2992 0.15598704556781995 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2799 0.1459250469733717 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2697 0.14060730678355965 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2235 0.11652107180617566 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2196 0.11448781820418873 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.6067353871627666E-5 0.0 14-15 0.0 0.0 0.0 1.0426941548651066E-4 0.0 16-17 0.0 0.0 0.0 2.0853883097302132E-4 0.0 18-19 0.0 0.0 0.0 3.12808246459532E-4 0.0 20-21 0.0 0.0 0.0 5.213470774325533E-4 0.0 22-23 0.0 0.0 0.0 0.0012512329858381278 0.0 24-25 0.0 0.0 0.0 0.0032584192339534584 0.0 26-27 0.0 0.0 0.0 0.006073693452089246 0.0 28-29 0.0 0.0 0.0 0.014337044629395217 0.0 30-31 0.0 0.0 0.0 0.04087361087071218 0.0 32-33 0.0 0.0 0.0 0.0847971021444048 0.0 34-35 0.0 0.0 0.0 0.14751515555954098 0.0 36-37 0.0 0.0 0.0 0.2440164995923066 0.0 38-39 0.0 0.0 0.0 0.3916880592750773 0.0 40-41 0.0 0.0 0.0 0.5535663268178852 0.0 42-43 0.0 0.0 0.0 0.7108828074331581 0.0 44-45 0.0 0.0 0.0 0.9005749415569926 0.0 46-47 0.0 0.0 0.0 1.116751507214401 0.0 48-49 0.0 0.0 0.0 1.3526349923987597 0.0 50-51 0.0 0.0 0.0 1.605983604677109 0.0 52-53 0.0 0.0 0.0 1.855213575043741 0.0 54-55 0.0 0.0 0.0 2.1220650766275932 0.0 56-57 0.0 0.0 0.0 2.433622090101287 0.0 58-59 0.0 0.0 0.0 2.7671278155348915 0.0 60-61 0.0 0.0 0.0 3.1329049250615713 0.0 62-63 0.0 0.0 0.0 3.489402056609951 0.0 64-65 0.0 0.0 0.0 3.860601175741929 0.0 66-67 0.0 0.0 0.0 4.270875258327477 0.0 68-69 0.0 0.0 0.0 4.726167661049326 0.0 70-71 0.0 0.0 0.0 5.2108379715845 0.0 72-73 0.0 0.0 0.0 5.69819321956845 0.0 74-75 0.0 0.0 0.0 6.2010846104598905 0.0 76-77 0.0 0.0 0.0 6.733379976518528 0.0 78-79 0.0 0.0 0.0 7.306366481970775 0.0 80-81 0.0 0.0 0.0 7.924136701374479 0.0 82-83 0.0 0.0 0.0 8.557755871932134 0.0 84-85 0.0 0.0 0.0 9.186344043192562 0.0 86-87 0.0 0.0 0.0 9.833492170409592 0.0 88-89 0.0 0.0 0.0 10.517186727754641 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6730 0.0 59.43308 1 GTATCAA 12835 0.0 45.429913 1 TATCAAC 17580 0.0 33.195026 2 ATCAACG 17795 0.0 32.77942 3 TCAACGC 18020 0.0 32.37013 4 CAACGCA 18280 0.0 31.883715 5 AACGCAG 18850 0.0 30.890575 6 GTGGTAT 2745 0.0 28.241814 1 TGGTATC 2765 0.0 27.005476 2 ACGCAGA 21505 0.0 26.96641 7 CGCAGAG 21675 0.0 26.754906 8 GTACATG 14380 0.0 26.128563 1 TACATGG 14640 0.0 25.307177 2 GCAGAGT 22800 0.0 25.038973 9 GGACCGA 550 0.0 24.179216 6 ACATGGG 14925 0.0 23.984755 3 AGTACTT 13560 0.0 23.870035 12-13 GAGTACT 13405 0.0 23.136265 12-13 AGGACCG 710 0.0 22.095825 5 CATGGGA 9545 0.0 21.964296 4 >>END_MODULE