FastQCFastQC Report
Thu 26 May 2016
SRR938652_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938652_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911560
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178590.9342631149427693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131440.6876059344200549No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95700.5006382221850216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41380.21647240996882128No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27980.1463725962041474No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG25350.1326141999204838No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.12685973759651803No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG23960.12534265207474524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68100.058.2601051
GTATCAA130500.046.5536771
TATCAAC175500.034.3356482
ATCAACG180750.033.338353
TCAACGC182350.033.0197644
CAACGCA185550.032.3734665
AACGCAG189750.031.7988456
ACGCAGA215500.027.8412467
CGCAGAG217550.027.5133888
GTACATG148500.027.1668281
GTGGTAT27600.026.9532151
TGGTATC27500.026.7856452
TACATGG149750.026.6572652
GCAGAGT224200.026.2933589
ACATGGG151850.025.1619533
GAGTACT131550.024.26576412-13
AGTACTT132200.023.55357412-13
CATGGGA94850.022.9973854
GTACTTT138400.022.8597114-15
AGAGTAC215100.022.17191510-11
Produced by FastQC (version 0.11.5)