##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938652_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1911560 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.220639163824313 31.0 31.0 34.0 28.0 34.0 2 31.354083052585324 31.0 31.0 34.0 28.0 34.0 3 31.379760509740734 31.0 31.0 34.0 28.0 34.0 4 35.086876687103725 37.0 35.0 37.0 32.0 37.0 5 35.09849599280169 37.0 35.0 37.0 32.0 37.0 6 35.104556487894705 37.0 35.0 37.0 32.0 37.0 7 35.03896189499676 37.0 35.0 37.0 32.0 37.0 8 35.01514312917198 37.0 35.0 37.0 32.0 37.0 9 36.494216242231474 39.0 37.0 39.0 32.0 39.0 10-11 36.34078213605642 39.0 35.0 39.0 32.0 39.0 12-13 36.16976291615225 38.5 35.0 39.0 31.5 39.0 14-15 37.25018702002553 39.0 36.5 41.0 31.5 41.0 16-17 37.21628879030739 39.0 36.5 41.0 31.5 41.0 18-19 37.29909001025341 39.5 37.0 41.0 32.0 41.0 20-21 37.27359434179414 40.0 37.0 41.0 31.0 41.0 22-23 37.13114602732847 39.0 36.0 41.0 31.0 41.0 24-25 36.98734410638431 39.0 36.0 41.0 31.0 41.0 26-27 36.772246489777984 39.0 36.0 41.0 30.0 41.0 28-29 36.53356028583984 39.0 35.5 40.5 30.0 41.0 30-31 36.20217178639436 38.5 35.0 40.0 30.0 41.0 32-33 35.83830536315888 38.0 35.0 40.0 29.0 41.0 34-35 35.50343776810563 38.0 35.0 40.0 27.5 41.0 36-37 35.23132049216346 38.0 34.0 40.0 26.5 41.0 38-39 34.97024367532278 38.0 34.0 40.0 25.5 41.0 40-41 34.65482747075687 38.0 33.5 40.0 24.5 41.0 42-43 34.174187574546444 38.0 33.0 40.0 23.5 41.0 44-45 33.58013847328883 37.5 33.0 40.0 21.0 41.0 46-47 32.9310495093013 37.0 32.0 40.0 16.5 41.0 48-49 32.34068300236456 37.0 31.0 40.0 10.5 41.0 50-51 31.559517095984432 36.0 30.5 38.5 8.0 40.5 52-53 31.573247243089412 36.0 30.5 39.0 6.5 40.0 54-55 31.624568153759235 36.0 31.0 39.0 2.0 40.0 56-57 31.26610674004478 36.0 30.0 39.0 2.0 40.5 58-59 30.88466749670426 35.0 29.5 39.0 2.0 40.0 60-61 30.396661104019756 35.0 28.5 39.0 2.0 40.0 62-63 29.743406170876142 35.0 28.0 38.0 2.0 40.0 64-65 29.15048389796815 34.0 26.5 38.0 2.0 40.0 66-67 28.537161009855822 34.0 26.0 37.5 2.0 39.5 68-69 27.781384052815504 33.5 24.5 36.5 2.0 39.0 70-71 27.046392475255814 33.0 23.0 36.0 2.0 39.0 72-73 26.234101728431227 32.0 21.0 35.5 2.0 38.5 74-75 25.382493094645213 31.5 18.0 35.0 2.0 37.5 76-77 24.512792954445583 31.0 10.5 35.0 2.0 37.0 78-79 23.587416298729835 30.0 4.5 34.0 2.0 36.0 80-81 22.688848636715562 29.5 2.0 34.0 2.0 35.5 82-83 21.736083879135364 29.0 2.0 34.0 2.0 35.0 84-85 20.798459635062464 27.5 2.0 33.5 2.0 35.0 86-87 19.843204503128334 26.0 2.0 33.0 2.0 35.0 88-89 18.789804400594278 25.0 2.0 32.5 2.0 34.5 90-91 17.66035724748373 22.0 2.0 32.0 2.0 34.0 92-93 16.4952562305133 17.0 2.0 31.0 2.0 34.0 94-95 15.208360710623783 4.5 2.0 31.0 2.0 34.0 96-97 13.742482841239616 2.0 2.0 30.5 2.0 34.0 98-99 12.011083094435957 2.0 2.0 29.0 2.0 34.0 100-101 8.526928529577937 2.0 2.0 12.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7198591423198479 1101 2 0.542340239724382 1101 3 0.5271174863276755 1101 4 0.4653736761623293 1101 5 0.48502844221693664 1101 6 0.5891263797972144 1101 7 0.48353449897254563 1101 8 0.5547322519352704 1101 9 0.5209030033507887 1101 10-11 0.6233749735854843 1101 12-13 0.4922570010197589 1101 14-15 0.7072769467390074 1101 16-17 0.7956470790014691 1101 18-19 0.7874944305488896 1101 20-21 0.8438216926433668 1101 22-23 0.6948727461097803 1101 24-25 0.7791796789365435 1101 26-27 0.6358444590600598 1101 28-29 0.7687539724923411 1101 30-31 0.8340475670271417 1101 32-33 0.6825376895714683 1101 34-35 0.7842153971129733 1101 36-37 0.8978129395259415 1101 38-39 0.9739177171253459 1101 40-41 0.9353477577449212 1101 42-43 0.9367549533426924 1101 44-45 1.1110680779061468 1101 46-47 1.4501598095328703 1101 48-49 1.2076373835983532 1101 50-51 1.1127890127705768 1101 52-53 1.229635156723262 1101 54-55 1.3791572267341188 1101 56-57 1.5673713109492162 1101 58-59 1.3373944777139286 1101 60-61 1.4633045281984778 1101 62-63 1.3631827063745057 1101 64-65 1.3944138333169853 1101 66-67 1.5081905967024483 1101 68-69 1.522644750039717 1101 70-71 1.6716765063093213 1101 72-73 1.834345082343404 1101 74-75 1.808372304428314 1101 76-77 2.3764168094894806 1101 78-79 2.213547522745767 1101 80-81 2.5722814517874006 1101 82-83 2.8992914844980113 1101 84-85 2.6443787739276807 1101 86-87 2.658434988115591 1101 88-89 2.9463048456497027 1101 90-91 2.8937577859102 1101 92-93 3.1195784522389722 1101 94-95 3.1294347618289695 1101 96-97 3.2606061932652946 1101 98-99 3.0115263731672304 1101 100-101 2.5309218311850383 1102 1 0.5301320571624792 1102 2 0.5130860830075186 1102 3 0.5093937701124887 1102 4 0.2952816407690122 1102 5 0.3606557828200465 1102 6 0.41379153219744325 1102 7 0.42897095597759716 1102 8 0.47613585759143007 1102 9 0.3916815925299346 1102 10-11 0.6043747224411078 1102 12-13 0.5620033313287252 1102 14-15 0.6402279902369443 1102 16-17 0.7276816536725192 1102 18-19 0.7547082959382792 1102 20-21 0.6975581936333768 1102 22-23 0.5672019089664957 1102 24-25 0.6504015864959882 1102 26-27 0.7203365022235886 1102 28-29 0.5432055134226346 1102 30-31 0.38381230996492377 1102 32-33 0.5530037358236584 1102 34-35 0.542126444829016 1102 36-37 0.8233510614378119 1102 38-39 0.949616030295438 1102 40-41 0.9563398196402062 1102 42-43 1.0083172239653209 1102 44-45 0.923515958511274 1102 46-47 0.9213281581268049 1102 48-49 0.9093087424559059 1102 50-51 1.0342932149293063 1102 52-53 0.9839073861221408 1102 54-55 0.9994092452549701 1102 56-57 1.0761610170825513 1102 58-59 1.4250025842657408 1102 60-61 1.253823411896434 1102 62-63 1.276989888548524 1102 64-65 1.4884018394388043 1102 66-67 1.698663483516814 1102 68-69 1.7492820795286441 1102 70-71 1.6980768443135688 1102 72-73 1.7348482036100599 1102 74-75 1.6710511407279718 1102 76-77 1.7580494144116336 1102 78-79 2.021522674715861 1102 80-81 2.1824155434564823 1102 82-83 2.2216633835241026 1102 84-85 2.4249993086570605 1102 86-87 2.5845163311413373 1102 88-89 2.652093029653887 1102 90-91 2.617061555434592 1102 92-93 2.8657262999594515 1102 94-95 3.15759068224534 1102 96-97 2.798660115765747 1102 98-99 2.5525524019919423 1102 100-101 2.0330603740759177 1103 1 0.3353064240364958 1103 2 0.25284951737708283 1103 3 0.3456537963220683 1103 4 0.1149993194311989 1103 5 0.1566626762270218 1103 6 0.20136785159370874 1103 7 0.34743773474705364 1103 8 0.15435381520403268 1103 9 0.3490701201551758 1103 10-11 0.3270392149930714 1103 12-13 0.3620902828514545 1103 14-15 0.43752707081115005 1103 16-17 0.2668098389008122 1103 18-19 0.461035897395881 1103 20-21 0.39038185124166347 1103 22-23 0.37625316398205655 1103 24-25 0.38421560300305657 1103 26-27 0.4613826512053265 1103 28-29 0.3618629191968452 1103 30-31 0.34099995409474815 1103 32-33 0.3952749148235952 1103 34-35 0.36762564848111623 1103 36-37 0.516608496740254 1103 38-39 0.49038754050815925 1103 40-41 0.5122654581230819 1103 42-43 0.5866732195700521 1103 44-45 0.6679941121861717 1103 46-47 0.7868123232811328 1103 48-49 0.8215687111768801 1103 50-51 0.8027300734903449 1103 52-53 0.792700922311866 1103 54-55 0.839618347008507 1103 56-57 1.0084273487474071 1103 58-59 0.9571632152816321 1103 60-61 1.0796848591199293 1103 62-63 1.0685103838912973 1103 64-65 1.0504327029830556 1103 66-67 1.3398989529439795 1103 68-69 1.506451497155961 1103 70-71 1.722756769944212 1103 72-73 1.7900506615094507 1103 74-75 1.429648301051948 1103 76-77 1.7525540259127546 1103 78-79 1.9693070114214244 1103 80-81 1.848907131174304 1103 82-83 1.8058407541779964 1103 84-85 2.1298870817442896 1103 86-87 2.0972229471657684 1103 88-89 2.1841357436911686 1103 90-91 2.428131117959339 1103 92-93 2.7173860875913007 1103 94-95 2.7103364819837683 1103 96-97 2.434051345355657 1103 98-99 2.2411869037812746 1103 100-101 1.7843182640610102 1104 1 0.44280214628631853 1104 2 0.4442075420684404 1104 3 0.3324139295302899 1104 4 0.33737977241274564 1104 5 0.2791516828690277 1104 6 0.38527063167135367 1104 7 0.28538551859647754 1104 8 0.3665854208782662 1104 9 0.5752149932945798 1104 10-11 0.5426967005959114 1104 12-13 0.5112908495383621 1104 14-15 0.5202155218056035 1104 16-17 0.6529664873525434 1104 18-19 0.5946304954713568 1104 20-21 0.6285393828418151 1104 22-23 0.6003447170353553 1104 24-25 0.6966578763742959 1104 26-27 0.7832203358845362 1104 28-29 0.5691525734810341 1104 30-31 0.5975361723795984 1104 32-33 0.7084669444090679 1104 34-35 0.6804156615443517 1104 36-37 0.6310277346567617 1104 38-39 0.6884252951032934 1104 40-41 0.8090879636887607 1104 42-43 0.6931413618305484 1104 44-45 0.6647209433392263 1104 46-47 0.8132065018610959 1104 48-49 0.7159386399730394 1104 50-51 0.5607934025919263 1104 52-53 0.5997394312629467 1104 54-55 0.7134486353196685 1104 56-57 1.0659957467529644 1104 58-59 1.1503675721471893 1104 60-61 1.2347381240092616 1104 62-63 1.2700126561585954 1104 64-65 1.223480117014077 1104 66-67 1.47233441871278 1104 68-69 1.7171688712613111 1104 70-71 1.718480859657376 1104 72-73 1.8334951243528224 1104 74-75 1.7832425311489075 1104 76-77 1.8951328193112182 1104 78-79 1.903921829916026 1104 80-81 2.0288385031821754 1104 82-83 2.2378558044990555 1104 84-85 2.3247629809917747 1104 86-87 2.6664838623283202 1104 88-89 2.8852488786840844 1104 90-91 2.6463350148517613 1104 92-93 2.7285137577353673 1104 94-95 2.763321707646588 1104 96-97 2.877125989796424 1104 98-99 2.75269267184442 1104 100-101 2.056856947801881 1105 1 0.4252365076341249 1105 2 0.36126998709922375 1105 3 0.16713717303851539 1105 4 0.22898408055756647 1105 5 0.3589878926243557 1105 6 0.40308570441094815 1105 7 0.36694325609954603 1105 8 0.43000656077762045 1105 9 0.4592339099143885 1105 10-11 0.527828698808051 1105 12-13 0.516193022354372 1105 14-15 0.5999846871385941 1105 16-17 0.593385623128313 1105 18-19 0.5421267827963518 1105 20-21 0.5093307953885002 1105 22-23 0.5751965704892257 1105 24-25 0.5947964925197482 1105 26-27 0.3607539662318686 1105 28-29 0.45836058420814396 1105 30-31 0.3332506640675277 1105 32-33 0.42739870327983454 1105 34-35 0.4194434453296978 1105 36-37 0.6058428218145124 1105 38-39 0.6259716134273248 1105 40-41 0.6165082673351137 1105 42-43 0.6260536850279195 1105 44-45 0.6422480855166626 1105 46-47 0.7065633367876387 1105 48-49 0.3673444101188039 1105 50-51 0.3628984532717432 1105 52-53 0.4750007603285056 1105 54-55 0.9251489683790588 1105 56-57 1.1024419598244641 1105 58-59 1.0808684588637476 1105 60-61 1.2248306885665485 1105 62-63 1.1124214973714537 1105 64-65 1.2227224429508965 1105 66-67 1.4272596605773344 1105 68-69 1.614666600401847 1105 70-71 1.5827807861247827 1105 72-73 1.4917588900866328 1105 74-75 1.4969058960580028 1105 76-77 1.6391730748009294 1105 78-79 2.0912122259827477 1105 80-81 2.162468129607774 1105 82-83 2.294537898591937 1105 84-85 2.352901452955674 1105 86-87 2.3914452937627857 1105 88-89 2.380190989790389 1105 90-91 2.330004291204542 1105 92-93 2.7056126806991614 1105 94-95 2.6438766624456758 1105 96-97 2.481895515583295 1105 98-99 2.475903018312694 1105 100-101 2.142132406659675 1106 1 0.20568209399243997 1106 2 0.11297284462178325 1106 3 -0.0061780348731765855 1106 4 0.1294118368167858 1106 5 0.16937697085806747 1106 6 -0.16668895525288718 1106 7 0.321982058714255 1106 8 0.05685027331434611 1106 9 0.23894414311769197 1106 10-11 0.17245059826733922 1106 12-13 0.29721393395971774 1106 14-15 0.32205469519536223 1106 16-17 -0.0686804257604905 1106 18-19 0.38840654923313167 1106 20-21 0.36168636189560033 1106 22-23 0.2107544670912631 1106 24-25 0.3349129695801025 1106 26-27 0.23647236141221128 1106 28-29 0.4387928810516044 1106 30-31 0.28657968753737606 1106 32-33 0.3410769364388315 1106 34-35 0.3314712868632981 1106 36-37 0.33730644038328705 1106 38-39 0.2137253254933711 1106 40-41 0.36145839012469594 1106 42-43 -0.0027619977070614254 1106 44-45 0.08839080383155107 1106 46-47 0.3211268454410501 1106 48-49 0.1900806935398549 1106 50-51 -0.06191085603341051 1106 52-53 0.14303297079012367 1106 54-55 -0.017679123440419886 1106 56-57 0.544141550341287 1106 58-59 0.4094622343872274 1106 60-61 0.6837060079994188 1106 62-63 0.6562038040436171 1106 64-65 0.589081697298365 1106 66-67 0.7486548104581452 1106 68-69 0.9868203600886538 1106 70-71 0.8259146752182431 1106 72-73 0.8876855153527679 1106 74-75 1.3137599079687305 1106 76-77 1.4128152601509036 1106 78-79 1.6936511288225553 1106 80-81 1.4071445714509174 1106 82-83 1.653545974740858 1106 84-85 1.5645805831569284 1106 86-87 1.3657246836633092 1106 88-89 1.3821135396053066 1106 90-91 1.7122648653669579 1106 92-93 1.9391101487697178 1106 94-95 2.0562551533658873 1106 96-97 1.896674840420566 1106 98-99 1.720210260821423 1106 100-101 1.2571505274668997 1107 1 0.1929636278283624 1107 2 0.14853006908423438 1107 3 0.21233025892538038 1107 4 0.17070629217890598 1107 5 0.1370506512949632 1107 6 0.3118612589283458 1107 7 0.2692073846962586 1107 8 0.26873033182494055 1107 9 0.36760151333328395 1107 10-11 0.3587481021466772 1107 12-13 0.2445015386625471 1107 14-15 0.4636526939026808 1107 16-17 0.43130138780632876 1107 18-19 0.5020232158561768 1107 20-21 0.34119967200961554 1107 22-23 0.30922824591806375 1107 24-25 0.3818887881658526 1107 26-27 0.4523712291431963 1107 28-29 0.4376820694133343 1107 30-31 0.35716646822468334 1107 32-33 0.3095468948062958 1107 34-35 0.2430098591105505 1107 36-37 0.37676380431867784 1107 38-39 0.5250168762596843 1107 40-41 0.4019194096639893 1107 42-43 0.5765639528938706 1107 44-45 0.2901185149838952 1107 46-47 0.4315411297721923 1107 48-49 0.28747087495933954 1107 50-51 0.5125915029693999 1107 52-53 0.5716062123975405 1107 54-55 0.5519303000103939 1107 56-57 0.8921148751614574 1107 58-59 0.8068023049872224 1107 60-61 0.769550682776277 1107 62-63 0.7904717543148436 1107 64-65 0.7281126613363291 1107 66-67 0.8613202873744576 1107 68-69 0.9601781513398073 1107 70-71 1.2087817467838207 1107 72-73 1.1081486544619743 1107 74-75 1.1082462786108565 1107 76-77 1.1591830761826998 1107 78-79 1.6602822948738485 1107 80-81 1.8924097662812613 1107 82-83 2.0031134254739413 1107 84-85 2.1457215136219467 1107 86-87 1.879856850622616 1107 88-89 2.037120565587049 1107 90-91 1.8080025672821591 1107 92-93 2.0193093098387145 1107 94-95 2.0660382768792456 1107 96-97 1.8625408679017053 1107 98-99 1.821038201462997 1107 100-101 1.674673744607679 1108 1 0.337146126907129 1108 2 0.31632339872038173 1108 3 0.23005913261744126 1108 4 0.2585093667213201 1108 5 0.12006366368299126 1108 6 0.18937341863707502 1108 7 0.11676081334848476 1108 8 0.19603969709610425 1108 9 0.2057476795818758 1108 10-11 0.2788532149170351 1108 12-13 0.20954205470184917 1108 14-15 0.2606796405272931 1108 16-17 0.4994640689777299 1108 18-19 0.2672042740109859 1108 20-21 0.42512669784218104 1108 22-23 0.319719078238343 1108 24-25 0.40173203718616435 1108 26-27 0.35448070753362515 1108 28-29 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2316 66-67 -0.591847067835328 2316 68-69 -0.508084729988564 2316 70-71 -0.46944607627139945 2316 72-73 -0.38768927010644916 2316 74-75 -0.5313751420736388 2316 76-77 -0.42172409501379704 2316 78-79 -0.8062065218440502 2316 80-81 -0.6170044546643219 2316 82-83 -0.45573034517240174 2316 84-85 -0.19101075511341747 2316 86-87 -0.10442992445974397 2316 88-89 -0.3449889122004741 2316 90-91 -0.39306419126606684 2316 92-93 -0.4344088798671386 2316 94-95 -0.6021941288824539 2316 96-97 -0.5078164126315716 2316 98-99 -0.8209679323133692 2316 100-101 -0.11306336496676472 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6074.0 3 1767.0 4 1719.0 5 2042.0 6 2514.0 7 3399.0 8 4470.0 9 5808.0 10 7958.0 11 10790.0 12 14463.0 13 18897.0 14 21840.0 15 26767.0 16 33477.0 17 39984.0 18 35418.0 19 29224.0 20 30890.0 21 33422.0 22 37215.0 23 42033.0 24 47145.0 25 52604.0 26 59297.0 27 66192.0 28 74402.0 29 83171.0 30 94213.0 31 106292.0 32 121371.0 33 138845.0 34 158756.0 35 174381.0 36 171233.0 37 122646.0 38 30480.0 39 361.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79479960486576 18.917025485738353 14.849674108781654 21.438500800614232 2 15.883880906606777 21.253894480381867 39.771841271602135 23.090383341409222 3 18.752652519546878 26.81432337006435 29.987822613098725 24.445201497290046 4 12.620312011811 17.274897279146895 36.72562921265401 33.379161496388086 5 13.398893122398803 38.27040036766803 32.42995384782082 15.900752662112344 6 30.187839821120882 36.349109477620225 17.773442815838674 15.68960788542022 7 27.83705851261467 34.71601940477374 20.0725324067048 17.374389675906787 8 27.176799920034373 33.12042310608895 19.88349510498367 19.819281868893004 9 28.48955138899646 16.239986268078095 19.897732767589314 35.37272957533612 10-11 25.256959782631167 27.169377453582914 26.10869959418658 21.464963169599343 12-13 26.765565141492726 25.141691570509316 27.022574890751887 21.07016839724607 14-15 23.61918551411112 25.75440360933227 26.86667406226747 23.759736814289138 16-17 21.408252681568175 29.2076466062161 27.09056883858057 22.293531873635146 18-19 21.349546929444273 26.312470860580273 31.57370919238479 20.76427301759066 20-21 22.849949658072603 25.39504283773572 31.312286360791486 20.442721143400185 22-23 22.181276802838386 25.54987192200717 31.35566434408107 20.91318693107337 24-25 21.667089703969836 25.891322152937146 31.523823978103238 20.917764164989784 26-27 21.1088067771491 26.30643176066465 31.5180816079611 21.06667985422515 28-29 21.021424252422243 26.29824826261383 31.84917125351618 20.831156231447757 30-31 21.20581380775417 26.038347226630336 31.51904553455157 21.236793431063926 32-33 21.039128216533012 26.25117410813569 31.196661477479363 21.513036197851935 34-35 21.661454301032364 26.4334310744555 30.96736500068943 20.937749623822704 36-37 21.767477201261563 25.96960377527328 30.87687294507842 21.386046078386737 38-39 21.2195864653983 26.24397066048442 30.961963167072938 21.574479707044343 40-41 21.358580733632234 26.119962298415416 31.071447537324225 21.450009430628118 42-43 21.63175902544099 26.161359343129604 30.448350616895105 21.758531014534306 44-45 21.16676293380379 26.63860793534197 30.31219327929817 21.882435851556075 46-47 21.55049678706517 26.92536708977329 29.513295705639603 22.01084041752194 48-49 21.719167108792174 26.77956825528379 29.328984283353794 22.17228035257024 50-51 21.895939906270314 27.200077432282722 28.725781157010953 22.178201504436007 52-53 22.01479916594795 27.291975505802974 28.470985666725003 22.22223966152408 54-55 21.5057540960364 27.33068748329123 28.65153725945252 22.51202116121985 56-57 21.611046367906205 27.423128888679614 28.608544271926696 22.357280471487485 58-59 21.66753378967807 26.747122080195407 28.771507724195764 22.813836405930754 60-61 21.79945621949605 27.067074807225783 28.96497026053228 22.168498712745883 62-63 21.64051664196327 27.08846606809976 28.81965717299302 22.45136011694395 64-65 21.52166145156759 26.878704346520443 28.806704066103144 22.792930135808817 66-67 21.60814483117791 27.020245153726574 28.69124132975533 22.680368685340188 68-69 21.726606359887185 26.80963499418504 28.741127804089988 22.72263084183779 70-71 21.686941139631084 27.081090654244484 28.503150846944624 22.72881735917981 72-73 21.8232386728382 27.014262641672904 28.225208582174865 22.937290103314034 74-75 21.542449874891602 27.26138826505454 28.12277234456933 23.073389515484536 76-77 21.51543449657723 27.05594923265663 28.339493529831834 23.089122740934307 78-79 21.857466929791336 27.005689059468825 28.142114526197716 22.994729484542123 80-81 21.698965365606483 27.43896138413055 27.91660769879324 22.94546555146973 82-83 21.858991631372284 27.031478492443643 27.74473461849365 23.364795257690428 84-85 21.871165283084018 26.6332967546082 27.770969222861225 23.724568739446557 86-87 22.05919857830083 26.659998310241274 27.60009008558916 23.68071302586874 88-89 21.999125705589858 26.563036705453563 27.712830649800612 23.725006939155964 90-91 22.026034316488 26.598600905345787 27.631308366468822 23.74405641169739 92-93 22.33956143850264 25.992165274771267 27.73215441864115 23.93611886808494 94-95 22.121431802431125 26.079292203760886 27.732911462943015 24.06636453086497 96-97 22.421653711090432 25.97788155726436 27.522190779090007 24.0782739525552 98-99 22.548112741610094 25.840037167563768 27.05941473643677 24.55243535438937 100-101 23.580766813324953 24.890111041273833 27.214382987638803 24.314739157762414 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2374.0 1 1729.5 2 1306.0 3 1560.5 4 1374.0 5 1328.0 6 2089.0 7 3998.0 8 6211.0 9 6932.5 10 5719.0 11 4408.5 12 4234.5 13 4312.5 14 4485.5 15 4721.5 16 4921.5 17 5161.0 18 5434.5 19 5672.0 20 5949.0 21 6602.5 22 7308.0 23 8235.0 24 9446.0 25 10696.5 26 12191.5 27 14175.0 28 16257.5 29 18552.0 30 21460.5 31 24813.0 32 28780.0 33 31882.0 34 34376.5 35 36702.0 36 39625.5 37 42865.5 38 45010.5 39 46662.5 40 48796.0 41 53919.5 42 58867.5 43 62201.0 44 64866.0 45 67001.5 46 67823.5 47 67876.0 48 69963.5 49 70098.0 50 67624.0 51 64710.5 52 63143.0 53 62697.0 54 61427.5 55 57684.0 56 51746.5 57 46770.0 58 43124.5 59 39155.5 60 34229.0 61 29730.5 62 25221.0 63 19638.0 64 15130.0 65 12199.0 66 9629.5 67 7810.5 68 6213.5 69 4781.5 70 3851.0 71 3159.0 72 2801.0 73 2467.0 74 2055.5 75 1668.0 76 1171.0 77 796.0 78 488.0 79 257.0 80 138.0 81 84.0 82 61.0 83 36.0 84 18.5 85 13.0 86 12.5 87 8.0 88 6.0 89 6.0 90 3.0 91 1.0 92 0.5 93 1.5 94 1.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3869614346397707 2 0.02730753939191027 3 0.033532821360564145 4 0.04179832178953316 5 0.05968946828768126 6 0.0521040406788173 7 0.036096172759421626 8 0.038659524158279106 9 0.03578229299629622 10-11 0.03695934210801649 12-13 0.028406118562849192 14-15 0.0268890330410764 16-17 0.02152692042101739 18-19 0.02558120069472054 20-21 0.0328527485404591 22-23 0.03217267572035406 24-25 0.04054280273703153 26-27 0.03494528029462847 28-29 0.029897047437694866 30-31 0.03277427859967775 32-33 0.027987612212015317 34-35 0.029269287911444057 36-37 0.03133566301868631 38-39 0.01470003557303982 40-41 0.012267467408817928 42-43 0.013313733285902615 44-45 0.011090418297097658 46-47 0.019826738370754778 48-49 0.017681893322731172 50-51 0.011116574944024776 52-53 0.008814790014438469 54-55 0.00727154784573856 56-57 0.008500910251313063 58-59 0.003478834041306577 60-61 0.006643788319487747 62-63 0.01135198476636883 64-65 0.006434535144070812 66-67 0.003426520747452343 68-69 0.00580677561782 70-71 0.012999853522777207 72-73 0.010619598652409551 74-75 0.011378141413295947 76-77 0.004969762916152252 78-79 0.00899788654292829 80-81 0.010907321768607838 82-83 0.01333988993282973 84-85 0.008239343782041893 86-87 0.002327941576513424 88-89 0.015929397978614323 90-91 0.02443030822992739 92-93 0.010331875536211262 94-95 0.020323714662370002 96-97 0.018283496202054866 98-99 0.011299671472514596 100-101 0.1234593734959928 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1911560.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.574024541856275 #Duplication Level Percentage of deduplicated Percentage of total 1 69.77459741653747 27.612616305601957 2 13.690890307062414 10.836072580231004 3 5.7769374829911495 6.8584999718598345 4 3.167432242484875 5.0139216519505325 5 1.864472802705905 3.689234622595352 6 1.2362333885830203 2.9353638275547955 7 0.8423707430179067 2.333520032027264 8 0.5878174607807982 1.860984209525676 9 0.4287162869149792 1.5269425973880193 >10 2.2912747610156194 16.5661498213041 >50 0.20427231777468752 5.602888209606177 >100 0.12405857512500468 9.245488274623668 >500 0.008128037992392666 2.085142419107204 >1k 0.002398437440378163 1.6937201148553067 >5k 1.332465244654535E-4 0.5046357876452455 >10k+ 2.66493048930907E-4 1.6348195741238816 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17859 0.9342631149427693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13144 0.6876059344200549 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9570 0.5006382221850216 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4138 0.21647240996882128 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2798 0.1463725962041474 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2535 0.1326141999204838 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2425 0.12685973759651803 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2396 0.12534265207474524 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.231329385423424E-5 7 0.0 0.0 0.0 5.231329385423424E-5 5.231329385423424E-5 8 0.0 0.0 0.0 5.231329385423424E-5 5.231329385423424E-5 9 0.0 0.0 0.0 1.0462658770846848E-4 5.231329385423424E-5 10-11 0.0 0.0 0.0 1.0462658770846848E-4 5.231329385423424E-5 12-13 0.0 0.0 0.0 1.0462658770846848E-4 5.231329385423424E-5 14-15 0.0 0.0 0.0 1.0462658770846848E-4 5.231329385423424E-5 16-17 0.0 0.0 0.0 2.6156646927117115E-4 5.231329385423424E-5 18-19 0.0 0.0 0.0 3.138797631254054E-4 5.231329385423424E-5 20-21 0.0 0.0 0.0 4.708196446881081E-4 5.231329385423424E-5 22-23 0.0 0.0 0.0 0.0015432421686999102 5.231329385423424E-5 24-25 0.0 0.0 0.0 0.0036357739228692794 5.231329385423424E-5 26-27 0.0 0.0 0.0 0.006486848437925045 5.231329385423424E-5 28-29 0.0 0.0 0.0 0.01587708468476009 5.231329385423424E-5 30-31 0.0 0.0 0.0 0.04208604490573145 5.231329385423424E-5 32-33 0.0 0.0 0.0 0.08806943020360333 5.231329385423424E-5 34-35 0.0 0.0 0.0 0.1534087342275419 5.231329385423424E-5 36-37 0.0 0.0 0.0 0.2489066521584465 5.231329385423424E-5 38-39 0.0 0.0 0.0 0.39378831948774823 5.231329385423424E-5 40-41 0.0 0.0 0.0 0.5548871079118626 5.231329385423424E-5 42-43 0.0 0.0 0.0 0.7107545669505535 5.231329385423424E-5 44-45 0.0 0.0 0.0 0.898480821946473 5.231329385423424E-5 46-47 0.0 0.0 0.0 1.122695599405721 5.231329385423424E-5 48-49 0.0 0.0 0.0 1.3533972253028939 5.231329385423424E-5 50-51 0.0 0.0 0.0 1.6096015819540062 5.231329385423424E-5 52-53 0.0 0.0 0.0 1.858612860700161 5.231329385423424E-5 54-55 0.0 0.0 0.0 2.1207547762037287 5.231329385423424E-5 56-57 0.0 0.0 0.0 2.4249827366130283 5.231329385423424E-5 58-59 0.0 0.0 0.0 2.7567536462365814 5.231329385423424E-5 60-61 0.0 0.0 0.0 3.1136872502040216 5.231329385423424E-5 62-63 0.0 0.0 0.0 3.466305007428488 5.231329385423424E-5 64-65 0.0 0.0 0.0 3.8339628366360463 5.231329385423424E-5 66-67 0.0 0.0 0.0 4.246191592207412 5.231329385423424E-5 68-69 0.0 0.0 0.0 4.685440164054489 5.231329385423424E-5 70-71 0.0 0.0 0.0 5.1641329594676595 5.231329385423424E-5 72-73 0.0 0.0 0.0 5.647664734562348 5.231329385423424E-5 74-75 0.0 0.0 0.0 6.129103977902865 5.231329385423424E-5 76-77 0.0 0.0 0.0 6.639917135742534 5.231329385423424E-5 78-79 0.0 0.0 0.0 7.194411893950491 5.231329385423424E-5 80-81 0.0 5.231329385423424E-5 0.0 7.783616522630731 5.231329385423424E-5 82-83 0.0 5.231329385423424E-5 0.0 8.382263700851661 5.231329385423424E-5 84-85 0.0 5.231329385423424E-5 0.0 8.97002971395091 5.231329385423424E-5 86-87 0.0 5.231329385423424E-5 0.0 9.576236163133775 5.231329385423424E-5 88-89 0.0 5.231329385423424E-5 0.0 10.194108476846136 5.231329385423424E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6810 0.0 58.260105 1 GTATCAA 13050 0.0 46.553677 1 TATCAAC 17550 0.0 34.335648 2 ATCAACG 18075 0.0 33.33835 3 TCAACGC 18235 0.0 33.019764 4 CAACGCA 18555 0.0 32.373466 5 AACGCAG 18975 0.0 31.798845 6 ACGCAGA 21550 0.0 27.841246 7 CGCAGAG 21755 0.0 27.513388 8 GTACATG 14850 0.0 27.166828 1 GTGGTAT 2760 0.0 26.953215 1 TGGTATC 2750 0.0 26.785645 2 TACATGG 14975 0.0 26.657265 2 GCAGAGT 22420 0.0 26.293358 9 ACATGGG 15185 0.0 25.161953 3 GAGTACT 13155 0.0 24.265764 12-13 AGTACTT 13220 0.0 23.553574 12-13 CATGGGA 9485 0.0 22.997385 4 GTACTTT 13840 0.0 22.85971 14-15 AGAGTAC 21510 0.0 22.171915 10-11 >>END_MODULE