FastQCFastQC Report
Thu 26 May 2016
SRR938652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1911560
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172420.9019858126347067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136670.7149657871058194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101770.5323923915545419No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45410.2375546673920777No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29850.15615518215488922No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG26560.13894410847684613No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.13826403565674109No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG21860.11435686036535606No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC21770.11388604072066794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68400.060.0813871
GTATCAA128300.044.9173471
TATCAAC172850.033.114492
ATCAACG174400.032.8184623
TCAACGC176800.032.3729674
CAACGCA180200.031.7885135
GTACGAC600.003952310431.6655063
AACGCAG186200.030.764186
ACGCAGA212300.026.803077
CGCAGAG213200.026.6676438
GTGGTAT27500.025.2231981
GTACATG141300.025.2173271
GCAGAGT222850.025.1505269
TACATGG143650.024.6679822
TGGTATC26750.024.1499122
AGGTAAG5000.023.7503742
GAGTACT131900.023.4244212-13
GGTAAGG5700.023.332483
ACATGGG144800.023.1914843
TCGTATC1850.023.10665794-95
Produced by FastQC (version 0.11.5)