##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938652_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1911560 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.771688568499027 31.0 31.0 34.0 30.0 34.0 2 32.00586432024106 31.0 31.0 34.0 30.0 34.0 3 32.03776235116868 33.0 31.0 34.0 30.0 34.0 4 35.72811264098433 37.0 35.0 37.0 33.0 37.0 5 35.63105264809894 37.0 35.0 37.0 33.0 37.0 6 35.67369373705246 37.0 35.0 37.0 33.0 37.0 7 35.59231674653163 37.0 35.0 37.0 33.0 37.0 8 35.57310311996485 37.0 35.0 37.0 33.0 37.0 9 37.08277375546674 39.0 37.0 39.0 33.0 39.0 10-11 37.01895702986043 39.0 37.0 39.0 33.0 39.0 12-13 36.894837462596 39.0 37.0 39.0 32.5 39.0 14-15 37.819675291385046 40.0 37.0 41.0 32.5 41.0 16-17 37.92858006026492 40.0 37.0 41.0 33.0 41.0 18-19 38.136249189143946 40.0 37.0 41.0 33.0 41.0 20-21 38.125229132227076 40.0 37.0 41.0 33.0 41.0 22-23 37.980279457615765 40.0 37.0 41.0 33.0 41.0 24-25 37.844827523070165 40.0 37.0 41.0 32.5 41.0 26-27 37.60446833999456 40.0 36.5 41.0 32.0 41.0 28-29 37.4295028667685 39.5 36.0 41.0 32.0 41.0 30-31 37.09593970369751 39.0 36.0 40.5 31.0 41.0 32-33 36.86542823662349 39.0 35.0 40.5 31.0 41.0 34-35 36.65427896587081 39.0 35.0 40.5 30.0 41.0 36-37 36.46796726234071 39.0 35.0 40.0 30.0 41.0 38-39 36.2051923559815 38.0 35.0 40.0 30.0 41.0 40-41 35.91627545041746 38.0 35.0 40.0 29.5 41.0 42-43 35.55189347967105 38.0 35.0 40.0 28.5 41.0 44-45 35.10065731653728 38.0 34.0 40.0 26.5 41.0 46-47 34.472012126221514 38.0 33.5 40.0 24.0 41.0 48-49 33.89987497122769 38.0 33.0 40.0 22.5 41.0 50-51 33.44441895624516 37.5 33.0 40.0 19.5 41.0 52-53 32.854764433237776 37.0 32.0 40.0 17.0 41.0 54-55 32.43385167088661 36.5 31.0 39.5 13.5 41.0 56-57 32.047726725815565 36.0 31.0 39.0 10.0 40.0 58-59 31.53884994454791 35.5 30.5 39.0 7.5 40.0 60-61 31.079638619766055 35.0 30.0 39.0 6.5 40.0 62-63 30.62406777710352 35.0 29.0 38.0 2.0 40.0 64-65 30.017153790621272 34.5 28.0 38.0 2.0 40.0 66-67 29.408515034840654 34.0 27.5 37.5 2.0 39.5 68-69 28.73252265165624 34.0 26.0 36.5 2.0 39.0 70-71 28.01388499445479 33.0 25.5 36.0 2.0 39.0 72-73 27.480751061959865 33.0 25.0 35.5 2.0 38.5 74-75 26.813690127435184 32.0 23.5 35.0 2.0 37.5 76-77 25.682330400301325 30.5 22.5 34.0 2.0 36.0 78-79 26.20558810604951 32.0 23.0 35.0 2.0 36.0 80-81 25.860615936721842 32.0 21.5 35.0 2.0 36.0 82-83 25.23533135240327 31.0 19.5 34.5 2.0 36.0 84-85 24.52394144049886 31.0 17.0 34.0 2.0 35.0 86-87 23.68110469982632 30.5 8.5 34.0 2.0 35.0 88-89 22.782842809014625 29.5 2.0 34.0 2.0 35.0 90-91 21.994797966059135 29.0 2.0 34.0 2.0 35.0 92-93 20.99393872020758 29.0 2.0 33.5 2.0 35.0 94-95 19.892844849233086 26.5 2.0 33.0 2.0 34.5 96-97 18.588096371549938 25.0 2.0 33.0 2.0 34.0 98-99 16.887641507459875 12.5 2.0 32.0 2.0 34.0 100-101 12.709138609303396 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3289360144704254 1101 2 0.36459239301951385 1101 3 0.2726120677073496 1101 4 0.18360300981434108 1101 5 0.18499308440492968 1101 6 0.2782242922462572 1101 7 0.280902294488655 1101 8 0.34457941449061025 1101 9 0.3829911352543718 1101 10-11 0.43514974835746756 1101 12-13 0.36844393356015814 1101 14-15 0.4517712300193111 1101 16-17 0.3729398163110176 1101 18-19 0.43661644778377706 1101 20-21 0.44870433130713394 1101 22-23 0.5075778868896137 1101 24-25 0.34324040105097 1101 26-27 0.4362214837373841 1101 28-29 0.4971676423442233 1101 30-31 0.5825698015114753 1101 32-33 0.3871969927327896 1101 34-35 0.24802648303154484 1101 36-37 0.3869434696048941 1101 38-39 0.47445327799677983 1101 40-41 0.35224594407927867 1101 42-43 0.45853681993070694 1101 44-45 0.537465413877797 1101 46-47 0.4961766472655782 1101 48-49 0.6717596433148074 1101 50-51 0.7313246102762676 1101 52-53 0.9561614483341145 1101 54-55 0.8631554696211552 1101 56-57 0.8979932255065322 1101 58-59 0.894123159610622 1101 60-61 0.9458584838166253 1101 62-63 0.9927550867765369 1101 64-65 1.0128182612128853 1101 66-67 1.222055911611541 1101 68-69 1.3703267235329477 1101 70-71 1.5944758264976286 1101 72-73 1.834657220683546 1101 74-75 1.718277782661655 1101 76-77 1.740100755068788 1101 78-79 1.6537372965121584 1101 80-81 1.7961246659077261 1101 82-83 1.7436396070959788 1101 84-85 2.0125835879273453 1101 86-87 1.8633300482400301 1101 88-89 2.0092845080215618 1101 90-91 2.289154278068935 1101 92-93 2.5597041267149763 1101 94-95 2.622630369253489 1101 96-97 2.728214107132196 1101 98-99 2.816535975988959 1101 100-101 2.356505500850556 1102 1 0.22185901582605183 1102 2 0.26756949448045475 1102 3 0.29635700492818984 1102 4 0.270236717829178 1102 5 0.11101927279651136 1102 6 0.2707135492335553 1102 7 0.2996285068846376 1102 8 0.35586217946526233 1102 9 0.3061576078969779 1102 10-11 0.3017449105675851 1102 12-13 0.355110387471008 1102 14-15 0.540750570966523 1102 16-17 0.44924434481867337 1102 18-19 0.41824371068476296 1102 20-21 0.3243528318642319 1102 22-23 0.4736230984267209 1102 24-25 0.3998613159657509 1102 26-27 0.38955684750127517 1102 28-29 0.39065016838483757 1102 30-31 0.3219898245168551 1102 32-33 0.1445828573285084 1102 34-35 0.3920636773742743 1102 36-37 0.30322532574218286 1102 38-39 0.37122669720946533 1102 40-41 0.4268677359180586 1102 42-43 0.4314281437945553 1102 44-45 0.37488273395921823 1102 46-47 0.4323426162319066 1102 48-49 0.2828976187026555 1102 50-51 0.5482504227838163 1102 52-53 0.7042258328708328 1102 54-55 0.5632686233423172 1102 56-57 0.6561294012576369 1102 58-59 0.6812727542414052 1102 60-61 0.6581538739992432 1102 62-63 0.7636576524729897 1102 64-65 0.7827942984322043 1102 66-67 0.8513133984195242 1102 68-69 1.0855036831875076 1102 70-71 1.359696771105721 1102 72-73 1.293141044072165 1102 74-75 1.3560887472641774 1102 76-77 1.3080384296384082 1102 78-79 1.3089524967130721 1102 80-81 1.370783574387108 1102 82-83 1.523560475805752 1102 84-85 1.5433272666512536 1102 86-87 1.677078745688764 1102 88-89 1.6832182891596474 1102 90-91 1.8565133493065211 1102 92-93 2.0424195662661937 1102 94-95 1.933247393511813 1102 96-97 1.8721697836193947 1102 98-99 1.8038899549622052 1102 100-101 1.6717298449266735 1103 1 0.2307926151748667 1103 2 0.25809301973428234 1103 3 0.16636284524835077 1103 4 0.12264647051056699 1103 5 0.06993908794449766 1103 6 0.1558062300189036 1103 7 0.15904272407922093 1103 8 0.26949403547197903 1103 9 0.18714377274034177 1103 10-11 0.2915511347548758 1103 12-13 0.3216098798727103 1103 14-15 0.43238454098621304 1103 16-17 0.22571033703941623 1103 18-19 0.29095576199595996 1103 20-21 0.32173840666540343 1103 22-23 0.2931170288053977 1103 24-25 0.3062113902436536 1103 26-27 0.48956514415295516 1103 28-29 0.43095425700091994 1103 30-31 0.39303072113012405 1103 32-33 0.5093020320011235 1103 34-35 0.6229901977846879 1103 36-37 0.5719310088832543 1103 38-39 0.6828188834621116 1103 40-41 0.5802597598960375 1103 42-43 0.5150525132163537 1103 44-45 0.7069803888159001 1103 46-47 0.6370667427643397 1103 48-49 0.7489937109143838 1103 50-51 0.8927411194281731 1103 52-53 0.9490649235578132 1103 54-55 0.8679059943359206 1103 56-57 0.9823606047686226 1103 58-59 1.0037975756020856 1103 60-61 0.9905167603978278 1103 62-63 1.122815457401071 1103 64-65 1.168165602992488 1103 66-67 1.0537435334262995 1103 68-69 1.02720239322149 1103 70-71 1.2174774625107858 1103 72-73 1.017965605819505 1103 74-75 1.1737626405104393 1103 76-77 1.1978807801908857 1103 78-79 1.35874024615158 1103 80-81 1.423277534816652 1103 82-83 1.4439485500549054 1103 84-85 1.3844836239116098 1103 86-87 1.4268837417102915 1103 88-89 1.4997600487553449 1103 90-91 1.6995243675212635 1103 92-93 1.9193524574598513 1103 94-95 1.6054385196222256 1103 96-97 1.4461272472741804 1103 98-99 1.5506621001627572 1103 100-101 1.5386290138242877 1104 1 0.1525199063010625 1104 2 0.18010917399087845 1104 3 0.11622761314590946 1104 4 0.12719836467994838 1104 5 0.10262661899268721 1104 6 0.1411570055336142 1104 7 0.08410022072141743 1104 8 0.11892826851276794 1104 9 0.15184359427076544 1104 10-11 0.25869065380695844 1104 12-13 0.23169709077959055 1104 14-15 0.3738437597678441 1104 16-17 0.35110729385714023 1104 18-19 0.34429700686725795 1104 20-21 0.2666189056442647 1104 22-23 0.22333174426601232 1104 24-25 0.17353765838958424 1104 26-27 0.3883278164128967 1104 28-29 0.31384049644453427 1104 30-31 0.3175040518876031 1104 32-33 0.6301029372282514 1104 34-35 0.5470605920736631 1104 36-37 0.5777929065484315 1104 38-39 0.42324868236185154 1104 40-41 0.5919513571067583 1104 42-43 0.5369104367136259 1104 44-45 0.7598671802272818 1104 46-47 0.8040268722927735 1104 48-49 0.9289471616328697 1104 50-51 0.8146680388960803 1104 52-53 0.9334092778475238 1104 54-55 1.2015263509070948 1104 56-57 1.2912261270221208 1104 58-59 1.3252856806967515 1104 60-61 1.3028799183894044 1104 62-63 1.2608754746960606 1104 64-65 1.297906546121407 1104 66-67 1.187599396243911 1104 68-69 1.4151142991661807 1104 70-71 1.5665897143552776 1104 72-73 1.728518309541606 1104 74-75 1.6641712806458564 1104 76-77 1.759458304063493 1104 78-79 1.6719377954147028 1104 80-81 1.870552653949698 1104 82-83 1.9730032132625723 1104 84-85 2.241767758518151 1104 86-87 1.9191644725156145 1104 88-89 1.9733879488859785 1104 90-91 2.23639305438995 1104 92-93 2.6083158619970277 1104 94-95 2.5108993207114416 1104 96-97 2.47723063083658 1104 98-99 2.4430615040375727 1104 100-101 2.1274949040562987 1105 1 0.19970187783962245 1105 2 0.20095494298318783 1105 3 0.16884234561703693 1105 4 0.1700880513734333 1105 5 0.11454610801325771 1105 6 6.246872384139124E-4 1105 7 0.16566805093322046 1105 8 0.1566807664581802 1105 9 0.24657952575569198 1105 10-11 0.19683676739319367 1105 12-13 0.2414805300863705 1105 14-15 0.4254787785835319 1105 16-17 0.21212564816889312 1105 18-19 0.2782791571909513 1105 20-21 0.305465741580079 1105 22-23 0.22814452451890332 1105 24-25 0.023162786350717113 1105 26-27 0.2138025341775105 1105 28-29 0.1916682571608277 1105 30-31 0.22433393207273156 1105 32-33 0.31101618318756863 1105 34-35 0.2651957478618385 1105 36-37 0.19926370257478965 1105 38-39 0.16771859888073237 1105 40-41 0.35936272149680093 1105 42-43 0.2478141666904108 1105 44-45 0.37848390240385044 1105 46-47 0.4434562017043717 1105 48-49 0.506448351130679 1105 50-51 0.44846635773917143 1105 52-53 0.3050848116797198 1105 54-55 0.41939404991368434 1105 56-57 0.4766306283702235 1105 58-59 0.35622199570460467 1105 60-61 0.5037212177535615 1105 62-63 0.634913423280917 1105 64-65 0.8830911706005544 1105 66-67 0.902952568958991 1105 68-69 0.9073948187965009 1105 70-71 0.9369027325077361 1105 72-73 1.0712933719001256 1105 74-75 0.8955040949255952 1105 76-77 0.9482943263828005 1105 78-79 1.0097090369620538 1105 80-81 1.106847476390115 1105 82-83 1.3245192822966985 1105 84-85 1.3040008424173948 1105 86-87 1.5825041498372947 1105 88-89 1.5927681123878585 1105 90-91 1.850992878488455 1105 92-93 1.8831633900042952 1105 94-95 1.7037371020456966 1105 96-97 1.8531948449750217 1105 98-99 1.8794579744731443 1105 100-101 2.017884482613617 1106 1 0.002460559630495851 1106 2 0.08683455659054573 1106 3 0.06732595659970997 1106 4 0.17319862844292544 1106 5 0.019937498068827608 1106 6 0.031241237667970267 1106 7 0.016357859813382447 1106 8 -0.03706402502102435 1106 9 -0.11858284166063271 1106 10-11 0.0031559317041995882 1106 12-13 0.07575238926181527 1106 14-15 0.16443602233815113 1106 16-17 -0.08295224776866661 1106 18-19 -0.07343962006294191 1106 20-21 -0.05448270617690554 1106 22-23 0.03315204140074712 1106 24-25 -0.023802635992829835 1106 26-27 -0.14888770661189454 1106 28-29 0.05528253452960996 1106 30-31 0.16042631635013294 1106 32-33 0.17315020240752688 1106 34-35 -0.045861959087751814 1106 36-37 0.0648719609008026 1106 38-39 0.11713081471352638 1106 40-41 0.3151217881298791 1106 42-43 0.30816199205511197 1106 44-45 0.45902514558258645 1106 46-47 0.21183712349834138 1106 48-49 0.46057377646437914 1106 50-51 0.7023517412108831 1106 52-53 0.48609077258086586 1106 54-55 0.2412132183776592 1106 56-57 0.5973239257480358 1106 58-59 0.8807659300100994 1106 60-61 0.7441396159794103 1106 62-63 0.5950585835441586 1106 64-65 0.8119092066871225 1106 66-67 0.7029934065306662 1106 68-69 0.7363770357644448 1106 70-71 0.4324625117910408 1106 72-73 0.606377414238306 1106 74-75 0.5053856798109884 1106 76-77 0.496205934497155 1106 78-79 1.0594090538508176 1106 80-81 0.9223339141633424 1106 82-83 1.0241239192299219 1106 84-85 1.1428881777866096 1106 86-87 1.232173434722963 1106 88-89 1.220047333400796 1106 90-91 1.1706707738722884 1106 92-93 1.2349083309905424 1106 94-95 0.9398120438348521 1106 96-97 1.2690327627295375 1106 98-99 1.7669885334356756 1106 100-101 1.8236439645440328 1107 1 0.07829563092393244 1107 2 0.09016463642768002 1107 3 0.07811796922293013 1107 4 0.02708934760514836 1107 5 0.10445129228214256 1107 6 0.05676007436426289 1107 7 -0.060692169886763736 1107 8 0.10192978112468154 1107 9 0.0804973696683362 1107 10-11 0.09955161615518904 1107 12-13 0.11141650808333026 1107 14-15 0.14244888629577446 1107 16-17 0.09707093666269628 1107 18-19 0.04822434698690614 1107 20-21 0.15079371452370083 1107 22-23 0.19569727851781238 1107 24-25 0.1515114397830999 1107 26-27 0.20039805066333827 1107 28-29 0.2131087193299379 1107 30-31 0.1454914604154638 1107 32-33 0.2607789793633515 1107 34-35 0.29100128678497583 1107 36-37 0.2852953253647783 1107 38-39 0.32746764315658794 1107 40-41 0.47470826265709576 1107 42-43 0.4112703020775328 1107 44-45 0.19175876274639592 1107 46-47 0.3371421714765077 1107 48-49 0.3272117460734094 1107 50-51 0.33253389338553774 1107 52-53 0.18437339760278348 1107 54-55 0.3081089381548239 1107 56-57 0.2658646672834877 1107 58-59 0.42246009438929377 1107 60-61 0.4223619510127641 1107 62-63 0.5213806325664088 1107 64-65 0.641331453797573 1107 66-67 0.7911213291166455 1107 68-69 0.7764227766064629 1107 70-71 0.5717503101335915 1107 72-73 0.8354838192645673 1107 74-75 0.814627789646579 1107 76-77 0.7775687656145323 1107 78-79 1.0382787735704042 1107 80-81 1.0415963561695598 1107 82-83 1.2895887984798584 1107 84-85 1.309333816051911 1107 86-87 1.7080385986756212 1107 88-89 1.7037117295981332 1107 90-91 1.6517712755038332 1107 92-93 1.7236913576840394 1107 94-95 1.4688684688792755 1107 96-97 1.623205175349618 1107 98-99 1.7100477270047456 1107 100-101 1.6963262410144502 1108 1 0.06457636992081817 1108 2 0.04315109517036575 1108 3 0.018549413929768832 1108 4 0.0502988741079875 1108 5 0.11186794946098644 1108 6 0.09257946060656508 1108 7 0.0994788311819974 1108 8 0.08016827913591129 1108 9 0.06315804738004971 1108 10-11 0.13312428732459125 1108 12-13 0.11936901268339284 1108 14-15 0.21691698703068596 1108 16-17 0.17051263923004 1108 18-19 0.0951939382272684 1108 20-21 0.05959314452014297 1108 22-23 0.10748555451412756 1108 24-25 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-0.6929787077699032 2316 54-55 -0.5944754581276115 2316 56-57 -0.5568500916468153 2316 58-59 -0.7704761696018956 2316 60-61 -0.5237312064265147 2316 62-63 -0.6606995068024482 2316 64-65 -0.5868545790682766 2316 66-67 -0.5195862839755669 2316 68-69 -0.3514058506398321 2316 70-71 -0.4800848761824277 2316 72-73 -0.5231262674657131 2316 74-75 -0.3958808225438766 2316 76-77 -0.33375108796628794 2316 78-79 -0.7716816195644078 2316 80-81 -1.3033000667039474 2316 82-83 -0.5156731265070356 2316 84-85 -0.609155073443457 2316 86-87 -0.7813868345493411 2316 88-89 -0.40283483149571353 2316 90-91 -0.46324214852842616 2316 92-93 -0.3463932347564267 2316 94-95 -0.0059078825358476195 2316 96-97 -0.07505009845856847 2316 98-99 -0.11060515515374547 2316 100-101 -0.50708270129007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 48.0 5 123.0 6 271.0 7 682.0 8 1458.0 9 2506.0 10 4302.0 11 6506.0 12 9202.0 13 12754.0 14 16210.0 15 21941.0 16 28223.0 17 36250.0 18 32839.0 19 24111.0 20 23217.0 21 24578.0 22 26651.0 23 29906.0 24 34217.0 25 38987.0 26 44520.0 27 52090.0 28 61359.0 29 71087.0 30 83368.0 31 97826.0 32 115556.0 33 138934.0 34 169305.0 35 207007.0 36 230085.0 37 201468.0 38 62914.0 39 1042.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4051642266349 19.870818461950627 15.438365891218137 21.285651420196334 2 16.139830255746475 21.91319625018181 38.90446836941088 23.042505124660842 3 18.71499860582957 26.838905568363785 30.55644925455041 23.889646571256236 4 13.000272029128043 18.01481512481952 36.25295570110276 32.731957144949675 5 13.249774921699956 38.544547716861175 32.63821063143814 15.567466730000726 6 30.1655192617548 36.80977840088723 17.397884450396532 15.626817886961433 7 27.659555546255415 34.83348678566197 19.970547615560065 17.536410052522548 8 27.067892192764027 33.64686434116638 19.729435644185898 19.555807821883697 9 28.030327205499617 16.266790684632532 19.92685520815296 35.77602690171489 10-11 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26.530461694934942 31.052818687431643 20.865122442952256 36-37 21.72441546490906 26.040620117648565 30.942216500252023 21.29274791719035 38-39 21.199952074955604 26.239903563928134 31.013468193152576 21.54667616796368 40-41 21.253163070147234 26.155885828373183 31.15605456032769 21.434896541151893 42-43 21.65605695344282 26.21119711884553 30.491259019302564 21.64148690840909 44-45 21.140055970388374 26.700815282285834 30.343703383621584 21.815425363704204 46-47 21.50736087472035 27.019378680077704 29.473892913090072 21.99936753211187 48-49 21.617343949444432 26.828061897089288 29.33677206051602 22.21782209295026 50-51 21.810746196823537 27.15839419113185 28.796218795120215 22.234640816924397 52-53 21.969961706668897 27.318682123501226 28.428508652618806 22.282847517211074 54-55 21.442120571679677 27.373951118458223 28.57362572977045 22.610302580091655 56-57 21.599269706417797 27.530629433551656 28.35275900311787 22.51734185691268 58-59 21.56003473602712 26.975716168992864 28.57325953671347 22.890989558266547 60-61 21.684200338990145 27.34766368829647 28.709509510556824 22.258626462156563 62-63 21.42137835066647 27.387787984682667 28.56308460105882 22.62774906359204 64-65 21.303626698515007 27.154111603640903 28.498772860588435 23.043488837255655 66-67 21.34917072619526 27.318561043591806 28.42527956341235 22.90698866680059 68-69 21.46438958525859 27.123804073206927 28.38794155813073 23.023864783403752 70-71 21.36967224928128 27.328001037896023 28.188764200120374 23.113562512702323 72-73 21.387753906306195 27.27897396702635 27.901607930500617 23.43166419616684 74-75 21.125415890686142 27.484567578313 27.730073866370926 23.659942664629934 76-77 20.884931678838225 27.21664504383854 28.413782460398835 23.484640816924397 78-79 21.00603695411078 27.163102387578732 28.29301722153634 23.537843436774153 80-81 20.694349844472416 27.664868133742214 28.103724815046156 23.537057206739213 82-83 20.871550874035787 27.255556398391867 27.902941876554703 23.96995085101764 84-85 20.657769211834708 26.822606242239182 27.995122016296044 24.524502529630062 86-87 20.734109052518907 26.861110028467568 27.895756859825678 24.509024059187848 88-89 20.534171043545584 26.92042101738894 27.76802716106217 24.777380778003305 90-91 20.391607462393374 26.8126891971176 28.090852805721482 24.70485053476754 92-93 20.462530407261124 26.33302817085611 28.04056917161466 25.163872250268106 94-95 20.070848042118566 26.35651270483313 28.146965090219695 25.425674162828603 96-97 20.176097101894317 26.30156851031017 28.08286325454773 25.439471133247782 98-99 20.126048881541777 26.279086191382955 27.73737157086359 25.85749335621168 100-101 20.063602552576864 25.83389417587795 28.017662028130452 26.08484124341473 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2735.0 1 1861.5 2 1263.5 3 1596.5 4 1459.0 5 1412.0 6 2264.5 7 4506.5 8 6699.5 9 7092.5 10 5784.5 11 4466.5 12 4241.0 13 4383.0 14 4574.0 15 4812.0 16 5053.0 17 5134.0 18 5333.0 19 5698.5 20 6080.5 21 6611.0 22 7313.5 23 8165.0 24 9089.5 25 10525.5 26 12192.5 27 14166.5 28 16121.5 29 18417.0 30 21473.0 31 24856.0 32 29206.0 33 32286.0 34 34059.0 35 36627.0 36 40053.5 37 43117.5 38 45435.5 39 47388.5 40 49626.5 41 54836.0 42 59999.0 43 63845.0 44 66831.0 45 68712.5 46 68948.5 47 69144.0 48 71057.0 49 70782.0 50 68476.0 51 65046.0 52 62807.5 53 62225.5 54 61367.0 55 57673.0 56 51145.0 57 45648.5 58 41852.5 59 38136.5 60 33064.0 61 28106.0 62 23919.5 63 18597.0 64 13995.0 65 11109.0 66 8650.0 67 7038.0 68 5614.5 69 4123.0 70 3322.5 71 2855.0 72 2476.5 73 2272.5 74 1977.0 75 1615.0 76 1079.0 77 605.5 78 343.5 79 202.5 80 115.5 81 53.5 82 30.5 83 17.5 84 10.5 85 6.5 86 2.5 87 4.0 88 4.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02359329552825964 2 0.011195044884806127 3 0.001517085521772793 4 0.0 5 1.569398815627027E-4 6 0.0 7 0.0 8 0.0 9 4.708196446881081E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014124589340643245 26-27 0.008631693485948649 28-29 2.615664692711712E-4 30-31 0.0 32-33 2.0925317541693693E-4 34-35 0.010488815417773965 36-37 0.0010201092301575676 38-39 0.012659817112724686 40-41 0.01279060034736027 42-43 0.0054405825608403605 44-45 7.062294670321622E-4 46-47 2.6156646927117116E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.6156646927117116E-5 66-67 4.1850635083387386E-4 68-69 0.0010201092301575676 70-71 2.6156646927117116E-5 72-73 1.8309652848981983E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0462658770846846E-4 82-83 0.001307832346355856 84-85 0.00598987214630982 86-87 0.004525099918391261 88-89 0.0 90-91 0.0014909288748456758 92-93 5.231329385423424E-4 94-95 2.0925317541693693E-4 96-97 5.231329385423423E-5 98-99 0.0 100-101 7.846994078135135E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1911560.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.049150342365586 #Duplication Level Percentage of deduplicated Percentage of total 1 67.31716212783074 24.940436602954005 2 14.587497282934786 10.80908759908601 3 6.263887030885041 6.962150770045616 4 3.372417347957742 4.997807893667529 5 2.07898971983233 3.8512401345150256 6 1.3455983428051415 2.9911965181815408 7 0.9473593903343107 2.456920233652338 8 0.6587231030703746 1.9524105023715113 9 0.4855667685987435 1.619085258996434 >10 2.556554808214337 17.23747858103225 >50 0.2302817616219456 5.9288579603472655 >100 0.14362757774130647 10.011505973435447 >500 0.00921560897992993 2.2925743712015114 >1k 0.0026937933941333647 1.7910917839209333 >5k 0.0 0.0 >10k+ 4.2533579907368915E-4 2.158155816592629 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17242 0.9019858126347067 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13667 0.7149657871058194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10177 0.5323923915545419 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4541 0.2375546673920777 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2985 0.15615518215488922 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2656 0.13894410847684613 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2643 0.13826403565674109 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 2186 0.11435686036535606 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2177 0.11388604072066794 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.8309652848981983E-4 0.0 18-19 0.0 0.0 0.0 2.615664692711712E-4 0.0 20-21 0.0 0.0 0.0 4.708196446881081E-4 0.0 22-23 0.0 0.0 0.0 0.0014647722279185586 0.0 24-25 0.0 0.0 0.0 0.0034265207474523424 0.0 26-27 0.0 0.0 0.0 0.0062775952625081085 0.0 28-29 0.0 0.0 0.0 0.015641674862416036 0.0 30-31 0.0 0.0 0.0 0.041929105024168745 0.0 32-33 0.0 0.0 0.0 0.08778170708740504 0.0 34-35 0.0 0.0 0.0 0.15333026428676055 0.0 36-37 0.0 0.0 0.0 0.2492205319215719 0.0 38-39 0.0 0.0 0.0 0.395069995187177 0.0 40-41 0.0 0.0 0.0 0.5575289292515013 0.0 42-43 0.0 0.0 0.0 0.7144688108142041 0.0 44-45 0.0 0.0 0.0 0.9035813680972609 0.0 46-47 0.0 0.0 0.0 1.1282408085542697 0.0 48-49 0.0 0.0 0.0 1.3590470610391512 0.0 50-51 0.0 0.0 0.0 1.6157222373349516 0.0 52-53 0.0 0.0 0.0 1.8644719496118354 0.0 54-55 0.0 0.0 0.0 2.1258291657075894 0.0 56-57 0.0 0.0 0.0 2.4291939567682936 0.0 58-59 0.0 0.0 0.0 2.759029274519241 0.0 60-61 0.0 0.0 0.0 3.1150735524911592 0.0 62-63 0.0 0.0 0.0 3.4682405993010943 0.0 64-65 0.0 0.0 0.0 3.835244512335475 0.0 66-67 0.0 0.0 0.0 4.248388750549289 0.0 68-69 0.0 0.0 0.0 4.689599070915901 0.0 70-71 0.0 0.0 0.0 5.173549352361421 0.0 72-73 0.0 0.0 0.0 5.663254096130909 0.0 74-75 0.0 0.0 0.0 6.1556268178869615 0.0 76-77 0.0 0.0 0.0 6.679936805541024 0.0 78-79 0.0 0.0 0.0 7.25203498713093 0.0 80-81 0.0 0.0 0.0 7.860987884241143 0.0 82-83 0.0 0.0 0.0 8.482077465525538 0.0 84-85 0.0 0.0 0.0 9.095476992613364 0.0 86-87 0.0 0.0 0.0 9.72755236560715 0.0 88-89 0.0 0.0 0.0 10.386464458348154 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6840 0.0 60.081387 1 GTATCAA 12830 0.0 44.917347 1 TATCAAC 17285 0.0 33.11449 2 ATCAACG 17440 0.0 32.818462 3 TCAACGC 17680 0.0 32.372967 4 CAACGCA 18020 0.0 31.788513 5 GTACGAC 60 0.0039523104 31.665506 3 AACGCAG 18620 0.0 30.76418 6 ACGCAGA 21230 0.0 26.80307 7 CGCAGAG 21320 0.0 26.667643 8 GTGGTAT 2750 0.0 25.223198 1 GTACATG 14130 0.0 25.217327 1 GCAGAGT 22285 0.0 25.150526 9 TACATGG 14365 0.0 24.667982 2 TGGTATC 2675 0.0 24.149912 2 AGGTAAG 500 0.0 23.750374 2 GAGTACT 13190 0.0 23.42442 12-13 GGTAAGG 570 0.0 23.33248 3 ACATGGG 14480 0.0 23.191484 3 TCGTATC 185 0.0 23.106657 94-95 >>END_MODULE