FastQCFastQC Report
Thu 26 May 2016
SRR938651_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938651_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541665
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105390.6836115498503241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78460.5089302799246269No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58320.3782923008565415No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.1228541868693912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17070.11072444402642598No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG15900.10313524663269906No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45450.055.140111
GTATCAA85950.044.705031
TATCAAC113600.033.6146162
ATCAACG114450.033.3234143
TCAACGC115800.032.811734
CAACGCA117300.032.311065
AACGCAG121500.031.367416
ACGCAGA137500.027.5845177
CGCAGAG138700.027.2773278
GTACATG90300.025.5414121
GCAGAGT144450.025.1385969
TACATGG92900.024.2635042
GAGTACT79550.024.13671512-13
AGTACTT81900.023.18301412-13
GTACTTT84700.022.68982314-15
ACATGGG95900.022.512733
AGAGTAC130900.021.82192210-11
TAAGGTG6350.021.717775
GTAAGGT5050.021.658453
CAGAGTA140400.021.20860510-11
Produced by FastQC (version 0.11.5)