##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938651_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1541665 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.244442858857145 31.0 31.0 34.0 28.0 34.0 2 31.242244586210365 31.0 31.0 34.0 28.0 34.0 3 31.2706346709564 31.0 31.0 34.0 28.0 34.0 4 34.95867390126908 37.0 35.0 37.0 32.0 37.0 5 34.94892470154022 37.0 35.0 37.0 32.0 37.0 6 34.99408950712379 37.0 35.0 37.0 32.0 37.0 7 34.90363081473602 37.0 35.0 37.0 32.0 37.0 8 34.88476809164118 37.0 35.0 37.0 32.0 37.0 9 36.369570561697905 39.0 35.0 39.0 32.0 39.0 10-11 36.21111071471428 39.0 35.0 39.0 32.0 39.0 12-13 36.0639824475486 38.0 35.0 39.0 30.5 39.0 14-15 36.99200831568467 39.0 36.0 41.0 31.0 41.0 16-17 36.84328729004031 39.0 36.0 41.0 31.0 41.0 18-19 37.04474026458407 39.0 36.0 41.0 31.0 41.0 20-21 37.051615947692916 39.0 36.0 41.0 31.0 41.0 22-23 36.9649544485994 39.0 36.0 41.0 31.0 41.0 24-25 36.81484920524238 39.0 36.0 41.0 30.0 41.0 26-27 36.60763752177029 39.0 36.0 41.0 30.0 41.0 28-29 36.39945059400064 39.0 35.0 40.5 30.0 41.0 30-31 36.10406378817707 38.5 35.0 40.0 29.0 41.0 32-33 35.79816561963851 38.0 35.0 40.0 28.5 41.0 34-35 35.516917423694515 38.0 34.5 40.0 27.0 41.0 36-37 35.21575601703353 38.0 34.0 40.0 26.5 41.0 38-39 35.05046654104491 38.0 34.0 40.0 25.0 41.0 40-41 34.90772833267928 38.0 34.0 40.0 25.0 41.0 42-43 34.56969088615231 38.0 33.5 40.0 24.0 41.0 44-45 34.13457917251802 38.0 33.0 40.0 23.5 41.0 46-47 33.62434445875077 38.0 33.0 40.0 20.0 41.0 48-49 33.18018960020497 37.5 32.0 40.0 17.0 41.0 50-51 32.4386053390328 36.5 31.5 39.5 15.0 40.5 52-53 32.45220038075717 37.0 31.0 39.0 13.5 40.0 54-55 32.55816146828266 37.0 31.0 40.0 11.5 41.0 56-57 32.240305124654185 36.5 31.0 40.0 8.5 41.0 58-59 31.88079608734712 36.0 31.0 39.0 7.5 41.0 60-61 31.415289962475633 35.5 30.0 39.0 4.0 40.5 62-63 30.857912711256986 35.0 29.0 39.0 2.0 40.0 64-65 30.349765675422354 35.0 28.5 38.0 2.0 40.0 66-67 29.826010838930635 34.5 28.0 38.0 2.0 40.0 68-69 29.257360062010875 34.0 27.5 37.0 2.0 39.5 70-71 28.639782313278175 34.0 26.0 36.5 2.0 39.0 72-73 28.01075687649392 33.5 26.0 36.0 2.0 39.0 74-75 27.346755942438854 33.0 25.0 35.5 2.0 38.5 76-77 26.616928126408787 32.5 23.0 35.0 2.0 37.5 78-79 25.851889677718567 32.0 20.5 35.0 2.0 37.0 80-81 25.157690224529972 31.0 18.5 35.0 2.0 36.0 82-83 24.431760142443398 31.0 14.5 34.0 2.0 36.0 84-85 23.72647494754048 30.5 7.0 34.0 2.0 35.0 86-87 22.965758125143918 30.0 2.0 34.0 2.0 35.0 88-89 22.1503617841749 29.0 2.0 34.0 2.0 35.0 90-91 21.234038847609565 29.0 2.0 34.0 2.0 35.0 92-93 20.28781674358567 27.0 2.0 33.5 2.0 35.0 94-95 19.277946570753052 25.5 2.0 33.0 2.0 35.0 96-97 18.02089202258597 24.0 2.0 32.5 2.0 34.0 98-99 16.497745619184453 10.5 2.0 32.0 2.0 34.0 100-101 13.041891072314673 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3454665544063822 1101 2 0.10718022095756652 1101 3 0.3137637285115673 1101 4 0.13361238319018298 1101 5 0.1292849505158813 1101 6 0.3936783334505378 1101 7 0.31495695017292036 1101 8 0.3132667865239753 1101 9 0.4162489475145321 1101 10-11 0.5388107076402378 1101 12-13 0.6297252046634725 1101 14-15 0.7750816340065327 1101 16-17 0.5402254674998872 1101 18-19 0.7127590246561653 1101 20-21 0.6513761045448234 1101 22-23 0.788903676676803 1101 24-25 0.8840828441089599 1101 26-27 0.765705849089251 1101 28-29 0.8237024961789459 1101 30-31 0.6872821372539661 1101 32-33 0.838582524444675 1101 34-35 0.6436645473321363 1101 36-37 0.8210025243470014 1101 38-39 0.7220477917442238 1101 40-41 0.5999434367606469 1101 42-43 0.657592588834639 1101 44-45 0.7714808970450022 1101 46-47 0.83099367396715 1101 48-49 0.7649684646468273 1101 50-51 0.6580602989892768 1101 52-53 0.7689610610972224 1101 54-55 0.9946377755478437 1101 56-57 0.8709992939648998 1101 58-59 1.0149823206824742 1101 60-61 1.1672602037493043 1101 62-63 1.1018679859770941 1101 64-65 0.8126028381863364 1101 66-67 0.8734077766891524 1101 68-69 0.8299432337521679 1101 70-71 1.0029310801756246 1101 72-73 1.2184859386289553 1101 74-75 0.9287538839198248 1101 76-77 0.85802770520446 1101 78-79 1.1851110496748554 1101 80-81 1.4235097429904364 1101 82-83 1.1133923549938451 1101 84-85 0.9846427336453161 1101 86-87 1.1127326566746838 1101 88-89 1.3342900790773093 1101 90-91 0.9872353064729467 1101 92-93 0.8538288991875334 1101 94-95 0.8643990653969595 1101 96-97 0.7457977597893617 1101 98-99 0.8948619474480708 1101 100-101 1.0968892396561678 1102 1 0.451366853479108 1102 2 0.4750997869263962 1102 3 0.5124116392606517 1102 4 0.348328564677729 1102 5 0.4225740992356819 1102 6 0.5421977481296523 1102 7 0.45913304739911354 1102 8 0.43442129741232804 1102 9 0.6915987423716885 1102 10-11 0.7276698729417959 1102 12-13 0.6944967421577672 1102 14-15 0.8498510674716115 1102 16-17 0.8505885628274328 1102 18-19 0.7620258706662781 1102 20-21 0.702083208649185 1102 22-23 0.7737646855087164 1102 24-25 0.7029271810141324 1102 26-27 0.7463425183108114 1102 28-29 0.7121325898082418 1102 30-31 0.6449009773327177 1102 32-33 0.6061148488458983 1102 34-35 0.6643391391755173 1102 36-37 0.9105126863071575 1102 38-39 0.8671331301673035 1102 40-41 0.8145719503642042 1102 42-43 0.8457228583006682 1102 44-45 0.8446873373782395 1102 46-47 0.7484005080544307 1102 48-49 0.9674662479017613 1102 50-51 1.0685012934937461 1102 52-53 0.840591984315175 1102 54-55 0.8212432249835189 1102 56-57 0.8710130703466703 1102 58-59 0.8936649980095517 1102 60-61 0.9089004448109428 1102 62-63 0.9353065207712277 1102 64-65 0.9646728021673603 1102 66-67 0.8144072907585951 1102 68-69 1.113449899016146 1102 70-71 1.1599805799677263 1102 72-73 1.3348400066692498 1102 74-75 1.20866992313492 1102 76-77 0.9978254178241279 1102 78-79 0.9661667713819249 1102 80-81 1.1414658789908785 1102 82-83 1.2559754015279552 1102 84-85 1.2960365528090136 1102 86-87 1.6159400488306623 1102 88-89 1.5235462546535388 1102 90-91 1.2686053801640647 1102 92-93 1.448190852327798 1102 94-95 1.5790451125773082 1102 96-97 1.771506992970906 1102 98-99 1.7296255196889128 1102 100-101 1.7700909208757007 1103 1 0.4407558167628203 1103 2 0.2944759456121808 1103 3 0.25688415824762245 1103 4 0.2503994558842635 1103 5 0.24197550871890172 1103 6 0.40783662588646763 1103 7 0.19097853858414027 1103 8 0.2833922052355149 1103 9 0.597023102386153 1103 10-11 0.5192172092270226 1103 12-13 0.6150504025459469 1103 14-15 0.8082915130585917 1103 16-17 0.9056454830121012 1103 18-19 0.7852765605832275 1103 20-21 0.7145359244946263 1103 22-23 0.8357782003871392 1103 24-25 0.8161542003448403 1103 26-27 0.6677184586993832 1103 28-29 0.6404850811659557 1103 30-31 0.7109395507767076 1103 32-33 0.7726475233777848 1103 34-35 0.7670121397196326 1103 36-37 0.7323605722452271 1103 38-39 0.763627667849633 1103 40-41 0.7100332417062774 1103 42-43 0.9092318720208112 1103 44-45 0.918446953044814 1103 46-47 0.4702544216022133 1103 48-49 0.4638606783903896 1103 50-51 0.835113467310471 1103 52-53 0.7858877607527646 1103 54-55 0.7232428437779319 1103 56-57 0.8267386479382992 1103 58-59 0.8013932896546372 1103 60-61 0.8066530423849052 1103 62-63 0.9198233019624773 1103 64-65 1.0618500888307025 1103 66-67 1.130285399457577 1103 68-69 1.3454444513602368 1103 70-71 1.2500424362760292 1103 72-73 1.5113528552524365 1103 74-75 1.441959597024944 1103 76-77 1.5029594751222568 1103 78-79 1.3369022375116408 1103 80-81 1.419080884155072 1103 82-83 1.2625552706147865 1103 84-85 1.514938566682293 1103 86-87 1.8128111492115409 1103 88-89 1.6297437777946726 1103 90-91 1.2587335401791826 1103 92-93 1.396280914591376 1103 94-95 1.585875453283876 1103 96-97 1.7807980646147499 1103 98-99 1.5946237602785125 1103 100-101 1.4218449553015002 1104 1 0.3125284779900461 1104 2 0.31156814557233403 1104 3 0.3422050414519333 1104 4 0.21681682839840732 1104 5 0.19808256751765896 1104 6 0.18499413799885644 1104 7 0.23344237149387936 1104 8 0.3935497675631012 1104 9 0.4460077835115541 1104 10-11 0.4529712163187085 1104 12-13 0.40482974725383514 1104 14-15 0.4780804310506923 1104 16-17 0.4525385797184782 1104 18-19 0.5816654805191419 1104 20-21 0.5591065278707106 1104 22-23 0.6214350394539139 1104 24-25 0.4461066740911974 1104 26-27 0.5499757694650356 1104 28-29 0.656033366316997 1104 30-31 0.6013280787101181 1104 32-33 0.6500764810236888 1104 34-35 0.45750713196866855 1104 36-37 0.6678261481082401 1104 38-39 0.7846730996436335 1104 40-41 0.6438226829083291 1104 42-43 0.7261181086834156 1104 44-45 0.7448726596617234 1104 46-47 0.848049869189694 1104 48-49 0.6013091875659953 1104 50-51 0.6229168035433261 1104 52-53 0.6743571772683836 1104 54-55 0.6539578190833879 1104 56-57 0.8560339505496515 1104 58-59 0.9258490217192978 1104 60-61 1.0402624378101386 1104 62-63 0.9955925663746434 1104 64-65 0.9602943086566356 1104 66-67 0.9587460364130465 1104 68-69 1.053607093521883 1104 70-71 1.2165559870897589 1104 72-73 1.4588909337598501 1104 74-75 1.2218311595112752 1104 76-77 1.2630944406114608 1104 78-79 1.3432281373760624 1104 80-81 1.1350724399028493 1104 82-83 1.0749836937118324 1104 84-85 1.1239851177892106 1104 86-87 1.2948658983542352 1104 88-89 1.2516298281750942 1104 90-91 1.2096532476999649 1104 92-93 1.4941021420127605 1104 94-95 1.467309072843829 1104 96-97 1.7879871394078535 1104 98-99 1.9068628639858467 1104 100-101 1.8669957871729235 1105 1 0.20368692561187274 1105 2 0.07410950799189919 1105 3 0.19255602851775677 1105 4 0.14676550766516527 1105 5 0.09240314736620547 1105 6 0.2694155716038864 1105 7 0.25199880142545084 1105 8 0.24259361182395622 1105 9 0.376709992159995 1105 10-11 0.4251986607045879 1105 12-13 0.4501883714937236 1105 14-15 0.5006544028387125 1105 16-17 0.37617837268243903 1105 18-19 0.32089318210459084 1105 20-21 0.34863981253219123 1105 22-23 0.45818770458353697 1105 24-25 0.536970783909652 1105 26-27 0.5228881182353433 1105 28-29 0.4381987867767805 1105 30-31 0.5042215930904206 1105 32-33 0.7233810495262389 1105 34-35 0.5915450152738799 1105 36-37 0.6516654418205405 1105 38-39 0.6385243460235159 1105 40-41 0.7328673652783877 1105 42-43 0.619766855575584 1105 44-45 0.837754959525661 1105 46-47 0.6323240185569006 1105 48-49 0.6796168451545626 1105 50-51 0.6447878016199056 1105 52-53 0.6520154155551339 1105 54-55 0.7113592653215761 1105 56-57 0.7049847569990888 1105 58-59 0.6969419814865994 1105 60-61 0.7703851208514685 1105 62-63 0.7662068812030647 1105 64-65 1.1416188518390555 1105 66-67 1.104692759579038 1105 68-69 0.8940951114435833 1105 70-71 1.0804195629019269 1105 72-73 1.2111533480602965 1105 74-75 1.1326140496270867 1105 76-77 1.4036215190357986 1105 78-79 1.3086642059826055 1105 80-81 1.2537681189885248 1105 82-83 1.3927956673705992 1105 84-85 1.3083554959340944 1105 86-87 1.6978853213429552 1105 88-89 1.5605326624835918 1105 90-91 1.6841718443053146 1105 92-93 1.5918783460931216 1105 94-95 1.633629154440296 1105 96-97 1.903728099073657 1105 98-99 2.050201452802167 1105 100-101 1.765892520200154 1106 1 0.22282255599753853 1106 2 0.13822818680161575 1106 3 -0.03729735538598433 1106 4 0.02251611089010197 1106 5 0.13137191357214562 1106 6 -0.19554934319189243 1106 7 0.12042748887077437 1106 8 0.1528748948859615 1106 9 0.01030441090610168 1106 10-11 0.05572409336410544 1106 12-13 0.04473761617174432 1106 14-15 0.40306454132984726 1106 16-17 0.31981868531919844 1106 18-19 0.10013857503201962 1106 20-21 0.1860578773933952 1106 22-23 0.19023157328759055 1106 24-25 0.046517794981610905 1106 26-27 -0.0067187935744783545 1106 28-29 0.10394691998518368 1106 30-31 -0.20808219095221858 1106 32-33 0.09029538609985366 1106 34-35 -0.2853049911754013 1106 36-37 -0.1957781288928615 1106 38-39 -0.1858903867740267 1106 40-41 0.07390459065926791 1106 42-43 -0.38820604005922377 1106 44-45 -0.15539575649544446 1106 46-47 0.05338940136974912 1106 48-49 0.21688459078597333 1106 50-51 -0.10822189272217742 1106 52-53 -0.03569184549913729 1106 54-55 0.04595440010768925 1106 56-57 -0.7947935845493461 1106 58-59 -0.5098852122984887 1106 60-61 0.1415243961191095 1106 62-63 0.16190901460892348 1106 64-65 0.12681350465043195 1106 66-67 -0.5219710499883625 1106 68-69 -0.23992673742242232 1106 70-71 -0.67700495501931 1106 72-73 -0.19192800702179014 1106 74-75 0.3358339979799112 1106 76-77 0.23157673495042275 1106 78-79 0.3811542739061693 1106 80-81 0.32293425578053814 1106 82-83 0.041627780762890865 1106 84-85 0.25221916917776355 1106 86-87 0.03155183582432741 1106 88-89 0.03411332024937508 1106 90-91 0.4033097820571143 1106 92-93 0.26355508636729397 1106 94-95 0.07505817815586013 1106 96-97 0.18312025561747802 1106 98-99 0.2878089108147215 1106 100-101 -0.24977676539011817 1107 1 0.17987198983483665 1107 2 -0.02764630517330957 1107 3 0.14875042411207318 1107 4 0.12455764139886583 1107 5 0.060644582642780165 1107 6 0.22909128651055255 1107 7 0.27553459361501353 1107 8 0.15254189050496336 1107 9 0.3007590097739552 1107 10-11 0.36935399214771536 1107 12-13 0.46473773404940744 1107 14-15 0.6481279539837956 1107 16-17 0.6453625157315628 1107 18-19 0.5609726183940609 1107 20-21 0.3455492942423106 1107 22-23 0.21481238007910974 1107 24-25 0.3372914002586924 1107 26-27 0.3075049139062216 1107 28-29 0.43553695328194664 1107 30-31 0.436591083493866 1107 32-33 0.3666315569694092 1107 34-35 0.254459194571794 1107 36-37 0.3969409366025545 1107 38-39 0.5566276052242927 1107 40-41 0.5985354954550104 1107 42-43 0.541514932475657 1107 44-45 0.5491424227883712 1107 46-47 0.46042739739064587 1107 48-49 0.6822228499319039 1107 50-51 0.5865265471486154 1107 52-53 0.754829980469971 1107 54-55 0.8179023910119554 1107 56-57 0.8203069485155225 1107 58-59 0.6305755935647461 1107 60-61 0.8053159530582512 1107 62-63 0.8750150577175191 1107 64-65 0.6893304725866045 1107 66-67 0.9253788945170491 1107 68-69 1.0564151136767315 1107 70-71 1.101648432729295 1107 72-73 1.0493198532224604 1107 74-75 0.9322866540766341 1107 76-77 1.1642678560737671 1107 78-79 0.9647553184495585 1107 80-81 0.9145647947871751 1107 82-83 0.8277352726615668 1107 84-85 1.0007113481794043 1107 86-87 1.2740775635438837 1107 88-89 1.290114306001282 1107 90-91 1.1707649765755086 1107 92-93 1.3278982370676147 1107 94-95 1.6136305102805792 1107 96-97 1.6528477998317328 1107 98-99 1.3799816870435144 1107 100-101 1.3505188865083202 1108 1 0.21490076572912287 1108 2 0.13024475139616243 1108 3 0.004734093778044013 1108 4 0.0013737796817068215 1108 5 0.016662018318157834 1108 6 0.036869210121594165 1108 7 0.18985942112978904 1108 8 0.2799220108884555 1108 9 0.20455018850682904 1108 10-11 0.2498071746221484 1108 12-13 0.20381659880849412 1108 14-15 0.34093560701430903 1108 16-17 0.28697353205105713 1108 18-19 0.29642546993915886 1108 20-21 0.3248407838991838 1108 22-23 0.3598272943347638 1108 24-25 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2316 58-59 -1.3395186873020357 2316 60-61 -1.3527758380672203 2316 62-63 -1.3929661456398676 2316 64-65 -1.4809025751875673 2316 66-67 -1.6195436420500862 2316 68-69 -1.5736866801200904 2316 70-71 -1.992649954176052 2316 72-73 -1.6551105901948908 2316 74-75 -1.8004815516857136 2316 76-77 -1.9069855410403456 2316 78-79 -2.2397372931451436 2316 80-81 -2.478195476055081 2316 82-83 -2.7001484736003327 2316 84-85 -2.851392065004241 2316 86-87 -2.845657592485054 2316 88-89 -2.9728582145339963 2316 90-91 -2.6201033470803132 2316 92-93 -2.752038924092272 2316 94-95 -3.628710464463694 2316 96-97 -3.4229659156738794 2316 98-99 -3.378017379303497 2316 100-101 -3.0914808414588055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4811.0 3 1534.0 4 1689.0 5 1904.0 6 2356.0 7 2966.0 8 3727.0 9 4838.0 10 6080.0 11 8013.0 12 10373.0 13 12745.0 14 14911.0 15 17874.0 16 21845.0 17 25971.0 18 23246.0 19 19328.0 20 20043.0 21 21588.0 22 23589.0 23 26906.0 24 29893.0 25 33263.0 26 37662.0 27 42974.0 28 49065.0 29 55936.0 30 64241.0 31 74878.0 32 87959.0 33 104249.0 34 126403.0 35 154037.0 36 173632.0 37 162008.0 38 66310.0 39 2818.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.68953384506305 18.263146184179885 14.607065917905718 22.44025405285135 2 15.71316606786764 20.363798816514254 39.774367592256496 24.148667523361603 3 19.185269125492322 25.03423934748798 29.543767594543784 26.236723932475915 4 12.85505358355879 16.715979213397773 36.063923607548325 34.36504359549511 5 13.950449970504389 37.43214060181443 32.83108499673859 15.786324430942585 6 31.99171029668412 35.525611859054266 17.284044398736807 15.198633445524806 7 29.11213034087245 33.39832382914918 19.59661340041304 17.892932429565334 8 27.54282073458509 33.586742198383 19.294780668431034 19.575656398600877 9 27.8541449454393 16.458326710183734 19.57554315774039 36.111985186636566 10-11 25.54860724432832 26.648547409244717 26.097798562140877 21.705046784286086 12-13 26.85852058208415 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29.64755472125461 22.309268582349993 38-39 21.903979267941352 26.00623674723655 29.722460854872995 22.367323129949103 40-41 22.219132829434262 25.832619294270938 29.75961243657597 22.188635439718826 42-43 22.211289026965087 25.897560177993718 29.400173114332024 22.49097768070917 44-45 22.024298746530302 26.239149796861756 29.31791216825023 22.41863928835771 46-47 22.267434977156654 26.573595787158748 28.664446934840115 22.494522300844483 48-49 22.522881354282962 26.490377473421038 28.395681199113355 22.591059973182645 50-51 22.612812365799957 26.75590415311422 27.989312000581236 22.641971480504587 52-53 22.87705823301314 26.767562588285283 27.89323542147455 22.462143757227025 54-55 22.213266351347933 26.846388830684738 28.12286532222647 22.817479495740862 56-57 22.28475693419529 27.14292665046985 28.058457755821607 22.513858659513254 58-59 22.397023427210858 26.393468148465328 28.189305617729683 23.020202806594135 60-61 22.242699237556483 26.698823618717903 28.457507634005054 22.60096950972056 62-63 22.155880323799497 26.86363593607435 28.317859863983973 22.662623876142185 64-65 21.983441158898316 26.628427675567114 28.264591156984636 23.12354000854993 66-67 22.021450229059333 26.971622850663174 28.016434407034087 22.990492513243403 68-69 22.18603794246094 26.531438372693465 28.256422997978582 23.02610068686701 70-71 22.03412704875353 26.908928463095428 27.923909210384167 23.133035277766872 72-73 22.343551957188122 26.758462019088995 27.887412494172764 23.010573529550115 74-75 22.096147077095765 27.10914803574412 27.727581659483562 23.06712322767655 76-77 22.170711673859618 26.817301229332703 27.82808805935676 23.18389903745092 78-79 22.388149741486703 26.947785211956255 27.662365841800433 23.001699204756605 80-81 22.270487278501662 27.246573858509613 27.490248786490422 22.992690076498295 82-83 22.376003840215883 27.057726488407997 27.377106604911845 23.18916306646428 84-85 22.2838288495457 26.807337354719895 27.45485021643224 23.45398357930216 86-87 22.57742662792889 26.724427342503983 27.356516532374336 23.341629497192788 88-89 22.434025444721335 26.75095897068695 27.33300867461702 23.48200690997469 90-91 22.36662044568104 26.74861645255125 27.467431724553204 23.417331377214502 92-93 22.587288378905775 26.375405318163608 27.495616318810818 23.541689984119802 94-95 22.47452485053984 26.226270918338372 27.492752094875765 23.806452136246016 96-97 22.839279051280805 26.084099868593956 27.290245129053716 23.786375951071527 98-99 22.6906605842743 26.134357850084942 26.971000392855416 24.203981172785337 100-101 23.250221675804166 25.536241235204844 27.363005651272477 23.850531437718512 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1311.0 1 887.0 2 582.0 3 749.0 4 652.5 5 627.5 6 1094.0 7 2391.5 8 3813.5 9 4135.5 10 3405.0 11 2682.5 12 2601.0 13 2778.5 14 2983.0 15 3069.0 16 3155.0 17 3261.0 18 3414.0 19 3687.5 20 3969.5 21 4420.0 22 5055.0 23 5698.5 24 6419.5 25 7460.0 26 8856.5 27 10553.5 28 12093.5 29 13645.5 30 15500.5 31 17859.5 32 21055.5 33 23099.5 34 24578.5 35 26525.0 36 28712.5 37 31340.0 38 33597.5 39 34867.0 40 36606.5 41 40655.0 42 45193.0 43 49117.5 44 52002.5 45 54285.5 46 55316.5 47 56598.0 48 60280.0 49 61809.5 50 60886.5 51 59455.0 52 57370.5 53 56783.0 54 56229.5 55 52405.0 56 46799.0 57 41930.5 58 38606.0 59 34884.0 60 29545.0 61 24231.0 62 19652.5 63 15290.5 64 11870.5 65 9550.5 66 7357.0 67 5708.0 68 4322.0 69 3098.5 70 2468.5 71 2041.0 72 1783.5 73 1536.5 74 1156.5 75 987.5 76 760.5 77 463.0 78 273.0 79 168.5 80 99.0 81 55.0 82 31.5 83 21.5 84 14.5 85 13.0 86 9.0 87 3.0 88 1.0 89 1.0 90 2.0 91 1.0 92 0.5 93 1.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006227033758955416 2 0.10624876351217677 3 0.11481093493074046 4 0.11850823622512024 5 0.15911368552830868 6 0.09347037131932034 7 0.026854083085495226 8 0.026854083085495226 9 0.022962186986148093 10-11 0.04949194539669773 12-13 0.0533514090285503 14-15 0.026983812955473467 16-17 0.031102736327282512 18-19 0.035837876581488196 20-21 0.05756762980284303 22-23 0.030810844119831482 24-25 0.04709194280210033 26-27 0.05614060123308241 28-29 0.051600055783844086 30-31 0.06632439602637408 32-33 0.04923248565674125 34-35 0.061394660967201045 36-37 0.05108113630393114 38-39 0.030940573989809716 40-41 0.03564328177652084 42-43 0.031589223339700906 44-45 0.006745953238868367 46-47 0.012162175310459796 48-49 0.008172981808628983 50-51 0.007978387003661627 52-53 0.009243253235949444 54-55 0.01011892985830255 56-57 0.01362163634771497 58-59 0.019135155821790078 60-61 0.024032458413468554 62-63 0.01008649739080799 64-65 0.008562171418563694 66-67 0.009275685703444005 68-69 0.011221633753117572 70-71 0.029416248017565424 72-73 0.02662705581303331 74-75 0.02805408438279393 76-77 0.02211894283128955 78-79 0.003989193501830813 80-81 0.004021625969325372 82-83 0.005513519474075107 84-85 0.004800005189194799 86-87 0.012291905180438031 88-89 0.036291931126412026 90-91 0.03360003632436359 92-93 0.028378409057739522 94-95 0.045567616829856036 96-97 0.0415135583930361 98-99 0.025005432438305338 100-101 0.073102781732737 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1541665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.760511108213834 #Duplication Level Percentage of deduplicated Percentage of total 1 75.89989078506004 37.00917467737137 2 12.58841409435749 12.276350105654279 3 4.553737909897128 6.661277638183001 4 2.1177148096293346 4.130434259958404 5 1.22455100999986 2.985486656283632 6 0.7582530058524495 2.218368246882293 7 0.5005499765207062 1.7084950883247845 8 0.3525264304402818 1.3751495141937864 9 0.26866332903081513 1.179014511561909 >10 1.496198174076993 13.57259625285446 >50 0.14510203918595974 4.886292197401578 >100 0.08784677905729657 7.958968240751989 >500 0.00481346220612016 1.566798799157668 >1k 0.0013370728350333779 0.892733686620587 >5k 2.6741456700667555E-4 0.89175574129821 >10k+ 1.3370728350333778E-4 0.6871043835021082 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10539 0.6836115498503241 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7846 0.5089302799246269 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5832 0.3782923008565415 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1894 0.1228541868693912 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1707 0.11072444402642598 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1590 0.10313524663269906 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.48649349891189E-5 0.0 8 0.0 0.0 0.0 6.48649349891189E-5 0.0 9 0.0 0.0 0.0 6.48649349891189E-5 0.0 10-11 0.0 0.0 0.0 9.729740248367836E-5 0.0 12-13 0.0 0.0 0.0 1.297298699782378E-4 0.0 14-15 0.0 0.0 0.0 1.297298699782378E-4 0.0 16-17 0.0 0.0 0.0 1.9459480496735672E-4 0.0 18-19 0.0 0.0 0.0 1.9459480496735672E-4 0.0 20-21 0.0 0.0 0.0 4.216220774292729E-4 0.0 22-23 0.0 0.0 0.0 9.729740248367836E-4 0.0 24-25 0.0 0.0 0.0 0.001848650647189889 6.48649349891189E-5 26-27 0.0 0.0 0.0 0.003632436359390659 6.48649349891189E-5 28-29 0.0 0.0 0.0 0.007264872718781318 6.48649349891189E-5 30-31 0.0 0.0 0.0 0.019135155821790078 6.48649349891189E-5 32-33 0.0 0.0 6.48649349891189E-5 0.04102707138061771 6.48649349891189E-5 34-35 0.0 0.0 6.48649349891189E-5 0.07504872978241058 6.48649349891189E-5 36-37 0.0 0.0 6.48649349891189E-5 0.13002176218568884 6.48649349891189E-5 38-39 0.0 0.0 6.48649349891189E-5 0.21447590754152168 6.48649349891189E-5 40-41 0.0 0.0 6.48649349891189E-5 0.30745979184842365 6.48649349891189E-5 42-43 0.0 0.0 6.48649349891189E-5 0.4042707073196836 6.48649349891189E-5 44-45 0.0 0.0 6.48649349891189E-5 0.5207681305601412 6.48649349891189E-5 46-47 0.0 0.0 6.48649349891189E-5 0.6613628771490564 6.48649349891189E-5 48-49 0.0 0.0 6.48649349891189E-5 0.8178171003428112 6.48649349891189E-5 50-51 0.0 0.0 6.48649349891189E-5 0.9871794455994007 6.48649349891189E-5 52-53 0.0 0.0 6.48649349891189E-5 1.1533634090415235 6.48649349891189E-5 54-55 0.0 0.0 6.48649349891189E-5 1.3306717088342799 6.48649349891189E-5 56-57 0.0 0.0 6.48649349891189E-5 1.53973139430421 6.48649349891189E-5 58-59 0.0 0.0 6.48649349891189E-5 1.768769479750789 6.48649349891189E-5 60-61 0.0 0.0 6.48649349891189E-5 2.0171048833566307 6.48649349891189E-5 62-63 0.0 0.0 6.48649349891189E-5 2.262521364887962 6.48649349891189E-5 64-65 0.0 0.0 6.48649349891189E-5 2.5213973204295357 6.48649349891189E-5 66-67 0.0 0.0 6.48649349891189E-5 2.8180895330697657 6.48649349891189E-5 68-69 0.0 0.0 6.48649349891189E-5 3.1420898833404145 6.48649349891189E-5 70-71 0.0 0.0 6.48649349891189E-5 3.489214582934684 6.48649349891189E-5 72-73 0.0 0.0 6.48649349891189E-5 3.842728478625382 6.48649349891189E-5 74-75 0.0 0.0 6.48649349891189E-5 4.193615344449022 6.48649349891189E-5 76-77 0.0 0.0 6.48649349891189E-5 4.584069820616023 6.48649349891189E-5 78-79 0.0 3.243246749455945E-5 6.48649349891189E-5 5.015745962968609 6.48649349891189E-5 80-81 0.0 6.48649349891189E-5 6.48649349891189E-5 5.470416724774838 6.48649349891189E-5 82-83 0.0 6.48649349891189E-5 6.48649349891189E-5 5.944060480065383 6.48649349891189E-5 84-85 0.0 6.48649349891189E-5 6.48649349891189E-5 6.412060986011877 6.48649349891189E-5 86-87 0.0 6.48649349891189E-5 6.48649349891189E-5 6.885964201042379 6.48649349891189E-5 88-89 0.0 1.297298699782378E-4 6.48649349891189E-5 7.401672866673369 6.48649349891189E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4545 0.0 55.14011 1 GTATCAA 8595 0.0 44.70503 1 TATCAAC 11360 0.0 33.614616 2 ATCAACG 11445 0.0 33.323414 3 TCAACGC 11580 0.0 32.81173 4 CAACGCA 11730 0.0 32.31106 5 AACGCAG 12150 0.0 31.36741 6 ACGCAGA 13750 0.0 27.584517 7 CGCAGAG 13870 0.0 27.277327 8 GTACATG 9030 0.0 25.541412 1 GCAGAGT 14445 0.0 25.138596 9 TACATGG 9290 0.0 24.263504 2 GAGTACT 7955 0.0 24.136715 12-13 AGTACTT 8190 0.0 23.183014 12-13 GTACTTT 8470 0.0 22.689823 14-15 ACATGGG 9590 0.0 22.51273 3 AGAGTAC 13090 0.0 21.821922 10-11 TAAGGTG 635 0.0 21.71777 5 GTAAGGT 505 0.0 21.65845 3 CAGAGTA 14040 0.0 21.208605 10-11 >>END_MODULE