FastQCFastQC Report
Thu 26 May 2016
SRR938651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938651_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541665
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102100.662270986238904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83060.5387681500196216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60570.39288691122909325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20000.12972986997823782No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19410.12590283881387979No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG17770.11526498947566431No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47150.052.4994931
GTATCAA86350.043.1372871
ATCAACG115850.032.1392633
TATCAAC115850.032.1118132
ATCCGCG600.003962373431.6486767
TCAACGC118300.031.5138024
CAACGCA120650.030.899985
AACGCAG124350.030.007076
ACGCAGA143150.025.966737
CGCAGAG143950.025.6575228
TTGTACG1301.006825E-525.5623939
GTACATG93300.024.9524691
GTATAGA3100.024.5220831
GCAGAGT149750.024.0297459
TACATGG96450.023.8912352
AGTACTT83600.022.7995412-13
GAGTACT82050.022.73844712-13
ACATGGG98450.021.997083
AGAGTAC136450.021.6751110-11
TGGTATC21650.021.506382
Produced by FastQC (version 0.11.5)