##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938651_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1541665 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.729399058809793 31.0 31.0 34.0 30.0 34.0 2 31.94890199881297 31.0 31.0 34.0 30.0 34.0 3 31.992951127514733 33.0 31.0 34.0 30.0 34.0 4 35.696011779472194 37.0 35.0 37.0 33.0 37.0 5 35.595557400602594 37.0 35.0 37.0 33.0 37.0 6 35.60403330165762 37.0 35.0 37.0 33.0 37.0 7 35.54182588305501 37.0 35.0 37.0 33.0 37.0 8 35.52459451307515 37.0 35.0 37.0 33.0 37.0 9 37.07611835256038 39.0 37.0 39.0 33.0 39.0 10-11 36.975832946846424 39.0 37.0 39.0 33.0 39.0 12-13 36.85187605608222 39.0 37.0 39.0 32.0 39.0 14-15 37.827408029630305 40.0 37.0 41.0 32.5 41.0 16-17 37.756275844622536 39.0 37.0 41.0 32.0 41.0 18-19 38.03404890167449 40.0 37.0 41.0 33.0 41.0 20-21 38.02230607816873 40.0 37.0 41.0 33.0 41.0 22-23 37.90726260244605 40.0 37.0 41.0 32.5 41.0 24-25 37.76387347445781 40.0 37.0 41.0 32.0 41.0 26-27 37.556260601362816 39.5 36.5 41.0 32.0 41.0 28-29 37.419269750561895 39.0 36.0 41.0 32.0 41.0 30-31 37.1193835885228 39.0 36.0 40.5 31.0 41.0 32-33 36.872839105772 39.0 35.0 40.0 31.0 41.0 34-35 36.71944942643181 39.0 35.0 40.0 30.0 41.0 36-37 36.63058511414607 39.0 35.0 40.0 30.0 41.0 38-39 36.45486081606575 39.0 35.0 40.0 30.0 41.0 40-41 36.24500848109025 39.0 35.0 40.0 30.0 41.0 42-43 35.9718216992667 38.0 35.0 40.0 29.5 41.0 44-45 35.57296397077186 38.0 35.0 40.0 27.5 41.0 46-47 35.05271054347086 38.0 34.0 40.0 26.5 41.0 48-49 34.59716734828902 38.0 33.5 40.0 24.0 41.0 50-51 34.17203769950022 38.0 33.0 40.0 23.5 41.0 52-53 33.66122244456481 37.0 33.0 40.0 22.5 41.0 54-55 33.3026549217891 37.0 32.0 40.0 21.0 41.0 56-57 32.94904080977385 36.5 32.0 39.5 19.5 41.0 58-59 32.458474765918666 36.0 31.0 39.0 18.0 40.0 60-61 32.055315194935346 35.5 31.0 39.0 15.0 40.0 62-63 31.570534778956517 35.0 30.0 38.5 12.5 40.0 64-65 31.033803712220227 35.0 29.5 38.0 8.0 40.0 66-67 30.516460450227513 34.0 29.0 38.0 7.5 40.0 68-69 30.018340560368173 34.0 28.0 37.0 6.0 39.0 70-71 29.442645451508596 34.0 28.0 36.5 2.0 39.0 72-73 28.93848339295502 33.5 26.5 36.0 2.0 39.0 74-75 28.339240042421668 33.0 26.0 35.5 2.0 38.0 76-77 27.153500274054352 31.5 25.5 34.0 2.0 36.0 78-79 27.83826966299423 33.0 26.0 35.0 2.0 37.0 80-81 27.448004268112722 33.0 26.0 35.0 2.0 36.0 82-83 26.995347886862582 32.5 25.5 35.0 2.0 36.0 84-85 26.43997139456367 32.0 24.5 35.0 2.0 35.5 86-87 25.81182098575242 32.0 23.0 34.0 2.0 35.0 88-89 25.04738772690565 31.0 19.5 34.0 2.0 35.0 90-91 24.41438606960656 31.0 17.5 34.0 2.0 35.0 92-93 23.564514988664854 30.0 8.5 34.0 2.0 35.0 94-95 22.720201211028336 30.0 2.0 34.0 2.0 35.0 96-97 21.62584770361914 29.0 2.0 34.0 2.0 35.0 98-99 20.242625667703425 29.0 2.0 34.0 2.0 35.0 100-101 16.479707005088656 20.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3155900873183377 1101 2 0.3573452374005761 1101 3 0.38083400421483304 1101 4 0.22259316893738657 1101 5 0.061059078637768494 1101 6 0.14647504316464932 1101 7 0.2673294852173598 1101 8 0.2508696490295179 1101 9 0.29085422869169975 1101 10-11 0.22196784740695108 1101 12-13 0.3206028870060109 1101 14-15 0.40255439495390277 1101 16-17 0.4020416840626666 1101 18-19 0.33168072252708924 1101 20-21 0.4327749655070221 1101 22-23 0.4286559765864908 1101 24-25 0.4767117121252582 1101 26-27 0.28885988871774515 1101 28-29 0.21065972281197531 1101 30-31 0.3183429985650079 1101 32-33 0.2704706278869651 1101 34-35 0.4804190199394469 1101 36-37 0.606744522100982 1101 38-39 0.6292184127500988 1101 40-41 0.5297527363165742 1101 42-43 0.3599010659508153 1101 44-45 0.4506335337280589 1101 46-47 0.4680158762862803 1101 48-49 0.5809331730376712 1101 50-51 0.5568703726748652 1101 52-53 0.4791389079176511 1101 54-55 0.6471258369257171 1101 56-57 0.7498241662197245 1101 58-59 0.8235329517634611 1101 60-61 0.698392557354282 1101 62-63 0.6132100729097338 1101 64-65 0.5222518756315822 1101 66-67 0.5961662148496494 1101 68-69 0.547050953943053 1101 70-71 0.6635826263720368 1101 72-73 0.6504194495383793 1101 74-75 0.6599308630666485 1101 76-77 0.553491347570013 1101 78-79 0.6487560777779144 1101 80-81 0.7198088788169166 1101 82-83 0.7285017132295373 1101 84-85 1.0210715807501423 1101 86-87 0.8747976926898424 1101 88-89 0.7285372491363376 1101 90-91 0.7908529578918575 1101 92-93 1.05136431002256 1101 94-95 1.218587584284272 1101 96-97 1.2438623856863913 1101 98-99 1.2610274926000322 1101 100-101 1.360945881686277 1102 1 0.3150290128607516 1102 2 0.3107434950640062 1102 3 0.366681902940897 1102 4 0.2497301362297506 1102 5 0.15451554777617105 1102 6 0.18816237440397288 1102 7 0.16683704218895912 1102 8 0.28052393699096 1102 9 0.38617677136090833 1102 10-11 0.46663388286484775 1102 12-13 0.49807148937956214 1102 14-15 0.57572601861321 1102 16-17 0.5197708854833891 1102 18-19 0.4894278081961474 1102 20-21 0.4095091614923305 1102 22-23 0.5021830308954947 1102 24-25 0.402409423242041 1102 26-27 0.44321422727607995 1102 28-29 0.3839027331768321 1102 30-31 0.4655436377891178 1102 32-33 0.6348534209356558 1102 34-35 0.6820852106947441 1102 36-37 0.6858560206206405 1102 38-39 0.7426994789919519 1102 40-41 0.586447556897987 1102 42-43 0.631155518226926 1102 44-45 0.8216928623423598 1102 46-47 1.0551427242000315 1102 48-49 1.0340546506673292 1102 50-51 1.1826762910084767 1102 52-53 0.9651158036730223 1102 54-55 0.8033437442181821 1102 56-57 0.966185999129749 1102 58-59 1.1572106946410727 1102 60-61 0.8544489052605257 1102 62-63 0.9475979256477238 1102 64-65 0.8801221972975704 1102 66-67 0.9054585095940872 1102 68-69 0.9862157044402551 1102 70-71 1.2453842765320928 1102 72-73 1.2587005578608164 1102 74-75 1.3127134417047408 1102 76-77 1.1617536699173066 1102 78-79 1.4439125524268697 1102 80-81 1.4617409286924286 1102 82-83 1.4190541210905927 1102 84-85 1.4986711839903464 1102 86-87 1.503143474862707 1102 88-89 1.396724327391194 1102 90-91 1.3178496565698268 1102 92-93 1.8723289573221393 1102 94-95 2.1026994084275046 1102 96-97 2.1427324719014926 1102 98-99 2.266432843847671 1102 100-101 2.316246532432455 1103 1 0.3208423980641122 1103 2 0.27203646621944344 1103 3 0.27919929340945515 1103 4 0.19306490315674552 1103 5 0.10216901560665548 1103 6 0.12807036375627945 1103 7 0.23534652834337777 1103 8 0.22177999310963514 1103 9 0.3983940846108993 1103 10-11 0.37907104096090904 1103 12-13 0.43941958872759557 1103 14-15 0.5357812934233337 1103 16-17 0.5802819779143391 1103 18-19 0.5736595811931053 1103 20-21 0.5322382188067536 1103 22-23 0.42417843036311353 1103 24-25 0.49308422229456284 1103 26-27 0.5052770219365996 1103 28-29 0.452533586067311 1103 30-31 0.3681688247264745 1103 32-33 0.4825084363956549 1103 34-35 0.6447368376606377 1103 36-37 0.6903974987208699 1103 38-39 0.7710540881584791 1103 40-41 0.7191555664509295 1103 42-43 0.7126378051997904 1103 44-45 0.6655350073894581 1103 46-47 0.6628581460838419 1103 48-49 0.5946647099333973 1103 50-51 0.8209859048830523 1103 52-53 0.7903012748529008 1103 54-55 0.8990801063439378 1103 56-57 1.0179252903480105 1103 58-59 1.1524281121524211 1103 60-61 0.9221475150004892 1103 62-63 0.7966637275967621 1103 64-65 1.0668677279064234 1103 66-67 0.8587669342375612 1103 68-69 0.9154857771951299 1103 70-71 0.6610885619996871 1103 72-73 0.7397078854793229 1103 74-75 0.7835968286349271 1103 76-77 0.6744193884572596 1103 78-79 0.9570226035475144 1103 80-81 0.8407623218196498 1103 82-83 0.9897087032143297 1103 84-85 1.1103473156333443 1103 86-87 1.1255059883433738 1103 88-89 1.3185026514550415 1103 90-91 1.4496022085294484 1103 92-93 1.6896068561951374 1103 94-95 1.6200430408280226 1103 96-97 1.8430788150973 1103 98-99 2.0627212209870684 1103 100-101 2.3024909568934753 1104 1 0.21961139682169062 1104 2 0.18575218017425144 1104 3 0.14978055860562023 1104 4 0.04587322851234177 1104 5 0.026746195553620566 1104 6 0.05573780308903764 1104 7 0.12374811384771078 1104 8 0.11719834239034554 1104 9 0.2159845489070804 1104 10-11 0.19316851189683604 1104 12-13 0.2336320443640929 1104 14-15 0.33683577205191284 1104 16-17 0.37388678917810125 1104 18-19 0.3018216402105409 1104 20-21 0.2211836267890277 1104 22-23 0.24085165740221015 1104 24-25 0.3277600022094589 1104 26-27 0.19335378801315528 1104 28-29 0.2825936175247037 1104 30-31 0.29250808993236177 1104 32-33 0.45780235727918495 1104 34-35 0.4675731701370722 1104 36-37 0.4210883383351529 1104 38-39 0.3767981801786391 1104 40-41 0.4370678534615564 1104 42-43 0.3564640492812856 1104 44-45 0.289881399913547 1104 46-47 0.3050726443449392 1104 48-49 0.2494615570670078 1104 50-51 0.5827052813075042 1104 52-53 0.3195467672290988 1104 54-55 0.20453833620967288 1104 56-57 0.0889431442801012 1104 58-59 0.2803697507552769 1104 60-61 0.4416474121403269 1104 62-63 0.3795072602844236 1104 64-65 0.3642350342498979 1104 66-67 0.5004023378948474 1104 68-69 0.5498721717926287 1104 70-71 0.6799943809690454 1104 72-73 0.45572677611224677 1104 74-75 0.5282071992068786 1104 76-77 0.4314772558016706 1104 78-79 0.6735169187013277 1104 80-81 0.43856066892976386 1104 82-83 0.6469298509393404 1104 84-85 0.7820986758982222 1104 86-87 0.7775444418449133 1104 88-89 0.873092441895686 1104 90-91 0.8992908338155559 1104 92-93 1.3895524294016077 1104 94-95 1.3153252826287734 1104 96-97 1.152781443943823 1104 98-99 1.518846705523213 1104 100-101 1.81945822630448 1105 1 0.07042911075963687 1105 2 0.13441414650916883 1105 3 0.12699859733011465 1105 4 0.144938890524422 1105 5 -0.002827525954991472 1105 6 0.141479066473309 1105 7 0.20877929112587879 1105 8 0.1805943839774784 1105 9 0.27040167278538974 1105 10-11 0.1722225095816583 1105 12-13 0.1905947519643192 1105 14-15 0.3145743611757936 1105 16-17 0.3479790265567715 1105 18-19 0.27828345837698265 1105 20-21 0.25865633537729593 1105 22-23 0.2426951132109778 1105 24-25 0.21665344046791546 1105 26-27 0.14565883823626535 1105 28-29 0.1872427400282035 1105 30-31 0.38859615752549104 1105 32-33 0.39790119265339285 1105 34-35 0.5574653808346497 1105 36-37 0.5252658898490736 1105 38-39 0.4016848335970238 1105 40-41 0.48713219179932565 1105 42-43 0.6886053837126127 1105 44-45 0.5684170001400659 1105 46-47 0.718451366289905 1105 48-49 0.8895828445411524 1105 50-51 1.0155761936508867 1105 52-53 0.9102651690701791 1105 54-55 1.069694830037463 1105 56-57 0.9942689054120564 1105 58-59 1.1458662939392283 1105 60-61 1.2382144706807168 1105 62-63 1.2362459466751083 1105 64-65 1.242132325888452 1105 66-67 1.1956237313629003 1105 68-69 1.2602575751595957 1105 70-71 1.276487278377946 1105 72-73 1.453434278304595 1105 74-75 1.3220215687815156 1105 76-77 1.1867778159892524 1105 78-79 1.4312564535814722 1105 80-81 1.5345487785436838 1105 82-83 1.511994652543894 1105 84-85 1.4694030129595994 1105 86-87 1.3414550747980591 1105 88-89 1.4244016415283056 1105 90-91 1.4793692854510816 1105 92-93 1.3477782598505748 1105 94-95 1.270937019694692 1105 96-97 1.360670973460838 1105 98-99 1.5447481246573993 1105 100-101 1.2777009727191633 1106 1 -3.234348136800236E-4 1106 2 -0.004930720376606246 1106 3 0.07744961175796305 1106 4 0.09403461104110988 1106 5 -0.11227157554142053 1106 6 -0.1266769735114508 1106 7 0.018157940121398042 1106 8 0.0747017869536748 1106 9 -0.09176818945728371 1106 10-11 0.1442439930516528 1106 12-13 0.02670764193283759 1106 14-15 0.02701356113252018 1106 16-17 0.09553501517426355 1106 18-19 0.05086718267018142 1106 20-21 0.1838053779905664 1106 22-23 0.1683226506045017 1106 24-25 0.19986054322173885 1106 26-27 0.05752861049166569 1106 28-29 0.054825456267181494 1106 30-31 -0.07063873499475193 1106 32-33 9.895833109041519E-4 1106 34-35 0.0027043955968863997 1106 36-37 0.07801549485446913 1106 38-39 -0.05956597823650611 1106 40-41 0.008380764352942549 1106 42-43 -0.004081627366900875 1106 44-45 -0.21708702886163422 1106 46-47 -0.7113477755968489 1106 48-49 -0.6354861211195697 1106 50-51 -0.36119570278090407 1106 52-53 -0.5020008271238012 1106 54-55 -0.08576188282822272 1106 56-57 -0.31641056852359384 1106 58-59 -0.481084423367939 1106 60-61 -0.34738748899181715 1106 62-63 -0.5426270524885659 1106 64-65 -0.38580412849170287 1106 66-67 -0.3728252306438584 1106 68-69 -0.4752915987844162 1106 70-71 -0.3735853280039301 1106 72-73 -0.09410502919052632 1106 74-75 -0.0319100507209491 1106 76-77 -0.053043011356589176 1106 78-79 0.37932445052667063 1106 80-81 -0.2550771141895112 1106 82-83 0.2835824268320444 1106 84-85 0.5207818155273607 1106 86-87 0.6536292302547686 1106 88-89 0.6480756341454885 1106 90-91 0.5349847526719174 1106 92-93 0.6669957449954858 1106 94-95 0.8262513945478815 1106 96-97 0.9797284181776718 1106 98-99 0.8835250768688248 1106 100-101 0.8281562614482105 1107 1 0.23244404892933446 1107 2 0.18445262844658927 1107 3 0.1886693913341091 1107 4 0.11698465412170833 1107 5 0.03509612428607056 1107 6 0.14626837288125216 1107 7 0.08100330784258603 1107 8 0.2229045763074069 1107 9 0.2508376950690234 1107 10-11 0.2559715674720522 1107 12-13 0.22152386145639724 1107 14-15 0.2725652451437739 1107 16-17 0.23582710864093315 1107 18-19 0.36483192767318684 1107 20-21 0.24303227000984862 1107 22-23 0.26329391608377506 1107 24-25 0.28290019542371425 1107 26-27 0.3785483332655204 1107 28-29 0.4219865460313841 1107 30-31 0.3846841595353183 1107 32-33 0.28591277312450813 1107 34-35 0.38320002694040056 1107 36-37 0.38559440197387573 1107 38-39 0.2607252974539165 1107 40-41 0.2179001955637787 1107 42-43 0.11427343413392066 1107 44-45 0.17365014485209684 1107 46-47 0.41013528004251043 1107 48-49 0.5139074167536108 1107 50-51 0.4216563397626203 1107 52-53 0.5837269882090581 1107 54-55 0.6313995200485039 1107 56-57 0.60612373479551 1107 58-59 0.6977870012093277 1107 60-61 0.8290856778172255 1107 62-63 1.0060063218440867 1107 64-65 0.7578301649183139 1107 66-67 0.89142700691351 1107 68-69 1.149178366172766 1107 70-71 1.0450313850586461 1107 72-73 1.1744900791077306 1107 74-75 1.2145951530250585 1107 76-77 1.2889663017150461 1107 78-79 1.5392531198094836 1107 80-81 1.3965700971382375 1107 82-83 1.3171245141285546 1107 84-85 1.7244545149514892 1107 86-87 1.6238805933072733 1107 88-89 1.753699356139876 1107 90-91 1.9249303042454216 1107 92-93 2.15301379722192 1107 94-95 2.2050571391681473 1107 96-97 2.0412609234941357 1107 98-99 1.9952559665983252 1107 100-101 1.974078486506862 1108 1 0.12275537303179362 1108 2 0.1176120088720971 1108 3 0.12757647198775857 1108 4 0.12612129502823421 1108 5 0.08637558681196822 1108 6 0.13168130724469052 1108 7 0.07633963487106143 1108 8 0.0528353204646379 1108 9 0.15080542478539627 1108 10-11 0.13600071168880135 1108 12-13 0.17405083406629984 1108 14-15 0.26394313390441226 1108 16-17 0.2610687898815769 1108 18-19 0.16860822393457653 1108 20-21 0.09532841473927078 1108 22-23 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2316 90-91 -1.5104633955184532 2316 92-93 -1.8940230225404768 2316 94-95 -1.8666472598144068 2316 96-97 -1.8047731800840765 2316 98-99 -1.3470442785703582 2316 100-101 -1.8011773355429472 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 31.0 5 85.0 6 188.0 7 407.0 8 931.0 9 1682.0 10 2693.0 11 4132.0 12 5865.0 13 8170.0 14 10661.0 15 14190.0 16 18419.0 17 23471.0 18 20824.0 19 16211.0 20 14975.0 21 15971.0 22 17322.0 23 19672.0 24 22483.0 25 26202.0 26 31179.0 27 37027.0 28 43769.0 29 51235.0 30 61002.0 31 71717.0 32 85738.0 33 104679.0 34 131302.0 35 168442.0 36 208517.0 37 214238.0 38 85829.0 39 2390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.134645035452024 19.30580069159173 15.365440262158803 22.194114010797446 2 16.178181749745768 21.24547591992062 38.56849247949765 24.00784985083596 3 19.209815361962555 25.3746436482634 30.04731897007456 25.36822201969948 4 13.356736617057932 17.526257342715404 35.5942669738672 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25.744068180329133 30.22015375344051 21.617498908572426 26-27 21.822651087576276 26.19689174962491 30.125373060748984 21.855084102049833 28-29 21.74348625900326 26.24203458081657 30.353209259605208 21.661269900574965 30-31 21.72058292675728 25.99234773222836 30.1871812028078 22.09988813820656 32-33 21.629530794271233 26.05654884009656 30.047024032884067 22.26689633274814 34-35 22.426385562841773 26.104017886538035 29.660793470232942 21.80880308038725 36-37 22.386608118383798 25.68990820688098 29.731095730075808 22.192387944659416 38-39 21.846185681096753 26.05971501788233 29.836135120171797 22.25796418084912 40-41 22.180037913984876 25.8557160440373 29.88144176927605 22.082804272701768 42-43 22.197504741691336 25.930435301535244 29.460822342901473 22.411237613871947 44-45 22.008858125651752 26.222484589099416 29.345046861138908 22.42361042410993 46-47 22.22921322077105 26.54766113260663 28.70247427294516 22.52065137367716 48-49 22.45328913869096 26.554439518312993 28.455760492714045 22.536510850282003 50-51 22.46102073553517 26.804086263251197 28.108659990283158 22.626233010930473 52-53 22.874976081055223 26.807412764770554 27.830786844093886 22.486824310080337 54-55 22.2025317851547 26.88219262674575 28.062202609624364 22.85307297847519 56-57 22.32936468039425 27.16196449942108 27.99148972052943 22.517181099655243 58-59 22.438420057749596 26.50431337768307 28.05439141197793 23.002875152589404 60-61 22.184780740303502 26.828104679032084 28.348181998034594 22.63893258262982 62-63 22.157678851881236 26.951053932078416 28.183544348961053 22.70772286707929 64-65 21.88733838059464 26.73011424456511 28.086938751678638 23.29560862316161 66-67 21.906030873281246 27.037328108321475 27.867072273240183 23.189568745157096 68-69 22.03935172195005 26.69435350116923 28.09910948215153 23.167185294729197 70-71 21.939792996465833 27.05831910898902 27.745271425786868 23.25661646875828 72-73 22.181836579878436 26.90767024861765 27.71802814425577 23.192465027248147 74-75 21.915267707978526 27.24571391497575 27.46069128833958 23.378327088706147 76-77 21.94772144016702 26.975729086256024 27.84780635705263 23.228743116524335 78-79 22.1034764446688 27.05124120734414 27.698009539313823 23.147272808673236 80-81 21.91254744093869 27.385769486429435 27.578761122809702 23.122921949822175 82-83 21.987374068368727 27.208402493261367 27.429314119526527 23.374909318843383 84-85 21.851528316287887 26.955495863063796 27.49629643085249 23.696679389795825 86-87 22.128154120462888 26.893850809165514 27.375363729573003 23.602631340798595 88-89 21.87582568750759 27.013736194180275 27.271399385737183 23.83903873257495 90-91 21.676279950949542 26.949496194696582 27.629941541715596 23.74428231263828 92-93 21.801077475112542 26.61646408183802 27.570827914436524 24.01163052861291 94-95 21.54328631394066 26.551482617769352 27.578380703633137 24.32685036465685 96-97 21.8875318284108 26.43286249347858 27.430769150904954 24.24883652720567 98-99 21.56279102971383 26.548075269410365 27.160945313184477 24.72818838769133 100-101 21.5224010636852 26.28699194613326 27.71611619962846 24.47449079055308 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1420.0 1 951.5 2 604.5 3 770.0 4 708.0 5 672.5 6 1136.5 7 2426.5 8 3655.0 9 4042.5 10 3416.5 11 2560.5 12 2507.0 13 2731.0 14 2933.5 15 3095.5 16 3215.5 17 3345.0 18 3509.5 19 3768.5 20 4033.5 21 4426.0 22 5059.0 23 5712.5 24 6494.5 25 7606.5 26 8997.5 27 10698.5 28 12179.5 29 13699.5 30 15787.0 31 18216.5 32 21286.0 33 23272.5 34 24899.0 35 26792.5 36 28791.0 37 31479.0 38 33890.0 39 35317.0 40 36702.0 41 40773.5 42 45682.5 43 49721.5 44 52712.5 45 55187.5 46 55807.5 47 57288.5 48 61830.0 49 63021.0 50 61812.0 51 59986.0 52 57180.0 53 56495.0 54 56628.5 55 52851.5 56 46461.5 57 40952.0 58 37368.0 59 33744.0 60 28370.5 61 22930.5 62 18780.5 63 14668.0 64 11064.5 65 8843.5 66 6669.0 67 5234.0 68 4013.5 69 2825.5 70 2202.5 71 1799.5 72 1579.0 73 1374.0 74 1146.5 75 993.5 76 690.5 77 363.0 78 208.5 79 135.5 80 77.0 81 36.5 82 18.0 83 17.0 84 12.0 85 5.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.594597399564756E-4 2 0.048259511631904464 3 0.0 4 6.48649349891189E-5 5 0.045535184362361474 6 6.48649349891189E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006875683108846603 26-27 0.0016864883097170917 28-29 6.48649349891189E-5 30-31 0.001978380517168127 32-33 4.216220774292729E-4 34-35 0.011059471415644775 36-37 0.024194620750941354 38-39 0.0038594636318525746 40-41 0.001135136362309581 42-43 0.001362163634771497 44-45 0.009243253235949444 46-47 0.0 48-49 0.0 50-51 7.78379219869427E-4 52-53 0.0 54-55 0.0016540558422225321 56-57 0.0 58-59 0.02305948438863177 60-61 0.0 62-63 0.009859470118346075 64-65 0.015243259722442943 66-67 0.006843250641352045 68-69 0.016767585694687236 70-71 3.243246749455945E-5 72-73 3.243246749455945E-5 74-75 0.022054077896300428 76-77 0.048421673969377264 78-79 0.03009732983495117 80-81 0.026367596073076836 82-83 0.03574057917900452 84-85 0.015989206474817812 86-87 0.010994606480655655 88-89 0.08315684665605044 90-91 0.02562164932070197 92-93 0.024421648023403266 94-95 0.027340570097913616 96-97 0.03820544670859104 98-99 0.05189194799129512 100-101 0.015016232449981026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1541665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.02888733593144 #Duplication Level Percentage of deduplicated Percentage of total 1 73.49262231991017 33.827836327853056 2 13.7698028542991 12.676174084370405 3 4.944329014601679 6.827458894946327 4 2.370625093858885 4.364689414438501 5 1.3736051271940017 3.1612757821835236 6 0.8425343885940875 2.3268552269547076 7 0.5625026387731726 1.8123959410378154 8 0.4062818660144356 1.4960561789928355 9 0.28044326803148595 1.161764242950752 >10 1.6879813994724053 14.407708317968329 >50 0.163568996546031 5.226117610336834 >100 0.09822344394845477 8.463742675120903 >500 0.005644972646180726 1.724893470322985 >1k 0.0014112431615451815 0.9268200660754822 >5k 2.8224863230903627E-4 0.932991071561687 >10k+ 1.4112431615451813E-4 0.6632206948858055 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10210 0.662270986238904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8306 0.5387681500196216 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6057 0.39288691122909325 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2000 0.12972986997823782 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1941 0.12590283881387979 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1777 0.11526498947566431 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.243246749455945E-5 0.0 12-13 0.0 0.0 0.0 6.48649349891189E-5 0.0 14-15 0.0 0.0 0.0 6.48649349891189E-5 0.0 16-17 0.0 0.0 0.0 1.297298699782378E-4 0.0 18-19 0.0 0.0 0.0 1.297298699782378E-4 0.0 20-21 0.0 0.0 0.0 3.243246749455945E-4 0.0 22-23 0.0 0.0 0.0 8.432441548585458E-4 0.0 24-25 0.0 0.0 0.0 0.001718920777211651 6.48649349891189E-5 26-27 0.0 0.0 0.0 0.0035027064894124207 6.48649349891189E-5 28-29 0.0 0.0 0.0 0.00713514284880308 6.48649349891189E-5 30-31 0.0 0.0 0.0 0.01907029088680096 9.729740248367836E-5 32-33 0.0 0.0 0.0 0.040605449303188434 1.297298699782378E-4 34-35 0.0 0.0 0.0 0.07449737783500307 1.297298699782378E-4 36-37 0.0 0.0 0.0 0.12943797777078678 1.297298699782378E-4 38-39 0.0 0.0 0.0 0.21428131273655432 1.297298699782378E-4 40-41 0.0 0.0 0.0 0.3081084411983148 1.297298699782378E-4 42-43 0.0 0.0 0.0 0.4056653034219496 1.297298699782378E-4 44-45 0.0 0.0 0.0 0.5230059708172657 1.297298699782378E-4 46-47 0.0 0.0 0.0 0.6643142316910613 1.297298699782378E-4 48-49 0.0 0.0 0.0 0.8208657522872997 1.297298699782378E-4 50-51 0.0 0.0 0.0 0.990033502738922 1.297298699782378E-4 52-53 0.0 0.0 0.0 1.156249898648539 1.297298699782378E-4 54-55 0.0 0.0 0.0 1.3333636036363283 1.297298699782378E-4 56-57 0.0 0.0 0.0 1.5414503150814216 1.297298699782378E-4 58-59 0.0 0.0 0.0 1.7700343459830767 1.297298699782378E-4 60-61 0.0 0.0 0.0 2.0194075885487446 1.297298699782378E-4 62-63 0.0 0.0 0.0 2.2645646103401194 1.6216233747279725E-4 64-65 0.0 0.0 0.0 2.522045969779427 1.9459480496735672E-4 66-67 0.0 0.0 0.0 2.8186084525496784 1.9459480496735672E-4 68-69 0.0 0.0 0.0 3.1431601547677346 1.9459480496735672E-4 70-71 0.0 0.0 0.0 3.490479449166972 1.9459480496735672E-4 72-73 0.0 0.0 0.0 3.8472041591396318 1.9459480496735672E-4 74-75 0.0 0.0 0.0 4.2032802197624 1.9459480496735672E-4 76-77 0.0 0.0 0.0 4.598761728391057 1.9459480496735672E-4 78-79 0.0 0.0 0.0 5.0366000395676105 1.9459480496735672E-4 80-81 0.0 0.0 0.0 5.499151890975018 1.9459480496735672E-4 82-83 0.0 0.0 0.0 5.984633496901077 1.9459480496735672E-4 84-85 0.0 0.0 0.0 6.46301239244583 2.2702727246191618E-4 86-87 0.0 0.0 0.0 6.949012917851803 2.594597399564756E-4 88-89 0.0 0.0 0.0 7.483045927617219 2.594597399564756E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4715 0.0 52.499493 1 GTATCAA 8635 0.0 43.137287 1 ATCAACG 11585 0.0 32.139263 3 TATCAAC 11585 0.0 32.111813 2 ATCCGCG 60 0.0039623734 31.648676 7 TCAACGC 11830 0.0 31.513802 4 CAACGCA 12065 0.0 30.89998 5 AACGCAG 12435 0.0 30.00707 6 ACGCAGA 14315 0.0 25.96673 7 CGCAGAG 14395 0.0 25.657522 8 TTGTACG 130 1.006825E-5 25.562393 9 GTACATG 9330 0.0 24.952469 1 GTATAGA 310 0.0 24.522083 1 GCAGAGT 14975 0.0 24.029745 9 TACATGG 9645 0.0 23.891235 2 AGTACTT 8360 0.0 22.79954 12-13 GAGTACT 8205 0.0 22.738447 12-13 ACATGGG 9845 0.0 21.99708 3 AGAGTAC 13645 0.0 21.67511 10-11 TGGTATC 2165 0.0 21.50638 2 >>END_MODULE