FastQCFastQC Report
Thu 26 May 2016
SRR938650_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938650_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570590
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108710.6921602709809689No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81860.5212054068853106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58150.37024302968947975No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18730.1192545476540663No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16840.10722085330990266No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG16820.10709351262901204No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.10053546756314505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44000.054.6247181
GTATCAA84900.045.295411
TATCAAC112850.034.1122782
ATCAACG113950.033.6995623
TCAACGC117250.032.7510874
CAACGCA119550.032.1199725
AACGCAG122000.031.5478256
TGGTATC19250.028.3918912
GTACATG95650.028.0139241
ACGCAGA139200.027.6120197
CGCAGAG140500.027.3903478
TACATGG96850.027.0873622
GTGGTAT20750.027.0653971
GCAGAGT145850.025.7642429
ACATGGG100200.024.8536873
TACCCCG1401.7730612E-523.7620225
CATGGGG44050.023.6279164
GAGTACT81900.023.43167512-13
AGTACTT84300.023.10266512-13
AGAGTAC134450.022.38376610-11
Produced by FastQC (version 0.11.5)