##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938650_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1570590 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.161308170814788 31.0 31.0 34.0 28.0 34.0 2 31.221861211391897 31.0 31.0 34.0 28.0 34.0 3 31.27160175475458 31.0 31.0 34.0 27.0 34.0 4 34.939814973990664 37.0 35.0 37.0 32.0 37.0 5 34.9646126614839 37.0 35.0 37.0 32.0 37.0 6 35.02071005163665 37.0 35.0 37.0 32.0 37.0 7 34.946295977944594 37.0 35.0 37.0 32.0 37.0 8 34.912390884954064 37.0 35.0 37.0 32.0 37.0 9 36.41426788659039 39.0 37.0 39.0 32.0 39.0 10-11 36.26506631265957 39.0 35.0 39.0 32.0 39.0 12-13 36.112111372159504 39.0 35.0 39.0 31.0 39.0 14-15 37.07038565125208 39.0 36.0 41.0 31.0 41.0 16-17 36.94869730483448 39.0 36.0 41.0 31.0 41.0 18-19 36.98730604422542 39.0 36.0 41.0 31.0 41.0 20-21 37.096028562514725 39.0 36.0 41.0 31.0 41.0 22-23 37.01350065898802 39.0 36.0 41.0 31.0 41.0 24-25 36.892154540650324 39.0 36.0 41.0 30.5 41.0 26-27 36.702353892486265 39.0 36.0 41.0 30.0 41.0 28-29 36.50934744268078 39.0 35.5 41.0 30.0 41.0 30-31 36.25614450620468 39.0 35.0 40.0 30.0 41.0 32-33 35.944085662076034 38.0 35.0 40.0 29.0 41.0 34-35 35.66576477629425 38.0 35.0 40.0 27.5 41.0 36-37 35.35906251790728 38.0 34.0 40.0 26.5 41.0 38-39 35.0880462119331 38.0 34.0 40.0 25.5 41.0 40-41 34.97159761618246 38.0 34.0 40.0 25.0 41.0 42-43 34.73906875760065 38.0 33.5 40.0 24.5 41.0 44-45 34.35707823174731 38.0 33.0 40.0 23.5 41.0 46-47 33.877393845624894 38.0 33.0 40.0 22.0 41.0 48-49 33.440636003030704 38.0 33.0 40.0 18.5 41.0 50-51 32.648923971246475 36.5 31.5 39.5 16.0 40.5 52-53 32.70338153178105 37.0 31.5 39.5 15.5 40.0 54-55 32.82368281983204 37.0 32.0 40.0 14.0 41.0 56-57 32.528084350467026 37.0 31.5 40.0 11.0 41.0 58-59 32.20706040405198 36.0 31.0 39.5 8.0 41.0 60-61 31.769223985890655 36.0 31.0 39.0 7.0 41.0 62-63 31.259827835399435 35.0 30.0 39.0 4.0 40.0 64-65 30.775760064689067 35.0 29.0 38.5 2.0 40.0 66-67 30.2505864038355 35.0 29.0 38.0 2.0 40.0 68-69 29.713371089845218 34.0 28.0 37.5 2.0 40.0 70-71 29.10372439656435 34.0 27.5 37.0 2.0 39.0 72-73 28.559113135827936 34.0 26.0 36.0 2.0 39.0 74-75 27.975002387637765 33.5 26.0 36.0 2.0 38.5 76-77 27.270674396245997 33.0 25.0 35.0 2.0 37.5 78-79 26.5153130352288 32.0 23.5 35.0 2.0 37.0 80-81 25.87353765145582 32.0 21.0 35.0 2.0 36.0 82-83 25.23365900712471 31.0 19.0 35.0 2.0 36.0 84-85 24.595410960212405 31.0 17.0 34.0 2.0 35.0 86-87 23.923414767698763 31.0 9.0 34.0 2.0 35.0 88-89 23.148738053852373 30.0 4.0 34.0 2.0 35.0 90-91 22.3270519358967 29.5 2.0 34.0 2.0 35.0 92-93 21.48913783992003 29.0 2.0 34.0 2.0 35.0 94-95 20.540532538727483 28.0 2.0 34.0 2.0 35.0 96-97 19.3103088648215 26.0 2.0 33.5 2.0 35.0 98-99 17.852627674950178 24.0 2.0 33.0 2.0 34.5 100-101 14.485174679579012 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5739689428270225 1101 2 0.6132297521142611 1101 3 0.5990187073577928 1101 4 0.5461531694373178 1101 5 0.42285500199001547 1101 6 0.5884921940894614 1101 7 0.3425484177964151 1101 8 0.46474958830465596 1101 9 0.5052862882399936 1101 10-11 0.8624458577536842 1101 12-13 0.7335074055476767 1101 14-15 0.893080037208442 1101 16-17 0.934676813322298 1101 18-19 0.8631135312099403 1101 20-21 0.9195769254437138 1101 22-23 1.0736532378607606 1101 24-25 1.0228103360259766 1101 26-27 0.9697028348745462 1101 28-29 1.056610368377143 1101 30-31 1.0056938244251867 1101 32-33 0.9785688150547429 1101 34-35 1.0395967494997294 1101 36-37 1.144736601286013 1101 38-39 1.1459982882461404 1101 40-41 1.059169271633607 1101 42-43 1.1265602762768339 1101 44-45 1.105175256684575 1101 46-47 1.222188390998447 1101 48-49 0.9852066128622354 1101 50-51 1.0828807517615147 1101 52-53 1.0214790463806693 1101 54-55 1.0297766895868605 1101 56-57 0.9092249418273184 1101 58-59 0.8648239883183493 1101 60-61 0.8181149715785203 1101 62-63 0.9830719448391392 1101 64-65 0.9651885669134685 1101 66-67 0.7512066471008616 1101 68-69 0.9225017868885388 1101 70-71 1.0378165638151806 1101 72-73 1.1928715818949414 1101 74-75 1.0388971238642206 1101 76-77 0.984219429950425 1101 78-79 0.7383997027003488 1101 80-81 0.8683777961233901 1101 82-83 0.6796442781720025 1101 84-85 0.9120772606137599 1101 86-87 0.978161964450468 1101 88-89 1.1193299426217038 1101 90-91 1.1536785602835593 1101 92-93 1.4441090097574616 1101 94-95 1.026662942100085 1101 96-97 1.1800608288227998 1101 98-99 1.2074608402982712 1101 100-101 0.9618860973400789 1102 1 0.6553614017979648 1102 2 0.6768264049307717 1102 3 0.647976740177711 1102 4 0.5745208034501701 1102 5 0.5206595003974783 1102 6 0.6190320587684042 1102 7 0.6822739707306908 1102 8 0.6326782552136052 1102 9 0.7629828661649114 1102 10-11 0.7196495832666585 1102 12-13 0.7875382089274936 1102 14-15 0.7746337274819908 1102 16-17 0.9617537226934871 1102 18-19 1.0766713228281546 1102 20-21 0.7953041418953788 1102 22-23 0.8690068789281611 1102 24-25 0.9101045350710777 1102 26-27 0.8434742943945892 1102 28-29 0.879009488494674 1102 30-31 0.5264534474111997 1102 32-33 0.6110603153384417 1102 34-35 0.7812597007277802 1102 36-37 0.8489379401760573 1102 38-39 0.7814941941866351 1102 40-41 0.7625875584188435 1102 42-43 0.8918869288976907 1102 44-45 1.0874820674061922 1102 46-47 1.096885275733257 1102 48-49 0.7715014525863282 1102 50-51 0.83869546523146 1102 52-53 0.9210112965959993 1102 54-55 0.7393782155601727 1102 56-57 0.7064542029150402 1102 58-59 1.1595979377026282 1102 60-61 0.9766986338894874 1102 62-63 0.8284634896248448 1102 64-65 0.8323708989933323 1102 66-67 0.8674109966410022 1102 68-69 0.8374274011015359 1102 70-71 0.6661115958678643 1102 72-73 0.7547843188348118 1102 74-75 0.6494783600041778 1102 76-77 0.7337126940787684 1102 78-79 0.6502112173545811 1102 80-81 0.6737554199827898 1102 82-83 0.7081711452683663 1102 84-85 0.6300517408582458 1102 86-87 0.3234929382200953 1102 88-89 0.6101377185247614 1102 90-91 0.5034824225912757 1102 92-93 0.5999971577788727 1102 94-95 0.5356493080014779 1102 96-97 0.7475192481732229 1102 98-99 0.8171855481523913 1102 100-101 1.0331142818872685 1103 1 0.5436432919919021 1103 2 0.5844654835067544 1103 3 0.5320824793503718 1103 4 0.4366859677950643 1103 5 0.5918446653493774 1103 6 0.5366016359938115 1103 7 0.4595625075603422 1103 8 0.26931305444878717 1103 9 0.5375726867967998 1103 10-11 0.4859866496419656 1103 12-13 0.4256389100109885 1103 14-15 0.7043572395764457 1103 16-17 0.7614150174217116 1103 18-19 0.6922893992492973 1103 20-21 0.5766814219342322 1103 22-23 0.6247147911561513 1103 24-25 0.6540257466404782 1103 26-27 0.5686950901698751 1103 28-29 0.4952928354854791 1103 30-31 0.7272838216258961 1103 32-33 0.8486220015808286 1103 34-35 0.729712764553696 1103 36-37 0.7055593854399547 1103 38-39 0.5527333820223816 1103 40-41 0.7162316750839963 1103 42-43 0.7391933721888364 1103 44-45 0.7675233433174427 1103 46-47 0.6622481092031691 1103 48-49 0.677932603759352 1103 50-51 0.9938485806522621 1103 52-53 0.963970415233895 1103 54-55 0.9663690195299424 1103 56-57 1.1403781459785662 1103 58-59 1.0327273194744606 1103 60-61 1.0226593610739236 1103 62-63 0.8938062921378176 1103 64-65 1.041091649243718 1103 66-67 1.0693400384935607 1103 68-69 1.0669930935352987 1103 70-71 1.08595316920848 1103 72-73 1.3369519111744879 1103 74-75 1.2390155234992797 1103 76-77 1.2665756034235187 1103 78-79 1.0894075510824628 1103 80-81 1.252505609669882 1103 82-83 1.0997174744437608 1103 84-85 0.8893385173916748 1103 86-87 1.1915666278616648 1103 88-89 1.0311495853492474 1103 90-91 1.4477981833123934 1103 92-93 1.4360704832212257 1103 94-95 1.167655135188948 1103 96-97 1.361164578835762 1103 98-99 1.6729905801715752 1103 100-101 1.2497442251328899 1104 1 0.24875226578524945 1104 2 0.3349052023220125 1104 3 0.26495501481710093 1104 4 0.3554047536417073 1104 5 0.22910419259036985 1104 6 0.38623446833201314 1104 7 0.24717743741121723 1104 8 0.12773594923980625 1104 9 0.32350874373965155 1104 10-11 0.38910027063082 1104 12-13 0.30281823881477266 1104 14-15 0.43849275336606297 1104 16-17 0.4045504037566019 1104 18-19 0.4801017858881167 1104 20-21 0.2518061805055609 1104 22-23 0.43087614884314007 1104 24-25 0.32041907193435293 1104 26-27 0.35440055209522114 1104 28-29 0.43699935626549546 1104 30-31 0.2944575620300256 1104 32-33 0.20195827617713036 1104 34-35 0.17138516668317294 1104 36-37 0.30723923876897175 1104 38-39 0.30301839040497924 1104 40-41 0.3777279936031732 1104 42-43 0.3355128717819582 1104 44-45 0.3923256414242502 1104 46-47 0.5101365765110302 1104 48-49 0.532511660006108 1104 50-51 0.18729623107335414 1104 52-53 0.35492426681776834 1104 54-55 0.549706176505147 1104 56-57 0.5736057899288767 1104 58-59 0.5278230575843992 1104 60-61 0.5579635366869304 1104 62-63 0.5153839855993922 1104 64-65 0.7076806665677609 1104 66-67 0.7410894036709692 1104 68-69 0.7351315681963193 1104 70-71 0.7732275008064704 1104 72-73 0.7437381892022756 1104 74-75 0.7268977161127843 1104 76-77 0.8479760367695945 1104 78-79 0.7595082403184215 1104 80-81 0.8933948515103296 1104 82-83 0.7502005278668982 1104 84-85 0.7415231894898717 1104 86-87 0.8167812910380299 1104 88-89 1.0382501863473195 1104 90-91 0.7348872153427486 1104 92-93 0.6761526425004156 1104 94-95 0.9214284814638383 1104 96-97 0.8492867785083007 1104 98-99 0.8463852878590572 1104 100-101 0.7192999963810642 1105 1 0.46681981335573397 1105 2 0.5037864445195588 1105 3 0.5082106143908938 1105 4 0.538697189202054 1105 5 0.4509747665338537 1105 6 0.5701699475077433 1105 7 0.465932813991067 1105 8 0.4560602850630886 1105 9 0.4592760388324777 1105 10-11 0.4759794564323556 1105 12-13 0.5436046703332451 1105 14-15 0.5200566593241973 1105 16-17 0.5654921286287262 1105 18-19 0.7037074206371088 1105 20-21 0.6113718741473448 1105 22-23 0.6702794581105422 1105 24-25 0.6187780604562292 1105 26-27 0.7308012619437179 1105 28-29 0.8296217090288494 1105 30-31 0.722070183300481 1105 32-33 0.7129465591984356 1105 34-35 0.7525555209916206 1105 36-37 0.778582931212739 1105 38-39 0.7637044653197549 1105 40-41 0.6739768436448799 1105 42-43 0.6132019081309039 1105 44-45 0.6170481119358655 1105 46-47 0.6359986486938851 1105 48-49 0.4979968630985354 1105 50-51 0.37258161666626677 1105 52-53 0.39501055334351776 1105 54-55 0.34541088400987263 1105 56-57 0.5813784816616803 1105 58-59 0.7416094947010379 1105 60-61 0.8400803556550365 1105 62-63 0.8037588169543923 1105 64-65 0.9287682425396468 1105 66-67 0.8850634356066855 1105 68-69 0.4412212621441327 1105 70-71 0.5147058666620836 1105 72-73 0.4046937229042129 1105 74-75 0.45338659720570007 1105 76-77 0.5567017243653467 1105 78-79 0.7011823556669334 1105 80-81 0.44945664788980366 1105 82-83 0.5405931278269662 1105 84-85 0.638349211296628 1105 86-87 0.8778314338847757 1105 88-89 0.6042019039604796 1105 90-91 0.5970946874125218 1105 92-93 0.6741292757211035 1105 94-95 0.9193541712988598 1105 96-97 1.173325898549951 1105 98-99 1.4248181349598532 1105 100-101 1.3068832458972892 1106 1 0.2378866259138519 1106 2 0.3692497556828691 1106 3 0.18494217287755532 1106 4 0.2616444703555416 1106 5 0.380642514938323 1106 6 -0.014627590760760256 1106 7 0.17931175707472846 1106 8 0.16793686985875667 1106 9 0.1892261095997938 1106 10-11 0.13951599215771182 1106 12-13 0.29752189311770394 1106 14-15 0.397409999205216 1106 16-17 0.18660543475056812 1106 18-19 -0.29062111147332814 1106 20-21 0.1568401520929612 1106 22-23 0.09462061257000443 1106 24-25 0.1663488184320201 1106 26-27 0.05993648165848953 1106 28-29 0.09178939208091919 1106 30-31 0.23144769236578355 1106 32-33 0.3985884504595063 1106 34-35 0.18982636747323767 1106 36-37 0.07558194905955418 1106 38-39 0.16235372459698993 1106 40-41 0.3201673589578746 1106 42-43 -0.06645930503368191 1106 44-45 0.2630265120344575 1106 46-47 -0.023904106936683434 1106 48-49 0.3251504113603474 1106 50-51 0.3176243571350881 1106 52-53 0.39205270377353685 1106 54-55 0.06416347476568518 1106 56-57 -0.14077691513941915 1106 58-59 -0.3317226187208462 1106 60-61 0.5146074843693818 1106 62-63 0.5201951319753633 1106 64-65 0.6186836205192527 1106 66-67 -0.1664915780426277 1106 68-69 -0.20493639024832788 1106 70-71 -0.050691718660139884 1106 72-73 0.33912547446053765 1106 74-75 0.045031573100001054 1106 76-77 0.2083928577253218 1106 78-79 0.6200275601079106 1106 80-81 0.40779393348725534 1106 82-83 0.4170800816209521 1106 84-85 0.7874947753625179 1106 86-87 0.7625727690903013 1106 88-89 0.3449534192025254 1106 90-91 0.5714746798689916 1106 92-93 0.5616225070798002 1106 94-95 0.4263631227102884 1106 96-97 0.44767153431675766 1106 98-99 0.1345272607688237 1106 100-101 0.1492984464918088 1107 1 0.35736711286709166 1107 2 0.17423788829703568 1107 3 0.20010752806672016 1107 4 0.14953119170107243 1107 5 0.19385310321001725 1107 6 0.24683869068855557 1107 7 0.18674452697120358 1107 8 0.0111326809883181 1107 9 0.13333521588471342 1107 10-11 0.2802292286759638 1107 12-13 0.17415301410316175 1107 14-15 0.17022212029232975 1107 16-17 0.2391526869829761 1107 18-19 0.30891539013035185 1107 20-21 0.2685803189582785 1107 22-23 0.33142799848913995 1107 24-25 0.24613691450849018 1107 26-27 0.11967414687003242 1107 28-29 0.03141747366017711 1107 30-31 0.2572395512359762 1107 32-33 0.32430053044171103 1107 34-35 0.3239871274573787 1107 36-37 0.29530133068846 1107 38-39 0.545646714220581 1107 40-41 0.24742602966333038 1107 42-43 0.3769717175357101 1107 44-45 0.2753134184257604 1107 46-47 0.30816501708670074 1107 48-49 0.23818479363013267 1107 50-51 0.12955870419313698 1107 52-53 0.24297262875338532 1107 54-55 0.12472250156638154 1107 56-57 0.2585889789808178 1107 58-59 0.15085604414668552 1107 60-61 0.3948857454695549 1107 62-63 0.3879813304487314 1107 64-65 0.336009635508546 1107 66-67 0.29114642175405336 1107 68-69 0.41735293234567195 1107 70-71 0.2708297229288945 1107 72-73 0.2518663028350048 1107 74-75 0.32809303451957916 1107 76-77 0.25851801407867825 1107 78-79 0.38054798659715416 1107 80-81 0.21040011749287757 1107 82-83 0.49325418670514054 1107 84-85 0.5150395598269242 1107 86-87 0.3876119955518469 1107 88-89 0.6184027400875038 1107 90-91 0.7725436285703537 1107 92-93 0.8095112763044447 1107 94-95 0.8519584441168142 1107 96-97 0.9005349555124518 1107 98-99 0.8251641806407655 1107 100-101 0.06464442756587019 1108 1 0.13313229310463015 1108 2 0.1674550124519456 1108 3 0.25479258995742526 1108 4 0.32616983324058424 1108 5 0.2587924622432496 1108 6 0.22011138900625582 1108 7 0.3192766220857166 1108 8 0.13657089347415052 1108 9 0.0757346885657526 1108 10-11 0.17901526672090995 1108 12-13 0.14402516924806008 1108 14-15 0.21596118452969648 1108 16-17 0.12519030344984117 1108 18-19 0.21316493540344084 1108 20-21 0.2351804756399929 1108 22-23 0.1891582845511124 1108 24-25 0.22502273181361687 1108 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-1.08134722253439 2316 60-61 -1.2366690188356344 2316 62-63 -0.9626705601318335 2316 64-65 -1.1242207473510533 2316 66-67 -1.0670451654090165 2316 68-69 -0.8966299248445111 2316 70-71 -1.3547868362061948 2316 72-73 -0.7192317465379112 2316 74-75 -0.736361347822001 2316 76-77 -0.8857201179814922 2316 78-79 -0.6722483363725402 2316 80-81 -0.5682490354183045 2316 82-83 -0.6528947045613442 2316 84-85 -0.5953353487856674 2316 86-87 -0.665554771129603 2316 88-89 -0.8850916283398469 2316 90-91 -0.9915520558356974 2316 92-93 -1.097158366962777 2316 94-95 -0.7327515669739135 2316 96-97 -0.9995980879913411 2316 98-99 -1.297206854877409 2316 100-101 -0.9963982208958484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4586.0 3 1477.0 4 1518.0 5 1802.0 6 2108.0 7 2819.0 8 3501.0 9 4580.0 10 5926.0 11 7646.0 12 9909.0 13 12262.0 14 14257.0 15 17560.0 16 20980.0 17 25030.0 18 22283.0 19 18444.0 20 18761.0 21 20120.0 22 22279.0 23 24767.0 24 27711.0 25 30954.0 26 35298.0 27 40924.0 28 46729.0 29 53636.0 30 62616.0 31 72714.0 32 86203.0 33 103751.0 34 128137.0 35 159229.0 36 186915.0 37 183545.0 38 85289.0 39 4324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.709317011065494 18.374734333659067 14.57235692288851 22.343591732386926 2 15.75772053202874 20.313344035060897 39.73245681088519 24.196478622025175 3 19.219687882797118 25.105057205774532 29.519338997960965 26.155915913467386 4 12.922267005824416 16.66969258573384 36.06188418565511 34.34615622278663 5 13.915887088348244 37.549596980732034 32.77860315534908 15.755912775570636 6 31.9671269463344 35.469780249536015 17.25306504463373 15.310027759495856 7 29.144718487935563 33.3848836868609 19.62246797105197 17.847929854151566 8 27.55078837830694 33.579342609371366 19.304201605737596 19.565667406584097 9 27.93148124792185 16.376521906407135 19.580216904706397 36.11177994096462 10-11 25.56564710423653 26.64383302052257 26.146032356724657 21.644487518516243 12-13 26.831576114563976 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29.612794789742686 22.281315801459588 38-39 21.895204112989386 26.0366940048626 29.720838328883115 22.347263553264902 40-41 22.222080695361097 25.865268692637837 29.7640648156227 22.14858579637837 42-43 22.245233735090146 25.882315854030164 29.387983978128023 22.484466432751667 44-45 22.018065308139196 26.26674281959397 29.241068586026064 22.474123286240772 46-47 22.261362930840978 26.58334407898612 28.654787546504796 22.500505443668104 48-49 22.4824798882075 26.46032770433444 28.462926389581828 22.59426601787623 50-51 22.511889836028832 26.81359398749028 28.057243137197467 22.61727303928342 52-53 22.855974845170188 26.772758358517457 27.93208756305582 22.43917923325654 54-55 22.161728639158643 26.872650500870947 28.16495262874865 22.800668231221756 56-57 22.277005170525456 27.117145513359315 28.091713150454652 22.514136165660577 58-59 22.41723827887204 26.35884723244601 28.23526565003324 22.98864883864871 60-61 22.17126599342465 26.649647767367906 28.57465338691591 22.604432852291534 62-63 22.1213395635158 26.851727748624427 28.370804313075904 22.656128374783872 64-65 21.921616932917622 26.628555164203032 28.305399720276515 23.14442818260283 66-67 21.986512841835022 26.951585164508057 28.04955776103751 23.012344232619412 68-69 22.10777165780509 26.546276544939353 28.28284886497501 23.063102932280557 70-71 21.998267105802395 26.86836213476516 27.9697466350742 23.163624124358247 72-73 22.32025269700559 26.711754741253042 27.97428451339269 22.993708048348683 74-75 22.00618436751612 27.069997474923568 27.824304354180658 23.099513803379654 76-77 22.177271840106826 26.809418268965953 27.882124857557773 23.13118503336945 78-79 22.35649566064527 26.936122660791717 27.699568929838076 23.007812748724938 80-81 22.206996937892516 27.25463502722404 27.611721714347205 22.926646320536243 82-83 22.36046181412058 27.063448502453358 27.4753231769205 23.10076650650556 84-85 22.317187744247654 26.738517992386555 27.545452548421327 23.398841714944464 86-87 22.575712375908687 26.7246069877963 27.44681277824137 23.25286785805364 88-89 22.43565267651428 26.733520477296718 27.455961989500622 23.37486485668838 90-91 22.31922256483032 26.767296206392043 27.53466120333787 23.37882002543977 92-93 22.567081102338385 26.32626579608109 27.598581716110772 23.508071385469755 94-95 22.378589560312 26.25357749864416 27.62744525435868 23.74038768668516 96-97 22.79999222935628 26.081178386272967 27.41416225295165 23.704667131419104 98-99 22.65368767632767 26.138400106990694 27.028110531578175 24.179801685103456 100-101 23.089310132694987 25.58517437134834 27.449916647276133 23.87559884868054 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1377.0 1 910.5 2 612.5 3 783.0 4 675.5 5 673.0 6 1188.5 7 2511.5 8 3962.5 9 4332.0 10 3593.5 11 2832.5 12 2650.0 13 2804.5 14 3043.0 15 3152.0 16 3242.0 17 3418.5 18 3608.5 19 3840.5 20 4081.5 21 4489.5 22 5028.5 23 5643.5 24 6428.0 25 7537.0 26 9029.0 27 10741.0 28 12172.0 29 13652.5 30 15936.0 31 18302.5 32 21158.5 33 23474.5 34 25279.0 35 26912.0 36 29024.0 37 32065.5 38 34315.5 39 35600.5 40 37355.0 41 41361.0 42 45890.0 43 49972.0 44 53040.0 45 55285.5 46 56281.5 47 57786.0 48 61894.5 49 63542.5 50 62533.5 51 60634.5 52 58482.0 53 58036.5 54 57292.5 55 53465.0 56 47769.0 57 42784.0 58 38994.0 59 35059.0 60 29852.0 61 24397.0 62 19900.0 63 15529.5 64 11981.0 65 9681.0 66 7377.0 67 5720.5 68 4361.5 69 3120.0 70 2476.0 71 2042.0 72 1737.5 73 1519.0 74 1210.5 75 967.5 76 714.0 77 443.5 78 273.0 79 177.5 80 99.0 81 45.0 82 26.5 83 18.0 84 15.5 85 14.5 86 10.5 87 8.0 88 5.5 89 3.0 90 1.5 91 1.5 92 2.5 93 2.5 94 2.5 95 2.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18044174482200956 2 0.04775275533398277 3 0.046543018865521875 4 0.05214600882470919 5 0.05972277933770112 6 0.06602614304178685 7 0.03845688562896746 8 0.037883852564959665 9 0.043550512864592286 10-11 0.04358234803481494 12-13 0.040589842033885354 14-15 0.03616475337293628 16-17 0.03364977492534653 18-19 0.04179957850234625 20-21 0.0525598660376037 22-23 0.04870781044066243 24-25 0.06997370414939609 26-27 0.05389694318695522 28-29 0.046224667163295326 30-31 0.05520218516608408 32-33 0.051732151611814665 34-35 0.05112728337758422 36-37 0.04861230492999446 38-39 0.02817412564704983 40-41 0.02629585060391318 42-43 0.023016828070979694 44-45 0.004202242469390484 46-47 0.011938188833495693 48-49 0.01149249645037852 50-51 0.008340814598335657 52-53 0.009932573109468415 54-55 0.011174144748151968 56-57 0.009550551066796554 58-59 0.011333320599265244 60-61 0.011396990939710555 62-63 0.004043066618277208 64-65 0.007035572619206795 66-67 0.0037565500862733114 68-69 0.00891384766234345 70-71 0.024481245901221832 72-73 0.02101121234695242 74-75 0.021679750921628178 76-77 0.013784628706409691 78-79 0.0050936272356248286 80-81 0.0065580450658669675 82-83 0.005602989959187311 84-85 0.004998121724956863 86-87 0.007863287044995828 88-89 0.0316759943715419 90-91 0.03864789665030339 92-93 0.024353905220331214 94-95 0.03396812662757308 96-97 0.03683329194761204 98-99 0.02292132256031173 100-101 0.12447551557058176 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1570590.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.38496429031379 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48298571629644 36.5224156840927 2 12.893925984046433 12.477442966000718 3 4.550069058067967 6.6046478667924085 4 2.196942525109149 4.251959425011119 5 1.2005548283731176 2.9044401249698546 6 0.7579840298748527 2.2005018130873735 7 0.5196018515419825 1.7598641922427827 8 0.36299810959016116 1.405092045597709 9 0.27050424760266406 1.177950452456979 >10 1.5253853261745989 13.757717441693329 >50 0.14392824440375115 4.855330198217861 >100 0.08849620138655434 7.972147392802118 >500 0.004901669373903714 1.6181205979190534 >1k 0.0013247755064604632 0.8980941093901068 >5k 2.6495510129209265E-4 0.8974531172343793 >10k+ 1.3247755064604632E-4 0.6968225724916033 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10871 0.6921602709809689 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8186 0.5212054068853106 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5815 0.37024302968947975 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1873 0.1192545476540663 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1684 0.10722085330990266 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1682 0.10709351262901204 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1579 0.10053546756314505 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.367034044531036E-5 0.0 14-15 0.0 0.0 0.0 6.367034044531036E-5 0.0 16-17 0.0 0.0 0.0 6.367034044531036E-5 0.0 18-19 0.0 0.0 0.0 6.367034044531036E-5 0.0 20-21 0.0 0.0 0.0 1.2734068089062072E-4 0.0 22-23 0.0 0.0 0.0 4.456923831171725E-4 0.0 24-25 0.0 0.0 0.0 0.0012415716386835521 0.0 26-27 0.0 0.0 0.0 0.002674154298703035 0.0 28-29 0.0 0.0 0.0 0.007322089151210692 0.0 30-31 0.0 0.0 0.0 0.020056157240272765 0.0 32-33 0.0 0.0 0.0 0.04342317218370166 0.0 34-35 0.0 0.0 0.0 0.07793249670505989 0.0 36-37 0.0 0.0 0.0 0.13768711121298366 0.0 38-39 0.0 0.0 0.0 0.22918139043289465 0.0 40-41 0.0 0.0 0.0 0.3278704181231257 0.0 42-43 0.0 0.0 0.0 0.4279601933031536 0.0 44-45 0.0 0.0 0.0 0.5524038737035126 0.0 46-47 0.0 0.0 0.0 0.697699590599711 0.0 48-49 0.0 0.0 0.0 0.8531188916267135 0.0 50-51 0.0 0.0 0.0 1.0242010964032624 0.0 52-53 0.0 0.0 0.0 1.194837608796694 0.0 54-55 0.0 0.0 0.0 1.3762980790658288 0.0 56-57 0.0 0.0 0.0 1.5897210602385092 0.0 58-59 0.0 0.0 0.0 1.823677726204802 0.0 60-61 0.0 0.0 0.0 2.071673702239286 0.0 62-63 0.0 0.0 0.0 2.3188101286777583 0.0 64-65 0.0 0.0 0.0 2.5769933591834917 0.0 66-67 0.0 0.0 0.0 2.8696540790403606 0.0 68-69 0.0 0.0 0.0 3.1972061454612595 0.0 70-71 0.0 0.0 0.0 3.551054062486072 0.0 72-73 0.0 0.0 0.0 3.9103457936189585 0.0 74-75 0.0 0.0 0.0 4.27282104177411 0.0 76-77 0.0 0.0 0.0 4.660350568894492 0.0 78-79 0.0 0.0 0.0 5.084013014217588 0.0 80-81 0.0 0.0 0.0 5.546450696871876 0.0 82-83 0.0 0.0 0.0 6.022290986189903 0.0 84-85 0.0 0.0 0.0 6.492114428335848 0.0 86-87 0.0 0.0 0.0 6.974289916528184 0.0 88-89 0.0 0.0 0.0 7.478017814961257 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4400 0.0 54.624718 1 GTATCAA 8490 0.0 45.29541 1 TATCAAC 11285 0.0 34.112278 2 ATCAACG 11395 0.0 33.699562 3 TCAACGC 11725 0.0 32.751087 4 CAACGCA 11955 0.0 32.119972 5 AACGCAG 12200 0.0 31.547825 6 TGGTATC 1925 0.0 28.391891 2 GTACATG 9565 0.0 28.013924 1 ACGCAGA 13920 0.0 27.612019 7 CGCAGAG 14050 0.0 27.390347 8 TACATGG 9685 0.0 27.087362 2 GTGGTAT 2075 0.0 27.065397 1 GCAGAGT 14585 0.0 25.764242 9 ACATGGG 10020 0.0 24.853687 3 TACCCCG 140 1.7730612E-5 23.762022 5 CATGGGG 4405 0.0 23.627916 4 GAGTACT 8190 0.0 23.431675 12-13 AGTACTT 8430 0.0 23.102665 12-13 AGAGTAC 13445 0.0 22.383766 10-11 >>END_MODULE