FastQCFastQC Report
Thu 26 May 2016
SRR938650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938650_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570590
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104210.6635086177805792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84880.5404338496997944No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64130.4083178932757754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21170.134790110722722No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20250.12893243940175347No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG17360.11053171101305878No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15800.10059913790359037No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47950.055.250651
GTATCAA89200.042.1243361
TATCAAC117650.031.97832
ATCAACG118350.031.6534563
TCAACGC120250.031.1137834
CAACGCA123450.030.307275
AACGCAG125100.029.9169376
GCGTATC650.00580687729.2696461
TGGTATC21250.027.5306842
GTGGTAT22950.026.1131131
ACGCAGA145050.025.7039647
CGCAGAG145750.025.515348
GTACATG93150.024.9687521
TACATGG96300.024.0038432
GCAGAGT154100.023.6704649
GAGTACT86250.022.71501512-13
ACATGGG98400.022.5623843
AGTACTT87350.022.10272812-13
GGACCGA5600.022.0512666
GTACTTT90650.021.71725514-15
Produced by FastQC (version 0.11.5)