##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938650_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1570590 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.726168509923024 31.0 31.0 34.0 30.0 34.0 2 31.9522733495056 31.0 31.0 34.0 30.0 34.0 3 31.999574682125825 33.0 31.0 34.0 30.0 34.0 4 35.70096778917477 37.0 35.0 37.0 33.0 37.0 5 35.57011441560178 37.0 35.0 37.0 33.0 37.0 6 35.61080804029059 37.0 35.0 37.0 33.0 37.0 7 35.54265976480176 37.0 35.0 37.0 33.0 37.0 8 35.53902546176914 37.0 35.0 37.0 33.0 37.0 9 37.08488020425445 39.0 37.0 39.0 33.0 39.0 10-11 37.002040952762975 39.0 37.0 39.0 33.0 39.0 12-13 36.88106125723454 39.0 37.0 39.0 32.5 39.0 14-15 37.868340878268675 40.0 37.0 41.0 32.5 41.0 16-17 37.806559955176084 40.0 37.0 41.0 32.5 41.0 18-19 37.956805404338496 40.0 37.0 41.0 33.0 41.0 20-21 38.025272349881256 40.0 37.0 41.0 33.0 41.0 22-23 37.92335873779918 40.0 37.0 41.0 32.5 41.0 24-25 37.806175386319794 40.0 37.0 41.0 32.0 41.0 26-27 37.602773480029796 40.0 37.0 41.0 32.0 41.0 28-29 37.46211901260036 39.0 36.0 41.0 32.0 41.0 30-31 37.180922774244074 39.0 36.0 41.0 31.0 41.0 32-33 36.92052922786978 39.0 35.5 40.0 31.0 41.0 34-35 36.71907213212869 39.0 35.0 40.0 30.0 41.0 36-37 36.6719614921781 39.0 35.0 41.0 30.0 41.0 38-39 36.52534525242106 39.0 35.0 40.0 30.0 41.0 40-41 36.3147692905214 39.0 35.0 40.0 30.0 41.0 42-43 36.04739078944855 38.5 35.0 40.0 30.0 41.0 44-45 35.679497513673205 38.0 35.0 40.0 28.5 41.0 46-47 35.183437434339965 38.0 34.0 40.0 26.5 41.0 48-49 34.724553511737625 38.0 34.0 40.0 24.5 41.0 50-51 34.31320459190495 38.0 33.0 40.0 24.0 41.0 52-53 33.808239897108734 37.5 33.0 40.0 23.0 41.0 54-55 33.47904099733221 37.0 33.0 40.0 22.0 41.0 56-57 33.128699724307424 37.0 32.0 40.0 20.0 41.0 58-59 32.68254700462883 36.0 31.0 39.0 18.5 40.5 60-61 32.30650265186968 36.0 31.0 39.0 17.5 40.0 62-63 31.850635429997645 35.0 31.0 39.0 14.0 40.0 64-65 31.343975194035362 35.0 30.0 38.0 11.5 40.0 66-67 30.842307349467397 35.0 29.0 38.0 8.0 40.0 68-69 30.307338325088025 34.0 29.0 37.0 7.0 39.5 70-71 29.732494158246265 34.0 28.0 36.5 4.5 39.0 72-73 29.294314556949935 34.0 28.0 36.0 2.0 39.0 74-75 28.793193322254695 33.5 27.0 35.5 2.0 38.0 76-77 27.559391693567385 31.5 26.0 34.5 2.0 36.0 78-79 28.255268402320148 33.0 27.0 35.0 2.0 37.0 80-81 27.979365716068486 33.0 26.0 35.0 2.0 36.5 82-83 27.5159707498456 33.0 26.0 35.0 2.0 36.0 84-85 27.00579240922201 32.5 25.5 35.0 2.0 36.0 86-87 26.42971685799604 32.0 24.5 34.5 2.0 35.0 88-89 25.788992671543816 32.0 23.0 34.0 2.0 35.0 90-91 25.242664858428995 31.0 20.5 34.0 2.0 35.0 92-93 24.50307559579521 31.0 18.0 34.0 2.0 35.0 94-95 23.732860262703824 31.0 8.5 34.0 2.0 35.0 96-97 22.727495081466202 30.0 2.0 34.0 2.0 35.0 98-99 21.484198613259984 29.5 2.0 34.0 2.0 35.0 100-101 17.94847318523612 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38182710391110675 1101 2 0.49211858688568455 1101 3 0.36425569145580994 1101 4 0.23751296332520155 1101 5 0.10287895509426193 1101 6 0.17962979835912307 1101 7 0.30780435258787264 1101 8 0.3668183040498292 1101 9 0.2897677711565265 1101 10-11 0.34608859335342146 1101 12-13 0.396023298792052 1101 14-15 0.5044247861512261 1101 16-17 0.49328286616530903 1101 18-19 0.6095011680880447 1101 20-21 0.5863558508647557 1101 22-23 0.4463020613857225 1101 24-25 0.4090706671550919 1101 26-27 0.5284077103196765 1101 28-29 0.41492442894872283 1101 30-31 0.31801949370535 1101 32-33 0.21778929373817846 1101 34-35 0.16343369838737942 1101 36-37 0.43312473774278715 1101 38-39 0.602328697203518 1101 40-41 0.6630886328452803 1101 42-43 0.6513824146859903 1101 44-45 0.5991355586510068 1101 46-47 0.6566775275736276 1101 48-49 0.7488668062906925 1101 50-51 0.8454335101561199 1101 52-53 0.8563762556055181 1101 54-55 0.8662341023654392 1101 56-57 0.8415757877700543 1101 58-59 0.993704040571231 1101 60-61 0.89046671757486 1101 62-63 0.9619818597604279 1101 64-65 0.9098030589187616 1101 66-67 0.7267630770320181 1101 68-69 0.7441977173191212 1101 70-71 0.6691786096932972 1101 72-73 0.6796008579664132 1101 74-75 0.8311482278862208 1101 76-77 0.7976209151181912 1101 78-79 0.8422041065321544 1101 80-81 0.8950942347941861 1101 82-83 0.9106859145893047 1101 84-85 1.0486558876909875 1101 86-87 1.0503795780623086 1101 88-89 0.9029535898590559 1101 90-91 1.26816332438354 1101 92-93 1.1284948803744506 1101 94-95 1.3423061723272056 1101 96-97 1.5510935158460235 1101 98-99 1.4038636928143724 1101 100-101 1.024835574336599 1102 1 0.37080484416676995 1102 2 0.3870339102703788 1102 3 0.40056957670558546 1102 4 0.31922680253267544 1102 5 0.25677304698486125 1102 6 0.27135554778494964 1102 7 0.35263314892313957 1102 8 0.381764150231092 1102 9 0.4648812845710282 1102 10-11 0.536304063815372 1102 12-13 0.5161664295600943 1102 14-15 0.5708489189761892 1102 16-17 0.5330669918965896 1102 18-19 0.5862573008513863 1102 20-21 0.5647382425581569 1102 22-23 0.5840413845472909 1102 24-25 0.6024397852619217 1102 26-27 0.5973931020892636 1102 28-29 0.5592791061759783 1102 30-31 0.4245539198980097 1102 32-33 0.4814948911245054 1102 34-35 0.2723466297373207 1102 36-37 0.47559230518997 1102 38-39 0.5588243454570119 1102 40-41 0.5321134894418336 1102 42-43 0.5548551113117952 1102 44-45 0.6207371395753327 1102 46-47 0.5039757064050789 1102 48-49 0.44136753168584875 1102 50-51 0.3365245752443542 1102 52-53 0.2076846103676644 1102 54-55 0.21853844445389115 1102 56-57 0.28360079131897464 1102 58-59 0.4230978017149454 1102 60-61 0.3107839848104419 1102 62-63 0.303823027153328 1102 64-65 0.4470931574517749 1102 66-67 0.33289432089426185 1102 68-69 0.582743072105 1102 70-71 0.42528868492208716 1102 72-73 0.327543795490012 1102 74-75 0.30271315397917675 1102 76-77 0.3532259404726794 1102 78-79 0.5173080451468621 1102 80-81 0.5814235854270997 1102 82-83 0.4153879738789641 1102 84-85 0.2947043053720648 1102 86-87 0.13132514366030534 1102 88-89 0.12500339630239665 1102 90-91 0.0681184893427691 1102 92-93 0.23857697803824252 1102 94-95 0.2796316143603299 1102 96-97 0.3145474625160496 1102 98-99 0.40459803832893115 1102 100-101 0.09492248437802431 1103 1 0.31009890468454415 1103 2 0.3260596773649844 1103 3 0.2739460069462538 1103 4 0.21945442704328144 1103 5 0.1958485879724492 1103 6 0.09402209305187625 1103 7 0.18855290369567257 1103 8 0.21125943081623433 1103 9 0.29199227428239993 1103 10-11 0.3758081458815994 1103 12-13 0.4450826333902498 1103 14-15 0.5478442754361694 1103 16-17 0.4869927559562015 1103 18-19 0.4632411243361645 1103 20-21 0.4072348281905036 1103 22-23 0.4316482921924347 1103 24-25 0.29962088332255377 1103 26-27 0.4372377104233536 1103 28-29 0.3782271774376227 1103 30-31 0.47046244519467706 1103 32-33 0.49610474271332805 1103 34-35 0.45867034214133895 1103 36-37 0.618167193653953 1103 38-39 0.7085715305005635 1103 40-41 0.6992791090357571 1103 42-43 0.5432644712316659 1103 44-45 0.4897000823050135 1103 46-47 0.610814153702421 1103 48-49 0.7038622963221073 1103 50-51 0.7136868563454541 1103 52-53 0.8722814114845434 1103 54-55 0.8100550306932348 1103 56-57 0.8041428411535634 1103 58-59 0.8457613119884044 1103 60-61 0.9530401989595774 1103 62-63 0.7351204868637922 1103 64-65 0.7931006235357216 1103 66-67 0.8225170712053469 1103 68-69 0.7413876440191736 1103 70-71 0.581005095131804 1103 72-73 0.5154135335700722 1103 74-75 0.750094865108494 1103 76-77 0.6680427786164778 1103 78-79 0.7205579161596383 1103 80-81 0.7575576107389459 1103 82-83 0.5885240326364354 1103 84-85 0.5848496815595041 1103 86-87 0.6217606652239311 1103 88-89 0.48620221872492664 1103 90-91 0.6394365870088592 1103 92-93 0.6408696121090784 1103 94-95 0.5656846053463944 1103 96-97 0.47491651772258336 1103 98-99 0.2344350591793365 1103 100-101 -0.043810709345606824 1104 1 0.20026822338999395 1104 2 0.2246166937478158 1104 3 0.19595704648663315 1104 4 0.09799203109045607 1104 5 0.15590819756880592 1104 6 0.1291886175574959 1104 7 0.03995872935720968 1104 8 0.10121580208205927 1104 9 0.13350290694806688 1104 10-11 0.15839037869236705 1104 12-13 0.07331601280336741 1104 14-15 0.2816152687006763 1104 16-17 0.3451581921945106 1104 18-19 0.3632262219370759 1104 20-21 0.1810246455380664 1104 22-23 0.2377416086625317 1104 24-25 0.22885644907448466 1104 26-27 0.3760386555491877 1104 28-29 0.4315418827720947 1104 30-31 0.46020312384208495 1104 32-33 0.3713987219480046 1104 34-35 0.5093234005646252 1104 36-37 0.510878743993672 1104 38-39 0.38532123882531977 1104 40-41 0.5238051245068007 1104 42-43 0.49321594039252403 1104 44-45 0.3401968616220543 1104 46-47 0.3589816488248232 1104 48-49 0.47198959418380326 1104 50-51 0.6666182170717505 1104 52-53 0.6257408269575464 1104 54-55 0.6417349820643494 1104 56-57 0.5982689429016546 1104 58-59 0.49523610046257005 1104 60-61 0.2534107980948548 1104 62-63 0.2519056864284863 1104 64-65 0.38070320008209535 1104 66-67 0.34327023061199924 1104 68-69 0.21247953887882787 1104 70-71 0.043640270676185366 1104 72-73 -0.134290477332204 1104 74-75 0.05856138863369864 1104 76-77 -1.9942458557764553E-4 1104 78-79 -0.030671433723203023 1104 80-81 -0.10103940682039436 1104 82-83 -0.09815283040855505 1104 84-85 -0.05788490867354312 1104 86-87 0.015389105276089055 1104 88-89 -0.3177177004883802 1104 90-91 -0.21638838823551154 1104 92-93 -0.23068955139573077 1104 94-95 0.013487187775293563 1104 96-97 0.2938621729869517 1104 98-99 0.11899636980611916 1104 100-101 -0.20847868977640616 1105 1 0.23015836855065075 1105 2 0.1935837746171778 1105 3 0.1698382047115672 1105 4 0.10954218420052797 1105 5 -0.002463405505707783 1105 6 0.08285439535849548 1105 7 0.035546621625940134 1105 8 0.07150591813221041 1105 9 0.0562391452362192 1105 10-11 0.21181543500066624 1105 12-13 0.26165482923737216 1105 14-15 0.2233183079494765 1105 16-17 0.19945619047401664 1105 18-19 0.2333327178718747 1105 20-21 0.21148169914432202 1105 22-23 0.22677609154984424 1105 24-25 0.218772084588565 1105 26-27 0.24612947659608153 1105 28-29 0.2225737942313728 1105 30-31 0.32851260584302366 1105 32-33 0.4611428667242592 1105 34-35 0.3880109645937466 1105 36-37 0.31272912718869605 1105 38-39 0.30910190982248764 1105 40-41 0.2748711101866732 1105 42-43 0.26402407416649254 1105 44-45 0.2787462627107047 1105 46-47 0.0836602669714992 1105 48-49 0.09402774014314019 1105 50-51 -0.036523664922469834 1105 52-53 0.238060915632623 1105 54-55 0.16747314799050628 1105 56-57 0.1485978356387676 1105 58-59 0.28259150428686297 1105 60-61 0.0941278122526299 1105 62-63 0.3643709544224123 1105 64-65 0.07022621697551656 1105 66-67 0.015512803234312145 1105 68-69 -0.004728502338664953 1105 70-71 -0.01964708920618108 1105 72-73 0.13579717141594827 1105 74-75 0.10420890145817907 1105 76-77 0.14284736308873747 1105 78-79 0.22911016283877217 1105 80-81 0.16695769705746244 1105 82-83 0.1840968518950561 1105 84-85 0.2590740137898777 1105 86-87 0.248494468513627 1105 88-89 0.330781895510043 1105 90-91 0.3320697812734714 1105 92-93 0.5575636129722632 1105 94-95 0.8055004591803758 1105 96-97 0.4912834673221589 1105 98-99 0.4686891233427666 1105 100-101 0.28783174018203894 1106 1 0.07970669011432818 1106 2 0.21174688673574948 1106 3 0.25046924747444166 1106 4 0.10652252993338607 1106 5 0.09022534155810291 1106 6 0.007891459504676845 1106 7 0.08976819986770579 1106 8 0.09790529971179751 1106 9 0.014354979562284598 1106 10-11 0.06486067006391494 1106 12-13 0.15364150481686067 1106 14-15 0.2622494788030494 1106 16-17 0.050137535911851216 1106 18-19 0.2580461942833878 1106 20-21 0.2006234914431957 1106 22-23 0.27430334350015073 1106 24-25 0.2733762544450471 1106 26-27 0.2944478660142238 1106 28-29 0.2850019532729675 1106 30-31 0.39354757871243606 1106 32-33 0.3558269158842009 1106 34-35 0.15471503772867834 1106 36-37 -0.14320487062293097 1106 38-39 0.15196804196877167 1106 40-41 0.3460305364299501 1106 42-43 0.4821307965009609 1106 44-45 0.3297106773959584 1106 46-47 -0.018608021430509325 1106 48-49 0.023725737372195965 1106 50-51 0.0819406283940296 1106 52-53 0.0673115290115689 1106 54-55 -0.2642291139493764 1106 56-57 -0.12963234671309465 1106 58-59 -0.01885147667877618 1106 60-61 -0.5654394054903591 1106 62-63 -0.2674706014874957 1106 64-65 -0.6491031553800468 1106 66-67 -0.3732103602699226 1106 68-69 -1.1314210361071808 1106 70-71 -0.9626476605728698 1106 72-73 -0.6993570042288901 1106 74-75 -0.5462584615538546 1106 76-77 -0.5390804579875983 1106 78-79 -0.5595554905545086 1106 80-81 -1.4138232827803918 1106 82-83 -0.9192560996059953 1106 84-85 -0.8956573131625731 1106 86-87 -0.5170719127186558 1106 88-89 -0.43700534695482673 1106 90-91 -0.31028342790274976 1106 92-93 -0.5481521078273524 1106 94-95 -0.23137584553358437 1106 96-97 -0.2206315820282363 1106 98-99 -0.3144818870800066 1106 100-101 -0.6214695866583781 1107 1 0.08706505864712 1107 2 0.03962117347713345 1107 3 0.09056745727450277 1107 4 0.052498300134828924 1107 5 -0.10141194516413066 1107 6 0.1233896318830574 1107 7 0.04775868533291572 1107 8 0.03746560975185531 1107 9 0.12164321928495525 1107 10-11 0.06514427922250121 1107 12-13 0.03545420196206095 1107 14-15 0.18012363294794653 1107 16-17 0.1221319049809253 1107 18-19 0.15476126357978615 1107 20-21 0.11520520108340548 1107 22-23 0.20868539229270766 1107 24-25 0.17167548347012485 1107 26-27 0.2248586499393994 1107 28-29 0.1559130090209564 1107 30-31 0.1929633438457543 1107 32-33 0.31777282830274345 1107 34-35 0.33776412347587836 1107 36-37 0.1837810196372729 1107 38-39 -0.027752135239069275 1107 40-41 0.08561545048532793 1107 42-43 0.10239949592092046 1107 44-45 0.28866653897110695 1107 46-47 0.16468194793970525 1107 48-49 0.20182529081668577 1107 50-51 0.09658959392042021 1107 52-53 0.2981351300081059 1107 54-55 0.5285910807906617 1107 56-57 0.41347329880345285 1107 58-59 0.1885187518733673 1107 60-61 0.054708852118103835 1107 62-63 0.13998625677128018 1107 64-65 0.3770526071897322 1107 66-67 0.43633945991450673 1107 68-69 0.27471832869669655 1107 70-71 0.16864533199730758 1107 72-73 0.16242036331826526 1107 74-75 0.1177464533560304 1107 76-77 -0.10230835746767397 1107 78-79 -0.08500407184573433 1107 80-81 0.020816592519977917 1107 82-83 0.1135040731230319 1107 84-85 0.10446865350267132 1107 86-87 0.11180966535636472 1107 88-89 0.051434449082307765 1107 90-91 -0.09337988006267395 1107 92-93 -0.15940792918157243 1107 94-95 -0.5004491139339251 1107 96-97 -0.20369274878894927 1107 98-99 0.11941434290713104 1107 100-101 -0.42931480530262434 1108 1 0.1112496745590974 1108 2 0.09050488264901091 1108 3 0.09946989255402627 1108 4 0.006250056019858619 1108 5 0.0023689832286137857 1108 6 -0.012848478623972426 1108 7 0.018037720190669404 1108 8 -0.044488426944802484 1108 9 0.10777931528214424 1108 10-11 0.013125805919450784 1108 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2316 70-71 -0.8911414219294329 2316 72-73 -0.5973322073879217 2316 74-75 -0.5245381904721285 2316 76-77 -0.28557051340490247 2316 78-79 -0.3284847895728973 2316 80-81 -0.506304856547402 2316 82-83 -0.4895762190456239 2316 84-85 -0.48464865894325726 2316 86-87 -0.1689377274201327 2316 88-89 0.04945423168003771 2316 90-91 -0.23736013209992635 2316 92-93 -0.30463711111084635 2316 94-95 -0.20052542383267635 2316 96-97 -0.20059912968188343 2316 98-99 -0.29176895369555567 2316 100-101 -0.027110251103771077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 27.0 5 72.0 6 143.0 7 429.0 8 920.0 9 1617.0 10 2623.0 11 4055.0 12 5663.0 13 8015.0 14 10425.0 15 13894.0 16 17977.0 17 22916.0 18 20573.0 19 15268.0 20 14173.0 21 14833.0 22 16146.0 23 17904.0 24 20928.0 25 24694.0 26 29276.0 27 34582.0 28 42056.0 29 50297.0 30 59067.0 31 70118.0 32 83868.0 33 103118.0 34 130888.0 35 170130.0 36 217760.0 37 236546.0 38 105912.0 39 3665.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.17888932750959 19.31152822524163 15.345159113193851 22.164423334054938 2 16.134007123077893 21.205075463729443 38.62865143738768 24.03226597580498 3 19.21892648712388 25.26159092616622 30.04911555079582 25.470367035914077 4 13.333078651971553 17.535193780681148 35.52384772601379 33.607879841333514 5 13.760167627243506 37.74200291104489 33.01040787766474 15.487421584046862 6 31.89260086973685 36.07739766584532 16.837621530762327 15.192379933655506 7 28.89672034076366 33.496647756575555 19.554180276201937 18.052451626458847 8 27.419632112772906 34.147422306267075 19.086457955290687 19.346487625669333 9 27.355961772327596 16.563839066847493 19.522599787341065 36.55759937348385 10-11 25.53448067286816 26.912752532487794 26.067815279608297 21.48495151503575 12-13 26.712955004170407 24.29475547405752 27.003387262111687 21.98890225966038 14-15 23.220764171426026 25.231187006156926 26.10700437415239 25.441044448264666 16-17 22.09399015656537 28.859568697113826 26.53735220522224 22.509088941098568 18-19 22.1267803818947 26.34923818437657 30.102127226074277 21.421854207654448 20-21 22.905086623498175 25.335033331423222 30.25547724103681 21.504402804041796 22-23 22.603161869106515 25.426081918260017 30.14688747540733 21.82386873722614 24-25 22.424276767681913 25.75032408244556 30.17529103749678 21.650108112375747 26-27 21.794660356765803 26.190588382124268 30.13177181965332 21.88297944145661 28-29 21.68707301077939 26.244086617131142 30.41181976200027 21.6570206100892 30-31 21.669627337497374 25.94314875301638 30.23666265543522 22.150561254051027 32-33 21.60159557873156 26.106081154215932 30.006271528533862 22.286051738518644 34-35 22.42355721273441 26.132628717469014 29.670152282227562 21.773661787569015 36-37 22.45739577400715 25.680039246410345 29.695601555976275 22.16696342360623 38-39 21.855188681197525 26.085381302174216 29.863462376621243 22.195967640007016 40-41 22.181106420803868 25.879459139195788 29.848904470656258 22.09052996934409 42-43 22.158993775243406 25.98168133053734 29.482545818162485 22.376779076056767 44-45 21.978396653486907 26.265607192201657 29.368549398633636 22.387446755677804 46-47 22.237248422567315 26.623147988972296 28.689218701252393 22.450384887207992 48-49 22.455255668252057 26.569346551296007 28.44673020966643 22.5286675707855 50-51 22.461017834062357 26.836475464634308 28.10517066834756 22.597336032955766 52-53 22.85182638371567 26.822149638034116 27.863605396698055 22.462418581552154 54-55 22.214581781368786 26.906703850145487 28.076996542700517 22.801717825785214 56-57 22.27468658274916 27.165078091672555 28.04204152579604 22.518193799782246 58-59 22.427368059137013 26.466073259093715 28.08053024659523 23.02602843517404 60-61 22.196244723320536 26.826797572886623 28.353930688467393 22.62302701532545 62-63 22.027645661821353 27.008703735538873 28.25654690275629 22.707103699883483 64-65 21.88445106615985 26.774810739913026 28.131784870653703 23.20895332327342 66-67 21.908755250418793 27.04800555715085 27.8999470899134 23.143292102516956 68-69 22.044834620762373 26.73965281438864 28.0167353040803 23.198777260768686 70-71 21.92958551776216 27.07081942663439 27.72719863796061 23.27239641764284 72-73 22.245143544782533 26.920583984362562 27.68465990487651 23.14961256597839 74-75 21.9401626140495 27.207196021877127 27.485435409623136 23.367205954450238 76-77 21.914216950318032 26.915904214339836 27.94099669550933 23.228882139832802 78-79 22.079091296901165 26.98791536938348 27.768418237732316 23.16457509598304 80-81 21.905241864880644 27.358750056508253 27.620216138502844 23.115791940108256 82-83 21.984588471438055 27.20527958664886 27.46750797079418 23.342623971118904 84-85 21.836299308535175 26.963657902721195 27.560821938654822 23.63922085008881 86-87 22.061012248229684 26.90452760762368 27.487379449347 23.547080694799636 88-89 21.841876468597597 26.993639645266725 27.373974787157806 23.790509098977868 90-91 21.634163401058686 26.955154439459942 27.677838341023392 23.732843818457983 92-93 21.830094073826466 26.622351686484247 27.611129681804435 23.93642455788485 94-95 21.59354815500804 26.544937426361248 27.621889742673055 24.239624675957657 96-97 21.810561636073068 26.429685659529223 27.502912918075374 24.256839786322338 98-99 21.496464541794367 26.528622869707757 27.26840926187859 24.706503326619288 100-101 21.453180015153542 26.3579928561878 27.703283479456765 24.485543649201894 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1501.0 1 978.5 2 616.0 3 810.5 4 722.5 5 672.0 6 1169.5 7 2566.5 8 3873.5 9 4100.0 10 3474.5 11 2828.5 12 2707.5 13 2828.5 14 3033.5 15 3151.0 16 3227.0 17 3372.5 18 3578.5 19 3845.5 20 4152.0 21 4554.0 22 5119.5 23 5835.5 24 6643.5 25 7679.5 26 9134.0 27 10838.5 28 12226.5 29 13841.5 30 16078.5 31 18605.0 32 21728.0 33 23719.0 34 25254.0 35 27118.0 36 29245.0 37 32132.0 38 34503.5 39 35823.0 40 37495.0 41 41899.5 42 46813.0 43 50749.5 44 53657.0 45 56034.5 46 56851.5 47 58330.0 48 62944.5 49 64222.0 50 63147.5 51 61500.0 52 58495.0 53 57412.0 54 57405.5 55 53631.0 56 47220.0 57 41920.0 58 38237.0 59 34457.0 60 28817.0 61 23428.5 62 19113.0 63 14589.5 64 11200.0 65 9022.0 66 6692.0 67 5186.5 68 3996.0 69 2821.5 70 2178.0 71 1806.0 72 1624.5 73 1438.5 74 1168.0 75 1016.0 76 716.0 77 369.5 78 206.5 79 113.5 80 58.0 81 35.5 82 14.5 83 8.5 84 5.5 85 4.0 86 2.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.367034044531036E-4 2 0.0485804697597718 3 5.093627235624829E-4 4 0.0 5 0.08875645458076264 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2734068089062072E-4 26-27 0.009423210385905934 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011683507471714451 36-37 3.183517022265518E-5 38-39 0.00448875900139438 40-41 0.005953176831636519 42-43 4.138572128945174E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.367034044531036E-5 68-69 0.0011460661280155865 70-71 2.5468136178124145E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014644178302421383 82-83 7.958792555663796E-4 84-85 0.005316473427183415 86-87 0.002323967426253828 88-89 9.550551066796554E-5 90-91 0.003533703894714725 92-93 9.550551066796555E-4 94-95 3.183517022265518E-5 96-97 0.0 98-99 9.550551066796554E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1570590.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.08261129283729 #Duplication Level Percentage of deduplicated Percentage of total 1 73.70702221589792 33.96612054327747 2 13.612547535250345 12.546034735444236 3 4.903711154938357 6.779274451361235 4 2.404130284871166 4.431544056602245 5 1.3378370238094344 3.082551177068823 6 0.8494617520742695 2.3487249437382647 7 0.5684505700902514 1.8336980662462568 8 0.398194504394161 1.4679874051952086 9 0.2808628519247334 1.1648604268660687 >10 1.670951702927866 14.380769849092603 >50 0.16305407316721918 5.246002563283504 >100 0.09602777172818058 8.34550309923037 >500 0.0059497863182804405 1.8472194336131602 >1k 0.0013836712368094047 0.9450979097347 >5k 2.767342473618809E-4 0.9501352012519744 >10k+ 1.3836712368094046E-4 0.6644761379938813 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10421 0.6635086177805792 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8488 0.5404338496997944 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6413 0.4083178932757754 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2117 0.134790110722722 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2025 0.12893243940175347 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1736 0.11053171101305878 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1580 0.10059913790359037 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.367034044531036E-5 0.0 14-15 0.0 0.0 0.0 6.367034044531036E-5 0.0 16-17 0.0 0.0 0.0 6.367034044531036E-5 0.0 18-19 0.0 0.0 0.0 6.367034044531036E-5 0.0 20-21 0.0 0.0 0.0 1.2734068089062072E-4 0.0 22-23 0.0 0.0 0.0 4.456923831171725E-4 0.0 24-25 0.0 0.0 0.0 0.0011779012982382417 0.0 26-27 0.0 0.0 0.0 0.002674154298703035 0.0 28-29 0.0 0.0 0.0 0.007226583640542726 0.0 30-31 0.0 0.0 0.0 0.019992486899827452 0.0 32-33 0.0 0.0 0.0 0.04351867769436964 0.0 34-35 0.0 0.0 0.0 0.07850552976906768 0.0 36-37 0.0 0.0 0.0 0.13873767183033126 0.0 38-39 0.0 0.0 0.0 0.23102783030580865 0.0 40-41 0.0 0.0 0.0 0.33102209997516857 0.0 42-43 0.0 0.0 0.0 0.431525732368091 0.0 44-45 0.0 0.0 0.0 0.5563832699813447 0.0 46-47 0.0 0.0 0.0 0.702220184771328 0.0 48-49 0.0 0.0 0.0 0.8583080243730064 0.0 50-51 0.0 0.0 0.0 1.0291992181282192 0.0 52-53 0.0 0.0 0.0 1.1993263677980885 0.0 54-55 0.0 0.0 0.0 1.3804684863649965 0.0 56-57 0.0 0.0 0.0 1.5943371599207943 0.0 58-59 0.0 0.0 0.0 1.8283256610573098 0.0 60-61 0.0 0.0 0.0 2.0765126481131295 0.0 62-63 0.0 0.0 0.0 2.3238719207431604 0.0 64-65 0.0 0.0 0.0 2.5824690084617883 0.0 66-67 0.0 0.0 0.0 2.875861937233778 0.0 68-69 0.0 0.0 0.0 3.2036686850164586 0.0 70-71 0.0 0.0 0.0 3.5606046135528686 0.0 72-73 0.0 0.0 0.0 3.925085477432048 0.0 74-75 0.0 0.0 0.0 4.292718023163269 6.367034044531036E-5 76-77 0.0 0.0 0.0 4.686805595349519 6.367034044531036E-5 78-79 0.0 0.0 0.0 5.116612228525586 6.367034044531036E-5 80-81 0.0 0.0 0.0 5.586149154139527 6.367034044531036E-5 82-83 0.0 0.0 0.0 6.0726223903119205 6.367034044531036E-5 84-85 0.0 0.0 0.0 6.55352447169535 6.367034044531036E-5 86-87 0.0 0.0 0.0 7.047574478380736 6.367034044531036E-5 88-89 0.0 0.0 0.0 7.566742434371797 6.367034044531036E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4795 0.0 55.25065 1 GTATCAA 8920 0.0 42.124336 1 TATCAAC 11765 0.0 31.9783 2 ATCAACG 11835 0.0 31.653456 3 TCAACGC 12025 0.0 31.113783 4 CAACGCA 12345 0.0 30.30727 5 AACGCAG 12510 0.0 29.916937 6 GCGTATC 65 0.005806877 29.269646 1 TGGTATC 2125 0.0 27.530684 2 GTGGTAT 2295 0.0 26.113113 1 ACGCAGA 14505 0.0 25.703964 7 CGCAGAG 14575 0.0 25.51534 8 GTACATG 9315 0.0 24.968752 1 TACATGG 9630 0.0 24.003843 2 GCAGAGT 15410 0.0 23.670464 9 GAGTACT 8625 0.0 22.715015 12-13 ACATGGG 9840 0.0 22.562384 3 AGTACTT 8735 0.0 22.102728 12-13 GGACCGA 560 0.0 22.051266 6 GTACTTT 9065 0.0 21.717255 14-15 >>END_MODULE