FastQCFastQC Report
Thu 26 May 2016
SRR938649_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938649_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578555
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110420.6995004925390627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82810.5245936948665076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60770.38497233229124106No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.1299289540117386No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17030.1078834757103807No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG16170.10243545521061984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.054.6539461
GTATCAA89550.042.027061
TATCAAC117450.031.8045462
ATCAACG118850.031.3899153
TCAACGC120550.030.9472544
CAACGCA122700.030.4049845
TATTACG404.5025622E-429.67943270-71
AACGCAG126750.029.5825046
GTACATG97100.025.8558541
ACGCAGA145350.025.5304997
CGCAGAG146350.025.3235848
TATAACG950.001223040325.0130182
TACATGG99950.024.820322
GCAGAGT150100.024.2145569
TGGTATC21300.023.8739262
ACCGAGT5450.023.5391398
ACATGGG102250.023.4253943
GAGTACT81600.023.31594812-13
GGACCGA5050.022.5852976
TAAGGTG5950.022.364585
Produced by FastQC (version 0.11.5)