##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938649_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1578555 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.196002039840234 31.0 31.0 34.0 28.0 34.0 2 31.30122675484858 31.0 31.0 34.0 28.0 34.0 3 31.343940502548218 31.0 31.0 34.0 28.0 34.0 4 35.042552841047666 37.0 35.0 37.0 32.0 37.0 5 35.05409250865507 37.0 35.0 37.0 32.0 37.0 6 35.079814767303006 37.0 35.0 37.0 32.0 37.0 7 35.00420321116464 37.0 35.0 37.0 32.0 37.0 8 34.967804732809434 37.0 35.0 37.0 32.0 37.0 9 36.44712284335991 39.0 37.0 39.0 32.0 39.0 10-11 36.30730889959489 39.0 35.0 39.0 32.0 39.0 12-13 36.132516447003745 38.5 35.0 39.0 31.0 39.0 14-15 37.086722667249475 39.0 36.0 41.0 31.0 41.0 16-17 36.94174038915337 39.0 36.0 41.0 31.0 41.0 18-19 36.86103873479226 39.0 36.0 41.0 31.0 41.0 20-21 36.877673251803074 39.0 36.0 40.5 30.5 41.0 22-23 36.95707593336944 39.0 36.0 41.0 31.0 41.0 24-25 36.859516773251485 39.0 36.0 41.0 30.0 41.0 26-27 36.66731219374681 39.0 36.0 41.0 30.0 41.0 28-29 36.46948475029378 39.0 35.5 40.5 30.0 41.0 30-31 36.17280740930788 38.5 35.0 40.0 29.5 41.0 32-33 35.83574059820532 38.0 35.0 40.0 28.5 41.0 34-35 35.547749048972 38.0 34.5 40.0 27.0 41.0 36-37 35.23403207363697 38.0 34.0 40.0 26.5 41.0 38-39 34.90084666039511 38.0 34.0 40.0 25.0 41.0 40-41 34.745054496042265 38.0 33.5 40.0 25.0 41.0 42-43 34.452291811181745 38.0 33.0 40.0 24.0 41.0 44-45 34.05989560072345 38.0 33.0 40.0 23.5 41.0 46-47 33.547117141943104 38.0 33.0 40.0 20.0 41.0 48-49 33.07899281304738 37.0 32.0 40.0 16.5 41.0 50-51 32.25546084868756 36.5 30.5 39.5 14.5 40.5 52-53 32.26260409044981 36.5 31.0 39.0 13.0 40.0 54-55 32.3288634225605 37.0 31.0 40.0 9.0 41.0 56-57 31.994699899591716 36.0 31.0 39.5 8.0 41.0 58-59 31.55146890668998 36.0 30.0 39.0 4.5 41.0 60-61 31.071902784508616 35.0 29.5 39.0 2.0 40.5 62-63 30.491395611809537 35.0 28.5 38.5 2.0 40.0 64-65 29.99202403463927 35.0 28.0 38.0 2.0 40.0 66-67 29.43086335287652 34.0 27.5 38.0 2.0 40.0 68-69 28.813915891432355 34.0 26.0 37.0 2.0 39.5 70-71 28.174294845602468 34.0 26.0 36.5 2.0 39.0 72-73 27.53526674711999 33.0 25.0 36.0 2.0 39.0 74-75 26.84288447345832 33.0 23.5 35.5 2.0 38.0 76-77 26.11087418556845 32.0 21.0 35.0 2.0 37.0 78-79 25.32502446858044 31.5 18.5 35.0 2.0 37.0 80-81 24.56492805128741 31.0 13.0 35.0 2.0 36.0 82-83 23.840271957581457 31.0 7.0 34.0 2.0 36.0 84-85 23.10033226590141 30.0 2.0 34.0 2.0 35.0 86-87 22.364684790837188 29.5 2.0 34.0 2.0 35.0 88-89 21.590521077821172 29.0 2.0 34.0 2.0 35.0 90-91 20.761538875743955 28.5 2.0 34.0 2.0 35.0 92-93 19.88639008460269 26.5 2.0 34.0 2.0 35.0 94-95 18.983035751050803 25.5 2.0 33.0 2.0 35.0 96-97 17.905500916977868 24.0 2.0 33.0 2.0 35.0 98-99 16.662077342886377 10.5 2.0 32.5 2.0 34.5 100-101 13.941749891514707 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7078070592864556 1101 2 0.7195323928921518 1101 3 0.7157746275306316 1101 4 0.5565831703030284 1101 5 0.5852038564990778 1101 6 0.49841972173940263 1101 7 0.486260365392603 1101 8 0.37372677867575277 1101 9 0.6977931782868225 1101 10-11 0.6412281851504602 1101 12-13 0.7935100746880224 1101 14-15 0.8481632329839996 1101 16-17 0.9305565955603825 1101 18-19 1.0540276741983021 1101 20-21 1.0542573288077435 1101 22-23 0.8275472312340426 1101 24-25 0.9508386634623065 1101 26-27 0.9470664350015383 1101 28-29 1.0401002560876904 1101 30-31 0.9484823400452882 1101 32-33 1.0706909859754106 1101 34-35 1.0281444361829273 1101 36-37 1.029099723270832 1101 38-39 1.1690748633964745 1101 40-41 1.3487487405874745 1101 42-43 1.3643890106674164 1101 44-45 1.3170386785600954 1101 46-47 1.6667830855997323 1101 48-49 1.5634300071528955 1101 50-51 1.4097364857872279 1101 52-53 1.84322818971021 1101 54-55 1.9262551741546616 1101 56-57 1.984812152625267 1101 58-59 2.1467195920600908 1101 60-61 2.35985261658632 1101 62-63 2.2882182392961 1101 64-65 2.307647843158769 1101 66-67 2.4015011005700657 1101 68-69 2.479498203729282 1101 70-71 2.5966586517591566 1101 72-73 2.8791210258974402 1101 74-75 3.0102944740717845 1101 76-77 3.2082094402064136 1101 78-79 3.5715254405990677 1101 80-81 3.950729896894238 1101 82-83 4.110906794547326 1101 84-85 4.218685956749805 1101 86-87 4.243120326975326 1101 88-89 4.809729282811432 1101 90-91 5.073426219371392 1101 92-93 4.836188222662972 1101 94-95 5.096468751468919 1101 96-97 5.168406856711339 1101 98-99 5.649604559406715 1101 100-101 5.482979200530901 1102 1 0.6080878813744803 1102 2 0.6124323158524625 1102 3 0.5801922323418793 1102 4 0.5027871018122596 1102 5 0.4675145501047808 1102 6 0.48125975255527464 1102 7 0.4970931395669069 1102 8 0.5898032219273261 1102 9 0.7209870946366337 1102 10-11 0.5671408569365397 1102 12-13 0.6289520588327591 1102 14-15 0.914353993191753 1102 16-17 0.8958850082943073 1102 18-19 1.009993279705398 1102 20-21 0.8959159187329391 1102 22-23 0.7956490727311021 1102 24-25 0.7782921290056848 1102 26-27 0.8449938188828483 1102 28-29 1.0016885162333224 1102 30-31 1.0144320405845662 1102 32-33 0.9095798748642991 1102 34-35 1.0004573664247332 1102 36-37 1.2522203276596073 1102 38-39 1.3658888995305745 1102 40-41 1.3548038115385381 1102 42-43 1.5590285394827461 1102 44-45 1.643137736687791 1102 46-47 1.563926404270667 1102 48-49 1.4462414587297232 1102 50-51 1.4910854258702315 1102 52-53 1.649641122437167 1102 54-55 1.9012060924180432 1102 56-57 1.978857730298671 1102 58-59 2.1167747624172044 1102 60-61 2.1144476611947844 1102 62-63 2.3307168103341382 1102 64-65 2.338708505700083 1102 66-67 2.4098338126155916 1102 68-69 2.552273372098277 1102 70-71 2.6611296873216688 1102 72-73 2.9517657238273074 1102 74-75 2.948171906413542 1102 76-77 3.0447274948300382 1102 78-79 3.529154854848926 1102 80-81 3.940764440496217 1102 82-83 4.116535662063914 1102 84-85 3.989174785995065 1102 86-87 4.237779737000427 1102 88-89 4.656468764408885 1102 90-91 4.918488223894368 1102 92-93 4.680621483926917 1102 94-95 5.217009768889827 1102 96-97 5.247065620597123 1102 98-99 5.60964332776577 1102 100-101 5.6248366025538115 1103 1 0.5129245672825604 1103 2 0.5615710523275581 1103 3 0.5554631270439074 1103 4 0.39794042242373706 1103 5 0.3752469659414359 1103 6 0.4303485216281473 1103 7 0.5176329144122107 1103 8 0.3670239557025923 1103 9 0.5200293067807706 1103 10-11 0.5200628395796087 1103 12-13 0.6858268595579986 1103 14-15 0.8479615920439372 1103 16-17 0.9550872588895416 1103 18-19 1.0296082610429664 1103 20-21 1.0845730011759827 1103 22-23 0.7908763926858668 1103 24-25 0.8991212389038381 1103 26-27 0.7736621796823897 1103 28-29 0.9990628482167381 1103 30-31 1.0519797673850206 1103 32-33 1.2518827534625458 1103 34-35 1.0643494146282038 1103 36-37 1.2556815616799497 1103 38-39 1.4058152333033007 1103 40-41 1.4520514801229965 1103 42-43 1.6669755772089303 1103 44-45 1.5788729158010923 1103 46-47 1.6240352063061678 1103 48-49 1.5816194105738433 1103 50-51 1.6267925280622961 1103 52-53 1.8498684344605891 1103 54-55 1.9523572461891519 1103 56-57 2.031446834866962 1103 58-59 2.276214794396349 1103 60-61 2.4198373196874208 1103 62-63 2.5841645610872206 1103 64-65 2.715435355326761 1103 66-67 2.6244116832428546 1103 68-69 2.9777390603556206 1103 70-71 3.0986402954804753 1103 72-73 3.2061061461866913 1103 74-75 3.354057511201532 1103 76-77 3.5362722965546247 1103 78-79 3.6704963764911582 1103 80-81 3.976421733912737 1103 82-83 4.082850891334978 1103 84-85 4.441471382977031 1103 86-87 4.3753550648421005 1103 88-89 4.628363686927685 1103 90-91 5.080942559240672 1103 92-93 4.8168028798733715 1103 94-95 5.124948795968987 1103 96-97 5.253075192130616 1103 98-99 5.2144773170204 1103 100-101 5.316284721320775 1104 1 0.5389044105076444 1104 2 0.5580600846706183 1104 3 0.4772469357521629 1104 4 0.42259624219845193 1104 5 0.33914262498892356 1104 6 0.47382391850582195 1104 7 0.41113136642459125 1104 8 0.5555293242553887 1104 9 0.6065651081113828 1104 10-11 0.5607156292509075 1104 12-13 0.624332228110795 1104 14-15 0.7266290052578128 1104 16-17 0.7712686698637938 1104 18-19 0.8538556755742874 1104 20-21 0.8505765582539055 1104 22-23 0.8999930516674866 1104 24-25 0.9638073597127388 1104 26-27 0.8246206145681043 1104 28-29 0.8534301494485419 1104 30-31 0.9442167741698171 1104 32-33 1.1298585126351952 1104 34-35 1.1858499745386197 1104 36-37 1.3550887153588889 1104 38-39 1.528448788405072 1104 40-41 1.7793816955238313 1104 42-43 1.7649566061266526 1104 44-45 1.869291860534645 1104 46-47 2.115613494956449 1104 48-49 2.180819068040414 1104 50-51 1.9799807238333287 1104 52-53 1.9795370992389323 1104 54-55 2.259003712166354 1104 56-57 2.456965575397888 1104 58-59 2.6376896642995113 1104 60-61 2.67096372769743 1104 62-63 2.8778639221306292 1104 64-65 2.7174345648649947 1104 66-67 2.797717130841825 1104 68-69 3.1761614304234698 1104 70-71 3.2976390439160213 1104 72-73 3.3090143867505546 1104 74-75 3.430366713493868 1104 76-77 3.6364172145442204 1104 78-79 3.9132867065089414 1104 80-81 4.106113453825785 1104 82-83 4.272413502493663 1104 84-85 4.508555237795555 1104 86-87 4.713639762819838 1104 88-89 5.1121888631347865 1104 90-91 5.7651530855564594 1104 92-93 5.6784654844448745 1104 94-95 5.9389696114620385 1104 96-97 5.994877444946635 1104 98-99 5.969315601718378 1104 100-101 5.735782778102278 1105 1 0.38284499646010417 1105 2 0.46766062651344953 1105 3 0.394129528296542 1105 4 0.3673716660791584 1105 5 0.339606360949972 1105 6 0.4131477092171423 1105 7 0.4403844647581252 1105 8 0.39988610285637094 1105 9 0.5784756889510518 1105 10-11 0.5675816759678725 1105 12-13 0.5758689902225527 1105 14-15 0.739887552205893 1105 16-17 0.7187255743036189 1105 18-19 0.8597104835804359 1105 20-21 0.8481360015443613 1105 22-23 0.7760578237708771 1105 24-25 0.6474679306475508 1105 26-27 0.8181583086797133 1105 28-29 0.9593108044142653 1105 30-31 1.0030999213861733 1105 32-33 1.0081478212384596 1105 34-35 1.0577933829036041 1105 36-37 1.106591329297764 1105 38-39 1.119378958221489 1105 40-41 1.1846731530093564 1105 42-43 1.2948134290636588 1105 44-45 1.6140610585053707 1105 46-47 1.3535636235309312 1105 48-49 1.2483907556204699 1105 50-51 1.2191377356229296 1105 52-53 1.3964627071613123 1105 54-55 1.6948408046286758 1105 56-57 1.9075858080959875 1105 58-59 1.8045555600853298 1105 60-61 2.090858340021338 1105 62-63 2.0585686951396127 1105 64-65 2.1070844611941624 1105 66-67 2.134115121193549 1105 68-69 2.3797515165108223 1105 70-71 2.6710304480096525 1105 72-73 2.621920132133475 1105 74-75 2.693620638175453 1105 76-77 2.947143136780717 1105 78-79 3.329243713770616 1105 80-81 3.5323933971511394 1105 82-83 3.98162766059027 1105 84-85 4.1577003071578496 1105 86-87 4.2909259452616375 1105 88-89 4.561680508152268 1105 90-91 4.6873160044139865 1105 92-93 4.991146342413 1105 94-95 5.1551375450070545 1105 96-97 5.379180416592334 1105 98-99 5.727408624359935 1105 100-101 5.3317180135454425 1106 1 0.3436160085071691 1106 2 0.48692505252010676 1106 3 0.30049056518774364 1106 4 0.31338357251519255 1106 5 0.36379611391485867 1106 6 0.050683170708730074 1106 7 0.37345191893709284 1106 8 0.3154321582634907 1106 9 0.3619983064919481 1106 10-11 0.27329957781009284 1106 12-13 0.2046332521641432 1106 14-15 0.6729857568653443 1106 16-17 0.5191448308545077 1106 18-19 0.6909225962696937 1106 20-21 0.6254629597780763 1106 22-23 0.5242350140394834 1106 24-25 0.6543841637136936 1106 26-27 0.6744420006123946 1106 28-29 0.4735130263239924 1106 30-31 0.7314184888637882 1106 32-33 0.8285029517873781 1106 34-35 0.9159704794207357 1106 36-37 0.9195421566544439 1106 38-39 0.741541429510093 1106 40-41 0.93381560836022 1106 42-43 0.7669199307277452 1106 44-45 1.032455470918066 1106 46-47 1.2899273792146104 1106 48-49 1.4464013495360106 1106 50-51 0.8472367371698297 1106 52-53 1.3051879684935201 1106 54-55 1.2897986633452163 1106 56-57 1.2415733391511594 1106 58-59 0.6271871637041322 1106 60-61 1.81615829783318 1106 62-63 2.2629613262744783 1106 64-65 2.3297222524096526 1106 66-67 2.3043749065026944 1106 68-69 1.7499818638600004 1106 70-71 2.3251503561332143 1106 72-73 2.601719316495732 1106 74-75 2.790816344358362 1106 76-77 2.830310998778561 1106 78-79 3.2261116648323522 1106 80-81 3.573859107552863 1106 82-83 3.949814788514143 1106 84-85 4.216826731008226 1106 86-87 4.464509970012028 1106 88-89 4.214180765968795 1106 90-91 4.601051156525738 1106 92-93 4.932668866202437 1106 94-95 4.654338032514669 1106 96-97 4.967384232276949 1106 98-99 4.986032915364483 1106 100-101 4.715387445880319 1107 1 0.45720180732373805 1107 2 0.42524457914732494 1107 3 0.27563849418182684 1107 4 0.358377655355433 1107 5 0.3411925636190034 1107 6 0.3548725198211571 1107 7 0.31721523254656603 1107 8 0.2373729866658536 1107 9 0.2798444603381043 1107 10-11 0.47720490325387743 1107 12-13 0.43153266044816974 1107 14-15 0.6474709639659366 1107 16-17 0.8322099196118984 1107 18-19 0.6165794010034205 1107 20-21 0.5258298236834094 1107 22-23 0.7141640081223173 1107 24-25 0.7425971814651717 1107 26-27 0.7643828290147638 1107 28-29 0.7813946831287168 1107 30-31 0.8650871279170431 1107 32-33 0.953958573089146 1107 34-35 0.8289527279414486 1107 36-37 0.847707837127814 1107 38-39 0.9338136188592117 1107 40-41 1.1240049574726427 1107 42-43 1.2112157887159114 1107 44-45 1.3366329857109633 1107 46-47 1.1865901011081021 1107 48-49 1.3420818229087885 1107 50-51 1.396952713501193 1107 52-53 1.468513412280501 1107 54-55 1.6812306160079302 1107 56-57 1.8048396113405119 1107 58-59 1.9212700039408226 1107 60-61 2.011531078898269 1107 62-63 2.195541207931278 1107 64-65 2.200574323415566 1107 66-67 2.2799015337216275 1107 68-69 2.540916775102602 1107 70-71 2.6359787585000802 1107 72-73 2.9895418017028312 1107 74-75 3.0389346875536276 1107 76-77 3.4512577443406904 1107 78-79 3.5437921382051307 1107 80-81 3.8387466815173568 1107 82-83 4.077637273721244 1107 84-85 4.2213119381088156 1107 86-87 4.456379792497234 1107 88-89 4.470488458276485 1107 90-91 4.849773050016861 1107 92-93 4.9826688662024345 1107 94-95 4.868586553224727 1107 96-97 5.216283640560974 1107 98-99 5.191500370985786 1107 100-101 4.7392927713241075 1108 1 0.4930051972019456 1108 2 0.5981417359555756 1108 3 0.5574282767033374 1108 4 0.6116848621886604 1108 5 0.4804118643950588 1108 6 0.4390172052829584 1108 7 0.5571866669477075 1108 8 0.49931178874003024 1108 9 0.6191566344897552 1108 10-11 0.597997282008393 1108 12-13 0.6419763418924944 1108 14-15 0.5677462068790646 1108 16-17 0.7535824051707962 1108 18-19 0.9096321910744152 1108 20-21 0.9156929058130174 1108 22-23 0.8422251356895245 1108 24-25 0.9491254058247307 1108 26-27 1.0197635043167779 1108 28-29 1.120345917570667 1108 30-31 1.0329853049252549 1108 32-33 0.9859770211187637 1108 34-35 1.2027269049215121 1108 36-37 1.3250868378947231 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2311 42-43 0.1771907253426761 2311 44-45 0.22919469398139114 2311 46-47 0.22388250364233642 2311 48-49 0.2668437592182542 2311 50-51 0.4123828320884684 2311 52-53 0.3866660519892733 2311 54-55 0.42903478138156714 2311 56-57 0.6448845217811439 2311 58-59 0.4202201177859983 2311 60-61 0.3753936906077584 2311 62-63 0.6138232092353562 2311 64-65 0.7950618109065459 2311 66-67 0.6739780976545013 2311 68-69 0.48143973068380674 2311 70-71 0.5434187936473798 2311 72-73 0.6846143801827793 2311 74-75 0.8438560071116363 2311 76-77 1.0427428921650765 2311 78-79 1.24810849181209 2311 80-81 1.2256248479998035 2311 82-83 1.3917253093427533 2311 84-85 1.6036157251608039 2311 86-87 2.0457820791639243 2311 88-89 2.2379650005909753 2311 90-91 2.3105519044519447 2311 92-93 2.544087206259551 2311 94-95 2.4583474609177323 2311 96-97 2.335627557602187 2311 98-99 2.5456610373897774 2311 100-101 2.767563684491444 2312 1 -0.34345619758862256 2312 2 -0.26128830016494575 2312 3 -0.2271491300227062 2312 4 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0.7029491709769431 2312 64-65 0.6814597342674169 2312 66-67 0.7967047323372398 2312 68-69 0.8846587506524521 2312 70-71 0.9908098263799481 2312 72-73 0.9772211307192435 2312 74-75 1.1119812572756302 2312 76-77 1.1330722469643355 2312 78-79 1.278238660478216 2312 80-81 1.639133599570279 2312 82-83 1.9646861600671173 2312 84-85 2.0359276018355104 2312 86-87 2.396263054191998 2312 88-89 2.257630149132801 2312 90-91 2.589804240705707 2312 92-93 2.8282603030674593 2312 94-95 2.8937672777976893 2312 96-97 3.2387869898966883 2312 98-99 3.3305724654370543 2312 100-101 3.121482329770016 2313 1 -0.4096180831360421 2313 2 -0.2851858791397177 2313 3 -0.31266987964636783 2313 4 -0.18283090766568932 2313 5 -0.18903854992160518 2313 6 -0.3187011678081788 2313 7 -0.36810758454682 2313 8 -0.16781863773115901 2313 9 -0.4943283973980428 2313 10-11 -0.23620436755468432 2313 12-13 -0.18463978553600668 2313 14-15 -0.1773814619985714 2313 16-17 -0.200504839211483 2313 18-19 -0.08422741988092497 2313 20-21 -0.05044918087071437 2313 22-23 0.03181343681645643 2313 24-25 -0.13920764100228666 2313 26-27 -0.05492904825474909 2313 28-29 -0.014296119539146446 2313 30-31 -0.053530982780344516 2313 32-33 0.07319715140899063 2313 34-35 0.04200970234538204 2313 36-37 0.09140040039031305 2313 38-39 -0.04027777587958781 2313 40-41 0.11511448704602145 2313 42-43 0.2682500031747992 2313 44-45 0.43757770800911544 2313 46-47 0.4452189670508915 2313 48-49 0.5004173155735643 2313 50-51 0.7792688538234316 2313 52-53 0.8234782181358966 2313 54-55 0.8201418870081696 2313 56-57 0.8357511805059659 2313 58-59 1.0501994843169307 2313 60-61 1.175219347161768 2313 62-63 1.410601213681609 2313 64-65 1.4269912293834892 2313 66-67 1.4708174333819315 2313 68-69 1.6810700462235282 2313 70-71 1.6374534339791822 2313 72-73 1.7361029628433826 2313 74-75 1.8187886575880228 2313 76-77 1.843557272600716 2313 78-79 1.8291060813336664 2313 80-81 1.8712908827362895 2313 82-83 1.8000924868206347 2313 84-85 2.06644013140507 2313 86-87 2.100954250386721 2313 88-89 2.4575587365285294 2313 90-91 2.4985296300617996 2313 92-93 2.4983326565212955 2313 94-95 2.8549643072597206 2313 96-97 3.05023578504332 2313 98-99 3.162213802264027 2313 100-101 3.275590482196135 2314 1 -0.4829911809135794 2314 2 -0.4636102426931572 2314 3 -0.5526850727336594 2314 4 -0.4391114917425796 2314 5 -0.44691724052730564 2314 6 -0.33231031038644687 2314 7 -0.2642001577997277 2314 8 -0.3500906579033938 2314 9 -0.4434094044411836 2314 10-11 -0.327080381213932 2314 12-13 -0.3670661331962535 2314 14-15 -0.44360709096478956 2314 16-17 -0.3662280505014266 2314 18-19 -0.29014259277854393 2314 20-21 -0.3189425161019699 2314 22-23 -0.4715839466608358 2314 24-25 -0.4991205857741079 2314 26-27 -0.3985324825315715 2314 28-29 -0.31058419848509544 2314 30-31 -0.4479137890278082 2314 32-33 -0.18636443506306222 2314 34-35 -0.2914202823598586 2314 36-37 -0.20282513706878547 2314 38-39 -0.27813746786371496 2314 40-41 -0.4515602530664893 2314 42-43 -0.21933244401615326 2314 44-45 -0.03060450300631601 2314 46-47 0.13623227770595037 2314 48-49 0.20210445998487359 2314 50-51 0.3816010508222831 2314 52-53 0.5458663071040561 2314 54-55 0.5360041019743775 2314 56-57 0.3270952240459266 2314 58-59 0.5901737533856384 2314 60-61 0.549403408654559 2314 62-63 0.642027446594831 2314 64-65 0.7187237424450394 2314 66-67 0.8153199614477487 2314 68-69 1.1498269001796118 2314 70-71 1.0739173518493637 2314 72-73 1.2571005793007224 2314 74-75 1.1921121163829476 2314 76-77 1.4682491623099452 2314 78-79 1.480574879583699 2314 80-81 1.6151453806018452 2314 82-83 1.632568001402948 2314 84-85 1.971074821900384 2314 86-87 2.304987898862837 2314 88-89 2.4267326378257508 2314 90-91 2.4970157681852676 2314 92-93 2.5438394752544013 2314 94-95 2.6939651511587392 2314 96-97 2.8971456403603923 2314 98-99 2.965116357346229 2314 100-101 2.60265509348695 2315 1 -0.658535975102442 2315 2 -0.5241429303444889 2315 3 -0.46672865626876714 2315 4 -0.41247710917599534 2315 5 -0.2574499281786373 2315 6 -0.3662086154711943 2315 7 -0.4760645645793886 2315 8 -0.44512697751598296 2315 9 -0.4888089201796788 2315 10-11 -0.34433219719456787 2315 12-13 -0.5619813874335406 2315 14-15 -0.6448782774054678 2315 16-17 -0.34080432168786956 2315 18-19 -0.31284235064779153 2315 20-21 0.038805667917401365 2315 22-23 -0.31480428564389484 2315 24-25 -0.2506339029653901 2315 26-27 -0.22207969802794736 2315 28-29 -0.3529570798410333 2315 30-31 -0.1836886074297439 2315 32-33 -0.2565823527386115 2315 34-35 -0.31926531867947006 2315 36-37 -0.3105733210881567 2315 38-39 -0.25755635406225963 2315 40-41 -0.14617284386552143 2315 42-43 -0.0925164633866089 2315 44-45 -0.24700886135982358 2315 46-47 -0.04385246805676246 2315 48-49 -0.004614668344416373 2315 50-51 -0.0027815157636723598 2315 52-53 0.20627792938008582 2315 54-55 0.3153624554852783 2315 56-57 0.5592380811887878 2315 58-59 0.631335980988549 2315 60-61 0.6759167258458412 2315 62-63 0.6274996015585117 2315 64-65 0.6527431129050889 2315 66-67 0.9218574917140927 2315 68-69 0.8311222996953518 2315 70-71 0.9247042767888303 2315 72-73 1.3724153492765083 2315 74-75 1.4215310025814958 2315 76-77 1.439193472237303 2315 78-79 1.642197155612756 2315 80-81 2.1487410222483376 2315 82-83 2.2687665486184443 2315 84-85 2.39722494296576 2315 86-87 2.5014164702914066 2315 88-89 2.6700740421841047 2315 90-91 2.763056930412869 2315 92-93 2.819565867506217 2315 94-95 2.808069267381498 2315 96-97 2.6737800229269766 2315 98-99 2.921230158799016 2315 100-101 2.927412962736346 2316 1 -0.8308974983810984 2316 2 -0.8577879935160766 2316 3 -0.7223363211190659 2316 4 -0.5974389994316596 2316 5 -0.530127502303209 2316 6 -0.4402760880057883 2316 7 -0.5815685252458707 2316 8 -0.5956967332401675 2316 9 -0.7408301486730267 2316 10-11 -0.6337808751977008 2316 12-13 -0.49131477940913015 2316 14-15 -1.043235059921436 2316 16-17 -0.8398685534667223 2316 18-19 -0.5987098006514273 2316 20-21 -0.4336018046480632 2316 22-23 -0.6305414726987664 2316 24-25 -0.6297309512541958 2316 26-27 -0.5063640002700822 2316 28-29 -0.6236377311403416 2316 30-31 -0.4920692789452019 2316 32-33 -0.20179679817585594 2316 34-35 -0.46504280684685284 2316 36-37 -0.22501929846150404 2316 38-39 -0.4452717292877679 2316 40-41 -0.3276887305131453 2316 42-43 -0.24388714874472583 2316 44-45 -0.20843152226720463 2316 46-47 -0.10458808874636816 2316 48-49 0.02401374351551766 2316 50-51 0.08926134863229862 2316 52-53 0.11335465967359681 2316 54-55 0.09661221435761291 2316 56-57 0.37701462953700116 2316 58-59 0.7121359306796684 2316 60-61 0.47406799043503156 2316 62-63 0.7955802408207333 2316 64-65 0.8835591892140826 2316 66-67 1.080274891794648 2316 68-69 1.176475171699387 2316 70-71 0.8781699178334641 2316 72-73 1.142176050329141 2316 74-75 1.493489090350419 2316 76-77 1.5117798422359279 2316 78-79 1.4663354232281947 2316 80-81 1.8669810372484044 2316 82-83 2.1364194852832767 2316 84-85 2.1945785572571843 2316 86-87 2.3550324978347454 2316 88-89 2.2982376370772926 2316 90-91 2.6718209756167823 2316 92-93 2.8513333756891264 2316 94-95 2.9614814359563546 2316 96-97 2.567032808047898 2316 98-99 2.604521516629376 2316 100-101 2.494772649586748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4412.0 3 1462.0 4 1578.0 5 1783.0 6 2222.0 7 2921.0 8 3676.0 9 4865.0 10 6165.0 11 8113.0 12 10565.0 13 13160.0 14 15238.0 15 19344.0 16 22949.0 17 27277.0 18 24934.0 19 21463.0 20 22524.0 21 24009.0 22 26787.0 23 29658.0 24 33599.0 25 37552.0 26 42158.0 27 47635.0 28 53162.0 29 60036.0 30 67469.0 31 76752.0 32 87987.0 33 102193.0 34 120444.0 35 142650.0 36 160900.0 37 160956.0 38 84209.0 39 5748.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.73500949911998 18.27141432047934 14.60233951570373 22.39123666469695 2 15.798759308513166 20.368113633094232 39.64674069750715 24.186386360885457 3 19.186042458477274 25.04729474082482 29.54753588854856 26.219126912149342 4 12.896397344210678 16.708200345283032 36.00076307043318 34.39463924007311 5 13.956827236049351 37.458568788963085 32.82258345667352 15.762020518314038 6 31.947876112589423 35.477483256487915 17.288571562588345 15.28606906833432 7 29.1604803555711 33.313245644551834 19.57225262835051 17.954021371526558 8 27.483324102391215 33.497800416469694 19.368882388503106 19.649993092635988 9 27.863942491417504 16.449682842170173 19.510609213008763 36.175765453403564 10-11 25.600244860170207 26.59533783447668 26.15615475745757 21.64826254789554 12-13 26.87325057556341 24.18104598233608 26.937191504031322 22.008511938069187 14-15 23.509960002838834 25.027152698921242 26.07543064117122 25.387456657068704 16-17 22.27207146355697 28.605445560552095 26.498492272540354 22.623990703350582 18-19 22.317190398008783 26.207488478459645 30.021652194906505 21.453668928625063 20-21 23.126937672536826 25.2458293301832 30.11098760143074 21.51624539584923 22-23 22.73265774479153 25.26945754885121 30.059159148597477 21.938725557759778 24-25 22.48194678699395 25.633534195729595 30.105178624285905 21.77934039299054 26-27 21.924518543963877 26.04774441665642 29.991862179288102 22.035874860091596 28-29 21.8289363155675 26.17798027750659 30.25626059725168 21.73682280967423 30-31 21.754610107063233 25.81292388966416 30.16389660989666 22.268569393375945 32-33 21.607515723768635 26.04584844980276 29.939006036026043 22.40762979040256 34-35 22.585749487576848 26.039112406105925 29.54272748847226 21.832410617844968 36-37 22.46867438309597 25.65806212242045 29.612977694468817 22.260285800014763 38-39 21.93938633599484 26.03597104752346 29.717029998260724 22.307612618220976 40-41 22.275407176959217 25.826820297228885 29.73613095558674 22.161641570225154 42-43 22.245035997433845 25.90237527621793 29.35663992269858 22.49594880364964 44-45 22.055648335162175 26.220720572322055 29.23162647014848 22.49200462236729 46-47 22.238033975346124 26.564294365141038 28.66013660974541 22.537535049767424 48-49 22.465102310799782 26.50101879964114 28.440286176257406 22.59359271330167 50-51 22.546055363683504 26.795436165842073 28.064494772890047 22.594013697584376 52-53 22.815353918794486 26.79349359968299 27.9292723078234 22.461880173699132 54-55 22.18155255631288 26.85196829392142 28.169285346154467 22.797193803611236 56-57 22.29200540653377 27.10067816077754 28.115559432751088 22.4917569999376 58-59 22.413112045445256 26.384535485180972 28.20448085482599 22.99787161454778 60-61 22.23152111558 26.680049048318804 28.521388317035107 22.56704151906609 62-63 22.142687450308365 26.831930617015683 28.374739069424166 22.65064286325179 64-65 21.97007757782332 26.63206444439517 28.297659360814485 23.10019861696702 66-67 22.03999393100285 26.91330995058962 28.01390167781164 23.03279444059589 68-69 22.165981386567665 26.558257255627428 28.301508460939033 22.974252896865874 70-71 22.068248480757102 26.902803618115414 27.949127419360458 23.079820481767026 72-73 22.3617901013987 26.730855008223912 27.912995050445915 22.994359839931473 74-75 22.100144959489903 27.122408405623013 27.74437838481676 23.033068250070325 76-77 22.24673727212223 26.81298825294421 27.896937111135962 23.043337363797605 78-79 22.445671661837057 26.909870265791042 27.696999223292607 22.94745884907929 80-81 22.333523393202192 27.230891064018497 27.5501916437011 22.88539389907821 82-83 22.434386821008257 27.028489372358216 27.460678343128837 23.076445463504694 84-85 22.415853677464888 26.748614415526784 27.49141503041998 23.34411687658835 86-87 22.670851129336135 26.71604991454118 27.39291040190353 23.220188554219156 88-89 22.569248198621754 26.809995463289393 27.334124934420455 23.2866314036684 90-91 22.47742596963219 26.75013489446548 27.471135119104762 23.30130401679757 92-93 22.700695635260175 26.373063117202456 27.511124810441846 23.41511643709552 94-95 22.560255430560677 26.24943131343469 27.59774122131697 23.592572034687663 96-97 22.961584276354973 26.06468515960615 27.367227199574213 23.606503364464665 98-99 22.746079963455127 26.16975436433428 27.033006649101726 24.051159023108866 100-101 23.240592415588896 25.645584342613763 27.432927084482767 23.68089615731457 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1427.0 1 950.5 2 643.0 3 831.5 4 706.5 5 667.0 6 1241.0 7 2671.0 8 4074.5 9 4416.0 10 3678.5 11 2916.0 12 2803.0 13 2932.5 14 3095.0 15 3184.5 16 3256.0 17 3393.5 18 3575.0 19 3847.0 20 4131.5 21 4437.0 22 5028.0 23 5720.5 24 6584.5 25 7773.0 26 9143.5 27 10786.5 28 12249.0 29 13867.0 30 15817.5 31 18325.5 32 21498.5 33 23519.5 34 25345.0 35 27375.0 36 29455.5 37 32074.0 38 34236.5 39 35514.5 40 37307.5 41 41353.0 42 45808.5 43 49795.5 44 52943.5 45 55399.5 46 56301.0 47 57611.5 48 61534.0 49 62882.5 50 61891.0 51 60591.5 52 58648.0 53 57963.5 54 57572.5 55 53924.5 56 48082.5 57 43045.0 58 39347.5 59 35282.0 60 30011.0 61 24891.5 62 20518.0 63 16155.5 64 12499.0 65 10063.5 66 7765.5 67 6094.5 68 4667.0 69 3370.5 70 2630.0 71 2166.5 72 1911.0 73 1624.5 74 1299.5 75 1053.5 76 775.5 77 497.5 78 302.5 79 194.0 80 119.5 81 72.0 82 36.0 83 19.5 84 12.5 85 8.5 86 8.5 87 8.0 88 3.0 89 2.5 90 5.0 91 3.5 92 1.0 93 0.0 94 0.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29932438210895407 2 0.035728878626338646 3 0.0433307676957724 4 0.04554798534102392 5 0.05954813104389774 6 0.058851291212532984 7 0.03002746182426333 8 0.033511660981087134 9 0.04016331391684167 10-11 0.03240305215846138 12-13 0.03395510451013743 14-15 0.028063640481326278 16-17 0.022298874603672345 18-19 0.0268600080453326 20-21 0.03997326669010582 22-23 0.033163241065404754 24-25 0.05435350684645134 26-27 0.046688268701438974 28-29 0.03867461064074423 30-31 0.0385795870273763 32-33 0.03357501005666575 34-35 0.032529750309618605 36-37 0.03126276879804631 38-39 0.019859935193895683 40-41 0.019638213429370534 42-43 0.02011333149621014 44-45 0.00905891780774189 46-47 0.01669248141496495 48-49 0.014380240156345519 50-51 0.006429931171229384 52-53 0.004972902432921248 54-55 0.007570214531644447 56-57 0.005986487642179081 58-59 0.008140356211851978 60-61 0.00706342192701553 62-63 0.004434435290503023 64-65 0.008868870581006046 66-67 0.002565637560933892 68-69 0.007918634447326827 70-71 0.013905122089505909 72-73 0.014633636458659977 74-75 0.011592880830886476 76-77 0.010104177554789033 78-79 0.006144860331125618 80-81 0.006651652935754535 82-83 0.012321395200040542 84-85 0.004402760752713716 86-87 0.0010769342848364486 88-89 0.020208355109578066 90-91 0.029742390984159563 92-93 0.013525027636034222 94-95 0.02261561998156542 96-97 0.01868797729569131 98-99 0.014031820240663139 100-101 0.1069965886522801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1578555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.07914232579712 #Duplication Level Percentage of deduplicated Percentage of total 1 75.14013609032486 36.12673297466494 2 12.992654559869843 12.49351375547798 3 4.6710631758241785 6.737421337297217 4 2.220942819450805 4.271241036553295 5 1.2643619262428192 3.039471850157375 6 0.7673701329093082 2.213669870202749 7 0.5243245503758776 1.7646352277701975 8 0.3703235439262784 1.4243870700020096 9 0.26126752583225343 1.130536670363801 >10 1.5474654318750152 13.856087252010282 >50 0.14318086343929662 4.7652365801258565 >100 0.0901390925008636 7.9960549616175465 >500 0.005044527888007887 1.6523761504991974 >1k 0.001327507338949444 0.9074697403452273 >5k 2.6550146778988877E-4 0.916405298345532 >10k+ 1.3275073389494438E-4 0.704760224566887 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11042 0.6995004925390627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8281 0.5245936948665076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6077 0.38497233229124106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2051 0.1299289540117386 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1703 0.1078834757103807 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1617 0.10243545521061984 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.334907557861462E-5 0.0 12-13 0.0 0.0 0.0 6.334907557861462E-5 0.0 14-15 0.0 0.0 0.0 9.502361336792193E-5 0.0 16-17 0.0 0.0 0.0 1.2669815115722924E-4 0.0 18-19 0.0 0.0 0.0 1.2669815115722924E-4 0.0 20-21 0.0 0.0 0.0 1.2669815115722924E-4 0.0 22-23 0.0 0.0 0.0 5.384671424182243E-4 0.0 24-25 0.0 0.0 0.0 0.0011402833604150632 0.0 26-27 0.0 0.0 0.0 0.002280566720830126 0.0 28-29 0.0 0.0 0.0 0.006524954784597306 0.0 30-31 0.0 0.0 0.0 0.020746822251996287 0.0 32-33 0.0 0.0 0.0 0.044629423745134 0.0 34-35 0.0 0.0 0.0 0.07893294817095381 0.0 36-37 0.0 0.0 0.0 0.13756251761896165 0.0 38-39 0.0 0.0 0.0 0.2258711289755504 0.0 40-41 0.0 0.0 0.0 0.322921912761988 0.0 42-43 0.0 0.0 0.0 0.4194025548682181 0.0 44-45 0.0 0.0 0.0 0.5400508693076896 0.0 46-47 0.0 0.0 0.0 0.6830930819642014 0.0 48-49 0.0 0.0 0.0 0.8378548736027569 0.0 50-51 0.0 0.0 0.0 1.0128566948886797 0.0 52-53 0.0 0.0 0.0 1.180129928954012 0.0 54-55 0.0 0.0 0.0 1.363842248131994 0.0 56-57 0.0 0.0 0.0 1.5723874049367934 0.0 58-59 0.0 0.0 0.0 1.8036432053365261 0.0 60-61 0.0 0.0 0.0 2.051623161689013 0.0 62-63 0.0 0.0 0.0 2.307300030724302 0.0 64-65 0.0 0.0 0.0 2.561298149256757 0.0 66-67 0.0 0.0 0.0 2.860020715147714 0.0 68-69 0.0 0.0 0.0 3.1841779348834853 0.0 70-71 0.0 0.0 0.0 3.531901010734501 0.0 72-73 0.0 0.0 0.0 3.8804476245680384 0.0 74-75 0.0 0.0 0.0 4.240618793770252 0.0 76-77 0.0 0.0 0.0 4.6254010788347575 0.0 78-79 0.0 0.0 0.0 5.042206321604253 0.0 80-81 0.0 0.0 0.0 5.499269901903956 0.0 82-83 0.0 0.0 0.0 5.966501008834029 0.0 84-85 0.0 0.0 0.0 6.428537491566654 0.0 86-87 0.0 0.0 0.0 6.901026571769751 3.167453778930731E-5 88-89 0.0 0.0 0.0 7.404905118922052 6.334907557861462E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4890 0.0 54.653946 1 GTATCAA 8955 0.0 42.02706 1 TATCAAC 11745 0.0 31.804546 2 ATCAACG 11885 0.0 31.389915 3 TCAACGC 12055 0.0 30.947254 4 CAACGCA 12270 0.0 30.404984 5 TATTACG 40 4.5025622E-4 29.679432 70-71 AACGCAG 12675 0.0 29.582504 6 GTACATG 9710 0.0 25.855854 1 ACGCAGA 14535 0.0 25.530499 7 CGCAGAG 14635 0.0 25.323584 8 TATAACG 95 0.0012230403 25.013018 2 TACATGG 9995 0.0 24.82032 2 GCAGAGT 15010 0.0 24.214556 9 TGGTATC 2130 0.0 23.873926 2 ACCGAGT 545 0.0 23.539139 8 ACATGGG 10225 0.0 23.425394 3 GAGTACT 8160 0.0 23.315948 12-13 GGACCGA 505 0.0 22.585297 6 TAAGGTG 595 0.0 22.36458 5 >>END_MODULE