FastQCFastQC Report
Thu 26 May 2016
SRR938649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578555
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105160.6661788787847113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84940.5380870479647526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63880.4046738947961902No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20570.13030904846521027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19890.12600131132586448No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG18490.11713244074485843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15890.10066168109441863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46750.057.4883881
GTATCAA89050.044.4429321
TATCAAC120150.032.7412342
ATCAACG121750.032.218513
TCAACGC124300.031.5958024
CAACGCA126550.031.0716155
AACGCAG129100.030.387336
ACGCAGA146600.026.4683487
CGCAGAG146850.026.3586038
GTACATG93250.025.709581
GCAGAGT151850.025.0840879
TACATGG94850.024.5737842
TGGTATC19450.024.4564672
GAGTACT87700.024.12608512-13
GTCTACG1000.001640238423.7839131
GTGGTAT21400.023.7839131
GTACGTA1201.694734E-423.7839131
ACAATCG1000.001655135523.7469988
AGTACTT88950.023.68025612-13
GTACTTT94200.022.61258714-15
Produced by FastQC (version 0.11.5)