##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938649_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1578555 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.739962814092635 31.0 31.0 34.0 30.0 34.0 2 31.971108387100863 31.0 31.0 34.0 30.0 34.0 3 32.017259455641394 33.0 31.0 34.0 30.0 34.0 4 35.7166991330679 37.0 35.0 37.0 33.0 37.0 5 35.59181783339827 37.0 35.0 37.0 33.0 37.0 6 35.64269600995848 37.0 35.0 37.0 33.0 37.0 7 35.572512202615684 37.0 35.0 37.0 33.0 37.0 8 35.56246313875665 37.0 35.0 37.0 33.0 37.0 9 37.099019039564666 39.0 37.0 39.0 33.0 39.0 10-11 37.03434438457957 39.0 37.0 39.0 33.0 39.0 12-13 36.91988400784261 39.0 37.0 39.0 33.0 39.0 14-15 37.90307844832775 40.0 37.0 41.0 32.5 41.0 16-17 37.84027639201675 40.0 37.0 41.0 32.5 41.0 18-19 37.880672197040965 40.0 37.0 41.0 33.0 41.0 20-21 37.89452632312462 40.0 37.0 41.0 32.5 41.0 22-23 37.914628251787235 40.0 37.0 41.0 32.5 41.0 24-25 37.82131126251541 40.0 37.0 41.0 32.0 41.0 26-27 37.633847094336275 40.0 37.0 41.0 32.0 41.0 28-29 37.487975711964424 39.5 36.5 41.0 32.0 41.0 30-31 37.19432455631891 39.0 36.0 41.0 31.0 41.0 32-33 36.92512424337448 39.0 35.0 40.0 31.0 41.0 34-35 36.70817614843955 39.0 35.0 40.0 30.0 41.0 36-37 36.66424831570645 39.0 35.0 40.0 30.0 41.0 38-39 36.49900478602266 39.0 35.0 40.0 30.0 41.0 40-41 36.28633623788845 39.0 35.0 40.0 30.0 41.0 42-43 35.9999699091891 38.0 35.0 40.0 29.5 41.0 44-45 35.6211193148164 38.0 35.0 40.0 28.5 41.0 46-47 35.125765019273956 38.0 34.0 40.0 26.5 41.0 48-49 34.624650709034526 38.0 33.5 40.0 24.5 41.0 50-51 34.20664563477357 38.0 33.0 40.0 23.5 41.0 52-53 33.67344723497122 37.0 33.0 40.0 22.5 41.0 54-55 33.328555546053195 37.0 32.5 40.0 21.5 41.0 56-57 32.952167646993615 36.5 32.0 39.5 19.5 41.0 58-59 32.48694217179636 36.0 31.0 39.0 18.0 40.5 60-61 32.06884429113968 35.5 31.0 39.0 14.5 40.0 62-63 31.587542721032843 35.0 30.0 39.0 12.0 40.0 64-65 31.08462707982934 35.0 29.5 38.0 8.0 40.0 66-67 30.580132146171657 34.5 29.0 38.0 7.5 40.0 68-69 30.00833800532766 34.0 28.5 37.0 4.0 39.0 70-71 29.432525632619708 34.0 28.0 36.5 2.0 39.0 72-73 28.849348929875738 33.5 26.5 36.0 2.0 39.0 74-75 28.231634627871692 33.0 26.0 35.5 2.0 38.0 76-77 26.982895116103016 31.5 25.5 34.0 2.0 36.0 78-79 27.611429123470515 32.5 26.0 35.0 2.0 37.0 80-81 27.30957077833842 33.0 26.0 35.0 2.0 36.0 82-83 26.819246716142295 32.5 25.0 35.0 2.0 36.0 84-85 26.236146982525156 32.0 24.0 35.0 2.0 35.5 86-87 25.584099698775148 31.5 21.0 34.0 2.0 35.0 88-89 24.839612493704685 31.0 19.0 34.0 2.0 35.0 90-91 24.2943175879206 31.0 16.0 34.0 2.0 35.0 92-93 23.512062297480924 30.5 4.5 34.0 2.0 35.0 94-95 22.708068455011073 30.0 2.0 34.0 2.0 35.0 96-97 21.724995328005676 29.5 2.0 34.0 2.0 35.0 98-99 20.56482162484044 29.0 2.0 34.0 2.0 35.0 100-101 17.547699636693054 22.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41814274303121834 1101 2 0.39045592062833734 1101 3 0.33333809158293803 1101 4 0.2003931974448605 1101 5 0.1393896032719013 1101 6 0.316852001641017 1101 7 0.2753851138961778 1101 8 0.34090291315066423 1101 9 0.33862159163957983 1101 10-11 0.3557559187070183 1101 12-13 0.4044953048165212 1101 14-15 0.5468292900301677 1101 16-17 0.575276538152643 1101 18-19 0.47532325238040585 1101 20-21 0.5495653538079353 1101 22-23 0.48759680561186514 1101 24-25 0.5499770070522132 1101 26-27 0.5134754036275524 1101 28-29 0.4820451881884935 1101 30-31 0.5764256094929223 1101 32-33 0.6308544272748264 1101 34-35 0.44012267384848514 1101 36-37 0.6462623860575221 1101 38-39 0.8028188341063824 1101 40-41 0.9540245082323082 1101 42-43 0.8895158597640815 1101 44-45 0.9517710853580894 1101 46-47 1.034594389833309 1101 48-49 1.2457588666064723 1101 50-51 1.132766478378727 1101 52-53 1.3355306083454792 1101 54-55 1.2560402151669408 1101 56-57 1.384156151405925 1101 58-59 1.4147971001797472 1101 60-61 1.393474668742293 1101 62-63 1.613046872927871 1101 64-65 1.5395360025955895 1101 66-67 1.600183426377992 1101 68-69 1.6299594191683546 1101 70-71 1.9138505142425828 1101 72-73 2.098957142831882 1101 74-75 2.0039910659316043 1101 76-77 1.9198754742273358 1101 78-79 2.235046147903656 1101 80-81 2.517636058865829 1101 82-83 2.6941039859606057 1101 84-85 2.924057927165215 1101 86-87 2.9597247176929535 1101 88-89 3.063051509295164 1101 90-91 2.9890232997017705 1101 92-93 3.2843494964917497 1101 94-95 3.3672564335542 1101 96-97 3.87584277312353 1101 98-99 4.201751192266311 1101 100-101 4.491508598597417 1102 1 0.4132320842176327 1102 2 0.3744714279719581 1102 3 0.40122248234811053 1102 4 0.31831362290274967 1102 5 0.2491537624209883 1102 6 0.34466084380905926 1102 7 0.36436098307781606 1102 8 0.3381617913534072 1102 9 0.4569346709904636 1102 10-11 0.502612202411882 1102 12-13 0.5356752546164714 1102 14-15 0.6178126643684934 1102 16-17 0.4934380232790119 1102 18-19 0.6153118206847878 1102 20-21 0.6651658906006048 1102 22-23 0.6325805278713688 1102 24-25 0.5404775165082114 1102 26-27 0.6159406736392299 1102 28-29 0.6390508294817607 1102 30-31 0.579103359934031 1102 32-33 0.37709315090116036 1102 34-35 0.6539115354007095 1102 36-37 0.7126643841191012 1102 38-39 0.9116224823083741 1102 40-41 0.8650175746603992 1102 42-43 0.7872817088159962 1102 44-45 0.7168323443356996 1102 46-47 0.8415490855265446 1102 48-49 0.903351003588007 1102 50-51 0.9355908527419103 1102 52-53 1.125116582151989 1102 54-55 1.0758667516116915 1102 56-57 1.3101197687920418 1102 58-59 1.3228502327128595 1102 60-61 1.245039071436203 1102 62-63 1.4365004875803216 1102 64-65 1.2321178261995556 1102 66-67 1.187563828475458 1102 68-69 1.4651280366787311 1102 70-71 1.5931405065386102 1102 72-73 1.6497101436519834 1102 74-75 1.6807300504988127 1102 76-77 1.6648964737551566 1102 78-79 2.000688932267085 1102 80-81 2.356732457881712 1102 82-83 2.1473843855729235 1102 84-85 2.387430525887847 1102 86-87 2.314309821696529 1102 88-89 2.725105735294818 1102 90-91 2.9452336926041838 1102 92-93 2.959903063926326 1102 94-95 3.081739894872822 1102 96-97 3.567123620063068 1102 98-99 4.051689063485778 1102 100-101 4.5154082978941155 1103 1 0.13279995343311057 1103 2 0.25065617094123027 1103 3 0.32713589439200064 1103 4 0.17647441007175502 1103 5 0.19240031110113165 1103 6 0.23466593572528893 1103 7 0.25253710944617325 1103 8 0.2874750171695766 1103 9 0.38794713746074905 1103 10-11 0.4314268963179231 1103 12-13 0.4123453952072893 1103 14-15 0.5754692566551185 1103 16-17 0.5007875936386696 1103 18-19 0.5277498967970402 1103 20-21 0.5327526712881223 1103 22-23 0.5099859030948366 1103 24-25 0.4014927906706234 1103 26-27 0.35586867299203817 1103 28-29 0.2666370391132631 1103 30-31 0.4239085743100546 1103 32-33 0.42755864087516215 1103 34-35 0.5940442424759382 1103 36-37 0.6620529576052903 1103 38-39 0.6654735413793631 1103 40-41 0.660887253330344 1103 42-43 0.7963507923186626 1103 44-45 0.9115402412474012 1103 46-47 1.0665023303144707 1103 48-49 0.9029901279053192 1103 50-51 1.013193400920791 1103 52-53 1.1908149916023376 1103 54-55 1.1752447764240728 1103 56-57 1.2116697100208569 1103 58-59 1.3389175284929138 1103 60-61 1.3666216580344681 1103 62-63 1.4161062527081505 1103 64-65 1.295752106058245 1103 66-67 1.1884743455825522 1103 68-69 1.4711011240772685 1103 70-71 1.514608406051785 1103 72-73 1.439486971784742 1103 74-75 1.5698650751097425 1103 76-77 1.4656062488066688 1103 78-79 1.9028670351237942 1103 80-81 2.236638979182896 1103 82-83 2.3835699851262326 1103 84-85 2.4295300450903987 1103 86-87 2.502465643850652 1103 88-89 2.8325689616661833 1103 90-91 3.03641587375267 1103 92-93 3.106126718018654 1103 94-95 3.05278695782064 1103 96-97 3.5613246254389246 1103 98-99 4.084152805393831 1103 100-101 4.2475647799352 1104 1 0.2585624196738081 1104 2 0.25887697325991255 1104 3 0.27953692199228897 1104 4 0.1881468954152794 1104 5 0.12043535490588653 1104 6 0.13749183652233654 1104 7 0.15966443966156874 1104 8 0.11630710991709492 1104 9 0.3121682032666868 1104 10-11 0.29122203497809096 1104 12-13 0.2404430172348242 1104 14-15 0.4108127781622599 1104 16-17 0.49963154331259574 1104 18-19 0.5423339162950995 1104 20-21 0.505705704685127 1104 22-23 0.40894183485163893 1104 24-25 0.42398801496414507 1104 26-27 0.5376584101634947 1104 28-29 0.49642427315581017 1104 30-31 0.42486042201441165 1104 32-33 0.46764493295078324 1104 34-35 0.36432288024683146 1104 36-37 0.4234553685136646 1104 38-39 0.5432385107489708 1104 40-41 0.5678531976027941 1104 42-43 0.5206476107100784 1104 44-45 0.6808044530911488 1104 46-47 0.6851275855677486 1104 48-49 0.9005232284915792 1104 50-51 0.8987279506869541 1104 52-53 1.1123451938288014 1104 54-55 1.0971306864431725 1104 56-57 1.0817309708073637 1104 58-59 1.275357815694015 1104 60-61 1.2645960997708485 1104 62-63 1.2414438457520909 1104 64-65 1.2082150531631868 1104 66-67 1.312481327994778 1104 68-69 1.4871145619272106 1104 70-71 1.3904071017898794 1104 72-73 1.6599994544933914 1104 74-75 1.5846928203175672 1104 76-77 1.615988305324688 1104 78-79 1.9255862235830499 1104 80-81 2.221557627493283 1104 82-83 2.4327004771886784 1104 84-85 2.632915856301782 1104 86-87 2.6264429839468235 1104 88-89 2.8527487917169054 1104 90-91 3.022408232622311 1104 92-93 3.3538541404545974 1104 94-95 3.5472701934441204 1104 96-97 3.9314155285414856 1104 98-99 4.2727866239828565 1104 100-101 4.478333503998172 1105 1 0.2816895999214495 1105 2 0.23699497500694733 1105 3 0.1760977670558077 1105 4 0.19151530137479966 1105 5 0.12387658514570177 1105 6 0.17325233142558005 1105 7 0.26692781263319887 1105 8 0.25791071566228396 1105 9 0.2403517657323775 1105 10-11 0.3052475008365221 1105 12-13 0.39758536228984553 1105 14-15 0.5099400187823662 1105 16-17 0.5115492556491503 1105 18-19 0.39760282843995753 1105 20-21 0.37329908861813266 1105 22-23 0.4589945664224686 1105 24-25 0.2508020658996344 1105 26-27 0.3468672856698234 1105 28-29 0.5234235920789985 1105 30-31 0.6312642420818477 1105 32-33 0.752156000920742 1105 34-35 0.5688085540506549 1105 36-37 0.5525103697116762 1105 38-39 0.5854250880643974 1105 40-41 0.44632117095589763 1105 42-43 0.518894168064854 1105 44-45 0.49556551964612794 1105 46-47 0.5974888468239072 1105 48-49 0.6063878470225248 1105 50-51 0.7357602292002028 1105 52-53 0.6684170596962815 1105 54-55 0.6567254740358734 1105 56-57 0.7477991954608356 1105 58-59 0.9566576347652997 1105 60-61 1.0450757967010986 1105 62-63 1.0553710176985227 1105 64-65 1.2324474267630592 1105 66-67 1.418236144113468 1105 68-69 1.3385081281095772 1105 70-71 1.376164861066016 1105 72-73 1.399984134981576 1105 74-75 1.3835264998492285 1105 76-77 1.409660516824509 1105 78-79 1.8042805936624582 1105 80-81 2.044581608566517 1105 82-83 2.1052377996323273 1105 84-85 2.419676930403316 1105 86-87 2.6173384395985337 1105 88-89 2.797310270691252 1105 90-91 2.959193927367828 1105 92-93 3.1781212282191724 1105 94-95 3.085295410266152 1105 96-97 3.2333848027324485 1105 98-99 3.781922297881014 1105 100-101 4.167026874158704 1106 1 0.2148586806883337 1106 2 0.28147854506272907 1106 3 0.22416564311637188 1106 4 0.20905282539860792 1106 5 0.1228606942724042 1106 6 0.13268307253090939 1106 7 0.25055655683232914 1106 8 0.12259622637338907 1106 9 0.17729228042792045 1106 10-11 0.2596754762736424 1106 12-13 0.27208102628459585 1106 14-15 0.3794205419155432 1106 16-17 0.3568994642461547 1106 18-19 0.33419102513052934 1106 20-21 0.45188812907188947 1106 22-23 0.2700392389954729 1106 24-25 0.2923671528541192 1106 26-27 0.372880129620512 1106 28-29 0.3758812867304755 1106 30-31 0.4153476808855814 1106 32-33 0.45159349213154343 1106 34-35 0.38128406148046423 1106 36-37 0.6126575595115185 1106 38-39 0.45370671746485414 1106 40-41 0.5019279773122491 1106 42-43 0.6244399422175775 1106 44-45 0.06014715373415669 1106 46-47 -0.10453280976447132 1106 48-49 0.5191324211564989 1106 50-51 0.3244959906964766 1106 52-53 0.6093425741574521 1106 54-55 0.7000170879673462 1106 56-57 0.6858062268207021 1106 58-59 1.170934857846909 1106 60-61 0.9224108175607952 1106 62-63 1.147847275140041 1106 64-65 0.7874967869093155 1106 66-67 1.0946218376399308 1106 68-69 0.9745315659757949 1106 70-71 1.3099641391797334 1106 72-73 1.7131864037670255 1106 74-75 2.068618516911993 1106 76-77 1.9150690388326659 1106 78-79 2.199796273594959 1106 80-81 2.332417356000068 1106 82-83 2.5452291744975604 1106 84-85 2.5502590332486754 1106 86-87 2.745344627195216 1106 88-89 3.0574175223670608 1106 90-91 2.9521515048299776 1106 92-93 2.693173025903512 1106 94-95 2.9958617628654096 1106 96-97 3.3627839495941174 1106 98-99 3.706157426554899 1106 100-101 4.052403520668808 1107 1 0.24072850317354266 1107 2 0.18133653322840715 1107 3 0.22371593897435815 1107 4 0.2250054881205017 1107 5 0.009819274154061475 1107 6 0.0625765636551705 1107 7 0.0799293378974113 1107 8 0.2199453387994268 1107 9 0.1871384342740754 1107 10-11 0.35810151177659577 1107 12-13 0.32984433989406625 1107 14-15 0.4273258673593219 1107 16-17 0.3772663281514781 1107 18-19 0.4386407292725423 1107 20-21 0.4567993716754444 1107 22-23 0.3212936768653023 1107 24-25 0.3066038392446515 1107 26-27 0.2632588278453696 1107 28-29 0.2055262571446761 1107 30-31 0.23347785840037005 1107 32-33 0.33532130278242533 1107 34-35 0.44773376562246625 1107 36-37 0.4371546009316418 1107 38-39 0.3185824571098266 1107 40-41 0.3514546045311846 1107 42-43 0.5037653860045594 1107 44-45 0.6133424200063473 1107 46-47 0.6682719239633386 1107 48-49 0.8307300542925944 1107 50-51 0.7561527954302036 1107 52-53 0.7060763019681033 1107 54-55 0.7155792181448604 1107 56-57 0.6649659901343128 1107 58-59 0.7267455087344885 1107 60-61 0.9967658471465981 1107 62-63 1.1683916538382597 1107 64-65 1.1335086212288417 1107 66-67 1.4393082281724752 1107 68-69 1.4746262405320074 1107 70-71 1.4332185770495585 1107 72-73 1.589269244003713 1107 74-75 1.3442161500480871 1107 76-77 1.370749512205446 1107 78-79 1.5089797055476204 1107 80-81 1.841884811621373 1107 82-83 1.9481995886987455 1107 84-85 2.273667317272345 1107 86-87 2.54381799997628 1107 88-89 2.802245924733924 1107 90-91 2.830837895362521 1107 92-93 3.1085576412881295 1107 94-95 2.957601407835824 1107 96-97 3.3125117602450054 1107 98-99 3.6351870123537147 1107 100-101 3.9682615088344875 1108 1 0.29475477508145786 1108 2 0.2837132473402342 1108 3 0.2534044613730231 1108 4 0.23958168298572957 1108 5 0.21716893537731607 1108 6 0.225865547606368 1108 7 0.30537664281055754 1108 8 0.2545786648623718 1108 9 0.28543716289384946 1108 10-11 0.3632415606054735 1108 12-13 0.48173839388726947 1108 14-15 0.575686159769738 1108 16-17 0.5679754726610398 1108 18-19 0.5222056597230491 1108 20-21 0.517008407129147 1108 22-23 0.49155062653778003 1108 24-25 0.5018880528815615 1108 26-27 0.5871093439258246 1108 28-29 0.6178798232712239 1108 30-31 0.6589448637265178 1108 32-33 0.870522422891618 1108 34-35 0.7508953301166983 1108 36-37 0.7983001095892632 1108 38-39 0.9122769177758414 1108 40-41 1.0079180989622927 1108 42-43 1.0930251429858941 1108 44-45 1.0874296928359612 1108 46-47 1.1411754130471934 1108 48-49 1.1078701875518178 1108 50-51 1.1825861763728724 1108 52-53 1.2685336470560173 1108 54-55 1.3920924561188102 1108 56-57 1.3159576948532674 1108 58-59 1.5125724687496884 1108 60-61 1.2971215099599576 1108 62-63 1.5080415493557133 1108 64-65 1.8281793077178747 1108 66-67 1.8273771655462703 1108 68-69 1.7486202881929351 1108 70-71 1.7348941675342573 1108 72-73 1.9379560004010692 1108 74-75 1.983676780883453 1108 76-77 1.7606621971606629 1108 78-79 2.2363840354169646 1108 80-81 2.5079044135323123 1108 82-83 2.722418783936874 1108 84-85 2.7410750947139633 1108 86-87 2.9351181568759976 1108 88-89 3.1804424926405446 1108 90-91 3.2954640448464403 1108 92-93 3.277061254203158 1108 94-95 3.4500589639995916 1108 96-97 3.919984900842632 1108 98-99 4.044234075233213 1108 100-101 3.7833794650573545 1109 1 0.21616429399394832 1109 2 0.18838914797332862 1109 3 0.28373113168186137 1109 4 0.2434686258144083 1109 5 0.1503638127755238 1109 6 0.11417579602363759 1109 7 0.254633479909252 1109 8 0.1957209664981292 1109 9 0.2718577689934065 1109 10-11 0.23348732658548954 1109 12-13 0.3420924607960387 1109 14-15 0.4778176313126252 1109 16-17 0.4370876139343025 1109 18-19 0.37181680475631396 1109 20-21 0.3839983161820726 1109 22-23 0.37240514336138375 1109 24-25 0.3487735977605624 1109 26-27 0.3554445786849598 1109 28-29 0.3973781682273625 1109 30-31 0.4989724210103148 1109 32-33 0.5255020160400647 1109 34-35 0.6117310469274457 1109 36-37 0.602933027286987 1109 38-39 0.7203356155937541 1109 40-41 0.6958832787675462 1109 42-43 0.8494014806791128 1109 44-45 0.6391960102830154 1109 46-47 0.824772803541137 1109 48-49 0.7028954149194888 1109 50-51 0.8279263441268299 1109 52-53 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64-65 0.22769818907629968 2316 66-67 0.46353275591222953 2316 68-69 0.44425789827956663 2316 70-71 0.4791968694832107 2316 72-73 0.5703420639131274 2316 74-75 0.8216943613989294 2316 76-77 0.8696986412251739 2316 78-79 1.1705702109386884 2316 80-81 1.0721781051298436 2316 82-83 1.3295443188815383 2316 84-85 1.6839418297383553 2316 86-87 1.645888628764073 2316 88-89 1.903928675968082 2316 90-91 2.120603924303431 2316 92-93 2.07602834776457 2316 94-95 2.7172370631546663 2316 96-97 2.9467253135201865 2316 98-99 3.0969123414933684 2316 100-101 3.5325888420395977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 27.0 5 61.0 6 181.0 7 390.0 8 840.0 9 1540.0 10 2604.0 11 4134.0 12 5824.0 13 8242.0 14 10758.0 15 14480.0 16 18442.0 17 23889.0 18 21688.0 19 16546.0 20 15783.0 21 16912.0 22 18395.0 23 20539.0 24 23690.0 25 27705.0 26 32206.0 27 37979.0 28 45516.0 29 53498.0 30 63189.0 31 74002.0 32 87889.0 33 106037.0 34 131525.0 35 165349.0 36 201001.0 37 214608.0 38 108017.0 39 5055.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18990671209221 19.233298322388716 15.330802747324451 22.245992218194626 2 16.17551620549352 21.168924999683114 38.65380959020445 24.001749204618914 3 19.208770046023105 25.294715736860613 30.082322123714412 25.414192093401876 4 13.277396099597416 17.524064730085424 35.56094022697974 33.63759894333742 5 13.789572007530913 37.72640771810698 32.969237783669534 15.514782490692577 6 31.83715486631761 36.048981505237386 16.85357811416137 15.260285514283634 7 28.83852637380389 33.515145180243955 19.59722657747117 18.049101868480985 8 27.395687828425363 34.133432157891235 19.08961043485973 19.38126957882367 9 27.322963089661112 16.571674727836534 19.55231208288593 36.55305009961642 10-11 25.59239304300452 26.864822575076573 26.068524695053384 21.47425968686552 12-13 26.662612325829638 24.29151977599767 27.029181751665288 22.016686146507407 14-15 23.177462932872153 25.1978866748387 26.09668335914808 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22.18625490120302 25.901887877833733 29.820031055998996 22.091826164964246 42-43 22.184020551611848 25.964782240974614 29.411453532387476 22.43974367502605 44-45 22.01438251874311 26.281941839585155 29.311089198793677 22.392586442878063 46-47 22.223865780876537 26.626948857592996 28.689217276835176 22.459968084695287 48-49 22.411635958202282 26.535312358454412 28.481712705607343 22.571338977735966 50-51 22.447586558593144 26.81604378688104 28.11469983624264 22.621669818283177 52-53 22.900722496206974 26.851709316431798 27.818321186148093 22.429247001213135 54-55 22.235912665669762 26.835943896773916 28.06193894477511 22.86620449278121 56-57 22.290892620149442 27.156608417191674 28.037952431179146 22.51454653147974 58-59 22.37406995638416 26.49952013075249 28.078685886776196 23.04772402608715 60-61 22.205593089882836 26.78902540614676 28.345322145886588 22.660059358083817 62-63 22.08975930518734 27.05474310366126 28.16252838830449 22.692969202846907 64-65 21.874625844522363 26.7474367380294 28.112260896832865 23.265676520615372 66-67 21.90701020145393 27.05157487934335 27.89057295954292 23.1508419596598 68-69 22.042447762253 26.727912669332397 28.034679883842916 23.194959684571685 70-71 21.926287015656722 27.085752476156994 27.73470040638432 23.253260101801963 72-73 22.201489464408567 26.923500874851285 27.64492060455328 23.230089056186863 74-75 22.00483986937421 27.242066320147224 27.34918960695066 23.403904203527908 76-77 21.91222352087827 27.00086471488165 27.829755694290032 23.25715606995005 78-79 22.0789266132634 27.046982841902878 27.72611660664342 23.147973938190308 80-81 21.88432518484474 27.339949301718757 27.65549114712226 23.120234366314246 82-83 21.999019343264067 27.157634868808657 27.51299623574785 23.330349552179428 84-85 21.86953686038405 26.94566564659024 27.552009801856407 23.632787691169302 86-87 22.103556703039196 26.87601380147878 27.470650635260164 23.54977886022186 88-89 21.86756242259535 27.02693285948225 27.302279616484697 23.803225101437707 90-91 21.670477157013107 26.957397894379415 27.663146318394816 23.70897863021266 92-93 21.863545658990844 26.610275252605238 27.641506445788856 23.884672642615058 94-95 21.607197721967243 26.491823218069687 27.647595427463724 24.253383632499343 96-97 21.82584705632683 26.45793146263513 27.54858082233435 24.167640658703686 98-99 21.57346433922163 26.556660996924403 27.30123435673765 24.56864030711632 100-101 21.543539744466486 26.304329854064562 27.697230447999278 24.454899953469678 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1480.0 1 971.5 2 607.0 3 796.5 4 715.0 5 670.5 6 1216.0 7 2666.0 8 3999.0 9 4304.0 10 3610.0 11 2808.0 12 2733.5 13 2905.0 14 3051.0 15 3168.5 16 3332.0 17 3483.5 18 3617.5 19 3821.5 20 4109.0 21 4568.5 22 5120.0 23 5741.5 24 6497.5 25 7670.5 26 9167.0 27 10831.5 28 12397.5 29 14077.0 30 16249.5 31 18557.0 32 21646.5 33 23878.0 34 25493.0 35 27257.0 36 29367.0 37 32298.0 38 34586.0 39 35848.0 40 37560.0 41 42117.5 42 46973.0 43 50797.5 44 53749.5 45 56111.0 46 56914.5 47 58545.5 48 63208.5 49 64609.5 50 63282.5 51 61441.0 52 58623.5 53 57840.5 54 57817.0 55 53741.0 56 47431.5 57 42217.5 58 38523.0 59 34604.0 60 29198.0 61 23748.5 62 19282.0 63 14817.0 64 11259.5 65 9113.5 66 6873.5 67 5405.0 68 4106.0 69 2884.0 70 2275.0 71 1843.5 72 1656.0 73 1522.0 74 1266.0 75 1036.0 76 716.5 77 382.5 78 213.0 79 129.5 80 67.0 81 32.5 82 18.0 83 12.0 84 8.0 85 4.5 86 2.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.334907557861462E-5 2 0.044027607527137164 3 0.0 4 0.0 5 0.10059833201884001 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.819106714685265E-4 26-27 0.011339484528572016 28-29 0.0 30-31 0.0 32-33 5.701416802075316E-4 34-35 0.015108754525499585 36-37 0.0035475482324024187 38-39 0.012321395200040542 40-41 0.007348492767119296 42-43 0.005701416802075316 44-45 0.0026289866365125066 46-47 1.2669815115722924E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 3.167453778930731E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.484199156823804E-4 68-69 1.9004722673584388E-4 70-71 0.0 72-73 6.334907557861462E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.167453778930731E-5 82-83 0.001330330587150907 84-85 0.004972902432921248 86-87 0.0018054486539905166 88-89 0.0 90-91 0.0011402833604150632 92-93 3.1674537789307307E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.18561595889912E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1578555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.72825160096564 #Duplication Level Percentage of deduplicated Percentage of total 1 73.33460730942414 33.53463374103361 2 13.693283775496479 12.523398514586473 3 5.066627185247949 6.950640080859317 4 2.418795142258413 4.4242909134554465 5 1.3892595198193014 3.1764204430666862 6 0.8560233571885969 2.3486670872294066 7 0.5684710595016238 1.8196631345730436 8 0.3961531497106118 1.449231272198549 9 0.30570860896277596 1.2581568168507937 >10 1.702026713730936 14.501623993470098 >50 0.16023514084166668 5.052684485902083 >100 0.10103704913237621 8.570454296620625 >500 0.005828991513870115 1.778330018126939 >1k 0.00152664063458503 0.9999417496023715 >5k 2.7757102447000545E-4 0.9444740490976449 >10k+ 1.3878551223500273E-4 0.6673894033268938 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10516 0.6661788787847113 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8494 0.5380870479647526 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6388 0.4046738947961902 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2057 0.13030904846521027 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1989 0.12600131132586448 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1849 0.11713244074485843 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1589 0.10066168109441863 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.334907557861462E-5 0.0 12-13 0.0 0.0 0.0 6.334907557861462E-5 0.0 14-15 0.0 0.0 0.0 1.5837268894653653E-4 0.0 16-17 0.0 0.0 0.0 1.9004722673584385E-4 0.0 18-19 0.0 0.0 0.0 1.9004722673584385E-4 0.0 20-21 0.0 0.0 0.0 2.2172176452515117E-4 0.0 22-23 0.0 0.0 0.0 6.651652935754536E-4 0.0 24-25 0.0 0.0 0.0 0.0012669815115722923 0.0 26-27 0.0 0.0 0.0 0.0024072648719873557 0.0 28-29 0.0 0.0 0.0 0.00658830386017592 0.0 30-31 0.0 0.0 0.0 0.020873520403153517 0.0 32-33 0.0 0.0 0.0 0.04494616912302707 0.0 34-35 0.0 0.0 0.0 0.07985150976684373 0.0 36-37 0.0 0.0 0.0 0.1391145699706377 0.0 38-39 0.0 0.0 0.0 0.22792997393185538 0.0 40-41 0.0 0.0 0.0 0.32555089939850057 0.0 42-43 0.0 0.0 0.0 0.42269670679830607 0.0 44-45 0.0 0.0 0.0 0.544105210144721 0.0 46-47 0.0 0.0 0.0 0.687780913557019 0.0 48-49 0.0 0.0 0.0 0.8429544741868354 0.0 50-51 0.0 0.0 0.0 1.0186214607663338 0.0 52-53 0.0 0.0 0.0 1.1863698128985054 0.0 54-55 0.0 0.0 0.0 1.370018783000909 0.0 56-57 0.0 0.0 0.0 1.578627288881287 0.0 58-59 0.0 0.0 0.0 1.8097880656676517 0.0 60-61 0.0 0.0 0.0 2.05770467294456 0.0 62-63 0.0 0.0 0.0 2.314933594331525 0.0 64-65 0.0 0.0 0.0 2.5697869253842915 0.0 66-67 0.0 0.0 0.0 2.8700615436269246 0.0 68-69 0.0 0.0 0.0 3.194155414287117 0.0 70-71 0.0 0.0 0.0 3.544412453161277 0.0 72-73 0.0 0.0 0.0 3.8961265207737457 0.0 74-75 0.0 0.0 0.0 4.263234413751817 0.0 76-77 0.0 0.0 0.0 4.65542854065902 0.0 78-79 0.0 0.0 0.0 5.082654706361197 0.0 80-81 0.0 0.0 0.0 5.552894894381254 0.0 82-83 0.0 0.0 0.0 6.035741548441454 0.0 84-85 0.0 0.0 0.0 6.513425252841998 0.0 86-87 0.0 0.0 0.0 7.006566131683723 0.0 88-89 0.0 0.0 0.0 7.530938104785706 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4675 0.0 57.488388 1 GTATCAA 8905 0.0 44.442932 1 TATCAAC 12015 0.0 32.741234 2 ATCAACG 12175 0.0 32.21851 3 TCAACGC 12430 0.0 31.595802 4 CAACGCA 12655 0.0 31.071615 5 AACGCAG 12910 0.0 30.38733 6 ACGCAGA 14660 0.0 26.468348 7 CGCAGAG 14685 0.0 26.358603 8 GTACATG 9325 0.0 25.70958 1 GCAGAGT 15185 0.0 25.084087 9 TACATGG 9485 0.0 24.573784 2 TGGTATC 1945 0.0 24.456467 2 GAGTACT 8770 0.0 24.126085 12-13 GTCTACG 100 0.0016402384 23.783913 1 GTGGTAT 2140 0.0 23.783913 1 GTACGTA 120 1.694734E-4 23.783913 1 ACAATCG 100 0.0016551355 23.746998 8 AGTACTT 8895 0.0 23.680256 12-13 GTACTTT 9420 0.0 22.612587 14-15 >>END_MODULE