FastQCFastQC Report
Thu 26 May 2016
SRR938648_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938648_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588876
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106070.667578841898298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78710.4953816408580657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58550.36849949272315774No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19550.12304295615265132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17650.11108481719152408No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG16240.10221061933089806No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG15910.1001336794060707No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43100.055.103751
GTATCAA83250.045.656561
TATCAAC112350.033.5910642
ATCAACG114750.032.9299283
TCAACGC117200.032.322664
CAACGCA119100.031.7661085
AACGCAG122250.030.9825696
ACGCAGA138850.027.064617
CGCAGAG139800.026.8806978
GCAGAGT144750.025.009659
GTACATG92450.024.451221
TAAGGTG7000.024.4437125
GTAAGGT5650.024.396414
AGGTAAG5550.023.979572
TACATGG94500.023.7906172
AGTACTT82950.022.964212-13
GAGTACT82250.022.5820912-13
ACATGGG96650.022.3761793
GTCCTAC3007.2759576E-1222.2554231
TGGTATC18950.021.8215922
Produced by FastQC (version 0.11.5)