##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938648_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1588876 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.182318192231488 31.0 31.0 34.0 28.0 34.0 2 31.324514940121194 31.0 31.0 34.0 28.0 34.0 3 31.35599002061835 31.0 31.0 34.0 28.0 34.0 4 35.052831687305996 37.0 35.0 37.0 32.0 37.0 5 35.053415747987884 37.0 35.0 37.0 32.0 37.0 6 35.05029341496756 37.0 35.0 37.0 32.0 37.0 7 34.970616964445306 37.0 35.0 37.0 32.0 37.0 8 34.933544845538606 37.0 35.0 37.0 32.0 37.0 9 36.41041906353926 39.0 37.0 39.0 32.0 39.0 10-11 36.25118637326009 39.0 35.0 39.0 32.0 39.0 12-13 36.06317579219524 38.0 35.0 39.0 30.5 39.0 14-15 37.18806722488098 39.0 36.0 41.0 31.5 41.0 16-17 37.13962291582225 39.0 36.0 41.0 31.0 41.0 18-19 37.17221922919095 39.0 36.0 41.0 31.0 41.0 20-21 37.13197411251728 39.0 36.0 41.0 31.0 41.0 22-23 36.98159610945096 39.0 36.0 41.0 31.0 41.0 24-25 36.82640495545279 39.0 36.0 41.0 30.0 41.0 26-27 36.61483117625289 39.0 36.0 41.0 30.0 41.0 28-29 36.36888844692726 39.0 35.0 40.0 30.0 41.0 30-31 36.05743431205455 38.5 35.0 40.0 29.0 41.0 32-33 35.72600221792009 38.0 35.0 40.0 28.0 41.0 34-35 35.39860914256367 38.0 34.0 40.0 27.0 41.0 36-37 35.13455769990861 38.0 34.0 40.0 25.5 41.0 38-39 34.87198529023033 38.0 34.0 40.0 25.0 41.0 40-41 34.5570651831861 38.0 33.0 40.0 24.0 41.0 42-43 34.09149077712799 38.0 33.0 40.0 23.5 41.0 44-45 33.54291933417082 37.5 32.5 40.0 21.0 41.0 46-47 32.9341298502841 37.0 31.5 40.0 16.5 41.0 48-49 32.37184965975948 37.0 31.0 40.0 11.5 41.0 50-51 31.601605474561893 36.0 30.0 38.5 9.0 40.5 52-53 31.596214556705494 36.0 30.0 39.0 8.0 40.0 54-55 31.581397793156924 36.0 30.0 39.0 6.0 40.0 56-57 31.154428979983336 36.0 29.5 39.0 2.0 40.0 58-59 30.708556551927273 35.0 29.0 39.0 2.0 40.0 60-61 30.172528252676734 35.0 28.0 38.5 2.0 40.0 62-63 29.463378199431546 34.5 27.0 38.0 2.0 40.0 64-65 28.812278617085287 34.0 26.0 38.0 2.0 40.0 66-67 28.12539808015226 34.0 25.5 37.0 2.0 39.5 68-69 27.29671289641231 33.0 23.0 36.5 2.0 39.0 70-71 26.47704257600971 32.5 21.0 36.0 2.0 39.0 72-73 25.589170898169524 31.5 18.5 35.0 2.0 38.0 74-75 24.665344872727637 31.0 10.5 35.0 2.0 37.0 76-77 23.734589735133515 30.5 4.5 34.5 2.0 36.5 78-79 22.74908803456028 29.5 2.0 34.0 2.0 36.0 80-81 21.776205946845444 29.0 2.0 34.0 2.0 35.5 82-83 20.778861597758414 27.5 2.0 33.5 2.0 35.0 84-85 19.797901787175334 26.0 2.0 33.0 2.0 35.0 86-87 18.80238829210083 25.0 2.0 32.5 2.0 35.0 88-89 17.70690853156571 22.0 2.0 32.0 2.0 34.0 90-91 16.553448790213963 18.0 2.0 31.0 2.0 34.0 92-93 15.380054516526148 4.5 2.0 31.0 2.0 34.0 94-95 14.105817886354881 2.0 2.0 30.5 2.0 34.0 96-97 12.675210337370569 2.0 2.0 29.0 2.0 34.0 98-99 10.98952246745498 2.0 2.0 27.5 2.0 33.5 100-101 7.7517191398195955 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6064241590688155 1101 2 0.4342915181671998 1101 3 0.5372568675947171 1101 4 0.3473006647988086 1101 5 0.26647859290778086 1101 6 0.46074996250766986 1101 7 0.43657202475208123 1101 8 0.37433571617027894 1101 9 0.5054214601587361 1101 10-11 0.5520428648193203 1101 12-13 0.4679491823994155 1101 14-15 0.5236909046662177 1101 16-17 0.5462512175430376 1101 18-19 0.5262743982568878 1101 20-21 0.723187086915992 1101 22-23 0.7193212552530568 1101 24-25 0.6932317513481152 1101 26-27 0.7748291309913924 1101 28-29 0.9503295350257659 1101 30-31 0.9005387759234011 1101 32-33 1.007353889539992 1101 34-35 1.0321722758247063 1101 36-37 1.1588714815562398 1101 38-39 1.2085015150431104 1101 40-41 0.9745918007535863 1101 42-43 1.3174896073110745 1101 44-45 1.4194203126678175 1101 46-47 1.340508152139158 1101 48-49 1.1440467031385353 1101 50-51 1.4333676738434384 1101 52-53 1.391786820274632 1101 54-55 1.791920260058344 1101 56-57 1.9418703717888413 1101 58-59 1.9268531028272093 1101 60-61 1.9187917111151158 1101 62-63 1.9944509404671855 1101 64-65 2.0494637033625125 1101 66-67 1.9691760560018743 1101 68-69 2.263025787611191 1101 70-71 2.5280938709351304 1101 72-73 2.679801763066699 1101 74-75 2.6391519441176605 1101 76-77 2.5080886277759866 1101 78-79 2.6706441339833624 1101 80-81 3.0701764400798304 1101 82-83 3.357490387227358 1101 84-85 3.497656730236166 1101 86-87 3.601784411294254 1101 88-89 3.8772003506056087 1101 90-91 3.5280943402995177 1101 92-93 3.5288664974079236 1101 94-95 3.4885479183952253 1101 96-97 3.516723056819428 1101 98-99 3.2580736897283042 1101 100-101 2.393582850842204 1102 1 0.6266176901825453 1102 2 0.5860693820450855 1102 3 0.45608154180062854 1102 4 0.38682021001768163 1102 5 0.43534714851016076 1102 6 0.5524956493910551 1102 7 0.5629282025651605 1102 8 0.4361801746172276 1102 9 0.7562010121559695 1102 10-11 0.7056985289167343 1102 12-13 0.6741759727806169 1102 14-15 0.7135537763133328 1102 16-17 0.7967761765533723 1102 18-19 0.7490402285212241 1102 20-21 0.7262759732344577 1102 22-23 0.5310009056902132 1102 24-25 0.6644995475799007 1102 26-27 0.5661706641467106 1102 28-29 0.6516656729937367 1102 30-31 0.36690863987893607 1102 32-33 0.6988407772933982 1102 34-35 0.7035325667603871 1102 36-37 0.839309155009083 1102 38-39 0.9433703197785803 1102 40-41 1.1428361081029408 1102 42-43 1.3167325015581284 1102 44-45 1.0201681568172702 1102 46-47 1.0765255574827322 1102 48-49 0.9502460322587112 1102 50-51 1.075097506802937 1102 52-53 1.071443323982919 1102 54-55 1.0381211826596477 1102 56-57 1.4560513427122608 1102 58-59 1.5719781830068058 1102 60-61 1.3333141885010313 1102 62-63 1.1904709584727122 1102 64-65 1.469160545600598 1102 66-67 1.5091085312280939 1102 68-69 1.533057543991383 1102 70-71 1.2808179552294732 1102 72-73 1.4462190674628452 1102 74-75 1.6036579609244406 1102 76-77 1.7247252178234405 1102 78-79 2.3275858473557456 1102 80-81 2.379012899575077 1102 82-83 2.5987405985456036 1102 84-85 2.663913230547177 1102 86-87 2.7968778645134478 1102 88-89 3.0618979572293092 1102 90-91 3.2257011662226205 1102 92-93 3.184850099920931 1102 94-95 3.257733390700606 1102 96-97 3.3312808819846165 1102 98-99 3.0444192146751803 1102 100-101 2.112676192436835 1103 1 0.40221000165872667 1103 2 0.35555112253462795 1103 3 0.33451569736728715 1103 4 0.43714149183986706 1103 5 0.3048104329594423 1103 6 0.409937796654809 1103 7 0.5198240819259752 1103 8 0.4094293411995906 1103 9 0.38019162505867143 1103 10-11 0.5380296503567124 1103 12-13 0.5287802142721105 1103 14-15 0.520483131378974 1103 16-17 0.5739351278487206 1103 18-19 0.5548332629490744 1103 20-21 0.6809681318884486 1103 22-23 0.6033898972413638 1103 24-25 0.5360426115451347 1103 26-27 0.7306025926330335 1103 28-29 0.5844071131363151 1103 30-31 0.47926306689610954 1103 32-33 0.6715295057494473 1103 34-35 0.6921488965721352 1103 36-37 0.8560066589647803 1103 38-39 1.0231948175078642 1103 40-41 0.9363487327294706 1103 42-43 0.9871419862639357 1103 44-45 0.8926386561110817 1103 46-47 1.2542875347964753 1103 48-49 1.1383715781212018 1103 50-51 1.1179400381685518 1103 52-53 1.145346637498534 1103 54-55 1.157746053988614 1103 56-57 1.254481459572201 1103 58-59 1.5145685075628421 1103 60-61 1.4461672761038997 1103 62-63 1.7474661452300815 1103 64-65 1.7486948433328244 1103 66-67 1.9169348961835482 1103 68-69 2.0503067986067443 1103 70-71 2.0081172187639673 1103 72-73 2.0652387119485454 1103 74-75 2.022581309668695 1103 76-77 2.128597047959918 1103 78-79 2.2609529781519235 1103 80-81 2.4190408848009746 1103 82-83 2.7195504403256052 1103 84-85 2.7079723933533906 1103 86-87 2.917153716676708 1103 88-89 3.1128572852132272 1103 90-91 3.078159603451624 1103 92-93 2.853759548718548 1103 94-95 3.12752602666332 1103 96-97 3.097936106935949 1103 98-99 2.9249933828599026 1103 100-101 2.340911578766546 1104 1 0.6265097535732593 1104 2 0.45290981145036113 1104 3 0.41553234208977585 1104 4 0.4598807525739659 1104 5 0.49596875696327203 1104 6 0.4939217960465001 1104 7 0.42375952728940547 1104 8 0.4169435144799678 1104 9 0.5531217023117705 1104 10-11 0.6063546618824844 1104 12-13 0.6294491702202407 1104 14-15 0.7196423132173919 1104 16-17 0.6455358936986428 1104 18-19 0.7201364008002145 1104 20-21 0.6028088441126229 1104 22-23 0.7335956409680691 1104 24-25 0.7904248949723538 1104 26-27 0.8656751819019064 1104 28-29 0.9681428425005763 1104 30-31 0.8921950709549549 1104 32-33 0.9362338573405538 1104 34-35 1.1752915610344203 1104 36-37 0.9399849369984139 1104 38-39 1.0647110216857882 1104 40-41 1.1080400436845395 1104 42-43 1.3194301566732918 1104 44-45 1.368164716527879 1104 46-47 1.3861818173880067 1104 48-49 1.3500992916782693 1104 50-51 1.4455426008940186 1104 52-53 1.5939952342708779 1104 54-55 1.816164367944996 1104 56-57 2.0202665941551494 1104 58-59 2.08448571766624 1104 60-61 2.2097471039980725 1104 62-63 2.154783466881508 1104 64-65 2.3459256322121256 1104 66-67 2.4397136527441923 1104 68-69 2.8329147560191714 1104 70-71 2.962202766724264 1104 72-73 3.037977917314663 1104 74-75 3.075339613297107 1104 76-77 3.084396604056355 1104 78-79 3.3272301126152826 1104 80-81 3.4983451824267604 1104 82-83 3.662789954460493 1104 84-85 3.4544977756969857 1104 86-87 3.560077485671151 1104 88-89 3.620922369905937 1104 90-91 3.4941815099779205 1104 92-93 3.978747044782809 1104 94-95 4.020178215710276 1104 96-97 3.432164555964368 1104 98-99 3.184812248355202 1104 100-101 2.33910344506247 1105 1 0.4022533808703983 1105 2 0.31008753974431613 1105 3 0.3077879575904987 1105 4 0.2778732702212636 1105 5 0.29843115838999523 1105 6 0.3583552145650728 1105 7 0.2978497638652584 1105 8 0.2553431279083469 1105 9 0.21892904418355386 1105 10-11 0.3259445273296464 1105 12-13 0.3583732955770387 1105 14-15 0.33013280086553465 1105 16-17 0.37102271987591706 1105 18-19 0.37871420581069515 1105 20-21 0.3843410824918223 1105 22-23 0.32535409348601974 1105 24-25 0.5106187785724572 1105 26-27 0.4237892006028119 1105 28-29 0.5008582092205458 1105 30-31 0.41193961486520436 1105 32-33 0.5675611031672361 1105 34-35 0.5793311348093226 1105 36-37 0.6041906967162589 1105 38-39 0.751305305805964 1105 40-41 0.9026776078848258 1105 42-43 0.9237447143825577 1105 44-45 0.8624134673934307 1105 46-47 0.9303062775078601 1105 48-49 1.056184058465 1105 50-51 0.8680887927152376 1105 52-53 0.9898836764769214 1105 54-55 1.3039470110786233 1105 56-57 1.4049489428766258 1105 58-59 1.487267439205155 1105 60-61 1.6063142882522463 1105 62-63 1.539869324637099 1105 64-65 1.5584341631901673 1105 66-67 1.6466639713170643 1105 68-69 1.7747446943968654 1105 70-71 1.8354149975314336 1105 72-73 1.964447113634261 1105 74-75 2.0895844785201056 1105 76-77 2.1402776236888137 1105 78-79 2.4490076446344737 1105 80-81 2.400502959440697 1105 82-83 2.779930868782074 1105 84-85 2.792470217524432 1105 86-87 2.981708309776085 1105 88-89 3.2973018975512858 1105 90-91 2.9997721027376727 1105 92-93 3.3794863131860584 1105 94-95 3.302385459942835 1105 96-97 3.3602552608099625 1105 98-99 2.8216628354359408 1105 100-101 1.9782405833365466 1106 1 0.1593739016272835 1106 2 0.1672530878189562 1106 3 -0.07368454206381614 1106 4 0.11995927594362854 1106 5 0.07363032449825369 1106 6 0.07758719743342368 1106 7 0.13170037953405966 1106 8 -0.11759970867085201 1106 9 0.13589760660666172 1106 10-11 0.22764014266162746 1106 12-13 0.2705522986651374 1106 14-15 0.4080082560137157 1106 16-17 -0.06955894797848572 1106 18-19 0.3509435189491015 1106 20-21 0.3584844111806049 1106 22-23 0.21940658753851494 1106 24-25 0.27535068875890545 1106 26-27 0.3153735240053095 1106 28-29 0.3640505042310238 1106 30-31 0.1675221159022442 1106 32-33 0.3453254082042605 1106 34-35 0.3375422384749669 1106 36-37 0.4178740376723269 1106 38-39 0.38274695997488806 1106 40-41 0.5078374903174279 1106 42-43 0.3643079458548826 1106 44-45 0.7161019847183141 1106 46-47 0.7220630874464931 1106 48-49 0.700492180045849 1106 50-51 0.5394638277266388 1106 52-53 0.42882681087460384 1106 54-55 0.588979859747834 1106 56-57 1.164460697842923 1106 58-59 1.0967529577815824 1106 60-61 1.3640449823465808 1106 62-63 1.28899217830541 1106 64-65 1.3952595954701401 1106 66-67 1.5130374285996133 1106 68-69 1.4481144041302088 1106 70-71 1.5201406322570676 1106 72-73 1.4904126305088674 1106 74-75 1.7027576280568937 1106 76-77 2.045794711024083 1106 78-79 2.4399670810484544 1106 80-81 2.3151625422820956 1106 82-83 2.526103901318745 1106 84-85 2.453676701458189 1106 86-87 2.2392959446364458 1106 88-89 1.9464945967439853 1106 90-91 2.0483783831621345 1106 92-93 1.9873547483272187 1106 94-95 2.1893906469480253 1106 96-97 1.9551437738802964 1106 98-99 1.9079516626338577 1106 100-101 1.3796043437912147 1107 1 0.34463840488486497 1107 2 0.23795562426543526 1107 3 0.20801999984833586 1107 4 0.13397779273031318 1107 5 0.20766505126654522 1107 6 0.22116295311990086 1107 7 0.2631591739686314 1107 8 0.291118351950324 1107 9 0.3703209572929822 1107 10-11 0.408980999088989 1107 12-13 0.34958115451060223 1107 14-15 0.5456897090930894 1107 16-17 0.3338799580555829 1107 18-19 0.5816837747758612 1107 20-21 0.5069309546098424 1107 22-23 0.46943204552244566 1107 24-25 0.4885322093798763 1107 26-27 0.5413365112139701 1107 28-29 0.49860893809741214 1107 30-31 0.43530010150030307 1107 32-33 0.5511230743825521 1107 34-35 0.6450679302567153 1107 36-37 0.5322957257964376 1107 38-39 0.6509987758045597 1107 40-41 0.5460644716239997 1107 42-43 0.7718632520261011 1107 44-45 0.5897301111146049 1107 46-47 0.7062677072912535 1107 48-49 0.45782823582481313 1107 50-51 0.6889021934279818 1107 52-53 0.7885928052426721 1107 54-55 0.7032591909821839 1107 56-57 0.9802087988851405 1107 58-59 0.9476990595927361 1107 60-61 1.1885963503235537 1107 62-63 1.1880699758261137 1107 64-65 1.6361581064378576 1107 66-67 1.8055158932234079 1107 68-69 1.9072184909159589 1107 70-71 2.1518404982129873 1107 72-73 2.1883801481995704 1107 74-75 2.400101684916521 1107 76-77 2.4833862818336456 1107 78-79 2.7683132472838956 1107 80-81 2.616142934438958 1107 82-83 2.891003056529321 1107 84-85 2.8194985786987523 1107 86-87 2.712173320281991 1107 88-89 2.7469154328049576 1107 90-91 2.530353610985113 1107 92-93 2.664962633028532 1107 94-95 2.2946786338969094 1107 96-97 2.2307545057194016 1107 98-99 1.839563724522737 1107 100-101 1.3468456335238557 1108 1 0.16547366498511096 1108 2 0.13651001755975756 1108 3 0.13134929361518033 1108 4 0.09806883711770809 1108 5 0.04033331781415228 1108 6 0.12293704036390807 1108 7 -0.03202417451307582 1108 8 0.07889494266250807 1108 9 0.12059336227015649 1108 10-11 0.20143963339661042 1108 12-13 0.12331692965557295 1108 14-15 -0.05076324590294945 1108 16-17 0.21871809634695438 1108 18-19 0.12548649510497967 1108 20-21 0.10068577152712521 1108 22-23 0.12821856772146134 1108 24-25 0.12730194877467937 1108 26-27 0.2606517515416016 1108 28-29 0.24592143045678938 1108 30-31 0.3797434386074485 1108 32-33 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0.05619652705895106 2316 92-93 0.03686849071127618 2316 94-95 -0.1626366107952748 2316 96-97 0.02094423937344736 2316 98-99 -0.2001363270152865 2316 100-101 0.08926858701415519 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4536.0 3 1627.0 4 1762.0 5 1985.0 6 2459.0 7 3284.0 8 4224.0 9 5862.0 10 7599.0 11 9820.0 12 12705.0 13 15556.0 14 18408.0 15 22132.0 16 26701.0 17 31559.0 18 29131.0 19 26295.0 20 28535.0 21 31434.0 22 35036.0 23 39041.0 24 43208.0 25 48259.0 26 53493.0 27 59468.0 28 65706.0 29 73215.0 30 81563.0 31 90330.0 32 101225.0 33 113675.0 34 126845.0 35 136322.0 36 129423.0 37 86814.0 38 19378.0 39 261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.649075590328316 18.32797590166586 14.645247048358401 22.37770145964742 2 15.785769192753207 20.32146834642933 39.689209071524225 24.203553389293244 3 19.265957426714426 25.09770045829653 29.473333614561227 26.163008500427825 4 12.894077526916181 16.715832237208712 35.95840750145923 34.43168273441588 5 13.898092453259153 37.547855560754755 32.80465232569051 15.749399660295582 6 31.987811144528237 35.49516123344467 17.241600797980865 15.275426824046226 7 29.078642913134228 33.33280864848528 19.70804436315327 17.880504075227215 8 27.540260657732933 33.49796280473715 19.385057293952386 19.576719243577532 9 27.873784127691696 16.446079773941722 19.558725475566703 36.12141062279988 10-11 25.60117437763872 26.631304795134003 26.164783718889804 21.602737108337468 12-13 26.860171816405572 24.22236093784774 26.940473683431847 21.97699356231485 14-15 23.4453045616571 25.025811910496888 26.132954003175495 25.395929524670517 16-17 22.231245315991366 28.63763140011451 26.51631254695812 22.614810736936004 18-19 22.26795900624324 26.246807396201422 30.02042259151606 21.46481100603927 20-21 23.041847621470044 25.29663156489756 30.12156133407709 21.539959479555307 22-23 22.735660537012134 25.254604795938413 30.07002891061382 21.939705756435636 24-25 22.50090746729891 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28.354400976725348 22.644592774586762 50-51 22.550596647819635 26.80183235321425 28.03525596136385 22.61231503760227 52-53 22.85226466251548 26.746794947449253 27.898332129520785 22.502608260514474 54-55 22.21070703033412 26.77881127761456 28.174807117039002 22.835674575012312 56-57 22.357279513516797 26.996991581526757 28.137162984902513 22.50856592005393 58-59 22.45708932149501 26.270955746636837 28.252363663142088 23.01959126872607 60-61 22.23075104981685 26.605221341959094 28.5052301225602 22.658797485663857 62-63 22.19217953685027 26.804258039215934 28.299191994790597 22.704370429143204 64-65 21.940909148129965 26.598656797210367 28.293679267847477 23.16675478681219 66-67 22.02695035059247 26.82330586309695 28.048341825535516 23.101401960775057 68-69 22.167878670196043 26.480863541106416 28.24601316560929 23.10524462308825 70-71 22.07394174698613 26.777854991892518 27.91718282623803 23.231020434883323 72-73 22.359428208146333 26.651738224574967 27.83223914042387 23.15659442685483 74-75 22.126279599239872 26.99044049749446 27.62379609248175 23.259483810783916 76-77 22.23062141508846 26.662646803169988 27.794460770768964 23.31227101097259 78-79 22.473474523488733 26.73583605803078 27.55650265608826 23.23418676239222 80-81 22.385276461335195 27.034960756407735 27.396081117062295 23.183681665194783 82-83 22.44332979677316 26.847181258727964 27.244281048580127 23.465207895918752 84-85 22.421064160173522 26.526867108068288 27.347020602029172 23.705048129729022 86-87 22.71843114201726 26.480961238953704 27.18701782355446 23.613589795474578 88-89 22.551857523019375 26.48404341538108 27.161901224814816 23.802197836784732 90-91 22.49957899673742 26.522135328547797 27.232851789539286 23.74543388517549 92-93 22.727747676069814 26.081080740837727 27.271465511163928 23.919706071928534 94-95 22.582162077433512 25.94868737712751 27.1929139831178 24.27623656232118 96-97 22.946249108474355 25.760228230564742 27.0134315852821 24.280091075678804 98-99 22.710502123298944 25.79377830763648 26.635292681015443 24.86042688804913 100-101 23.713388547867826 24.82480003478478 26.85838625093815 24.603425166409245 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1159.0 1 840.5 2 642.5 3 760.5 4 639.0 5 617.5 6 1068.5 7 2274.0 8 3597.5 9 3959.5 10 3374.0 11 2739.5 12 2574.0 13 2644.5 14 2867.0 15 3089.5 16 3261.5 17 3397.5 18 3520.0 19 3770.5 20 4105.0 21 4558.5 22 5137.5 23 5856.0 24 6671.5 25 7834.5 26 9252.0 27 10861.5 28 12456.5 29 14081.0 30 16116.0 31 18604.0 32 21526.0 33 23521.5 34 25400.0 35 27254.0 36 29327.0 37 32088.0 38 34137.0 39 35578.5 40 37732.5 41 41589.0 42 46076.0 43 50158.0 44 52898.0 45 55095.5 46 56485.5 47 58119.0 48 61564.0 49 63219.5 50 62434.0 51 60945.0 52 59301.5 53 58581.0 54 57484.0 55 54020.5 56 48864.0 57 43979.0 58 40080.5 59 35979.0 60 30681.5 61 25413.5 62 21115.0 63 16730.0 64 13101.0 65 10524.5 66 8156.5 67 6355.0 68 4876.0 69 3557.0 70 2802.0 71 2296.0 72 1891.0 73 1626.5 74 1356.0 75 1112.5 76 787.0 77 511.0 78 323.5 79 189.0 80 110.5 81 62.5 82 33.5 83 20.5 84 16.0 85 9.5 86 5.0 87 5.5 88 5.5 89 3.0 90 1.5 91 1.0 92 1.0 93 2.5 94 2.0 95 0.5 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37938769293513147 2 0.030021222549777328 3 0.037951356808209075 4 0.04437098930313001 5 0.06451101281660745 6 0.05462981378030759 7 0.03908423313084218 8 0.0417276112169861 9 0.038580732543005244 10-11 0.039682140078898545 12-13 0.031122630085670625 14-15 0.029077158947583073 16-17 0.022626057665922322 18-19 0.02762959475755188 20-21 0.03411216482595243 22-23 0.03348278909115626 24-25 0.04182201757720552 26-27 0.03637791747121864 28-29 0.031783474607206604 30-31 0.034175102399432056 32-33 0.02888834622714422 34-35 0.030493254350874452 36-37 0.03297928850331933 38-39 0.015891737303603302 40-41 0.013657453445076897 42-43 0.01422389160639345 44-45 0.013216890430719578 46-47 0.021965213144386347 48-49 0.019038615977584154 50-51 0.013122484070500153 52-53 0.010919668998713557 54-55 0.009660917529121215 56-57 0.010542043557835855 58-59 0.005129412238588788 60-61 0.009220354514763895 62-63 0.013248359217459387 64-65 0.008559509993227918 66-67 0.004122411062914917 68-69 0.007269289736895768 70-71 0.013846266165515748 72-73 0.011706388667208769 74-75 0.012272826828525324 76-77 0.006230819774482087 78-79 0.010542043557835855 80-81 0.01315395285723996 82-83 0.01463298583401096 84-85 0.009377698448462939 86-87 0.003241285034200277 88-89 0.016898738479277177 90-91 0.02570999876642356 92-93 0.011832263814168003 94-95 0.021335837409590174 96-97 0.01932183505824243 98-99 0.01211548289482628 100-101 0.12430170762224364 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1588876.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.541406639629756 #Duplication Level Percentage of deduplicated Percentage of total 1 75.56262439602592 36.67916077565103 2 12.762835818660227 12.39052006696836 3 4.632534076567686 6.7460916114784135 4 2.2258262111146934 4.321789408914588 5 1.1762843956479216 2.854924958649845 6 0.7549643035223709 2.198821755341056 7 0.5184467190459228 1.7616293107133012 8 0.3529260299219896 1.370522074572275 9 0.2730706266495526 1.1929709096581007 >10 1.5026270741755154 13.66026956850576 >50 0.1432789901490045 4.802883079975262 >100 0.08776949727923905 7.907611630360677 >500 0.005108895930035469 1.6732532476096722 >1k 0.00130997331539371 0.8922660777707274 >5k 2.61994663078742E-4 0.8728081658695128 >10k+ 1.30997331539371E-4 0.6744773579613815 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10607 0.667578841898298 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7871 0.4953816408580657 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5855 0.36849949272315774 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1955 0.12304295615265132 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1765 0.11108481719152408 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1624 0.10221061933089806 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 1591 0.1001336794060707 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.293757347961704E-5 0.0 18-19 0.0 0.0 0.0 9.440636021942555E-5 0.0 20-21 0.0 0.0 0.0 1.888127204388511E-4 0.0 22-23 0.0 0.0 0.0 8.811260287146385E-4 0.0 24-25 0.0 0.0 0.0 0.001762252057429277 0.0 26-27 0.0 0.0 0.0 0.0029580659535420007 0.0 28-29 0.0 0.0 0.0 0.0070175394429773 0.0 30-31 0.0 0.0 0.0 0.01916449112454339 0.0 32-33 0.0 0.0 0.0 0.04279754996613959 0.0 34-35 0.0 0.0 0.0 0.0790810610771388 0.0 36-37 0.0 0.0 0.0 0.13541018934139606 0.0 38-39 0.0 0.0 0.0 0.22446685581505416 0.0 40-41 0.0 0.0 0.0 0.324097034633288 0.0 42-43 0.0 0.0 0.0 0.4248915585608946 0.0 44-45 3.146878673980852E-5 0.0 0.0 0.548091858647245 0.0 46-47 6.293757347961704E-5 0.0 0.0 0.6918412764746903 0.0 48-49 6.293757347961704E-5 0.0 0.0 0.8484299592919775 0.0 50-51 6.293757347961704E-5 0.0 0.0 1.016504749269295 0.0 52-53 6.293757347961704E-5 0.0 0.0 1.1814326605726313 0.0 54-55 6.293757347961704E-5 0.0 0.0 1.3618117461652135 0.0 56-57 6.293757347961704E-5 0.0 0.0 1.5775932168400808 0.0 58-59 6.293757347961704E-5 0.0 0.0 1.806969203386545 0.0 60-61 6.293757347961704E-5 0.0 0.0 2.057901308849778 0.0 62-63 6.293757347961704E-5 3.146878673980852E-5 0.0 2.306882349535143 0.0 64-65 6.293757347961704E-5 6.293757347961704E-5 0.0 2.561968334848031 0.0 66-67 6.293757347961704E-5 6.293757347961704E-5 0.0 2.8534322376321377 0.0 68-69 6.293757347961704E-5 6.293757347961704E-5 0.0 3.1751691132599396 0.0 70-71 6.293757347961704E-5 6.293757347961704E-5 0.0 3.519437640193445 0.0 72-73 6.293757347961704E-5 6.293757347961704E-5 0.0 3.87355589737651 0.0 74-75 6.293757347961704E-5 6.293757347961704E-5 0.0 4.230758095660077 0.0 76-77 6.293757347961704E-5 6.293757347961704E-5 0.0 4.6121283221598155 0.0 78-79 6.293757347961704E-5 6.293757347961704E-5 0.0 5.026163149295477 0.0 80-81 6.293757347961704E-5 6.293757347961704E-5 0.0 5.470061855047216 0.0 82-83 6.293757347961704E-5 6.293757347961704E-5 0.0 5.9274921390970725 0.0 84-85 6.293757347961704E-5 6.293757347961704E-5 0.0 6.370195030952699 3.146878673980852E-5 86-87 6.293757347961704E-5 6.293757347961704E-5 0.0 6.819349024089986 6.293757347961704E-5 88-89 6.293757347961704E-5 6.293757347961704E-5 0.0 7.2915381691208125 6.293757347961704E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4310 0.0 55.10375 1 GTATCAA 8325 0.0 45.65656 1 TATCAAC 11235 0.0 33.591064 2 ATCAACG 11475 0.0 32.929928 3 TCAACGC 11720 0.0 32.32266 4 CAACGCA 11910 0.0 31.766108 5 AACGCAG 12225 0.0 30.982569 6 ACGCAGA 13885 0.0 27.06461 7 CGCAGAG 13980 0.0 26.880697 8 GCAGAGT 14475 0.0 25.00965 9 GTACATG 9245 0.0 24.45122 1 TAAGGTG 700 0.0 24.443712 5 GTAAGGT 565 0.0 24.39641 4 AGGTAAG 555 0.0 23.97957 2 TACATGG 9450 0.0 23.790617 2 AGTACTT 8295 0.0 22.9642 12-13 GAGTACT 8225 0.0 22.58209 12-13 ACATGGG 9665 0.0 22.376179 3 GTCCTAC 300 7.2759576E-12 22.255423 1 TGGTATC 1895 0.0 21.821592 2 >>END_MODULE