FastQCFastQC Report
Thu 26 May 2016
SRR938648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588876
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104480.6575717677150388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86940.5471792638317905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63050.3968214007889854No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21400.13468640724638045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20310.1278262117371022No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG18390.11574219762901573No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48650.058.411821
GTATCAA87550.041.6857341
TATCAAC116400.031.3051222
ATCAACG117550.030.911223
TCAACGC120100.030.2944554
CAACGCA122850.029.616315
TGGTATC19950.029.529212
AACGCAG127300.028.6556426
GTGGTAT21950.026.6288851
GTAAGGT6150.024.7145234
ACGCAGA148050.024.5110727
CGCAGAG149250.024.2185258
TATCACG1000.001652163623.7543432
TAAGGTG7400.023.7491115
GTACATG95000.022.959981
GAGTACT86350.022.8002512-13
GCAGAGT155150.022.6546469
AGTACTT86750.022.14758512-13
TACATGG98600.021.6342812
GTACTTT90400.021.59487714-15
Produced by FastQC (version 0.11.5)