##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938648_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1588876 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74515128933913 31.0 31.0 34.0 30.0 34.0 2 31.997705296070933 31.0 31.0 34.0 30.0 34.0 3 32.03570952044087 33.0 31.0 34.0 30.0 34.0 4 35.72013738013539 37.0 35.0 37.0 33.0 37.0 5 35.61436260601834 37.0 35.0 37.0 33.0 37.0 6 35.65750568326288 37.0 35.0 37.0 33.0 37.0 7 35.576162016419154 37.0 35.0 37.0 33.0 37.0 8 35.55569282939638 37.0 35.0 37.0 33.0 37.0 9 37.06404401602139 39.0 37.0 39.0 33.0 39.0 10-11 36.998068131182045 39.0 37.0 39.0 33.0 39.0 12-13 36.864983485180716 39.0 37.0 39.0 32.5 39.0 14-15 37.80823487798922 40.0 37.0 41.0 32.5 41.0 16-17 37.91907235051697 40.0 37.0 41.0 33.0 41.0 18-19 38.09122738338297 40.0 37.0 41.0 33.0 41.0 20-21 38.06089996953821 40.0 37.0 41.0 33.0 41.0 22-23 37.92854067907125 40.0 37.0 41.0 32.5 41.0 24-25 37.790560748604676 40.0 37.0 41.0 32.0 41.0 26-27 37.55728326187821 39.5 36.5 41.0 32.0 41.0 28-29 37.39282927050317 39.0 36.0 41.0 31.5 41.0 30-31 37.08353452377655 39.0 36.0 40.5 31.0 41.0 32-33 36.91626313192471 39.0 35.0 40.0 31.0 41.0 34-35 36.73431218043447 39.0 35.0 40.0 30.0 41.0 36-37 36.5650960175621 39.0 35.0 40.0 30.0 41.0 38-39 36.32313912476493 38.0 35.0 40.0 30.0 41.0 40-41 36.059843247679495 38.0 35.0 40.0 30.0 41.0 42-43 35.72817608170807 38.0 35.0 40.0 28.5 41.0 44-45 35.332738678159906 38.0 34.0 40.0 27.0 41.0 46-47 34.77105135957746 38.0 33.5 40.0 25.0 41.0 48-49 34.24774054111208 38.0 33.0 40.0 24.0 41.0 50-51 33.792304748765794 37.5 33.0 40.0 22.5 41.0 52-53 33.16211176957799 37.0 32.0 40.0 20.0 41.0 54-55 32.70604156649103 36.5 31.0 39.0 18.0 40.5 56-57 32.29398392322623 36.0 31.0 39.0 15.5 40.0 58-59 31.74509055458072 35.5 30.5 39.0 11.0 40.0 60-61 31.247770436459483 35.0 30.0 38.5 8.0 40.0 62-63 30.73669751446935 35.0 29.0 38.0 7.0 40.0 64-65 30.066890367782005 34.0 28.0 38.0 2.0 40.0 66-67 29.386712997112426 34.0 27.5 37.0 2.0 39.5 68-69 28.656759558329284 33.5 26.0 36.5 2.0 39.0 70-71 27.882463137463212 33.0 25.5 36.0 2.0 39.0 72-73 27.3031510954914 32.0 24.5 35.0 2.0 38.0 74-75 26.579944249897412 32.0 22.5 35.0 2.0 37.5 76-77 25.460433665056303 30.5 21.5 34.0 2.0 36.0 78-79 25.959128339782335 31.0 22.0 34.5 2.0 36.0 80-81 25.56481374254504 31.0 20.5 34.5 2.0 36.0 82-83 24.877961842207952 31.0 18.5 34.0 2.0 35.5 84-85 24.096095919379486 30.5 13.0 34.0 2.0 35.0 86-87 23.17933778343936 30.0 4.5 34.0 2.0 35.0 88-89 22.19485063655062 29.0 2.0 34.0 2.0 35.0 90-91 21.327897834695722 29.0 2.0 33.5 2.0 35.0 92-93 20.22943577724127 27.0 2.0 33.0 2.0 34.5 94-95 19.04268835327615 25.5 2.0 32.5 2.0 34.0 96-97 17.66106071210088 22.0 2.0 32.0 2.0 34.0 98-99 15.92061809732163 9.5 2.0 32.0 2.0 34.0 100-101 11.929222922367762 2.0 2.0 26.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37427504938405676 1101 2 0.28912575547636976 1101 3 0.31379710735816246 1101 4 0.11065342749395768 1101 5 0.23903837097564207 1101 6 0.33003810813391965 1101 7 0.2836740718570496 1101 8 0.2147684996014263 1101 9 0.3802745966869452 1101 10-11 0.4489460218014685 1101 12-13 0.4043522863785469 1101 14-15 0.6713597386848065 1101 16-17 0.46070710527064307 1101 18-19 0.5551917596707625 1101 20-21 0.5707101762118967 1101 22-23 0.5645585380974865 1101 24-25 0.4716608642988831 1101 26-27 0.5734657469998439 1101 28-29 0.5795607035220982 1101 30-31 0.7390171931736447 1101 32-33 0.7219564496354991 1101 34-35 0.6919183580197483 1101 36-37 0.7018804283724407 1101 38-39 0.6870653520994026 1101 40-41 0.8281868359764175 1101 42-43 0.8549474911354409 1101 44-45 0.9638294117370734 1101 46-47 1.1504150116966443 1101 48-49 1.2581390702821835 1101 50-51 1.2899028074361922 1101 52-53 1.4695632651919368 1101 54-55 1.5630822006339002 1101 56-57 1.4592850419380028 1101 58-59 1.7056852651422503 1101 60-61 1.4040417177133513 1101 62-63 1.5159775199380086 1101 64-65 1.5645746593901428 1101 66-67 1.7799716986639567 1101 68-69 1.985195819140511 1101 70-71 2.1652184583846896 1101 72-73 2.115609359921958 1101 74-75 2.1199675942608565 1101 76-77 2.4305619966440055 1101 78-79 2.323744773390171 1101 80-81 2.316321094689325 1101 82-83 2.6601342453534578 1101 84-85 2.6499931399267638 1101 86-87 2.580199906621683 1101 88-89 2.597914736895344 1101 90-91 2.8432572752825997 1101 92-93 3.3009582077670387 1101 94-95 3.1233190734917393 1101 96-97 2.6240098769658715 1101 98-99 2.798260047375889 1101 100-101 2.6402511961124002 1102 1 0.26056601580205196 1102 2 0.16701212005029475 1102 3 0.21202907129534054 1102 4 0.21958323101045352 1102 5 0.1709975357974045 1102 6 0.24885428916631724 1102 7 0.30555693562573794 1102 8 0.23562530712265328 1102 9 0.22118653703736157 1102 10-11 0.21277796373728108 1102 12-13 0.3323825495779502 1102 14-15 0.3737953381855661 1102 16-17 0.29355356406217226 1102 18-19 0.28989110537285256 1102 20-21 0.31184026979048696 1102 22-23 0.29617809730986266 1102 24-25 0.2133529228580997 1102 26-27 0.3278544933022829 1102 28-29 0.33438523051299995 1102 30-31 0.39791071870614303 1102 32-33 0.42127727886042976 1102 34-35 0.5830848104697139 1102 36-37 0.3912395739430039 1102 38-39 0.340661766200661 1102 40-41 0.6561788394513357 1102 42-43 0.7429609540287672 1102 44-45 0.6540932825049381 1102 46-47 0.7701766447155194 1102 48-49 0.9385979766688095 1102 50-51 0.9693352531385315 1102 52-53 0.7793570266728906 1102 54-55 0.7511748522592967 1102 56-57 0.9825862656829756 1102 58-59 1.1851514004328436 1102 60-61 1.1458419631256227 1102 62-63 1.1915281809091205 1102 64-65 1.259011104972764 1102 66-67 1.298729883454378 1102 68-69 1.488645665333184 1102 70-71 1.7312931238952096 1102 72-73 1.7235454621377038 1102 74-75 1.9553967866732975 1102 76-77 1.892998914608846 1102 78-79 1.819432870086164 1102 80-81 1.9366206286972059 1102 82-83 2.0694613271718403 1102 84-85 2.1399453112267963 1102 86-87 2.1605166130175313 1102 88-89 2.0959406807093615 1102 90-91 2.3489070556329565 1102 92-93 2.416861513391055 1102 94-95 2.3149276967321377 1102 96-97 2.6098784495545964 1102 98-99 2.457858101872942 1102 100-101 2.353443602082857 1103 1 0.09432342621110124 1103 2 0.16296067171446538 1103 3 0.19880983593139945 1103 4 0.08741597449697736 1103 5 0.05238929112127266 1103 6 0.1430598647438046 1103 7 0.21763475370477892 1103 8 0.17108244933996275 1103 9 0.3087812979930291 1103 10-11 0.34511240572006585 1103 12-13 0.41165182166091796 1103 14-15 0.48400067900840327 1103 16-17 0.34607354716600724 1103 18-19 0.2815702498156938 1103 20-21 0.36325269742388855 1103 22-23 0.4918860640642819 1103 24-25 0.27490748516809305 1103 26-27 0.47344716772151685 1103 28-29 0.5202566683621015 1103 30-31 0.4798119070743567 1103 32-33 0.312736649702515 1103 34-35 0.39127558569289533 1103 36-37 0.5149249671840792 1103 38-39 0.3982011744799294 1103 40-41 0.6232006368843273 1103 42-43 0.39235449163468417 1103 44-45 0.4392014588396691 1103 46-47 0.46161694174193 1103 48-49 0.4382184309036177 1103 50-51 0.4834776766251139 1103 52-53 0.5694960184170981 1103 54-55 0.5604355372641123 1103 56-57 0.7792080380960584 1103 58-59 0.8885811736552114 1103 60-61 0.6491516948643721 1103 62-63 0.8682223720441975 1103 64-65 1.0773173733081443 1103 66-67 0.7491839097669768 1103 68-69 0.8551100362949597 1103 70-71 1.1317260686220685 1103 72-73 1.1944420627513104 1103 74-75 1.1077272671712883 1103 76-77 1.2141674136231053 1103 78-79 1.270240379764001 1103 80-81 1.296891608156944 1103 82-83 1.4990189073146816 1103 84-85 1.6836563962025721 1103 86-87 1.591206459647065 1103 88-89 1.7352061766401512 1103 90-91 1.7573232030465213 1103 92-93 1.6755783243726299 1103 94-95 1.4763379312628508 1103 96-97 1.8476378090450574 1103 98-99 1.8550645214860761 1103 100-101 1.6771320455899996 1104 1 0.24806665240000925 1104 2 0.26054489287337645 1104 3 0.2735137925642448 1104 4 0.18638550254399888 1104 5 0.18185808701902317 1104 6 0.14974757862569987 1104 7 0.2045461320832871 1104 8 0.171678947056769 1104 9 0.11830584575658776 1104 10-11 0.3487796116708708 1104 12-13 0.35705917681735144 1104 14-15 0.4378363328138235 1104 16-17 0.35827923404826834 1104 18-19 0.40271963742736716 1104 20-21 0.3644331427817846 1104 22-23 0.40280027433046683 1104 24-25 0.46007111785394983 1104 26-27 0.5437748391250921 1104 28-29 0.6134760470078433 1104 30-31 0.6425961709035235 1104 32-33 0.7442542085503732 1104 34-35 0.613167199547739 1104 36-37 0.552858493862395 1104 38-39 0.6032105372055483 1104 40-41 0.8127163774476855 1104 42-43 0.6804656572524905 1104 44-45 0.6701752672007473 1104 46-47 0.8701523589784301 1104 48-49 1.0545644615915037 1104 50-51 1.076063276446753 1104 52-53 1.0407639119644045 1104 54-55 1.208495424748989 1104 56-57 1.350854574810377 1104 58-59 1.525764944044937 1104 60-61 1.4356095725653581 1104 62-63 1.455004484642739 1104 64-65 1.6322553297940807 1104 66-67 1.5820288975066603 1104 68-69 1.8110758808947551 1104 70-71 1.9883591712185726 1104 72-73 2.072693409023774 1104 74-75 2.1872676169566674 1104 76-77 2.2017426540954794 1104 78-79 2.206218198612518 1104 80-81 2.464246129709302 1104 82-83 2.5908550092316354 1104 84-85 2.667605587590124 1104 86-87 2.5238681055452012 1104 88-89 2.4998394726554665 1104 90-91 2.454615192000979 1104 92-93 2.8094252510990145 1104 94-95 2.599802623285836 1104 96-97 2.7415785955730705 1104 98-99 3.200359738024714 1104 100-101 2.6501370172655854 1105 1 0.03796298544939347 1105 2 0.033894864727372465 1105 3 0.1372796776189702 1105 4 0.10936260216091398 1105 5 0.06397117914362127 1105 6 0.029100868220645282 1105 7 0.10453068914494423 1105 8 0.09634729750187176 1105 9 0.13082458900030502 1105 10-11 0.17101644656592896 1105 12-13 0.12027907927775772 1105 14-15 0.19722414484835582 1105 16-17 0.20360699796061255 1105 18-19 0.1836839742029781 1105 20-21 0.2148433769664706 1105 22-23 0.33624868246241846 1105 24-25 0.16860184681045354 1105 26-27 0.18344376245339333 1105 28-29 0.21371566163180944 1105 30-31 0.28478204410205876 1105 32-33 0.287549233433424 1105 34-35 0.3444862257459391 1105 36-37 0.485893429774201 1105 38-39 0.4140544688780139 1105 40-41 0.49405259014177716 1105 42-43 0.3245146462381925 1105 44-45 0.2644743321557357 1105 46-47 0.5044349348605479 1105 48-49 0.5249862530719867 1105 50-51 0.4141054605763941 1105 52-53 0.4681845925496404 1105 54-55 0.6720401270738989 1105 56-57 0.9054353986662349 1105 58-59 0.8138118606879345 1105 60-61 0.8998952868510806 1105 62-63 0.8566603661605328 1105 64-65 1.148920003823541 1105 66-67 1.1381490336037814 1105 68-69 1.3635059019668923 1105 70-71 1.324292896105117 1105 72-73 1.5045900509319203 1105 74-75 1.3554773320593583 1105 76-77 1.3798682300921712 1105 78-79 1.5624145530827498 1105 80-81 1.7560940018218467 1105 82-83 1.9647173379271017 1105 84-85 1.9376677080550024 1105 86-87 1.760785196892673 1105 88-89 1.8416253239349878 1105 90-91 1.8195280888471324 1105 92-93 1.6534453468452313 1105 94-95 1.649267351116503 1105 96-97 1.618955438139782 1105 98-99 1.9488577877310487 1105 100-101 1.7176513019835529 1106 1 0.019847112788063725 1106 2 0.047611887535303765 1106 3 0.10363505306525411 1106 4 0.16512663065221034 1106 5 0.1285555982735005 1106 6 0.0723515569258808 1106 7 0.0882088182776215 1106 8 0.06453744751020452 1106 9 -0.0775887830494355 1106 10-11 0.15653963208911392 1106 12-13 0.021441280439965738 1106 14-15 0.02553976861852192 1106 16-17 -0.015373080110371973 1106 18-19 0.12558916156271493 1106 20-21 0.10554115857334523 1106 22-23 0.1209138671276051 1106 24-25 0.11597856236898707 1106 26-27 -0.03790521344103581 1106 28-29 0.2189129486472794 1106 30-31 0.32168973100974796 1106 32-33 0.15363302678994728 1106 34-35 0.0768330353654747 1106 36-37 0.002654582898991009 1106 38-39 0.16809115018742915 1106 40-41 0.20388928634211112 1106 42-43 0.19108966281320505 1106 44-45 0.24437442114673757 1106 46-47 -0.3071904449466487 1106 48-49 0.02199246826001655 1106 50-51 0.2463139973303825 1106 52-53 0.019730944095989855 1106 54-55 0.021193294408881513 1106 56-57 0.24263079949799504 1106 58-59 0.42433566666629474 1106 60-61 0.5092689462247364 1106 62-63 0.39562105057576247 1106 64-65 0.41154112553557454 1106 66-67 0.620680207044046 1106 68-69 0.651005503102855 1106 70-71 0.46921597739182985 1106 72-73 0.62222232310965 1106 74-75 0.6018053011146023 1106 76-77 0.778533187848037 1106 78-79 0.9421320509820639 1106 80-81 1.0504725007458013 1106 82-83 1.3359418682425428 1106 84-85 1.4146310941092999 1106 86-87 1.4101748462823203 1106 88-89 1.5182935506032145 1106 90-91 1.5258803088357205 1106 92-93 1.6716666968847633 1106 94-95 1.5141871705817778 1106 96-97 1.7098352790196252 1106 98-99 2.247742563888549 1106 100-101 2.0162839960707917 1107 1 0.10918593257671105 1107 2 0.03726451594226177 1107 3 0.07853036360070575 1107 4 0.05982825268947778 1107 5 0.1516631348758466 1107 6 0.02125582451527208 1107 7 0.043202219983825785 1107 8 0.009693676403458085 1107 9 0.11279433363126401 1107 10-11 0.024388276581355228 1107 12-13 0.046333993221949754 1107 14-15 0.13078775016408173 1107 16-17 0.25713229723928777 1107 18-19 0.1189664488846347 1107 20-21 0.09399518353256298 1107 22-23 0.11155987645874177 1107 24-25 0.2292889315617117 1107 26-27 0.29566867186295553 1107 28-29 0.14528430270029702 1107 30-31 0.0968261130665482 1107 32-33 0.19030187556952427 1107 34-35 0.21209753862305547 1107 36-37 0.2980321403872779 1107 38-39 0.2433937884658448 1107 40-41 0.1973969808878735 1107 42-43 0.3140366582515526 1107 44-45 0.399425492979816 1107 46-47 0.23105368153276373 1107 48-49 0.2851558046067524 1107 50-51 0.2915149075831991 1107 52-53 0.51495731233274 1107 54-55 0.3659489497181738 1107 56-57 0.5376150571120135 1107 58-59 0.7098786490950886 1107 60-61 0.8547478160980724 1107 62-63 0.9781199854968428 1107 64-65 1.2110532674352577 1107 66-67 1.2010775594137186 1107 68-69 1.35665561688446 1107 70-71 1.2536892049446458 1107 72-73 1.466044345824404 1107 74-75 1.4965614759745804 1107 76-77 1.365498700073065 1107 78-79 1.7031657490399859 1107 80-81 1.747023723248578 1107 82-83 1.778928972994354 1107 84-85 1.9040348577345156 1107 86-87 2.1383485216878277 1107 88-89 2.2113882023483846 1107 90-91 2.3177670052206842 1107 92-93 2.4049558684092247 1107 94-95 1.8429562510809596 1107 96-97 2.171850927886485 1107 98-99 2.3162219429215636 1107 100-101 2.3242627360564523 1108 1 -0.013923178240290213 1108 2 -0.007127529300884561 1108 3 -0.05812059659362134 1108 4 0.036472515707771436 1108 5 -0.08596502834389952 1108 6 -0.023885002213901885 1108 7 -0.023758909819143526 1108 8 0.07643356021277015 1108 9 0.1442591017016781 1108 10-11 -0.05599444576754564 1108 12-13 -0.056092935803924604 1108 14-15 0.03696449980132144 1108 16-17 0.05445867560847972 1108 18-19 0.0282944590260783 1108 20-21 0.09842442202112522 1108 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2316 58-59 -0.5022875145973345 2316 60-61 -0.39870423900720553 2316 62-63 -0.5187765676940153 2316 64-65 -0.6106607936546702 2316 66-67 -0.46523004248021493 2316 68-69 -0.09431750890730584 2316 70-71 -0.41883716487151545 2316 72-73 -0.2946391603775247 2316 74-75 -0.22059632608112523 2316 76-77 -0.011574252105745586 2316 78-79 -0.18668182743230588 2316 80-81 -0.4281059547841011 2316 82-83 -0.05944491374534522 2316 84-85 0.14112341310315557 2316 86-87 0.41032342756181706 2316 88-89 0.3972751136044472 2316 90-91 0.6209739888710075 2316 92-93 0.8215924062450135 2316 94-95 0.9771424430023217 2316 96-97 0.5793458467477706 2316 98-99 0.6998929318348655 2316 100-101 0.2601235947352212 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 33.0 5 81.0 6 192.0 7 449.0 8 946.0 9 1756.0 10 2935.0 11 4621.0 12 6449.0 13 9079.0 14 12305.0 15 16551.0 16 21351.0 17 27062.0 18 25730.0 19 21067.0 20 20883.0 21 22559.0 22 24755.0 23 27548.0 24 31172.0 25 35018.0 26 40497.0 27 47209.0 28 55524.0 29 65033.0 30 74757.0 31 86501.0 32 100701.0 33 118775.0 34 142716.0 35 168078.0 36 184501.0 37 149790.0 38 41663.0 39 574.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.199459114413166 19.248967268615917 15.392513468630273 22.159060148340643 2 16.206653670609754 21.196308649847325 38.6405881082932 23.95644957124972 3 19.240657859872144 25.30976826621032 30.053013219003187 25.396560654914346 4 13.304310720282766 17.559268312945754 35.610393762634715 33.52602720413676 5 13.794781067539235 37.71821339581803 32.963762273667626 15.52324326297511 6 31.852202437446348 36.075880056089964 16.8387589717511 15.23315853471259 7 28.81319876440956 33.484740155934134 19.622047283740205 18.080013795916106 8 27.367648576729714 34.138661544387354 19.113952253039255 19.379737625843678 9 27.353688286251593 16.50262954505975 19.55291440195158 36.59076776673707 10-11 25.580787928069903 26.935991229019763 26.013200526661617 21.47002031624872 12-13 26.675775831468286 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22.131807621029882 38-39 21.844407649478327 26.1155856405634 29.833223546414374 22.206783163543903 40-41 22.163616707554844 25.878990924870138 29.83465859289065 22.12273377468437 42-43 22.183891442700677 25.91679954984937 29.466487789695567 22.43282121775439 44-45 22.055978359381292 26.22678844759526 29.336400094658828 22.380833098364615 46-47 22.21898443270099 26.59893742461256 28.711374805640844 22.470703337045602 48-49 22.418206329505892 26.54451952197654 28.471384802841758 22.56588934567581 50-51 22.497255921796288 26.792084467258615 28.141277229941164 22.56938238100393 52-53 22.939581188211037 26.7946019701978 27.823096327214962 22.442720514376198 54-55 22.21752987646613 26.86008851540334 28.054863941553656 22.867517666576877 56-57 22.295831234102355 27.11779494365669 28.018840997925892 22.56753282431506 58-59 22.46424516450623 26.47437559633351 28.00124112894902 23.06013811021124 60-61 22.23593911670892 26.76665768757285 28.371943436743962 22.625459758974266 62-63 22.108081436185074 26.972904115865553 28.223284888185106 22.69572955976426 64-65 21.907942954552393 26.728653980320022 28.072499163715676 23.290903901411912 66-67 21.89963335451198 27.034243637515974 27.890515522248748 23.175607485723297 68-69 22.132640004732963 26.72229155927815 28.002992088023028 23.142076347965865 70-71 21.91693614471632 27.020896559450275 27.719378629142398 23.342788666691003 72-73 22.178616822453435 26.92447244756574 27.630917686494904 23.26599304348592 74-75 21.965433425893526 27.17112600353961 27.34146654616219 23.521974024404674 76-77 21.940950709809954 26.922459650721642 27.85620149086524 23.280388148603162 78-79 22.076801462165708 26.98240768946098 27.73504666191698 23.205744186456336 80-81 21.866246746515692 27.378737199657493 27.592788223857973 23.16222782996884 82-83 21.965975498078805 27.1572295772742 27.480198004223165 23.396596920423825 84-85 21.819981954576768 26.872322808514898 27.51668981352538 23.791005423382956 86-87 22.133744882516197 26.851286841241485 27.405103306376823 23.609864969865498 88-89 21.8415408124989 27.035464063904296 27.181951266178107 23.941043857418702 90-91 21.65149272730305 26.896375279407447 27.62111170098439 23.831020292305112 92-93 21.82892647053733 26.56916109692035 27.51574786176183 24.08616457078049 94-95 21.53763560306368 26.481827230533312 27.511140747743134 24.469396418659876 96-97 21.822313383800875 26.385507742580288 27.35345615391006 24.438722719708775 98-99 21.577895317192784 26.461536331343666 27.070772042626363 24.88979630883719 100-101 21.634665008471398 26.168939552236925 27.47332076260199 24.723074676689684 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1355.0 1 940.0 2 619.5 3 747.0 4 691.5 5 665.5 6 1164.5 7 2492.0 8 3802.0 9 4115.0 10 3406.5 11 2693.0 12 2648.0 13 2788.5 14 3017.0 15 3239.5 16 3315.5 17 3462.5 18 3711.5 19 3984.0 20 4277.5 21 4716.0 22 5271.5 23 5887.0 24 6661.5 25 7753.5 26 9334.5 27 11114.0 28 12571.5 29 14162.5 30 16202.5 31 18707.5 32 22142.5 33 24195.0 34 25735.5 35 27820.5 36 29958.5 37 32485.5 38 34706.5 39 35988.0 40 37715.0 41 42295.0 42 46942.5 43 50904.5 44 53921.5 45 56277.0 46 57536.5 47 58946.0 48 62890.5 49 64387.5 50 63477.0 51 61730.0 52 58805.5 53 57857.0 54 58058.0 55 54070.5 56 47717.0 57 42730.5 58 38888.0 59 34778.5 60 29194.0 61 23925.5 62 19701.5 63 15332.5 64 11755.0 65 9408.0 66 7206.5 67 5730.5 68 4321.0 69 2936.0 70 2304.5 71 1967.5 72 1713.5 73 1517.5 74 1268.0 75 1076.5 76 767.0 77 416.5 78 231.0 79 142.5 80 79.5 81 33.5 82 21.0 83 13.0 84 5.0 85 4.0 86 2.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023286902187458304 2 0.01189520138764762 3 0.0016993144839496598 4 0.0 5 4.4056301435731925E-4 6 0.0 7 0.0 8 0.0 9 5.035005878369363E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017307832706894685 26-27 0.009409167235202746 28-29 1.573439336990426E-4 30-31 0.0 32-33 3.776254408777022E-4 34-35 0.010038542969998917 36-37 0.0014160954032913834 38-39 0.01211548289482628 40-41 0.012178420468305896 42-43 0.0046888492242314695 44-45 7.552508817554044E-4 46-47 3.146878673980852E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.146878673980852E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.573439336990426E-4 66-67 7.237820950155959E-4 68-69 0.0011958138961127237 70-71 1.2587514695923407E-4 72-73 3.461566541378937E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.573439336990426E-4 82-83 0.0013216890430719577 84-85 0.0067657891490588325 86-87 0.005192349812068405 88-89 0.0 90-91 0.0014160954032913834 92-93 5.979069480563619E-4 94-95 9.440636021942555E-5 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1588876.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.76405476985501 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48237337757789 33.6285135987041 2 13.64072283142916 12.485095735158717 3 5.0349975908472935 6.9126571654087074 4 2.392927368388853 4.3804023658893 5 1.3457071189627492 3.0792507148197523 6 0.8518430887112766 2.339027626026319 7 0.5818876773901507 1.8640677676590605 8 0.3844835331359868 1.407642037483413 9 0.30358118080110563 1.250379520675115 >10 1.7154659213841148 14.651802300480465 >50 0.15791028459027714 5.039474525824195 >100 0.10049716787468246 8.614933193557322 >500 0.005805819528616242 1.8011150678484622 >1k 0.0013823379830038673 0.9358257594985179 >5k 2.7646759660077347E-4 0.9488576060784336 >10k+ 1.3823379830038674E-4 0.6609550148881576 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10448 0.6575717677150388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8694 0.5471792638317905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6305 0.3968214007889854 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2140 0.13468640724638045 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2031 0.1278262117371022 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1839 0.11574219762901573 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 6.293757347961704E-5 12-13 0.0 0.0 0.0 0.0 6.293757347961704E-5 14-15 0.0 0.0 0.0 0.0 6.293757347961704E-5 16-17 0.0 0.0 0.0 6.293757347961704E-5 6.293757347961704E-5 18-19 0.0 0.0 0.0 9.440636021942555E-5 6.293757347961704E-5 20-21 0.0 0.0 0.0 1.888127204388511E-4 6.293757347961704E-5 22-23 0.0 0.0 0.0 8.811260287146385E-4 6.293757347961704E-5 24-25 0.0 0.0 0.0 0.001762252057429277 6.293757347961704E-5 26-27 0.0 0.0 0.0 0.0030210035270216177 6.293757347961704E-5 28-29 0.0 0.0 0.0 0.00723782095015596 6.293757347961704E-5 30-31 0.0 0.0 0.0 0.01951064777868128 6.293757347961704E-5 32-33 0.0 0.0 0.0 0.043363988127456135 6.293757347961704E-5 34-35 0.0 0.0 0.0 0.08005659346607287 6.293757347961704E-5 36-37 0.0 0.0 0.0 0.13644865930380973 6.293757347961704E-5 38-39 0.0 0.0 0.0 0.22629204544596304 6.293757347961704E-5 40-41 0.0 0.0 0.0 0.32636278727855417 6.293757347961704E-5 42-43 0.0 0.0 0.0 0.42706290484594145 6.293757347961704E-5 44-45 0.0 0.0 0.0 0.5503261425057714 6.293757347961704E-5 46-47 0.0 0.0 0.0 0.6947993424282323 6.293757347961704E-5 48-49 0.0 0.0 0.0 0.8518285882598768 6.293757347961704E-5 50-51 0.0 0.0 0.0 1.0193054712891376 6.293757347961704E-5 52-53 0.0 0.0 0.0 1.1830690374831012 6.293757347961704E-5 54-55 0.0 0.0 0.0 1.3622837779663106 6.293757347961704E-5 56-57 0.0 0.0 0.0 1.5774988104798613 6.293757347961704E-5 58-59 0.0 0.0 0.0 1.805301357689335 6.293757347961704E-5 60-61 0.0 0.0 0.0 2.0557299625647314 6.293757347961704E-5 62-63 0.0 0.0 0.0 2.3036410645009426 6.293757347961704E-5 64-65 0.0 0.0 0.0 2.5572480168370597 6.293757347961704E-5 66-67 0.0 0.0 0.0 2.8482398878200694 6.293757347961704E-5 68-69 0.0 0.0 0.0 3.1717075467185607 6.293757347961704E-5 70-71 0.0 0.0 0.0 3.5183677014442916 6.293757347961704E-5 72-73 0.0 0.0 0.0 3.8772692142118075 6.293757347961704E-5 74-75 0.0 0.0 0.0 4.242338609180326 6.293757347961704E-5 76-77 0.0 0.0 0.0 4.636233412802509 6.293757347961704E-5 78-79 0.0 3.146878673980852E-5 0.0 5.063894224596507 6.293757347961704E-5 80-81 0.0 6.293757347961704E-5 0.0 5.524345512173385 6.293757347961704E-5 82-83 0.0 6.293757347961704E-5 0.0 5.998737472275999 6.293757347961704E-5 84-85 0.0 6.293757347961704E-5 0.0 6.463342639702532 6.293757347961704E-5 86-87 0.0 6.293757347961704E-5 0.0 6.93852131947364 6.293757347961704E-5 88-89 0.0 6.293757347961704E-5 0.0 7.445042910837598 6.293757347961704E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4865 0.0 58.41182 1 GTATCAA 8755 0.0 41.685734 1 TATCAAC 11640 0.0 31.305122 2 ATCAACG 11755 0.0 30.91122 3 TCAACGC 12010 0.0 30.294455 4 CAACGCA 12285 0.0 29.61631 5 TGGTATC 1995 0.0 29.52921 2 AACGCAG 12730 0.0 28.655642 6 GTGGTAT 2195 0.0 26.628885 1 GTAAGGT 615 0.0 24.714523 4 ACGCAGA 14805 0.0 24.511072 7 CGCAGAG 14925 0.0 24.218525 8 TATCACG 100 0.0016521636 23.754343 2 TAAGGTG 740 0.0 23.749111 5 GTACATG 9500 0.0 22.95998 1 GAGTACT 8635 0.0 22.80025 12-13 GCAGAGT 15515 0.0 22.654646 9 AGTACTT 8675 0.0 22.147585 12-13 TACATGG 9860 0.0 21.634281 2 GTACTTT 9040 0.0 21.594877 14-15 >>END_MODULE