FastQCFastQC Report
Thu 26 May 2016
SRR938647_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938647_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498584
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74330.21245738275828163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59310.16952572812314926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39650.11333156499886811No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52900.046.030611
GTATCAA87700.042.081821
ATCAACG112250.033.0876583
TCAACGC113300.032.6970754
TATCAAC115050.032.241062
CAACGCA115450.032.046975
AACGCAG119400.031.1211556
ACGCAGA135650.027.1151317
CGCAGAG138000.026.5845178
GTACATG113300.024.80791
TACATGG114100.023.9653362
GCAGAGT149100.023.521739
ACATGGG116600.023.0844353
GAGTACT81200.021.00893812-13
CATGGGG75550.020.33154
GTGTAGC21250.020.1426221
AGTACTT86050.020.0733212-13
GTACTTT85850.019.86546314-15
AGAGTAC143300.019.8307610-11
TAGCCCT21450.019.7316784
Produced by FastQC (version 0.11.5)