##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938647_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3498584 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.029605406072857 31.0 31.0 34.0 28.0 34.0 2 31.06986026346659 31.0 31.0 34.0 27.0 34.0 3 31.121021247453257 31.0 31.0 34.0 27.0 34.0 4 34.771589591674804 37.0 35.0 37.0 32.0 37.0 5 34.735630758043825 37.0 35.0 37.0 32.0 37.0 6 34.74837534271008 37.0 35.0 37.0 32.0 37.0 7 34.650540618718885 37.0 35.0 37.0 30.0 37.0 8 34.62322899778882 37.0 35.0 37.0 30.0 37.0 9 36.10397377910606 39.0 35.0 39.0 30.0 39.0 10-11 35.924784284156104 38.0 35.0 39.0 30.0 39.0 12-13 35.776199742524405 38.0 35.0 39.0 30.0 39.0 14-15 36.73411443029523 39.0 36.0 41.0 30.0 41.0 16-17 36.550467417675264 39.0 36.0 40.5 30.0 41.0 18-19 36.67986634021078 39.0 36.0 41.0 30.0 41.0 20-21 36.65821701008179 39.0 36.0 41.0 30.0 41.0 22-23 36.56173440454767 39.0 36.0 41.0 30.0 41.0 24-25 36.42475684448337 39.0 36.0 41.0 29.0 41.0 26-27 36.21068037811869 39.0 35.0 41.0 28.5 41.0 28-29 35.993960842443684 39.0 35.0 40.0 27.0 41.0 30-31 35.75902922439478 38.5 35.0 40.0 27.0 41.0 32-33 35.50476578524341 38.0 34.0 40.0 27.0 41.0 34-35 35.25400805011399 38.0 34.0 40.0 25.5 41.0 36-37 34.98339571095049 38.0 34.0 40.0 25.0 41.0 38-39 34.87683617143393 38.0 34.0 40.0 25.0 41.0 40-41 34.77999785055897 38.0 33.5 40.0 24.5 41.0 42-43 34.504913702229246 38.0 33.0 40.0 24.0 41.0 44-45 34.17467981331876 38.0 33.0 40.0 23.5 41.0 46-47 33.8003169282201 38.0 33.0 40.0 21.5 41.0 48-49 33.454782849289884 38.0 32.0 40.0 19.5 41.0 50-51 32.77235961749096 36.5 31.5 39.5 18.0 40.5 52-53 32.803058037194475 37.0 31.5 39.0 18.0 40.0 54-55 32.9060641390917 37.0 31.5 40.0 17.0 41.0 56-57 32.58311691244229 37.0 31.0 40.0 13.5 41.0 58-59 32.2001987089634 36.5 31.0 39.0 10.5 41.0 60-61 31.710826580124987 36.0 30.5 39.0 8.0 40.5 62-63 31.121191745003124 35.0 29.0 39.0 6.5 40.0 64-65 30.599583431468275 35.0 29.0 38.5 2.0 40.0 66-67 30.04477968801092 34.5 28.0 38.0 2.0 40.0 68-69 29.438537419710375 34.0 27.5 37.5 2.0 40.0 70-71 28.80139336371515 34.0 26.0 37.0 2.0 39.0 72-73 28.14168503600314 33.5 26.0 36.0 2.0 39.0 74-75 27.442500880356167 33.0 25.0 36.0 2.0 38.5 76-77 26.676228725678733 32.0 23.0 35.0 2.0 37.5 78-79 25.88240370961509 32.0 20.5 35.0 2.0 37.0 80-81 25.17445572265808 31.0 19.0 35.0 2.0 36.0 82-83 24.430092717510856 31.0 14.5 34.0 2.0 36.0 84-85 23.703435875771454 30.5 7.5 34.0 2.0 35.0 86-87 22.92134489267658 30.0 2.0 34.0 2.0 35.0 88-89 22.10474294743245 29.0 2.0 34.0 2.0 35.0 90-91 21.184030453463457 29.0 2.0 34.0 2.0 35.0 92-93 20.226204944629025 27.0 2.0 33.5 2.0 35.0 94-95 19.203789304472895 25.5 2.0 33.0 2.0 35.0 96-97 17.942058558548258 24.0 2.0 32.5 2.0 34.0 98-99 16.427298158340633 10.5 2.0 32.0 2.0 34.0 100-101 12.986577569668185 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28448184758351047 1101 2 0.3044134415386175 1101 3 0.34608853130768935 1101 4 0.27112910173826776 1101 5 0.24340971547272972 1101 6 0.3506155333765193 1101 7 0.21469226348622783 1101 8 0.4453747644300421 1101 9 0.5458013923049805 1101 10-11 0.5994971587186768 1101 12-13 0.6558325289722902 1101 14-15 0.7664761611794404 1101 16-17 0.5767571361740806 1101 18-19 0.6396356501604643 1101 20-21 0.6682836234156468 1101 22-23 0.6381492192214111 1101 24-25 0.6404630649677898 1101 26-27 0.6628924933738602 1101 28-29 0.6743107223939546 1101 30-31 0.6308444088469543 1101 32-33 0.7667868366873947 1101 34-35 0.6874652161730026 1101 36-37 0.6315567196295149 1101 38-39 0.6054975878887774 1101 40-41 0.6401756390807591 1101 42-43 0.8305280659388714 1101 44-45 0.8948983151724548 1101 46-47 0.892144887724065 1101 48-49 0.7464298774075289 1101 50-51 0.7476235729990179 1101 52-53 0.7316722940627471 1101 54-55 0.7604058445856836 1101 56-57 0.6969872387139233 1101 58-59 0.8731298691385305 1101 60-61 0.797232169112366 1101 62-63 0.6618880268194296 1101 64-65 0.8625820320063262 1101 66-67 0.7496796275543751 1101 68-69 0.8705168939515637 1101 70-71 0.9406455537115157 1101 72-73 0.8788254673499729 1101 74-75 0.9406539797470934 1101 76-77 0.8853961756828284 1101 78-79 1.1057742524229717 1101 80-81 1.2555781283423464 1101 82-83 1.1906354285528096 1101 84-85 1.06258714880072 1101 86-87 1.2526976784573023 1101 88-89 1.1498816431180785 1101 90-91 0.9164483582067966 1101 92-93 0.7922146261598435 1101 94-95 0.8912104716012124 1101 96-97 0.6916774818971021 1101 98-99 0.967709657321759 1101 100-101 0.9258892294138992 1102 1 0.45057534385897924 1102 2 0.38394613172888725 1102 3 0.3924124933488571 1102 4 0.37369756983123636 1102 5 0.34647199552556884 1102 6 0.39276414524314873 1102 7 0.4679798521841576 1102 8 0.4219133979242784 1102 9 0.6987027520287796 1102 10-11 0.7591182163220012 1102 12-13 0.6923146662606925 1102 14-15 0.7879050065394964 1102 16-17 0.871192794786829 1102 18-19 0.7727869536201766 1102 20-21 0.7356495458489221 1102 22-23 0.7541023832814346 1102 24-25 0.7707707866205311 1102 26-27 0.6811848809188632 1102 28-29 0.45946028393777993 1102 30-31 0.7153195582873906 1102 32-33 0.7420088401328755 1102 34-35 0.581660478282906 1102 36-37 0.8476107048928654 1102 38-39 0.7978271026652877 1102 40-41 1.0204017651950608 1102 42-43 0.9526869314934387 1102 44-45 1.002137560153848 1102 46-47 1.0122142615931082 1102 48-49 1.1193530262182136 1102 50-51 1.0289585809668225 1102 52-53 0.9274652038797484 1102 54-55 0.923504981398743 1102 56-57 0.9148780066606506 1102 58-59 0.8719654296549706 1102 60-61 0.8071605850487735 1102 62-63 0.8124305841659059 1102 64-65 0.7818344917308266 1102 66-67 0.7807420448613058 1102 68-69 0.9546881164111731 1102 70-71 1.2121841014219434 1102 72-73 1.1269942353834956 1102 74-75 1.1065226215270378 1102 76-77 1.1472008348536704 1102 78-79 1.1398259830860447 1102 80-81 1.2509134067814465 1102 82-83 1.2907795453479665 1102 84-85 1.3236535121627604 1102 86-87 1.5307830949074095 1102 88-89 1.3249694201367 1102 90-91 1.1272055970985555 1102 92-93 1.3849619080747004 1102 94-95 1.4321781432167633 1102 96-97 1.5099104263102028 1102 98-99 1.2898593389629163 1102 100-101 1.4245520112515493 1103 1 0.3110530990719518 1103 2 0.35624725736871454 1103 3 0.19884774074803602 1103 4 0.259823832495627 1103 5 0.1771340733162674 1103 6 0.43316185621568337 1103 7 0.3074257520165631 1103 8 0.3677056490529367 1103 9 0.4911068537062917 1103 10-11 0.5112171835417954 1103 12-13 0.49752164696401024 1103 14-15 0.6204430563364198 1103 16-17 0.6161582272257888 1103 18-19 0.6308654577497848 1103 20-21 0.48490784848070234 1103 22-23 0.5464829397597057 1103 24-25 0.6625404419074883 1103 26-27 0.6267558354855893 1103 28-29 0.588920707241769 1103 30-31 0.6354917543797427 1103 32-33 0.580246054948141 1103 34-35 0.658688668350699 1103 36-37 0.6489697205808582 1103 38-39 0.8324396307874125 1103 40-41 0.7432759498422712 1103 42-43 0.6602955697032868 1103 44-45 0.6923474285649718 1103 46-47 0.40884644877696275 1103 48-49 0.5863916346827764 1103 50-51 0.6919780852625266 1103 52-53 0.6395179394638575 1103 54-55 0.5984604861625229 1103 56-57 0.649760997872967 1103 58-59 0.5727837079016567 1103 60-61 0.7855772340997937 1103 62-63 0.8039633719174937 1103 64-65 0.8330620506551654 1103 66-67 0.9123757042099321 1103 68-69 0.9433418783583569 1103 70-71 1.0878340716011685 1103 72-73 0.9901374109561267 1103 74-75 0.9829775166636097 1103 76-77 0.9565081718963562 1103 78-79 0.8747706919748985 1103 80-81 0.979342465426452 1103 82-83 0.9232884821430289 1103 84-85 1.013051755379479 1103 86-87 1.13803841357446 1103 88-89 1.1641314092200297 1103 90-91 0.9282673959329024 1103 92-93 0.9376040191589681 1103 94-95 0.7988672069199758 1103 96-97 1.1133835555285252 1103 98-99 1.1137871594796103 1103 100-101 0.9752039160062633 1104 1 0.3675819898443713 1104 2 0.2697748867472036 1104 3 0.35109684607157376 1104 4 0.277057591335371 1104 5 0.43413006510864705 1104 6 0.49221621030778806 1104 7 0.3204709766987861 1104 8 0.540935416808054 1104 9 0.5123666566206211 1104 10-11 0.5322117584063832 1104 12-13 0.6082208252029631 1104 14-15 0.5741799358419044 1104 16-17 0.6885222314095074 1104 18-19 0.7147397706896399 1104 20-21 0.738390683392204 1104 22-23 0.7860909765798567 1104 24-25 0.5874835792966877 1104 26-27 0.7307963984325667 1104 28-29 0.7960168150424352 1104 30-31 0.8800101665930029 1104 32-33 0.8285326853140589 1104 34-35 0.7257423146923685 1104 36-37 0.9504123685377976 1104 38-39 0.9387681646880921 1104 40-41 1.0843109742139987 1104 42-43 0.9940387847167287 1104 44-45 0.9239872085630267 1104 46-47 0.7673838489641795 1104 48-49 0.8754049506648087 1104 50-51 0.9594747534131827 1104 52-53 0.8959791052258552 1104 54-55 0.9738989953490105 1104 56-57 0.9683236972844966 1104 58-59 0.9227226002151454 1104 60-61 0.8926025490202001 1104 62-63 0.9190933925221607 1104 64-65 1.0159713044921475 1104 66-67 1.107846904046692 1104 68-69 1.067656245121487 1104 70-71 1.1407486728066303 1104 72-73 1.198462807068065 1104 74-75 1.1291281604882037 1104 76-77 1.089744421418338 1104 78-79 1.2992515976956902 1104 80-81 1.2958550429793476 1104 82-83 1.2543236777447007 1104 84-85 1.2505428728260988 1104 86-87 1.330332852025851 1104 88-89 1.501445749585205 1104 90-91 1.5843152197429937 1104 92-93 1.5595217909722372 1104 94-95 1.599138891765552 1104 96-97 1.6568805097758847 1104 98-99 1.9597523577068081 1104 100-101 1.404958971570382 1105 1 0.3958793613883351 1105 2 0.2708992232701384 1105 3 0.16274467320886643 1105 4 0.1679782040651574 1105 5 0.17051215258532437 1105 6 0.2775985578545317 1105 7 0.3224422640893181 1105 8 0.28530041127631733 1105 9 0.42686984126011396 1105 10-11 0.44214081156137297 1105 12-13 0.44048979615429573 1105 14-15 0.5409819162746103 1105 16-17 0.4485095439577407 1105 18-19 0.46188887657480393 1105 20-21 0.5505883225208663 1105 22-23 0.5777926736925423 1105 24-25 0.6614828433000781 1105 26-27 0.6488034262082891 1105 28-29 0.48940072166467274 1105 30-31 0.5334168743161101 1105 32-33 0.643665358873001 1105 34-35 0.6095911062239381 1105 36-37 0.587755757017085 1105 38-39 0.5843244380840957 1105 40-41 0.763925540961985 1105 42-43 0.9035722270878424 1105 44-45 0.9825078486615269 1105 46-47 0.8404497942936544 1105 48-49 0.81343228811388 1105 50-51 0.9588652194496561 1105 52-53 0.9244364358310619 1105 54-55 0.9130241915993622 1105 56-57 0.8389334178529211 1105 58-59 0.9196711058377574 1105 60-61 0.8445890674049004 1105 62-63 0.886368609694113 1105 64-65 1.0975710195078037 1105 66-67 1.1393697243964418 1105 68-69 1.1488668637133514 1105 70-71 1.0466440762237177 1105 72-73 0.9572664393743828 1105 74-75 1.180784808489335 1105 76-77 1.4226468403389347 1105 78-79 1.2708484413495356 1105 80-81 1.392069367611679 1105 82-83 1.397669565720907 1105 84-85 1.4325417794222766 1105 86-87 1.5387398816844318 1105 88-89 1.5220857220013038 1105 90-91 1.4357099316292405 1105 92-93 1.6419047842391628 1105 94-95 1.6066670964230418 1105 96-97 1.8348181121248857 1105 98-99 1.8005893092418184 1105 100-101 1.4202750726981304 1106 1 0.13820623470535054 1106 2 0.031069847085472446 1106 3 -0.026136339839258227 1106 4 -0.05926317515971391 1106 5 0.13389910488859869 1106 6 -0.13570257771244343 1106 7 0.11953337706459877 1106 8 0.14100725109286572 1106 9 -0.08926645652168474 1106 10-11 -0.03527013142133484 1106 12-13 0.11311363346827363 1106 14-15 0.4579235603155638 1106 16-17 0.16397156089107057 1106 18-19 -0.006413090596133486 1106 20-21 0.18779396962813166 1106 22-23 -0.0014673218937204524 1106 24-25 -0.12894382943223803 1106 26-27 -0.019192305800252996 1106 28-29 0.2440925089125372 1106 30-31 -0.16748802481029657 1106 32-33 0.051923000272317665 1106 34-35 -0.22171937354750781 1106 36-37 -0.13614582139449993 1106 38-39 -0.1885737748308074 1106 40-41 0.04170423218673136 1106 42-43 -0.4762963224767063 1106 44-45 -0.2242110486274953 1106 46-47 -0.06339464511811599 1106 48-49 0.04876711962877067 1106 50-51 -0.1990518691813108 1106 52-53 -0.1907256748880215 1106 54-55 -0.10442449537936938 1106 56-57 -0.8323338267750593 1106 58-59 -0.5737678983460484 1106 60-61 0.06975461773379266 1106 62-63 0.037307248063058296 1106 64-65 0.12543433769269896 1106 66-67 -0.22289753287290281 1106 68-69 -0.08693516150048097 1106 70-71 -0.18084971731723343 1106 72-73 0.15972329408379338 1106 74-75 0.4112217281687869 1106 76-77 0.26936219473896017 1106 78-79 0.5081896155280532 1106 80-81 0.6150500675421213 1106 82-83 0.39404616027915296 1106 84-85 0.48184523571374527 1106 86-87 0.17755730367580824 1106 88-89 0.15901962092998545 1106 90-91 0.33402951327089525 1106 92-93 0.3398955993324968 1106 94-95 0.16453751938929528 1106 96-97 0.24945679569706058 1106 98-99 0.5490663751084028 1106 100-101 0.16260450733881093 1107 1 0.25088979639779296 1107 2 0.10880484977205285 1107 3 0.24297352851843002 1107 4 0.20943047957449323 1107 5 0.30192648517115117 1107 6 0.30403404579156046 1107 7 0.22461405043249982 1107 8 0.16360658160275676 1107 9 0.2715983585606594 1107 10-11 0.3943117905082332 1107 12-13 0.4079939366080154 1107 14-15 0.5378615646825224 1107 16-17 0.6045705505097274 1107 18-19 0.5158549718870731 1107 20-21 0.6105665416334887 1107 22-23 0.6157521762611609 1107 24-25 0.6072687675760307 1107 26-27 0.5466654015731436 1107 28-29 0.6457479619683895 1107 30-31 0.7394095814109107 1107 32-33 0.7498123021802954 1107 34-35 0.8190970847192887 1107 36-37 0.7346583809453548 1107 38-39 0.7113545302934625 1107 40-41 0.6568181487862361 1107 42-43 0.8734566581038976 1107 44-45 0.72013097268011 1107 46-47 0.6580214189694331 1107 48-49 0.7171575284828151 1107 50-51 0.6041975751279622 1107 52-53 0.5729766241002068 1107 54-55 0.6841345089014084 1107 56-57 0.7098950252958858 1107 58-59 0.6933724598178586 1107 60-61 0.6317251433145721 1107 62-63 0.9044440183150435 1107 64-65 0.8163006386299614 1107 66-67 0.8774290383949079 1107 68-69 1.028498261427682 1107 70-71 1.1794775842054968 1107 72-73 1.102984189613938 1107 74-75 0.948207833049235 1107 76-77 1.1962232438602456 1107 78-79 1.269697823213189 1107 80-81 1.4114584805857113 1107 82-83 1.1593055575986568 1107 84-85 1.293439466161285 1107 86-87 1.3908581525456718 1107 88-89 1.5193280324528686 1107 90-91 1.4820313948257642 1107 92-93 1.5165074747031682 1107 94-95 1.653311988473373 1107 96-97 1.8109143211561864 1107 98-99 1.8521103263166019 1107 100-101 1.5667516186974577 1108 1 0.42213784225500817 1108 2 0.2849246821500273 1108 3 0.3031285571791926 1108 4 0.2823136724990434 1108 5 0.20365119711602375 1108 6 0.2775870754366281 1108 7 0.3958838089873069 1108 8 0.297748531982009 1108 9 0.3570459658213849 1108 10-11 0.43340776425035443 1108 12-13 0.44171058952331776 1108 14-15 0.4988922483429903 1108 16-17 0.513521067271931 1108 18-19 0.5744037132743713 1108 20-21 0.5831121925801597 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-1.6929352920856253 2316 64-65 -1.822522086562298 2316 66-67 -1.9119555879506756 2316 68-69 -2.0254452343256055 2316 70-71 -2.281127500566793 2316 72-73 -2.1459003268936137 2316 74-75 -1.9166450689957522 2316 76-77 -2.1700646367676697 2316 78-79 -2.4916272674099353 2316 80-81 -2.560207894618852 2316 82-83 -2.473601094106286 2316 84-85 -2.4775603783922584 2316 86-87 -2.495567398047829 2316 88-89 -2.7594616765600044 2316 90-91 -2.7807939160131703 2316 92-93 -2.792184390751448 2316 94-95 -3.034559119697928 2316 96-97 -3.1367648578706344 2316 98-99 -3.1145266746355063 2316 100-101 -2.8264530570563497 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13406.0 3 4959.0 4 5404.0 5 5980.0 6 7259.0 7 8761.0 8 10888.0 9 13585.0 10 16620.0 11 20457.0 12 24109.0 13 28141.0 14 31833.0 15 35485.0 16 40597.0 17 45968.0 18 45191.0 19 45096.0 20 48495.0 21 52693.0 22 57431.0 23 64404.0 24 70659.0 25 79114.0 26 88722.0 27 99424.0 28 112160.0 29 127252.0 30 146161.0 31 168961.0 32 196428.0 33 232475.0 34 279860.0 35 337436.0 36 392436.0 37 373491.0 38 160521.0 39 6722.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.689506884995474 18.086505225919094 14.268542836986311 23.955445052099115 2 15.750041632259249 19.240198392737273 40.26417456821195 24.745585406791523 3 19.597520739199734 22.98561490761434 28.829133031342735 28.58773132184319 4 13.218786995837151 15.994621232738137 36.13867123615782 34.6479205352669 5 14.724776788014745 36.37820017337076 33.6479829331721 15.249040105442399 6 33.589383177270626 34.81243149696319 17.184989026639617 14.413196299126566 7 30.13896294559703 31.350961132974714 19.869971509808604 18.640104411619653 8 26.690921255024847 35.09983931930856 19.108879752517428 19.10035967314917 9 26.612722236310454 16.826562547797945 19.5411854947557 37.0195297211359 10-11 25.717032313765348 25.916057620249877 27.003668934793513 21.363241131191266 12-13 26.316962747291118 23.04695889513058 27.353185353553698 23.282893004024608 14-15 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27.64474834470696 23.106153908836117 40-41 23.32869546718638 26.032968880547212 27.582250640351496 23.056085011914913 42-43 23.223948877038985 25.919898783399333 27.530672346995665 23.325479992566013 44-45 23.168653711144298 26.314199240322804 27.391856909929547 23.125290138603354 46-47 23.201881816987665 26.5026084811263 26.985426552664105 23.31008314922193 48-49 23.43633781574965 26.251964546966466 27.09831339104961 23.213384246234273 50-51 23.34283889727073 26.42301893890236 27.030642294461714 23.203499869365196 52-53 23.367768665915055 26.471851052298177 26.927679226858704 23.232701054928068 54-55 23.158254019822504 26.31252312799556 27.1075044262106 23.42171842597133 56-57 23.18074182090121 26.624245645896917 27.106363338930166 23.088649194271703 58-59 23.298985370478 26.294388076137665 26.99523174726059 23.41139480612374 60-61 23.284547104310853 26.339631919731083 27.312001955547043 23.06381902041102 62-63 23.17674718493827 26.38729183350883 27.07993544664436 23.356025534908536 64-65 23.040940553777936 26.279189527309065 27.083670701554862 23.596199217358134 66-67 23.033399843348903 26.420436679225435 27.067923115184296 23.47824036224137 68-69 23.02759201483283 26.166947669449254 27.19455169050474 23.61090862521318 70-71 22.99700816407521 26.241408907688253 27.08873835674327 23.672844571493272 72-73 23.274916968574217 26.20432199424666 26.88469864449312 23.636062392686 74-75 23.08158893772898 26.3827981731379 26.822473354448874 23.713139534684245 76-77 23.1501713792996 26.28128504884646 26.813191274393567 23.755352297460373 78-79 23.290087800237593 26.25366024886834 26.82738031943782 23.628871631456246 80-81 23.05580797202841 26.564190501562567 26.78263278114868 23.59736874526034 82-83 23.322308679910066 26.5581857158395 26.574850612635046 23.544654991615385 84-85 23.39131263732157 26.165135039454423 26.60807994275103 23.83547238047298 86-87 23.24147442502601 26.09963905067741 26.83158590295147 23.827300621345106 88-89 23.394456522873554 26.144492515114955 26.670429589382493 23.790621372628998 90-91 23.30272892084813 26.27933578117166 26.66009315853349 23.757842139446726 92-93 23.422664466089646 25.834569311385696 26.759969607448603 23.982796615076047 94-95 23.299885329299364 25.976044976079294 26.69764110072434 24.026428593897002 96-97 23.37424998763264 25.99335622652883 26.616599596981395 24.01579418885714 98-99 23.524980290661812 25.760172913258184 26.370243938351052 24.344602857728955 100-101 24.182789575198463 25.25987490306804 26.401562467373513 24.155773054359987 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 381.0 1 264.0 2 206.0 3 290.5 4 249.0 5 228.0 6 442.5 7 1021.0 8 1638.0 9 1922.0 10 1866.5 11 1762.0 12 1832.5 13 1963.0 14 2209.5 15 2481.5 16 2637.0 17 2774.0 18 3001.5 19 3308.0 20 3710.5 21 4308.0 22 5322.5 23 6601.0 24 8108.5 25 10007.5 26 12815.0 27 16626.5 28 20206.0 29 24324.5 30 29259.0 31 34766.5 32 41313.5 33 47622.5 34 53217.5 35 58588.5 36 65956.0 37 73686.5 38 79468.5 39 84635.0 40 90826.0 41 100382.5 42 109844.5 43 116713.5 44 123524.0 45 132121.5 46 137773.0 47 140997.0 48 145285.0 49 145902.5 50 143959.0 51 140554.5 52 136963.5 53 134972.0 54 132968.5 55 125710.5 56 113938.5 57 104335.5 58 94101.0 59 80990.5 60 67732.0 61 56069.5 62 45927.0 63 36953.0 64 29749.0 65 23531.5 66 18031.0 67 14444.0 68 11131.5 69 7884.0 70 5924.5 71 4523.0 72 3630.0 73 2922.0 74 2184.0 75 1720.5 76 1280.5 77 834.0 78 525.0 79 332.0 80 203.0 81 115.0 82 66.0 83 45.0 84 33.0 85 24.0 86 16.0 87 11.5 88 8.5 89 8.0 90 7.0 91 5.5 92 3.0 93 2.5 94 3.5 95 5.0 96 3.0 97 0.0 98 0.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007202914093244582 2 0.10569990601912088 3 0.1141604717794399 4 0.11727601795469252 5 0.15697779444483825 6 0.09435245802301731 7 0.025410280273390606 8 0.027554004705903874 9 0.02249481504517256 10-11 0.05123501393706711 12-13 0.0527356210398264 14-15 0.026853721391282875 16-17 0.030955380805491595 18-19 0.03617177692460721 20-21 0.05772335321947394 22-23 0.030355137964387876 24-25 0.04730485247745945 26-27 0.055865458711295765 28-29 0.052807078520910176 30-31 0.06665553835494589 32-33 0.04920562147428788 34-35 0.061224769792578945 36-37 0.052807078520910176 38-39 0.031198336241176426 40-41 0.03675772826949417 42-43 0.03241311341960062 44-45 0.007317246062978623 46-47 0.013591212902134121 48-49 0.009203723563590298 50-51 0.008060403866249888 52-53 0.009603885457659441 54-55 0.01110449256041873 56-57 0.013591212902134121 58-59 0.01877902602881623 60-61 0.02405258813279887 62-63 0.01121882453015277 64-65 0.00870352119600387 66-67 0.010404209245797729 68-69 0.011376030988537076 70-71 0.03046946993412192 72-73 0.02751113021725361 74-75 0.028640158418377262 76-77 0.02249481504517256 78-79 0.005373602577499925 80-81 0.005059189660731313 82-83 0.006073885892120927 84-85 0.005159230134248599 86-87 0.012790889113995833 88-89 0.037229347644647096 90-91 0.03465687832563117 92-93 0.02905461180866316 94-95 0.046132949787685534 96-97 0.042159913839427604 98-99 0.025052992867971725 100-101 0.07360120551628888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3498584.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.61862152751452 #Duplication Level Percentage of deduplicated Percentage of total 1 76.40070277486993 40.96500366521879 2 12.768424458261347 13.692506370603494 3 4.499751141498898 7.238113602720925 4 2.0982740962938227 4.5002625852066425 5 1.1160582651903188 2.992075286194707 6 0.7005156190098338 2.25364091098805 7 0.4441566040937067 1.6670545397692595 8 0.3283836932330915 1.4085984770618052 9 0.23888036266986 1.1527592180715567 >10 1.2489236797230483 12.075239650796531 >50 0.09110059890157186 3.406618815870001 >100 0.06054910340352735 6.24612991553507 >500 0.003370187249046053 1.2315748818490544 >1k 8.024255313236824E-4 0.7870984511802762 >5k 1.0699007084315765E-4 0.38332362893382943 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7433 0.21245738275828163 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5931 0.16952572812314926 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3965 0.11333156499886811 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.858299243351024E-5 0.0 0.0 0.0 0.0 3 2.858299243351024E-5 0.0 0.0 0.0 0.0 4 2.858299243351024E-5 0.0 0.0 0.0 0.0 5 5.716598486702048E-5 0.0 0.0 0.0 0.0 6 5.716598486702048E-5 0.0 0.0 0.0 0.0 7 8.574897730053072E-5 0.0 0.0 0.0 0.0 8 8.574897730053072E-5 0.0 0.0 0.0 0.0 9 8.574897730053072E-5 0.0 0.0 0.0 0.0 10-11 8.574897730053072E-5 0.0 0.0 0.0 0.0 12-13 8.574897730053072E-5 0.0 0.0 0.0 0.0 14-15 1.1433196973404097E-4 0.0 0.0 0.0 4.287448865026536E-5 16-17 1.1433196973404097E-4 0.0 0.0 1.429149621675512E-5 5.716598486702048E-5 18-19 1.2862346595079609E-4 0.0 0.0 5.716598486702048E-5 5.716598486702048E-5 20-21 1.429149621675512E-4 0.0 0.0 5.716598486702048E-5 5.716598486702048E-5 22-23 1.429149621675512E-4 0.0 0.0 2.000809470345717E-4 7.14574810837756E-5 24-25 1.429149621675512E-4 0.0 0.0 4.287448865026536E-4 1.1433196973404097E-4 26-27 1.429149621675512E-4 0.0 0.0 7.860322919215316E-4 1.1433196973404097E-4 28-29 2.000809470345717E-4 0.0 0.0 0.00234380537954784 1.1433196973404097E-4 30-31 2.5724693190159217E-4 0.0 0.0 0.007888905911648827 1.1433196973404097E-4 32-33 2.715384281183473E-4 0.0 0.0 0.02156586779108348 1.1433196973404097E-4 34-35 2.858299243351024E-4 0.0 0.0 0.04190266690752602 1.1433196973404097E-4 36-37 2.858299243351024E-4 0.0 0.0 0.07714549657804415 1.1433196973404097E-4 38-39 3.1441291676861266E-4 2.858299243351024E-5 0.0 0.13106731180386122 1.1433196973404097E-4 40-41 3.1441291676861266E-4 2.858299243351024E-5 0.0 0.19080576598989762 1.1433196973404097E-4 42-43 3.429959092021229E-4 2.858299243351024E-5 0.0 0.2522020337370776 1.1433196973404097E-4 44-45 4.8591087136967416E-4 2.858299243351024E-5 0.0 0.33143408876276803 1.1433196973404097E-4 46-47 5.144938638031843E-4 2.858299243351024E-5 0.0 0.42565792331983454 1.2862346595079609E-4 48-49 5.144938638031843E-4 2.858299243351024E-5 0.0 0.5284995300956044 1.429149621675512E-4 50-51 5.144938638031843E-4 2.858299243351024E-5 0.0 0.6368147799223914 1.429149621675512E-4 52-53 5.144938638031843E-4 2.858299243351024E-5 0.0 0.744872782817277 1.5720645838430633E-4 54-55 5.144938638031843E-4 2.858299243351024E-5 0.0 0.8658360067958923 1.7149795460106145E-4 56-57 5.144938638031843E-4 2.858299243351024E-5 0.0 1.009765665194833 1.7149795460106145E-4 58-59 5.144938638031843E-4 2.858299243351024E-5 0.0 1.1687442691100172 1.8578945081781657E-4 60-61 5.144938638031843E-4 2.858299243351024E-5 0.0 1.3394704829153739 2.000809470345717E-4 62-63 5.144938638031843E-4 2.858299243351024E-5 0.0 1.5092820409628582 2.2866393946808193E-4 64-65 5.144938638031843E-4 2.858299243351024E-5 0.0 1.6950143257958077 2.2866393946808193E-4 66-67 5.716598486702048E-4 2.858299243351024E-5 0.0 1.9056995630232116 2.2866393946808193E-4 68-69 5.716598486702048E-4 2.858299243351024E-5 0.0 2.1364357694427234 2.2866393946808193E-4 70-71 6.145343373204702E-4 2.858299243351024E-5 0.0 2.384879139674794 2.858299243351024E-4 72-73 6.574088259707356E-4 2.858299243351024E-5 0.0 2.6422261120499035 2.858299243351024E-4 74-75 6.859918184042458E-4 2.858299243351024E-5 0.0 2.90443219313871 2.858299243351024E-4 76-77 6.859918184042458E-4 4.287448865026536E-5 0.0 3.1948639792556075 2.858299243351024E-4 78-79 6.859918184042458E-4 5.716598486702048E-5 0.0 3.511306288486999 2.858299243351024E-4 80-81 7.14574810837756E-4 5.716598486702048E-5 0.0 3.8504149107181647 2.858299243351024E-4 82-83 7.431578032712663E-4 5.716598486702048E-5 0.0 4.202943247896863 3.287044129853678E-4 84-85 7.717407957047765E-4 5.716598486702048E-5 0.0 4.553213528673314 3.429959092021229E-4 86-87 8.146152843550419E-4 5.716598486702048E-5 0.0 4.927393482620397 3.429959092021229E-4 88-89 8.28906780571797E-4 5.716598486702048E-5 0.0 5.332543108869189 3.57287405418878E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5290 0.0 46.03061 1 GTATCAA 8770 0.0 42.08182 1 ATCAACG 11225 0.0 33.087658 3 TCAACGC 11330 0.0 32.697075 4 TATCAAC 11505 0.0 32.24106 2 CAACGCA 11545 0.0 32.04697 5 AACGCAG 11940 0.0 31.121155 6 ACGCAGA 13565 0.0 27.115131 7 CGCAGAG 13800 0.0 26.584517 8 GTACATG 11330 0.0 24.8079 1 TACATGG 11410 0.0 23.965336 2 GCAGAGT 14910 0.0 23.52173 9 ACATGGG 11660 0.0 23.084435 3 GAGTACT 8120 0.0 21.008938 12-13 CATGGGG 7555 0.0 20.3315 4 GTGTAGC 2125 0.0 20.142622 1 AGTACTT 8605 0.0 20.07332 12-13 GTACTTT 8585 0.0 19.865463 14-15 AGAGTAC 14330 0.0 19.83076 10-11 TAGCCCT 2145 0.0 19.731678 4 >>END_MODULE