FastQCFastQC Report
Thu 26 May 2016
SRR938647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498584
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75800.21665908264600764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64960.18567511884808255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43550.12447893204793711No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.045.3985331
GTATCAA86350.040.28891
ATCAACG110900.031.1424773
TCAACGC112700.030.7293854
TATCAAC114750.030.2757632
CAACGCA117100.029.5341685
AACGCAG120950.028.6574526
ACGCAGA139900.024.4702347
GTACATG107950.024.390671
CGCAGAG141350.024.0848488
TACATGG109050.023.795642
ACATGGG111200.022.4714583
GCAGAGT154950.021.5112729
GAGTACT84950.020.45682712-13
GGACCGA9150.020.2378376
GTGGTAT28500.020.1779541
CATGGGG75100.019.9892924
ATTCGCG5950.019.961380-81
GTACTTT89700.019.37355214-15
AGAGTAC148100.019.18799810-11
Produced by FastQC (version 0.11.5)