##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938647_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3498584 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78847785275414 31.0 31.0 34.0 30.0 34.0 2 32.0189996867304 31.0 31.0 34.0 30.0 34.0 3 32.08624603553895 33.0 31.0 34.0 30.0 34.0 4 35.754051925007374 37.0 35.0 37.0 35.0 37.0 5 35.64361095803331 37.0 35.0 37.0 33.0 37.0 6 35.640571442617926 37.0 35.0 37.0 33.0 37.0 7 35.57814618714314 37.0 35.0 37.0 33.0 37.0 8 35.55912820729758 37.0 35.0 37.0 33.0 37.0 9 37.142803774326985 39.0 37.0 39.0 33.0 39.0 10-11 37.04031659665739 39.0 37.0 39.0 33.0 39.0 12-13 36.92396652474258 39.0 37.0 39.0 33.0 39.0 14-15 37.980923853764835 40.0 37.0 41.0 33.0 41.0 16-17 37.88394704829154 40.0 37.0 41.0 33.0 41.0 18-19 38.11008453705842 40.0 37.0 41.0 33.0 41.0 20-21 38.07960177603282 40.0 37.0 41.0 33.0 41.0 22-23 37.96753572302394 40.0 37.0 41.0 33.0 41.0 24-25 37.84815285269698 40.0 37.0 41.0 32.0 41.0 26-27 37.6587144970651 40.0 37.0 41.0 32.0 41.0 28-29 37.54062543589063 39.5 36.5 41.0 32.0 41.0 30-31 37.311695531677955 39.0 36.0 41.0 31.0 41.0 32-33 37.142274417307114 39.0 36.0 41.0 31.0 41.0 34-35 37.06444578720991 39.0 36.0 41.0 31.0 41.0 36-37 37.03757420144836 39.0 36.0 41.0 31.0 41.0 38-39 36.89403927417493 39.0 36.0 41.0 30.0 41.0 40-41 36.73039578297963 39.0 35.0 41.0 30.0 41.0 42-43 36.53656908051943 39.0 35.0 40.0 30.0 41.0 44-45 36.25591968064794 39.0 35.0 40.0 30.0 41.0 46-47 35.8966247487555 38.0 35.0 40.0 28.5 41.0 48-49 35.57165841952058 38.0 34.5 40.0 28.0 41.0 50-51 35.210343527552865 38.0 34.0 40.0 27.0 41.0 52-53 34.71867918563625 38.0 33.0 40.0 26.0 41.0 54-55 34.37298304113893 38.0 33.0 40.0 24.5 41.0 56-57 34.03408907718094 37.0 33.0 40.0 24.0 41.0 58-59 33.54735901724812 37.0 32.0 39.5 23.0 41.0 60-61 33.137866634043945 36.0 31.5 39.0 22.0 40.0 62-63 32.641605861114094 36.0 31.0 39.0 21.5 40.0 64-65 32.10712662608644 35.0 31.0 38.5 19.5 40.0 66-67 31.582989289381075 35.0 30.0 38.0 18.0 40.0 68-69 31.0688467105549 34.0 29.0 37.5 15.5 39.5 70-71 30.486232001289665 34.0 29.0 37.0 11.5 39.0 72-73 29.950332763197913 34.0 28.5 36.0 8.0 39.0 74-75 29.32708718727348 33.5 28.0 36.0 7.0 38.5 76-77 28.10889033963455 31.5 26.0 34.5 6.5 36.5 78-79 28.792832900396277 33.0 28.0 35.0 2.0 37.0 80-81 28.398184665567555 33.0 27.0 35.0 2.0 36.5 82-83 27.937723090255943 33.0 26.0 35.0 2.0 36.0 84-85 27.355051357920807 32.5 26.0 35.0 2.0 36.0 86-87 26.708641410353447 32.0 25.0 34.5 2.0 35.0 88-89 25.9481720318849 31.5 23.5 34.0 2.0 35.0 90-91 25.314832515097535 31.0 21.0 34.0 2.0 35.0 92-93 24.447968092233886 31.0 18.0 34.0 2.0 35.0 94-95 23.596200348483844 30.5 8.0 34.0 2.0 35.0 96-97 22.481368176382215 30.0 2.0 34.0 2.0 35.0 98-99 21.08199274334988 29.0 2.0 34.0 2.0 35.0 100-101 17.15486265300476 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16641553730871195 1101 2 0.2638350039348296 1101 3 0.2754807962044197 1101 4 0.23024236831597023 1101 5 0.16315486508775479 1101 6 0.14045750460296347 1101 7 0.22824949023837604 1101 8 0.23139720340707015 1101 9 0.2558472294917209 1101 10-11 0.28609227326844433 1101 12-13 0.2799541991791372 1101 14-15 0.39169195009548474 1101 16-17 0.4766266779432584 1101 18-19 0.39434858522627536 1101 20-21 0.29547056447319875 1101 22-23 0.34766520586880745 1101 24-25 0.4366028581211765 1101 26-27 0.370295840468053 1101 28-29 0.30269185076777205 1101 30-31 0.4396801818834817 1101 32-33 0.3868815899528073 1101 34-35 0.40150363047547444 1101 36-37 0.5984035381101265 1101 38-39 0.5797065843900739 1101 40-41 0.6465402831103262 1101 42-43 0.7229875085009354 1101 44-45 0.6467607825807917 1101 46-47 0.7431125899946878 1101 48-49 0.7828533730084857 1101 50-51 0.861887554859976 1101 52-53 0.8851300643938416 1101 54-55 0.8535973274879112 1101 56-57 0.7798211199788625 1101 58-59 0.900758665459513 1101 60-61 0.8957785444221429 1101 62-63 0.8997023699620499 1101 64-65 0.7497044034814593 1101 66-67 0.7710407131462453 1101 68-69 0.9339141582601265 1101 70-71 0.6688983806890256 1101 72-73 0.8806539576766284 1101 74-75 0.7673489031867575 1101 76-77 0.7562744738372729 1101 78-79 0.8471086253952151 1101 80-81 0.8511203509090883 1101 82-83 0.8351917439279148 1101 84-85 1.0005444367155967 1101 86-87 1.0683203126749206 1101 88-89 1.21408344031083 1101 90-91 1.2723868803732614 1101 92-93 1.3492041321874737 1101 94-95 1.3395904857313887 1101 96-97 1.3544346468215949 1101 98-99 1.6516109518455302 1101 100-101 1.7368341806151975 1102 1 0.3389429200043992 1102 2 0.31130251457959446 1102 3 0.24329107875609424 1102 4 0.23592166000412362 1102 5 0.1821800986467892 1102 6 0.21484124887437162 1102 7 0.22229972725398284 1102 8 0.28855515561640743 1102 9 0.2960210981294651 1102 10-11 0.4312901966947891 1102 12-13 0.3757375213942211 1102 14-15 0.5350766561547431 1102 16-17 0.4660187533361224 1102 18-19 0.4977379034266747 1102 20-21 0.5162545174515571 1102 22-23 0.4831905004857262 1102 24-25 0.5098678806318588 1102 26-27 0.4880370922859072 1102 28-29 0.5030182833825663 1102 30-31 0.5076505269261418 1102 32-33 0.5559692943075021 1102 34-35 0.5316254031120522 1102 36-37 0.7474404887279249 1102 38-39 0.6937863310351773 1102 40-41 0.6740475167036735 1102 42-43 0.4842952271260117 1102 44-45 0.6035298313796673 1102 46-47 0.7940572210889911 1102 48-49 0.75218995132456 1102 50-51 0.7686734071927432 1102 52-53 0.7573213259506915 1102 54-55 0.7609454674426814 1102 56-57 0.8927646348744673 1102 58-59 1.0218859050476183 1102 60-61 0.9904413787392983 1102 62-63 0.981625905304476 1102 64-65 0.9871457551112996 1102 66-67 0.8904103939194705 1102 68-69 1.1263087173465003 1102 70-71 1.1314939160960407 1102 72-73 1.1148235653822347 1102 74-75 1.1054005309092574 1102 76-77 1.0184214608576774 1102 78-79 1.3059279071728156 1102 80-81 1.2798681697296495 1102 82-83 1.223750606252981 1102 84-85 1.1329221216225882 1102 86-87 1.0467703103032484 1102 88-89 1.1432308602871544 1102 90-91 1.1095287455957994 1102 92-93 1.3564336381837627 1102 94-95 1.5619642144910664 1102 96-97 1.6502188268747418 1102 98-99 1.9078982488785208 1102 100-101 2.0410336511777274 1103 1 0.18713914069322612 1103 2 0.22348562167769614 1103 3 0.15344212663541867 1103 4 0.10415517553357745 1103 5 0.0693696228733316 1103 6 0.06723993374051673 1103 7 0.15992448378058555 1103 8 0.15386472544231822 1103 9 0.20583222694010317 1103 10-11 0.20844432290837744 1103 12-13 0.26164055292119315 1103 14-15 0.3825891223704474 1103 16-17 0.2790715228932612 1103 18-19 0.26228006018579464 1103 20-21 0.269021479431963 1103 22-23 0.25932449201511787 1103 24-25 0.2872114065042126 1103 26-27 0.3219612418276441 1103 28-29 0.31304636775648476 1103 30-31 0.2800614987058765 1103 32-33 0.32481394695210497 1103 34-35 0.3987287490783231 1103 36-37 0.40314729767867874 1103 38-39 0.44206083125411766 1103 40-41 0.4274566633765886 1103 42-43 0.4480502947700984 1103 44-45 0.4405360427825329 1103 46-47 0.3899102933836076 1103 48-49 0.4813949162945974 1103 50-51 0.6292684637389598 1103 52-53 0.4785420147728061 1103 54-55 0.4397603087508699 1103 56-57 0.5691288880717025 1103 58-59 0.43194536729225774 1103 60-61 0.5170920906630769 1103 62-63 0.5812614973411954 1103 64-65 0.4418535209691541 1103 66-67 0.5580713222892193 1103 68-69 0.5061591227580244 1103 70-71 0.42267569635846414 1103 72-73 0.34373527701913176 1103 74-75 0.3395482890665562 1103 76-77 0.36807380348703944 1103 78-79 0.7169924993454302 1103 80-81 0.7913892273479171 1103 82-83 0.7015012894884229 1103 84-85 0.7987988051751138 1103 86-87 0.776711168915039 1103 88-89 0.7567969057506225 1103 90-91 0.8644692739142101 1103 92-93 1.297555682529893 1103 94-95 1.2046373165309596 1103 96-97 1.184632046536823 1103 98-99 1.3450254420265395 1103 100-101 1.3887391502793314 1104 1 0.16448648919752884 1104 2 0.20734363209989226 1104 3 0.22360555848305097 1104 4 0.1663455347169247 1104 5 0.15496675876712374 1104 6 0.10909602353350323 1104 7 0.15006877354731074 1104 8 0.1373306906369791 1104 9 0.24339740133780197 1104 10-11 0.20965586394935087 1104 12-13 0.25752131338191475 1104 14-15 0.35499015502971076 1104 16-17 0.34903491892529814 1104 18-19 0.27113585888786673 1104 20-21 0.2830199733219061 1104 22-23 0.24297319166637266 1104 24-25 0.3484096130132315 1104 26-27 0.2979394642325204 1104 28-29 0.33409052795921923 1104 30-31 0.41017717166809575 1104 32-33 0.4613787460708494 1104 34-35 0.4328159935889815 1104 36-37 0.4601991532405165 1104 38-39 0.24247404835085007 1104 40-41 0.45768508055550683 1104 42-43 0.46343735649278983 1104 44-45 0.4214145629848076 1104 46-47 0.38904032040939995 1104 48-49 0.42261961350296673 1104 50-51 0.5581780714511595 1104 52-53 0.48307746793285133 1104 54-55 0.6957473816035034 1104 56-57 0.5276724239986024 1104 58-59 0.621783458677676 1104 60-61 0.739798037479062 1104 62-63 0.6636524417028724 1104 64-65 0.6054895067970207 1104 66-67 0.6520616684876224 1104 68-69 0.6565707167375834 1104 70-71 0.8273716954281163 1104 72-73 0.580492602806931 1104 74-75 0.6427439927645509 1104 76-77 0.7443394781615602 1104 78-79 0.9795004008939188 1104 80-81 0.871250606005816 1104 82-83 0.9993186784600709 1104 84-85 1.0978371885075902 1104 86-87 1.1129587110518742 1104 88-89 1.1757631370651538 1104 90-91 1.4926643176634613 1104 92-93 1.6089047854276757 1104 94-95 1.656327195557001 1104 96-97 1.55862294923255 1104 98-99 1.6309704422548421 1104 100-101 1.7346161472485626 1105 1 0.18710426336425456 1105 2 0.19862624151549824 1105 3 0.18038677197636588 1105 4 0.17471131796911976 1105 5 0.05689395024408839 1105 6 0.12635874153971116 1105 7 0.15572046424649955 1105 8 0.14857985574818144 1105 9 0.2426242518657702 1105 10-11 0.2198232250047667 1105 12-13 0.2624289801028823 1105 14-15 0.30651251475116226 1105 16-17 0.21965161616321893 1105 18-19 0.2833267062707918 1105 20-21 0.20866569770782206 1105 22-23 0.18513542461753474 1105 24-25 0.25851148583711847 1105 26-27 0.11426397149052292 1105 28-29 0.14645489267797984 1105 30-31 0.30828341777104384 1105 32-33 0.3143974248846533 1105 34-35 0.3371987205656737 1105 36-37 0.2531512712649189 1105 38-39 0.18557186949493598 1105 40-41 0.24624946185016938 1105 42-43 0.3596799492450842 1105 44-45 0.261253514554447 1105 46-47 0.2607014586958982 1105 48-49 0.3307302776028962 1105 50-51 0.3895866005052895 1105 52-53 0.4150330014992889 1105 54-55 0.392000968914509 1105 56-57 0.3613766555756115 1105 58-59 0.3211028299301333 1105 60-61 0.33393718118449556 1105 62-63 0.28900045562998855 1105 64-65 0.32656768130353697 1105 66-67 0.262850045591577 1105 68-69 0.525812757912707 1105 70-71 0.5520883291699441 1105 72-73 0.4714556219194783 1105 74-75 0.49604854691586553 1105 76-77 0.3653186680172489 1105 78-79 0.6495841226091983 1105 80-81 0.6639804792809194 1105 82-83 0.7458131962267878 1105 84-85 0.8734518625113594 1105 86-87 0.7534627829892386 1105 88-89 0.7457630462843916 1105 90-91 0.8150455827336955 1105 92-93 1.0854362358335585 1105 94-95 1.2796381060426327 1105 96-97 1.3023602535853058 1105 98-99 1.4456501366330095 1105 100-101 1.4550288063229289 1106 1 0.0019036245536057095 1106 2 0.023412616793869745 1106 3 -0.018040542243603852 1106 4 0.011732640658138394 1106 5 -0.06138591105114699 1106 6 -0.038002637433983466 1106 7 0.006398929967353695 1106 8 0.05208900962978191 1106 9 -0.15014118203031046 1106 10-11 0.08038005511721025 1106 12-13 0.01958145578331738 1106 14-15 0.012420918346698784 1106 16-17 0.05625598014648858 1106 18-19 0.0634418617303325 1106 20-21 0.06565505838242558 1106 22-23 0.10511293950069245 1106 24-25 0.22533428859115645 1106 26-27 0.05905967990182859 1106 28-29 0.05223238784719797 1106 30-31 0.09997621062762363 1106 32-33 0.0674322835908896 1106 34-35 0.11961919125929654 1106 36-37 0.2165694138805776 1106 38-39 0.0776578916328674 1106 40-41 0.01900130751108975 1106 42-43 0.13769779373919278 1106 44-45 -0.021548097625291973 1106 46-47 -0.5920717979783987 1106 48-49 -0.2724316599376593 1106 50-51 0.19486415834431625 1106 52-53 0.1973382126279617 1106 54-55 0.36326704855333247 1106 56-57 0.08447030161737246 1106 58-59 0.09161624395397894 1106 60-61 0.2149423387814977 1106 62-63 0.07255670812262593 1106 64-65 0.12258378194934494 1106 66-67 0.07439227062335974 1106 68-69 -0.09075626310889717 1106 70-71 0.013670787639114224 1106 72-73 0.41463443279934964 1106 74-75 0.28463135579063703 1106 76-77 0.10794534013815493 1106 78-79 0.3835521161434272 1106 80-81 -0.03918627520059559 1106 82-83 0.5662402812495984 1106 84-85 0.531804086983783 1106 86-87 0.7947459086501745 1106 88-89 0.886376410593769 1106 90-91 0.8166813183875803 1106 92-93 0.9440494757121805 1106 94-95 1.2919827772541872 1106 96-97 1.3744842361924299 1106 98-99 1.2967094546582771 1106 100-101 1.1122243866019588 1107 1 0.1763898007170681 1107 2 0.13146286150055175 1107 3 0.1809448944773564 1107 4 0.10554441927399694 1107 5 0.09183970888934567 1107 6 0.11455672389551808 1107 7 0.1526828511796623 1107 8 0.13069469478314488 1107 9 0.15918078340408215 1107 10-11 0.15607089844917255 1107 12-13 0.2003461609947763 1107 14-15 0.26299173691653266 1107 16-17 0.29779836883092514 1107 18-19 0.28638956027095475 1107 20-21 0.2165043401030502 1107 22-23 0.27876458952831484 1107 24-25 0.18227692983924726 1107 26-27 0.19118869456142562 1107 28-29 0.25648259029097176 1107 30-31 0.2864155589170849 1107 32-33 0.3001788230379532 1107 34-35 0.3284721381943214 1107 36-37 0.3705107153247411 1107 38-39 0.26936612421953754 1107 40-41 0.18441485831782245 1107 42-43 0.1867260953151515 1107 44-45 0.13520448725535772 1107 46-47 0.2513598441415681 1107 48-49 0.4086006734058216 1107 50-51 0.28441931112441665 1107 52-53 0.29869217019777494 1107 54-55 0.22279609907619857 1107 56-57 0.34135279583869504 1107 58-59 0.20340678783390587 1107 60-61 0.28603051682557634 1107 62-63 0.26903428945497154 1107 64-65 0.19489149363139546 1107 66-67 0.3600921130403698 1107 68-69 0.42515450259110565 1107 70-71 0.5829989483598261 1107 72-73 0.7282696940690379 1107 74-75 0.7225497475063491 1107 76-77 0.8140396738668727 1107 78-79 0.9020493465605561 1107 80-81 0.8526849499675713 1107 82-83 1.033465339479907 1107 84-85 1.2354910743796772 1107 86-87 1.3763800924374365 1107 88-89 1.2401885234477774 1107 90-91 1.4683232276699592 1107 92-93 1.7339405615964942 1107 94-95 1.8705215355737117 1107 96-97 2.0120810543719436 1107 98-99 2.1051480254876154 1107 100-101 1.9494653361931427 1108 1 0.12410110145240694 1108 2 0.11855194869874452 1108 3 0.1461322026818621 1108 4 0.18680961816230734 1108 5 0.06075164942550515 1108 6 0.20180648505584742 1108 7 0.14581530587250313 1108 8 0.14677475673671125 1108 9 0.2193783327642791 1108 10-11 0.20576764443956108 1108 12-13 0.19849153474267922 1108 14-15 0.35009131858522125 1108 16-17 0.34746842629719765 1108 18-19 0.2888729500573035 1108 20-21 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68-69 -1.0035915815615617 2316 70-71 -1.014671857878323 2316 72-73 -1.0407992477831947 2316 74-75 -0.9437425725212023 2316 76-77 -1.067241401930545 2316 78-79 -1.211424339269204 2316 80-81 -1.2286779443698599 2316 82-83 -1.1126927514541158 2316 84-85 -1.309290020038933 2316 86-87 -1.5704329988528123 2316 88-89 -1.4607836713374276 2316 90-91 -1.3631156357249914 2316 92-93 -1.6048637583036758 2316 94-95 -1.613354875318766 2316 96-97 -1.7587846636395525 2316 98-99 -1.5709442354918686 2316 100-101 -1.8194776427546575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 22.0 4 44.0 5 121.0 6 232.0 7 588.0 8 1414.0 9 2445.0 10 4070.0 11 6270.0 12 9050.0 13 12721.0 14 17102.0 15 22217.0 16 27701.0 17 33933.0 18 33617.0 19 30979.0 20 31882.0 21 34830.0 22 38102.0 23 42880.0 24 49756.0 25 57897.0 26 68569.0 27 82020.0 28 97673.0 29 114717.0 30 136132.0 31 161411.0 32 192423.0 33 235774.0 34 293957.0 35 378938.0 36 490585.0 37 538663.0 38 241682.0 39 8162.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.11057660701372 19.020142515370566 14.929271102193182 23.94000977542253 2 16.15592672432283 19.963240908459166 39.280838429865746 24.599993937352256 3 19.551338484369676 23.25094952700864 29.38937581604443 27.808336172577246 4 13.703984558310928 16.76628324454972 35.731114986333026 33.79861721080633 5 14.578526207906881 36.481088390865374 33.804463795750564 15.135921605477185 6 33.432935562769266 35.39224308813026 16.866142664044272 14.308678685056206 7 29.943885869254533 31.46833118770337 19.792807604447972 18.794975338594128 8 26.539251308529394 35.57544995346689 18.85860107975112 19.026697658252594 9 26.233984949339508 16.844443351938956 19.454242059073042 37.4673296396485 10-11 25.639501581211142 26.116194437521006 26.937498142105493 21.306805839162358 12-13 26.155224513688967 23.119753591738828 27.408059946538373 23.316961948033832 14-15 22.808999298001705 24.952623690041456 25.34312453266807 26.89525247928876 16-17 22.994688708346008 28.16966807142547 25.932462962158404 22.903180258070122 18-19 22.825048648253123 26.80132876615225 27.93688818104696 22.436734404547668 20-21 23.447443308492808 26.060786306688648 28.00154290993156 22.490227474886986 22-23 23.170674192759126 25.791434477491464 27.92721283810822 23.11067849164119 24-25 23.021183883794922 26.053227119967687 27.954428451903418 22.971160544333973 26-27 22.8980371347023 26.425972482470154 27.823743862964296 22.85224651986325 28-29 22.758471072431323 26.480778075009226 27.953359974595426 22.80739087796402 30-31 23.020458621847776 26.101357000450186 27.916807797571835 22.961376580130196 32-33 22.839508731201448 26.10997024930304 27.90733137377277 23.14318964572275 34-35 23.268691369713267 26.26355271523118 27.592825093648376 22.874930821407172 36-37 23.275652700580324 26.041924418372776 27.724756271416172 22.957666609630724 38-39 22.912166455573 26.269241752126014 27.77472404183914 23.043867750461846 40-41 23.216797208563815 26.059554544980323 27.732929928977875 22.99071831747799 42-43 23.133721389725707 25.9455462432756 27.686369182173227 23.23436318482547 44-45 23.128415238064747 26.229071215324506 27.49285937251664 23.1496541740941 46-47 23.11616642618842 26.50376552342319 27.094733183482234 23.285334866906155 48-49 23.2820478227763 26.27660219105787 27.239663246616345 23.201686739549487 50-51 23.250212980392085 26.454472788315996 27.096293719575886 23.19902051171603 52-53 23.373284734624065 26.478741113547656 26.940485064814794 23.20748908701349 54-55 23.130545636700013 26.32889122638024 27.116293926516228 23.42426921040352 56-57 23.140461969756906 26.578795878561156 27.094904681436834 23.185837470245104 58-59 23.297615797290593 26.311325784811373 27.00277173035166 23.388286687546366 60-61 23.264097703528055 26.370311531751135 27.255512515920728 23.110078248800086 62-63 23.030203067015716 26.444306216758417 27.083725195483233 23.44176552074263 64-65 22.939154425716765 26.28107775723147 27.061052831990757 23.718714985061006 66-67 22.915854382219802 26.46627109128724 27.040439473994134 23.577435052498824 68-69 22.860768167642004 26.208585386241467 27.185951045811414 23.744695400305115 70-71 22.894903074961842 26.30112788617096 27.016275174499366 23.787693864367828 72-73 23.118409115464495 26.244232663404777 26.84373268941531 23.79362553171542 74-75 22.959090903892843 26.446864947264054 26.66936169093523 23.924682457907874 76-77 22.866302838409467 26.360095033069403 26.882245013280382 23.89135711524075 78-79 22.981052218656377 26.32465135787834 26.905675099323567 23.78862132414171 80-81 22.739059126554192 26.57026073459359 26.871603726532094 23.819076412320126 82-83 22.957997999326054 26.541240203395656 26.68217663145925 23.81858516581904 84-85 22.924212262325796 26.243928920756897 26.68260321245658 24.149255604460727 86-87 22.844072954193166 26.165059540952583 26.879665315742496 24.111202189111754 88-89 22.886410550983605 26.20403225437336 26.750816428813355 24.158740765829677 90-91 22.755051176843686 26.380451061826054 26.820122625438337 24.044375135891922 92-93 22.826722458771318 25.99590536422446 26.817276949990955 24.360095227013275 94-95 22.615737246344988 26.063624178880534 26.827962891804358 24.49267568297012 96-97 22.605151886654205 26.07111991164518 26.833487620674738 24.490240581025873 98-99 22.69865321718898 25.92783193376803 26.557489753672026 24.81602509537096 100-101 22.68093312689675 25.79933666620165 26.726385957128578 24.79334424977302 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 372.0 1 263.0 2 209.5 3 273.0 4 244.0 5 259.5 6 465.0 7 1061.0 8 1711.0 9 1978.5 10 1856.5 11 1672.5 12 1742.5 13 1944.0 14 2187.0 15 2424.0 16 2721.5 17 2931.5 18 3147.0 19 3449.0 20 3852.0 21 4515.5 22 5421.0 23 6704.5 24 8214.5 25 10181.5 26 13240.0 27 17064.0 28 20669.5 29 24654.0 30 29522.0 31 35226.5 32 41761.5 33 47723.5 34 53254.5 35 58915.5 36 66378.5 37 73740.0 38 79856.0 39 85193.5 40 91281.0 41 101531.0 42 110601.5 43 117173.5 44 124398.0 45 133212.5 46 139443.0 47 142993.0 48 146523.5 49 146464.5 50 144589.0 51 141424.5 52 137419.5 53 135288.0 54 133818.5 55 126627.5 56 114332.5 57 103583.5 58 92950.0 59 79642.0 60 65875.0 61 53969.0 62 44089.5 63 35413.0 64 28362.0 65 22229.5 66 16555.5 67 13279.5 68 10239.0 69 7078.0 70 5190.5 71 3867.5 72 3133.0 73 2588.0 74 2091.5 75 1800.0 76 1224.0 77 636.5 78 357.0 79 212.5 80 120.5 81 71.0 82 39.0 83 19.5 84 11.0 85 9.0 86 6.5 87 3.5 88 1.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.0016189406914343E-4 2 0.05036323266784505 3 0.0 4 8.574897730053072E-5 5 0.045875702855783934 6 2.8582992433510245E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006202509358071722 26-27 0.0014434411178922673 28-29 2.8582992433510245E-5 30-31 0.0016863965535771045 32-33 3.0012142055185753E-4 34-35 0.011204533033936016 36-37 0.022566272526256335 38-39 0.003915869963390903 40-41 7.574492994880214E-4 42-43 0.001071862216256634 44-45 0.008346233790584991 46-47 0.0 48-49 0.0 50-51 6.431173297539804E-4 52-53 0.0 54-55 0.0016149390724933287 56-57 0.0 58-59 0.02198032118136938 60-61 0.0 62-63 0.00943238750305838 64-65 0.014963196538942613 66-67 0.006288258335372253 68-69 0.01596360127411547 70-71 1.1433196973404098E-4 72-73 5.716598486702049E-5 74-75 0.022294734098137987 76-77 0.04847675516723337 78-79 0.03002643355140251 80-81 0.025967648625844053 82-83 0.036086027947306684 84-85 0.015563439380046328 86-87 0.010332751764713953 88-89 0.08209035426904142 90-91 0.024567081996602055 92-93 0.023923964666848072 94-95 0.02629635303882942 96-97 0.037772424500883785 98-99 0.05269274655117614 100-101 0.01417716424702108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3498584.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.13769864615454 #Duplication Level Percentage of deduplicated Percentage of total 1 73.36518263524961 36.78361418086235 2 14.325544324492995 14.364996485671186 3 5.036866575868233 7.576106955053093 4 2.3748824994069397 4.762845723011657 5 1.2954698591286629 3.247593870108459 6 0.7927643984363056 2.384842950371969 7 0.5415830929937564 1.9007610935861272 8 0.36424570563768766 1.4609953137934637 9 0.2789108893463663 1.258555510726116 >10 1.4434560363099853 13.044898921776705 >50 0.10534701666555235 3.6940178674350657 >100 0.07060918870230526 6.863779235959486 >500 0.004053135792556467 1.3665486532564142 >1k 9.704691301256005E-4 0.8875625431932601 >5k 1.1417283883830592E-4 0.402880695194722 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7580 0.21665908264600764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6496 0.18567511884808255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4355 0.12447893204793711 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.858299243351024E-5 0.0 0.0 0.0 0.0 3 2.858299243351024E-5 0.0 0.0 0.0 0.0 4 2.858299243351024E-5 0.0 0.0 0.0 0.0 5 5.716598486702048E-5 0.0 0.0 0.0 0.0 6 1.429149621675512E-4 0.0 0.0 0.0 0.0 7 1.7149795460106145E-4 0.0 0.0 0.0 0.0 8 1.7149795460106145E-4 0.0 0.0 0.0 0.0 9 1.7149795460106145E-4 0.0 0.0 0.0 5.716598486702048E-5 10-11 1.7149795460106145E-4 0.0 0.0 0.0 5.716598486702048E-5 12-13 1.7149795460106145E-4 0.0 0.0 0.0 5.716598486702048E-5 14-15 1.7149795460106145E-4 0.0 0.0 1.429149621675512E-5 5.716598486702048E-5 16-17 1.7149795460106145E-4 0.0 0.0 4.287448865026536E-5 5.716598486702048E-5 18-19 1.8578945081781657E-4 0.0 0.0 8.574897730053072E-5 5.716598486702048E-5 20-21 2.000809470345717E-4 0.0 0.0 8.574897730053072E-5 7.14574810837756E-5 22-23 2.000809470345717E-4 0.0 0.0 2.2866393946808193E-4 1.0004047351728584E-4 24-25 3.1441291676861266E-4 0.0 0.0 4.5732787893616386E-4 1.429149621675512E-4 26-27 3.1441291676861266E-4 0.0 0.0 7.860322919215316E-4 1.429149621675512E-4 28-29 4.001618940691434E-4 0.0 0.0 0.0023295138833310846 1.429149621675512E-4 30-31 4.5732787893616386E-4 0.0 0.0 0.007960363392732603 2.000809470345717E-4 32-33 4.5732787893616386E-4 0.0 0.0 0.021708782753251027 3.1441291676861266E-4 34-35 4.859108713696741E-4 0.0 0.0 0.042202788328077875 3.429959092021229E-4 36-37 5.430768562366946E-4 0.0 0.0 0.07787436288509866 3.429959092021229E-4 38-39 5.716598486702048E-4 0.0 0.0 0.13213917402011785 3.429959092021229E-4 40-41 5.716598486702048E-4 0.0 0.0 0.19214916663427262 3.429959092021229E-4 42-43 6.431173297539804E-4 0.0 0.0 0.25378838981713747 3.57287405418878E-4 44-45 7.431578032712663E-4 0.0 0.0 0.33337773224824674 3.7157890163563314E-4 46-47 7.717407957047765E-4 0.0 0.0 0.428316141616151 3.7157890163563314E-4 48-49 7.717407957047765E-4 0.0 0.0 0.5313149548503051 3.7157890163563314E-4 50-51 7.717407957047765E-4 0.0 0.0 0.6396159131808754 3.7157890163563314E-4 52-53 7.717407957047765E-4 0.0 0.0 0.74807407796983 4.001618940691434E-4 54-55 7.717407957047765E-4 0.0 0.0 0.8699233747138841 4.287448865026536E-4 56-57 8.003237881382868E-4 0.0 0.0 1.0142960694955445 4.287448865026536E-4 58-59 8.003237881382868E-4 0.0 0.0 1.1735176288464133 4.287448865026536E-4 60-61 8.146152843550419E-4 0.0 0.0 1.344472506591238 4.287448865026536E-4 62-63 8.28906780571797E-4 0.0 0.0 1.5146270605479244 4.5732787893616386E-4 64-65 8.28906780571797E-4 0.0 0.0 1.700373636877091 4.5732787893616386E-4 66-67 8.28906780571797E-4 0.0 0.0 1.9121879023056185 4.5732787893616386E-4 68-69 8.28906780571797E-4 0.0 0.0 2.1445533392938403 4.5732787893616386E-4 70-71 8.431982767885521E-4 0.0 0.0 2.3952547659281587 4.8591087136967416E-4 72-73 8.717812692220624E-4 0.0 0.0 2.655574369516353 5.430768562366946E-4 74-75 8.860727654388175E-4 0.0 0.0 2.9219106930118013 5.430768562366946E-4 76-77 8.860727654388175E-4 0.0 0.0 3.2172587538272626 5.430768562366946E-4 78-79 8.860727654388175E-4 0.0 0.0 3.540489523761613 5.430768562366946E-4 80-81 9.43238750305838E-4 0.0 0.0 3.886472355673038 5.716598486702048E-4 82-83 9.43238750305838E-4 0.0 0.0 4.2484616633472285 6.002428411037151E-4 84-85 9.861132389561034E-4 0.0 0.0 4.608821740452709 6.002428411037151E-4 86-87 0.0010289877276063687 0.0 0.0 4.9946635553126635 6.145343373204702E-4 88-89 0.0010289877276063687 0.0 0.0 5.4164770661501915 6.288258335372253E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5360 0.0 45.398533 1 GTATCAA 8635 0.0 40.2889 1 ATCAACG 11090 0.0 31.142477 3 TCAACGC 11270 0.0 30.729385 4 TATCAAC 11475 0.0 30.275763 2 CAACGCA 11710 0.0 29.534168 5 AACGCAG 12095 0.0 28.657452 6 ACGCAGA 13990 0.0 24.470234 7 GTACATG 10795 0.0 24.39067 1 CGCAGAG 14135 0.0 24.084848 8 TACATGG 10905 0.0 23.79564 2 ACATGGG 11120 0.0 22.471458 3 GCAGAGT 15495 0.0 21.511272 9 GAGTACT 8495 0.0 20.456827 12-13 GGACCGA 915 0.0 20.237837 6 GTGGTAT 2850 0.0 20.177954 1 CATGGGG 7510 0.0 19.989292 4 ATTCGCG 595 0.0 19.9613 80-81 GTACTTT 8970 0.0 19.373552 14-15 AGAGTAC 14810 0.0 19.187998 10-11 >>END_MODULE