FastQCFastQC Report
Thu 26 May 2016
SRR938646_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938646_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481170
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75170.21593314891257825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60980.17517099136209952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40320.11582312843095857No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55850.046.019671
GTATCAA89550.042.3077961
ATCAACG115150.032.482733
TATCAAC116300.032.24282
TCAACGC117700.031.7789864
CAACGCA119150.031.3899925
AACGCAG124100.030.1323016
ACGCAGA141100.026.3637947
CGCAGAG142900.025.8326058
GTACATG110450.025.5110841
TACATGG111300.024.3396992
GCAGAGT153350.023.2340939
ACATGGG114400.023.0157683
GAGTACT85050.020.88883812-13
GTACTTT87750.020.21961814-15
CATGGGG77050.020.1088244
CAGAGTA150100.019.84298310-11
GTGGTAT29000.019.5308821
AGTACTT90000.019.5288312-13
AGAGTAC146500.019.40647510-11
Produced by FastQC (version 0.11.5)