##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938646_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3481170 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.980622894027007 31.0 31.0 34.0 28.0 34.0 2 31.08037096723228 31.0 31.0 34.0 27.0 34.0 3 31.15472183202774 31.0 31.0 34.0 27.0 34.0 4 34.77255376784242 37.0 35.0 37.0 32.0 37.0 5 34.785108742175765 37.0 35.0 37.0 32.0 37.0 6 34.8114837827512 37.0 35.0 37.0 32.0 37.0 7 34.72874062456013 37.0 35.0 37.0 31.0 37.0 8 34.68997291140622 37.0 35.0 37.0 30.0 37.0 9 36.182199662757064 39.0 35.0 39.0 31.0 39.0 10-11 36.02583599766745 39.0 35.0 39.0 30.0 39.0 12-13 35.86835546669654 38.0 35.0 39.0 30.0 39.0 14-15 36.85268314963072 39.0 36.0 41.0 30.5 41.0 16-17 36.69659669019324 39.0 36.0 41.0 30.0 41.0 18-19 36.66370602412407 39.0 36.0 40.5 30.0 41.0 20-21 36.7546210038579 39.0 36.0 41.0 30.0 41.0 22-23 36.668459311093685 39.0 36.0 41.0 30.0 41.0 24-25 36.55350844687275 39.0 36.0 41.0 30.0 41.0 26-27 36.366751551920764 39.0 35.5 41.0 29.0 41.0 28-29 36.17223103152101 39.0 35.0 40.5 29.0 41.0 30-31 35.971856301186094 39.0 35.0 40.0 27.5 41.0 32-33 35.71398351703594 38.0 35.0 40.0 27.0 41.0 34-35 35.46842498355438 38.0 34.0 40.0 26.5 41.0 36-37 35.1852055487092 38.0 34.0 40.0 25.5 41.0 38-39 34.94232097254658 38.0 34.0 40.0 25.0 41.0 40-41 34.894914066247836 38.0 34.0 40.0 25.0 41.0 42-43 34.73789142730749 38.0 33.5 40.0 24.0 41.0 44-45 34.46603713693959 38.0 33.0 40.0 24.0 41.0 46-47 34.11820135184435 38.0 33.0 40.0 23.0 41.0 48-49 33.778615092052384 38.0 33.0 40.0 21.5 41.0 50-51 33.04932077433736 37.5 31.5 39.5 20.0 40.5 52-53 33.11387665641149 37.0 32.0 39.5 20.0 40.0 54-55 33.24213741357073 37.0 32.0 40.0 19.0 41.0 56-57 32.93845503092351 37.0 31.5 40.0 17.5 41.0 58-59 32.6034192527225 37.0 31.0 40.0 14.5 41.0 60-61 32.147279362972796 36.0 31.0 39.0 11.0 41.0 62-63 31.60712088177251 35.5 30.0 39.0 8.0 40.0 64-65 31.111277817515376 35.0 29.5 39.0 7.0 40.0 66-67 30.566042163985095 35.0 29.0 38.0 2.0 40.0 68-69 29.9965867797321 34.5 28.0 38.0 2.0 40.0 70-71 29.376969237354107 34.0 27.5 37.0 2.0 39.0 72-73 28.80315210116139 34.0 26.5 36.5 2.0 39.0 74-75 28.177226622083953 33.5 26.0 36.0 2.0 39.0 76-77 27.436155373049864 33.0 25.5 35.5 2.0 37.5 78-79 26.650841527417505 32.0 23.5 35.0 2.0 37.0 80-81 25.99199938526415 32.0 21.5 35.0 2.0 36.5 82-83 25.328941850010196 31.0 19.5 35.0 2.0 36.0 84-85 24.666649431082078 31.0 17.5 34.0 2.0 35.5 86-87 23.980344108446296 31.0 9.5 34.0 2.0 35.0 88-89 23.201212092486145 30.0 4.5 34.0 2.0 35.0 90-91 22.375912408759124 29.5 2.0 34.0 2.0 35.0 92-93 21.52875225857973 29.0 2.0 34.0 2.0 35.0 94-95 20.56117066963119 28.0 2.0 34.0 2.0 35.0 96-97 19.326213169710186 26.0 2.0 33.5 2.0 35.0 98-99 17.863349678412717 24.0 2.0 33.0 2.0 34.0 100-101 14.501320963928794 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5177215916487228 1101 2 0.6520609911443813 1101 3 0.6343874124060491 1101 4 0.5350206660785943 1101 5 0.514216383145623 1101 6 0.5246250656286975 1101 7 0.4137226048817553 1101 8 0.5109002615745482 1101 9 0.5360181224991933 1101 10-11 0.4904417892711521 1101 12-13 0.5088971250599243 1101 14-15 0.6887315843146382 1101 16-17 0.725106927452309 1101 18-19 0.6048357796206858 1101 20-21 0.7326315268285342 1101 22-23 0.7857938372012683 1101 24-25 0.8190103791593515 1101 26-27 0.8879106012915514 1101 28-29 0.8035851397938316 1101 30-31 0.860520844564256 1101 32-33 0.849936516451443 1101 34-35 0.7626606387290877 1101 36-37 0.8431941745006313 1101 38-39 0.7007634043091855 1101 40-41 0.7470952613077557 1101 42-43 0.9749075678532861 1101 44-45 0.9112603998345676 1101 46-47 0.8670809424865524 1101 48-49 0.9429915443175645 1101 50-51 0.8024068375842788 1101 52-53 0.9728159543848065 1101 54-55 0.9694766756891369 1101 56-57 0.8384521101749058 1101 58-59 0.6894309761603594 1101 60-61 0.7515194764120494 1101 62-63 0.7052661400287157 1101 64-65 0.7191365849196814 1101 66-67 0.7077400305998971 1101 68-69 0.7954080036265267 1101 70-71 0.8616105382308668 1101 72-73 0.8762695217177985 1101 74-75 0.6276888340286959 1101 76-77 0.8116260624798031 1101 78-79 0.6980835957097433 1101 80-81 0.8957116293871614 1101 82-83 0.906263263511164 1101 84-85 1.0964453325457733 1101 86-87 1.091929413527442 1101 88-89 1.1185722686354964 1101 90-91 1.2406107145457206 1101 92-93 1.018925530359958 1101 94-95 0.7905538956485643 1101 96-97 0.7382425843664606 1101 98-99 0.8948853981285829 1101 100-101 1.0000964144148146 1102 1 0.6139910635037467 1102 2 0.7083900422211507 1102 3 0.5441540604542077 1102 4 0.5604048279354785 1102 5 0.612205274103232 1102 6 0.5990994031201637 1102 7 0.4306927532091933 1102 8 0.4431270349873202 1102 9 0.563434804681286 1102 10-11 0.673993890059343 1102 12-13 0.7333303466822159 1102 14-15 0.6556402915696395 1102 16-17 0.7591432645713994 1102 18-19 0.9284524245657622 1102 20-21 0.7317007566429652 1102 22-23 0.80822929304437 1102 24-25 0.6301400570591937 1102 26-27 0.5970443752628825 1102 28-29 0.4783232394649062 1102 30-31 0.5123769545375723 1102 32-33 0.5306869974295338 1102 34-35 0.6363574984076052 1102 36-37 0.5440155609587691 1102 38-39 0.6762274705910656 1102 40-41 0.671602956957237 1102 42-43 0.6582663464792375 1102 44-45 0.8040423150583038 1102 46-47 0.7521866025126087 1102 48-49 0.7000509063234048 1102 50-51 0.8096809330351604 1102 52-53 0.809528391547353 1102 54-55 0.7584155759559934 1102 56-57 0.8099200242815243 1102 58-59 0.7567157772526372 1102 60-61 0.7457226043211946 1102 62-63 0.537490275819696 1102 64-65 0.5343910378458894 1102 66-67 0.46707364321083134 1102 68-69 0.5351259332485725 1102 70-71 0.7245971018751298 1102 72-73 0.6395639113410887 1102 74-75 0.5902299498978714 1102 76-77 0.4968930828980973 1102 78-79 0.6390897708478285 1102 80-81 0.7907229556454602 1102 82-83 0.666034721340246 1102 84-85 0.5875160518407512 1102 86-87 0.47793900205844153 1102 88-89 0.6527677939876995 1102 90-91 0.7516583158614267 1102 92-93 0.7073651443362508 1102 94-95 0.5565094905098533 1102 96-97 0.6076739441380745 1102 98-99 0.6680466654282817 1102 100-101 0.730637592345678 1103 1 0.5329099293257968 1103 2 0.4715150353451065 1103 3 0.554224911213403 1103 4 0.4928881643335643 1103 5 0.5445471604496888 1103 6 0.5100025695581536 1103 7 0.5154794858230147 1103 8 0.48353106348479 1103 9 0.4322471507561261 1103 10-11 0.42166526858439823 1103 12-13 0.4296804049124816 1103 14-15 0.6570634362557612 1103 16-17 0.640997796552476 1103 18-19 0.6627842773885533 1103 20-21 0.49050454891884954 1103 22-23 0.5841666157406564 1103 24-25 0.6757086823375715 1103 26-27 0.6294656111591266 1103 28-29 0.5766575472319246 1103 30-31 0.660178015255795 1103 32-33 0.7957747082490485 1103 34-35 0.7832748892199533 1103 36-37 0.6780530405074501 1103 38-39 0.5440868951722564 1103 40-41 0.6285071536230973 1103 42-43 0.5923545515880946 1103 44-45 0.6004617034597075 1103 46-47 0.6712558572437217 1103 48-49 0.7660113246697833 1103 50-51 0.9453259334830832 1103 52-53 0.9179654745643901 1103 54-55 0.9411137396199933 1103 56-57 0.9837292309510488 1103 58-59 0.8545426329594434 1103 60-61 1.009579405003926 1103 62-63 0.8868085455083623 1103 64-65 0.613460241497819 1103 66-67 0.6875751190063681 1103 68-69 0.6583656659394101 1103 70-71 0.8270702970903443 1103 72-73 0.9118121381075213 1103 74-75 0.5791679664852509 1103 76-77 0.6095025495213875 1103 78-79 0.7589518924100069 1103 80-81 0.5573082688865973 1103 82-83 0.799380765532387 1103 84-85 0.6727806695248368 1103 86-87 0.7897394136780918 1103 88-89 0.7667884213343932 1103 90-91 0.9028959252298208 1103 92-93 0.8955022589438002 1103 94-95 0.7622164696553995 1103 96-97 0.975691124818514 1103 98-99 1.2106980798502143 1103 100-101 1.0257134017150165 1104 1 0.4610101325593803 1104 2 0.4183509909244485 1104 3 0.42837511098456815 1104 4 0.5341039975791801 1104 5 0.3930662955401445 1104 6 0.39083662368656746 1104 7 0.4823932742755517 1104 8 0.3206173582044869 1104 9 0.4405755132489375 1104 10-11 0.41565677090699893 1104 12-13 0.5011791926957514 1104 14-15 0.5500928047798581 1104 16-17 0.6346266735063963 1104 18-19 0.5085858804219683 1104 20-21 0.550017438108739 1104 22-23 0.6469641583724624 1104 24-25 0.6569320400711831 1104 26-27 0.5409521607423144 1104 28-29 0.49882086816554505 1104 30-31 0.578873861638165 1104 32-33 0.5457452359453114 1104 34-35 0.5726715619221778 1104 36-37 0.37985241051490704 1104 38-39 0.5008268907871454 1104 40-41 0.49438403681048015 1104 42-43 0.3995664196717357 1104 44-45 0.5597115790262492 1104 46-47 0.5195275178089247 1104 48-49 0.5124149610043887 1104 50-51 0.39470254088396217 1104 52-53 0.40540841664847704 1104 54-55 0.5332383594736854 1104 56-57 0.7251536724114445 1104 58-59 0.4848354641998327 1104 60-61 0.40409287807032257 1104 62-63 0.44463919273129093 1104 64-65 0.49048614909160904 1104 66-67 0.596998792980127 1104 68-69 0.6572831273371875 1104 70-71 0.6271494035383647 1104 72-73 0.4467468432628827 1104 74-75 0.39960449818083177 1104 76-77 0.5475910789379057 1104 78-79 0.7366020001904516 1104 80-81 0.6566976491662011 1104 82-83 0.7507517428414054 1104 84-85 0.8155788081005433 1104 86-87 0.7817542758878773 1104 88-89 0.793581762283182 1104 90-91 0.7435331452127656 1104 92-93 0.9016328694261375 1104 94-95 1.0903253150038736 1104 96-97 1.0234694422256645 1104 98-99 0.9984294977715642 1104 100-101 0.7943294083227528 1105 1 0.38407466946521396 1105 2 0.35079502134182405 1105 3 0.40098439266156305 1105 4 0.40125759987650156 1105 5 0.31495970486454894 1105 6 0.3322441132477465 1105 7 0.30969240743691984 1105 8 0.3539200060577059 1105 9 0.4295489006641162 1105 10-11 0.4708715221399089 1105 12-13 0.5156450925396001 1105 14-15 0.5044690988326366 1105 16-17 0.5120174965579949 1105 18-19 0.4844176611537918 1105 20-21 0.5574631180015857 1105 22-23 0.5075476118818614 1105 24-25 0.48900457195842506 1105 26-27 0.4734158277723921 1105 28-29 0.5139045358449366 1105 30-31 0.43948716279886924 1105 32-33 0.567288432827759 1105 34-35 0.5318882810055072 1105 36-37 0.5552963812369782 1105 38-39 0.48558338108038157 1105 40-41 0.42585252030891496 1105 42-43 0.4509470568196008 1105 44-45 0.4553254404849767 1105 46-47 0.4035966219290543 1105 48-49 0.2740832980922434 1105 50-51 0.3142419130778933 1105 52-53 0.13872187772975764 1105 54-55 0.3211549567576597 1105 56-57 0.45317336453030777 1105 58-59 0.5020267949756914 1105 60-61 0.5273208701402794 1105 62-63 0.35820458369886765 1105 64-65 0.33184274055266627 1105 66-67 0.46559136625610975 1105 68-69 0.22938012906207206 1105 70-71 0.3497406195316799 1105 72-73 0.44891760534149583 1105 74-75 0.290116244017085 1105 76-77 0.30531363506981535 1105 78-79 0.3967072890895338 1105 80-81 0.41660013112931793 1105 82-83 0.45834804864473 1105 84-85 0.34188087261545874 1105 86-87 0.5026378920407986 1105 88-89 0.5527592605054181 1105 90-91 0.6666792395166858 1105 92-93 0.622822162183418 1105 94-95 0.7265701558111637 1105 96-97 0.8593169165383543 1105 98-99 0.8842524132273049 1105 100-101 0.6567398522777648 1106 1 0.3040424832805151 1106 2 0.2820149159869203 1106 3 0.04444842324145881 1106 4 0.2700093234175114 1106 5 0.3348521357769769 1106 6 -0.15902093062329214 1106 7 0.05372316849224035 1106 8 0.14736078770494032 1106 9 0.2590300569512962 1106 10-11 -0.03758515131021056 1106 12-13 0.07250986829307493 1106 14-15 0.3437998516350902 1106 16-17 -0.039105497340919726 1106 18-19 -0.33478011983120126 1106 20-21 0.0444769284714539 1106 22-23 0.1130962564992899 1106 24-25 0.16356153385634542 1106 26-27 -0.06918708649642014 1106 28-29 -0.009584182887259374 1106 30-31 0.13201751221723868 1106 32-33 0.2508050402146722 1106 34-35 0.0652311252658393 1106 36-37 0.057638384107370655 1106 38-39 0.04016654080155746 1106 40-41 0.04852485931257178 1106 42-43 -0.28822357021719824 1106 44-45 0.010460636550973845 1106 46-47 -0.24663677320013733 1106 48-49 0.10117615493316379 1106 50-51 -0.0849578606727519 1106 52-53 0.06028717502422154 1106 54-55 -0.16896198235428983 1106 56-57 -0.6104016469935161 1106 58-59 -0.856683352227293 1106 60-61 -0.1047846478076977 1106 62-63 -0.008493987347250709 1106 64-65 0.05425501357363771 1106 66-67 -0.562002399406726 1106 68-69 -0.7248763378650658 1106 70-71 -0.5060314563866548 1106 72-73 -0.004800915503036407 1106 74-75 0.05665770863945596 1106 76-77 0.0784968970460369 1106 78-79 0.08294558159130005 1106 80-81 -0.0014687682400413848 1106 82-83 -0.04565484388481522 1106 84-85 -0.1297559327597746 1106 86-87 -0.1092080933415609 1106 88-89 -0.0833544338204355 1106 90-91 0.07272763554162154 1106 92-93 0.05502661390112351 1106 94-95 -0.17770764779876203 1106 96-97 0.02503324534066209 1106 98-99 -0.08371262078054897 1106 100-101 -0.4524571638072761 1107 1 0.36752032419446934 1107 2 0.3030474380357262 1107 3 0.284994871744491 1107 4 0.3143271786171198 1107 5 0.3733990044836304 1107 6 0.34733773883332475 1107 7 0.30336230358263094 1107 8 0.2649363586003588 1107 9 0.35077277064907975 1107 10-11 0.4225696585476513 1107 12-13 0.26562836856545147 1107 14-15 0.4018625798593689 1107 16-17 0.4289807350329511 1107 18-19 0.4253473102434029 1107 20-21 0.45080812307611495 1107 22-23 0.5222776104866114 1107 24-25 0.47877833959266525 1107 26-27 0.4988281454093979 1107 28-29 0.45689800298280403 1107 30-31 0.5000115438047672 1107 32-33 0.4741508687430738 1107 34-35 0.4511521808578891 1107 36-37 0.5443531682079552 1107 38-39 0.6332385524835971 1107 40-41 0.5560516853207034 1107 42-43 0.654050007056064 1107 44-45 0.6907488246145945 1107 46-47 0.6134335998145488 1107 48-49 0.6812400759526369 1107 50-51 0.6523143428579061 1107 52-53 0.5505236995917215 1107 54-55 0.688947083174341 1107 56-57 0.5761158378818436 1107 58-59 0.6482355018907526 1107 60-61 0.6844251665770145 1107 62-63 0.9453912799526343 1107 64-65 0.7909797006629624 1107 66-67 0.7678254343612281 1107 68-69 0.7742169444860991 1107 70-71 0.7079268933856433 1107 72-73 0.7195756953171113 1107 74-75 0.7811999766552091 1107 76-77 0.8714685003446654 1107 78-79 0.7585941005953742 1107 80-81 0.8611266292310695 1107 82-83 0.8149440318328338 1107 84-85 0.7802617880443918 1107 86-87 0.7839114051520752 1107 88-89 1.0776484363033703 1107 90-91 1.0908791008829013 1107 92-93 1.0794557026391907 1107 94-95 1.1667607645192248 1107 96-97 1.230475658589686 1107 98-99 0.9220231902964109 1107 100-101 0.606455003178981 1108 1 0.39386142636020693 1108 2 0.28570998927027347 1108 3 0.3744651083083177 1108 4 0.40672981919325935 1108 5 0.4102080358740423 1108 6 0.37254458431714 1108 7 0.2964430809986567 1108 8 0.24526921187379713 1108 9 0.34478687755110826 1108 10-11 0.3158514840180615 1108 12-13 0.44524689544278573 1108 14-15 0.5360060702789937 1108 16-17 0.4722408890700862 1108 18-19 0.4424682712857333 1108 20-21 0.4963507647369738 1108 22-23 0.6429642285691841 1108 24-25 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-0.6903392617405544 2316 50-51 -0.7460090703902935 2316 52-53 -0.6691569100166888 2316 54-55 -0.6527836458862595 2316 56-57 -0.5616200394560948 2316 58-59 -0.5237415490971102 2316 60-61 -0.7385473537122564 2316 62-63 -0.6650635267174358 2316 64-65 -0.6693166615079917 2316 66-67 -0.5167787782382298 2316 68-69 -0.39279695839300643 2316 70-71 -0.6557215559223657 2316 72-73 -0.5058756865480198 2316 74-75 -0.4289685999309505 2316 76-77 -0.2946216471770384 2316 78-79 -0.39817240911653684 2316 80-81 -0.31540202258666383 2316 82-83 -0.22453150339865147 2316 84-85 -0.2884930447397309 2316 86-87 -0.47129579726565396 2316 88-89 -0.5937332789373997 2316 90-91 -0.4867280035143082 2316 92-93 -0.558495385288154 2316 94-95 -0.6686391290718596 2316 96-97 -0.7692949324225928 2316 98-99 -0.8157733035449191 2316 100-101 -0.7188741693246126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12019.0 3 4520.0 4 4797.0 5 5496.0 6 6317.0 7 7813.0 8 9798.0 9 12274.0 10 15380.0 11 18517.0 12 22095.0 13 25500.0 14 28710.0 15 32945.0 16 37233.0 17 41988.0 18 41876.0 19 40635.0 20 43669.0 21 47815.0 22 52222.0 23 57690.0 24 64470.0 25 72574.0 26 81462.0 27 92765.0 28 104901.0 29 119836.0 30 137691.0 31 161324.0 32 190156.0 33 227535.0 34 277503.0 35 342213.0 36 411118.0 37 417857.0 38 199935.0 39 10521.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.710713724533164 18.094263462734983 14.25687820988404 23.938144602847807 2 15.76938298017263 19.22222353148442 40.33949871288349 24.668894775459457 3 19.581939953120347 22.98373236983654 28.870272805548307 28.564054871494804 4 13.236972090667823 16.03471694902881 36.08499662866902 34.64331433163435 5 14.728203117657019 36.34373040973106 33.684785692338245 15.243280780273674 6 33.54384915986737 34.842443692932996 17.197574531139736 14.416132616059892 7 30.116822691758273 31.348787274536633 19.886180277589315 18.648209756115783 8 26.681731999905743 35.101642148143334 19.121689539574373 19.094936312376543 9 26.543385850485894 16.905515105674198 19.56803243449533 36.983066609344576 10-11 25.72035480731553 25.9392678919956 27.01380280407042 21.32657449661845 12-13 26.328436021885526 23.045679327565722 27.343584874510558 23.282299776038197 14-15 23.014287637539038 24.862178989366708 25.374640819099838 26.748892553994413 16-17 23.183067059555604 27.998537945108865 25.902293756654927 22.916101238680607 18-19 23.03297554056155 26.723343930804443 27.830330876172244 22.413349652461765 20-21 23.627165408077484 26.00222737952392 27.94402810809257 22.426579104306025 22-23 23.31488937215714 25.752087411328304 27.816823387168853 23.1161998293457 24-25 23.14843839717191 25.97057876198307 27.90702922131353 22.973953619531486 26-27 22.97653976433279 26.373878725528332 27.74078417683965 22.908797333299226 28-29 22.849116920092946 26.442377259645223 27.936523948147656 22.77198187211417 30-31 23.104609770338513 26.021748811871525 27.880734621297027 22.99290679649293 32-33 22.926366762376112 26.074417699671027 27.857628257532895 23.141587280419962 34-35 23.375387363385475 26.22000156633838 27.507847858247814 22.89676321202833 36-37 23.393016389777173 26.00888851336108 27.56539859930043 23.032696497561318 38-39 23.020640413558223 26.229230751769528 27.643847664906584 23.106281169765666 40-41 23.329818290482372 26.030764766053306 27.60300895330261 23.036407990161713 42-43 23.21742704503685 25.91760362040563 27.585465797088165 23.279503537469363 44-45 23.159870096479647 26.284391612288836 27.43294039577196 23.122797895459556 46-47 23.195918271778414 26.49091202389809 27.017632970536347 23.295536733787152 48-49 23.378920016909884 26.240643506407785 27.158793319188135 23.221643157494196 50-51 23.344384725779573 26.46506601747816 27.015806589092893 23.174742667649376 52-53 23.41141639622224 26.507288676641544 26.89419191233622 23.1871030148 54-55 23.192937920953533 26.315664728791297 27.08211473953599 23.40928261071918 56-57 23.167563689997124 26.62867227379865 27.133747952556035 23.070016083648184 58-59 23.309008346685214 26.267020954767933 27.0386002229958 23.38537047555105 60-61 23.290148045989163 26.302924074556355 27.361121864618426 23.045806014836057 62-63 23.124667305746055 26.411133540493108 27.12499881501237 23.339200338748466 64-65 23.01797470630837 26.245831953201243 27.105626715948272 23.630566624542116 66-67 23.01357655974018 26.450354657298192 27.037492999570674 23.49857578339095 68-69 22.961878825321914 26.214346013865352 27.21766863664446 23.606106524168272 70-71 22.985648811796782 26.267513021001687 27.08440183556832 23.66243633163321 72-73 23.237031222524994 26.21397146794252 26.916570222811163 23.632427086721318 74-75 23.067896833440624 26.429718345288443 26.81815955094306 23.684225270327868 76-77 23.123056259830925 26.336539083093317 26.864633086497786 23.675771570577968 78-79 23.28315160951619 26.310774300024566 26.87721828259861 23.528855807860637 80-81 23.0360634554562 26.566338064928324 26.834180728514234 23.56341775110124 82-83 23.28240349462999 26.57000952025827 26.632146872822105 23.51544011228964 84-85 23.34488267331838 26.190826390179623 26.66494206992402 23.799348866577976 86-87 23.236090570304714 26.122677165173364 26.856055774465972 23.78517649005595 88-89 23.37517713082474 26.153424639427513 26.741851694716186 23.729546535031567 90-91 23.263806071585186 26.33270323533959 26.73184033251702 23.671650360558203 92-93 23.39363469022999 25.864255359435557 26.835672134124415 23.906437816210037 94-95 23.29039379767265 26.00953693605132 26.748075493775445 23.951993772500586 96-97 23.395357825596257 25.980558586815732 26.684377687275006 23.939705900313008 98-99 23.476047485251005 25.8496491933271 26.453299858321166 24.221003463100725 100-101 24.111117614229308 25.363833884412685 26.425061285497737 24.09998721586027 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 365.0 1 262.0 2 209.5 3 281.0 4 264.5 5 245.5 6 449.0 7 1046.5 8 1746.5 9 2035.5 10 1894.5 11 1760.0 12 1865.5 13 2050.5 14 2258.5 15 2477.5 16 2670.0 17 2881.0 18 3134.5 19 3389.5 20 3765.0 21 4356.0 22 5161.5 23 6395.0 24 7984.5 25 9896.0 26 12825.0 27 16666.0 28 20385.0 29 24375.5 30 28960.0 31 34569.5 32 41051.5 33 47238.0 34 53197.5 35 58594.5 36 65614.0 37 73458.5 38 79380.0 39 84528.0 40 90479.0 41 100129.5 42 109494.5 43 116284.0 44 123483.5 45 131794.0 46 137901.5 47 141284.5 48 144874.5 49 145281.0 50 143011.0 51 139458.5 52 136065.5 53 134202.0 54 131853.5 55 124882.0 56 113563.5 57 103623.5 58 93216.5 59 79969.5 60 66451.5 61 55293.5 62 45771.0 63 36705.0 64 29262.5 65 23072.5 66 17629.5 67 14042.5 68 10970.0 69 7840.5 70 5845.5 71 4398.5 72 3542.5 73 2808.5 74 2119.0 75 1721.0 76 1239.0 77 791.0 78 495.5 79 300.5 80 182.0 81 104.0 82 69.5 83 45.5 84 29.5 85 21.5 86 15.0 87 8.5 88 9.5 89 7.5 90 4.5 91 5.0 92 3.5 93 1.5 94 1.5 95 2.5 96 1.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18258229273491383 2 0.048460718666425366 3 0.04659353033606518 4 0.05262598494184426 5 0.05983620449446594 6 0.06477707207634215 7 0.036797973095252456 8 0.03493078476489227 9 0.040733431576165484 10-11 0.042471353022116125 12-13 0.0383635386953237 14-15 0.03457171008597684 16-17 0.03264706980699018 18-19 0.04047489780734638 20-21 0.050126825176592935 22-23 0.04656480436175194 24-25 0.06769275846913538 26-27 0.05212328039136267 28-29 0.04476943096717483 30-31 0.05324359338957879 32-33 0.04927940893435253 34-35 0.049336860882979 36-37 0.046622256310378404 38-39 0.026844422995716957 40-41 0.025566117138778054 42-43 0.02259297879735836 44-45 0.003590746789154221 46-47 0.011188766995004554 48-49 0.010499343611486942 50-51 0.008948140998572319 52-53 0.009350304638957592 54-55 0.0109733221876553 56-57 0.008775785152692917 58-59 0.011116952059221469 60-61 0.011303670892257488 62-63 0.0034758428919012857 64-65 0.00640589227185113 66-67 0.003834917570816708 68-69 0.00963756438208993 70-71 0.023871284654297262 72-73 0.021084865145913588 74-75 0.020697064492684932 76-77 0.012783058569389027 78-79 0.004452526018551234 80-81 0.0059893656443092405 82-83 0.0049408675818762085 84-85 0.004682333813057105 86-87 0.008445436448090728 88-89 0.030004280170172676 90-91 0.0369990549154451 92-93 0.02267915672029806 94-95 0.03221618019229167 96-97 0.03526113346949445 98-99 0.021917918400997365 100-101 0.12080708497430462 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3481170.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.09913397331615 #Duplication Level Percentage of deduplicated Percentage of total 1 75.90056674260076 40.30254362115981 2 13.018644292716456 13.82557474899797 3 4.600668803435703 7.328745874814653 4 2.1177721262266838 4.498074634218611 5 1.1759514399076054 3.1221001526883985 6 0.7003872493626607 2.231397383226618 7 0.46272098831412906 1.7199058625530035 8 0.33488221191392636 1.4225564348558417 9 0.24794322396558205 1.1849013420411936 >10 1.2828315090554658 12.268929976122656 >50 0.09168756362578433 3.3933004755918086 >100 0.0615969184908674 6.284263854916762 >500 0.0034232076808912745 1.2339254525620236 >1k 8.150494443266815E-4 0.7909563973256416 >5k 1.0867325924355752E-4 0.3928237889249511 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7517 0.21593314891257825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6098 0.17517099136209952 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4032 0.11582312843095857 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.8725974313233768E-5 2 0.0 0.0 0.0 0.0 2.8725974313233768E-5 3 0.0 0.0 0.0 0.0 2.8725974313233768E-5 4 0.0 0.0 0.0 0.0 2.8725974313233768E-5 5 0.0 0.0 0.0 0.0 2.8725974313233768E-5 6 5.7451948626467536E-5 0.0 0.0 0.0 2.8725974313233768E-5 7 1.1490389725293507E-4 0.0 0.0 0.0 2.8725974313233768E-5 8 1.1490389725293507E-4 0.0 0.0 0.0 2.8725974313233768E-5 9 1.1490389725293507E-4 0.0 0.0 0.0 5.7451948626467536E-5 10-11 1.4362987156616884E-4 0.0 0.0 1.4362987156616884E-5 5.7451948626467536E-5 12-13 1.4362987156616884E-4 0.0 0.0 2.8725974313233768E-5 5.7451948626467536E-5 14-15 1.723558458794026E-4 1.4362987156616884E-5 0.0 4.308896146985065E-5 1.1490389725293507E-4 16-17 1.867188330360195E-4 2.8725974313233768E-5 0.0 5.7451948626467536E-5 1.5799285872278572E-4 18-19 2.2980779450587017E-4 2.8725974313233768E-5 0.0 8.61779229397013E-5 1.723558458794026E-4 20-21 2.8725974313233767E-4 2.8725974313233768E-5 0.0 1.4362987156616884E-4 1.723558458794026E-4 22-23 3.1598571744557145E-4 2.8725974313233768E-5 0.0 2.4417078166248706E-4 1.867188330360195E-4 24-25 3.1598571744557145E-4 2.8725974313233768E-5 0.0 3.73437666072039E-4 2.010818201926364E-4 26-27 3.1598571744557145E-4 2.8725974313233768E-5 0.0 6.606974092043768E-4 2.010818201926364E-4 28-29 3.590746789154221E-4 2.8725974313233768E-5 0.0 0.002614063662504273 2.010818201926364E-4 30-31 4.021636403852728E-4 2.8725974313233768E-5 0.0 0.008330532550837794 2.010818201926364E-4 32-33 4.021636403852728E-4 2.8725974313233768E-5 0.0 0.020395441762395973 2.010818201926364E-4 34-35 4.1652662754188967E-4 2.8725974313233768E-5 0.0 0.03959875559079275 2.010818201926364E-4 36-37 4.596155890117403E-4 2.8725974313233768E-5 0.0 0.07633927673741873 2.010818201926364E-4 38-39 4.883415633249741E-4 2.8725974313233768E-5 0.0 0.1337337734152598 2.1544480734925328E-4 40-41 4.883415633249741E-4 2.8725974313233768E-5 0.0 0.19438866817765293 2.2980779450587014E-4 42-43 5.601564991080585E-4 2.8725974313233768E-5 0.0 0.25701129218050256 2.2980779450587014E-4 44-45 6.606974092043767E-4 2.8725974313233768E-5 0.0 0.3360795364776785 2.2980779450587014E-4 46-47 7.181493578308442E-4 2.8725974313233768E-5 0.0 0.43106197054438594 2.2980779450587014E-4 48-49 7.181493578308442E-4 2.8725974313233768E-5 0.0 0.5338004176756665 2.2980779450587014E-4 50-51 7.181493578308442E-4 2.8725974313233768E-5 0.0 0.6436198174751593 2.2980779450587014E-4 52-53 7.181493578308442E-4 2.8725974313233768E-5 0.0 0.7567570673078304 2.2980779450587014E-4 54-55 7.181493578308442E-4 2.8725974313233768E-5 0.0 0.8821172192107826 2.2980779450587014E-4 56-57 7.181493578308442E-4 2.8725974313233768E-5 0.0 1.0296825492578645 2.2980779450587014E-4 58-59 7.325123449874612E-4 2.8725974313233768E-5 0.0 1.191280517756961 2.2980779450587014E-4 60-61 7.46875332144078E-4 2.8725974313233768E-5 0.0 1.3638948974051828 2.2980779450587014E-4 62-63 7.46875332144078E-4 5.7451948626467536E-5 0.0 1.5367534478350668 2.2980779450587014E-4 64-65 7.46875332144078E-4 5.7451948626467536E-5 0.0 1.7211742029260277 2.2980779450587014E-4 66-67 7.46875332144078E-4 5.7451948626467536E-5 0.0 1.9336458719338614 2.2980779450587014E-4 68-69 7.46875332144078E-4 5.7451948626467536E-5 0.0 2.1718847399006656 2.2980779450587014E-4 70-71 7.899642936139287E-4 5.7451948626467536E-5 0.0 2.4252191073690743 2.2980779450587014E-4 72-73 8.186902679271624E-4 5.7451948626467536E-5 0.0 2.685691879454321 3.1598571744557145E-4 74-75 8.330532550837793E-4 5.7451948626467536E-5 0.0 2.9522832840682875 3.1598571744557145E-4 76-77 8.330532550837793E-4 5.7451948626467536E-5 0.0 3.2454893038834642 3.1598571744557145E-4 78-79 8.330532550837793E-4 5.7451948626467536E-5 0.0 3.560124900536314 3.1598571744557145E-4 80-81 8.7614221655363E-4 8.61779229397013E-5 0.0 3.905612193601576 3.1598571744557145E-4 82-83 9.192311780234806E-4 8.61779229397013E-5 0.0 4.260765777023242 3.447116917588052E-4 84-85 9.766831266499482E-4 8.61779229397013E-5 0.0 4.6139803571787645 3.447116917588052E-4 86-87 0.0010628610495896495 1.005409100963182E-4 0.0 4.983856002435962 3.590746789154221E-4 88-89 0.0010915870239028831 1.1490389725293507E-4 0.0 5.3885618915479565 3.73437666072039E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5585 0.0 46.01967 1 GTATCAA 8955 0.0 42.307796 1 ATCAACG 11515 0.0 32.48273 3 TATCAAC 11630 0.0 32.2428 2 TCAACGC 11770 0.0 31.778986 4 CAACGCA 11915 0.0 31.389992 5 AACGCAG 12410 0.0 30.132301 6 ACGCAGA 14110 0.0 26.363794 7 CGCAGAG 14290 0.0 25.832605 8 GTACATG 11045 0.0 25.511084 1 TACATGG 11130 0.0 24.339699 2 GCAGAGT 15335 0.0 23.234093 9 ACATGGG 11440 0.0 23.015768 3 GAGTACT 8505 0.0 20.888838 12-13 GTACTTT 8775 0.0 20.219618 14-15 CATGGGG 7705 0.0 20.108824 4 CAGAGTA 15010 0.0 19.842983 10-11 GTGGTAT 2900 0.0 19.530882 1 AGTACTT 9000 0.0 19.52883 12-13 AGAGTAC 14650 0.0 19.406475 10-11 >>END_MODULE