FastQCFastQC Report
Thu 26 May 2016
SRR938646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481170
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75780.21768543334568552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64330.18479419275703285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42940.1233493337010258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55550.045.1213531
GTATCAA83350.039.544241
ATCAACG108450.029.8068683
TATCAAC111500.029.2612132
TCAACGC111300.029.0436174
CAACGCA113950.028.3264685
AACGCAG120650.026.8083366
GTACATG112250.024.236241
TACATGG111500.023.9720132
ACGCAGA137250.023.323717
ACATGGG111600.023.3002263
CGCAGAG138250.022.8801658
GCAGAGT152500.020.1192939
CATGGGG76450.019.7114584
GAGTACT82750.019.68684812-13
AGTACTT87900.018.85760912-13
GGACCGA7900.018.6373736
GTGGTAT27100.018.4278891
GTACTTT88600.018.27976814-15
CAGAGTA148750.018.10406710-11
Produced by FastQC (version 0.11.5)