FastQCFastQC Report
Thu 26 May 2016
SRR938645_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938645_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3474775
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76920.22136685109107784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61830.17793957882165032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.11776302062723486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51250.047.6090281
GTATCAA85200.042.229951
ATCAACG105850.033.4508633
TCAACGC107900.032.7712824
TATCAAC109500.032.422642
CAACGCA111000.031.6847765
AACGCAG115250.030.5523156
ACGCAGA135200.025.7916957
CGCAGAG134950.025.593058
GTACATG108550.025.5070061
TACATGG108200.025.1691912
ACATGGG113550.023.6903343
GCAGAGT146100.022.8256119
GTGGTAT25200.022.3148881
GAGTACT80200.021.64750712-13
CATGGGG78750.020.700724
GTACTTT83500.020.67954414-15
CAGAGTA142300.019.67781610-11
AGTACTT85650.018.93905312-13
AGAGTAC139550.018.73809810-11
Produced by FastQC (version 0.11.5)