FastQCFastQC Report
Thu 26 May 2016
SRR938645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938645_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3474775
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76860.22119417804030475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65230.18772438503212438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42600.12259786604888086No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54950.046.6544881
GTATCAA86250.041.9108921
ATCAACG111300.031.8637643
TCAACGC113550.031.2737964
TATCAAC115700.031.0374472
CAACGCA115750.030.7615345
AACGCAG121200.029.3124586
ACGCAGA140450.025.294917
CGCAGAG143250.024.7010218
ACCGTCG1601.8628652E-623.7477958
GTACATG103250.023.6319561
TACATGG103400.022.631692
GCAGAGT158150.022.1636079
ACATGGG106400.021.7144133
CCGTCGT2005.397378E-721.3730149
GAGTACT92100.021.0919612-13
GTGGTAT28200.021.0830441
GTACTTT95500.020.01777514-15
AGTACTT94700.019.4596512-13
CAGAGTA152750.019.27807810-11
Produced by FastQC (version 0.11.5)