##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938645_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3474775 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78846256232418 31.0 31.0 34.0 30.0 34.0 2 32.031218999791356 31.0 31.0 34.0 30.0 34.0 3 32.10012619522127 33.0 31.0 34.0 30.0 34.0 4 35.765919807757335 37.0 35.0 37.0 35.0 37.0 5 35.63155571224036 37.0 35.0 37.0 33.0 37.0 6 35.66616485959523 37.0 35.0 37.0 33.0 37.0 7 35.599936110971214 37.0 35.0 37.0 33.0 37.0 8 35.587045779942585 37.0 35.0 37.0 33.0 37.0 9 37.15949608248016 39.0 37.0 39.0 33.0 39.0 10-11 37.086128886042985 39.0 37.0 39.0 33.0 39.0 12-13 36.97037103121785 39.0 37.0 39.0 33.0 39.0 14-15 38.03291263463102 40.0 37.0 41.0 33.0 41.0 16-17 37.94322251081005 40.0 37.0 41.0 33.0 41.0 18-19 37.92254318624947 40.0 37.0 41.0 33.0 41.0 20-21 37.914995503305974 40.0 37.0 41.0 32.5 41.0 22-23 37.946443582678015 40.0 37.0 41.0 33.0 41.0 24-25 37.87492211725939 40.0 37.0 41.0 32.0 41.0 26-27 37.70294364383305 40.0 37.0 41.0 32.0 41.0 28-29 37.565271276557475 39.5 37.0 41.0 32.0 41.0 30-31 37.34357562719889 39.0 36.0 41.0 31.5 41.0 32-33 37.15134332438791 39.0 36.0 41.0 31.0 41.0 34-35 36.99075235087164 39.0 36.0 40.5 30.5 41.0 36-37 37.01694426257815 39.0 36.0 41.0 31.0 41.0 38-39 36.88139778834601 39.0 36.0 41.0 30.0 41.0 40-41 36.71283421228712 39.0 35.0 40.5 30.0 41.0 42-43 36.502993431229356 39.0 35.0 40.0 30.0 41.0 44-45 36.23758905252858 39.0 35.0 40.0 30.0 41.0 46-47 35.89713204452087 38.0 35.0 40.0 28.5 41.0 48-49 35.52483052859537 38.0 34.5 40.0 27.5 41.0 50-51 35.165824549791 38.0 34.0 40.0 26.5 41.0 52-53 34.64946737558547 38.0 33.0 40.0 25.5 41.0 54-55 34.31507320617882 38.0 33.0 40.0 24.0 41.0 56-57 33.94115949953594 37.0 33.0 40.0 24.0 41.0 58-59 33.46800238864387 37.0 32.0 39.5 23.0 41.0 60-61 33.047178018720636 36.0 31.0 39.0 22.0 40.0 62-63 32.54918750134901 36.0 31.0 39.0 20.5 40.0 64-65 32.03790374916362 35.0 30.5 38.5 18.5 40.0 66-67 31.52691728241397 35.0 30.0 38.0 17.5 40.0 68-69 30.943982272233455 34.5 29.0 37.5 13.0 39.5 70-71 30.363852911339585 34.0 29.0 37.0 9.0 39.0 72-73 29.754507414149117 34.0 28.0 36.0 7.5 39.0 74-75 29.110451755869086 33.5 27.5 36.0 6.0 38.5 76-77 27.834648574368124 31.5 26.0 34.5 2.0 36.5 78-79 28.446962752984007 33.0 27.0 35.0 2.0 37.0 80-81 28.12533703621098 33.0 26.0 35.0 2.0 36.5 82-83 27.616496176011395 33.0 26.0 35.0 2.0 36.0 84-85 27.012114021771193 32.0 25.5 35.0 2.0 36.0 86-87 26.338890287860366 32.0 24.5 34.5 2.0 35.0 88-89 25.582604341288288 31.0 21.5 34.0 2.0 35.0 90-91 25.02791029635012 31.0 19.5 34.0 2.0 35.0 92-93 24.22211423761251 31.0 15.5 34.0 2.0 35.0 94-95 23.411852134310855 30.5 4.5 34.0 2.0 35.0 96-97 22.41353325035434 30.0 2.0 34.0 2.0 35.0 98-99 21.220802498003465 29.5 2.0 34.0 2.0 35.0 100-101 18.09849026195941 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4306741184304279 1101 2 0.4282899613312452 1101 3 0.3827988250456258 1101 4 0.2456001395099392 1101 5 0.23675576269922516 1101 6 0.2509934079208591 1101 7 0.32395766520407676 1101 8 0.3769164665002265 1101 9 0.4851308649966697 1101 10-11 0.4450512962778248 1101 12-13 0.4935174466004142 1101 14-15 0.5850894941220446 1101 16-17 0.6478487631853724 1101 18-19 0.6631643422244764 1101 20-21 0.6936765225668395 1101 22-23 0.6236266053066473 1101 24-25 0.6591727376076335 1101 26-27 0.707971532415975 1101 28-29 0.666204432628092 1101 30-31 0.6824412778584943 1101 32-33 0.6747368365596671 1101 34-35 0.5200543797951127 1101 36-37 0.6564104593592361 1101 38-39 0.7792368557396046 1101 40-41 0.916687657815352 1101 42-43 0.8595948303090211 1101 44-45 0.9054327665168245 1101 46-47 1.0358089080973016 1101 48-49 1.1512074578577867 1101 50-51 1.2776505345893057 1101 52-53 1.265931931060166 1101 54-55 1.371256833512895 1101 56-57 1.3411882847015306 1101 58-59 1.4368555810725496 1101 60-61 1.4335743720865892 1101 62-63 1.568579445407785 1101 64-65 1.4955785475929844 1101 66-67 1.7074654183907505 1101 68-69 1.7157719568137004 1101 70-71 1.8630854481252577 1101 72-73 2.0646285477651958 1101 74-75 2.0418526077100907 1101 76-77 1.9595461654625623 1101 78-79 2.2855115317080674 1101 80-81 2.6201892503124924 1101 82-83 2.6661852619075823 1101 84-85 2.8577451894973045 1101 86-87 3.0270088817243312 1101 88-89 3.038475361902247 1101 90-91 3.2972979511408624 1101 92-93 3.5708218081781276 1101 94-95 3.703885596328046 1101 96-97 3.951004029448484 1101 98-99 4.404969317015606 1101 100-101 4.915920097261605 1102 1 0.3128601466749821 1102 2 0.3005952007395365 1102 3 0.3039801075207862 1102 4 0.20642946599849665 1102 5 0.12447674169492018 1102 6 0.12049337669869686 1102 7 0.21663413617896765 1102 8 0.27160183743600186 1102 9 0.3754736843912241 1102 10-11 0.35364716969330345 1102 12-13 0.354100302837125 1102 14-15 0.4790012947865918 1102 16-17 0.4354161167944923 1102 18-19 0.4482514849360726 1102 20-21 0.5181382072163814 1102 22-23 0.45575261304317394 1102 24-25 0.48521863912305463 1102 26-27 0.4532325415164209 1102 28-29 0.40914308764286744 1102 30-31 0.4204292680054422 1102 32-33 0.33514277402988313 1102 34-35 0.4678326350346893 1102 36-37 0.6257499831718931 1102 38-39 0.5813752044484843 1102 40-41 0.5715112977613117 1102 42-43 0.5388007232465668 1102 44-45 0.48694439595242045 1102 46-47 0.5638701388751315 1102 48-49 0.649099242648326 1102 50-51 0.7053645420037427 1102 52-53 0.7162020028054172 1102 54-55 0.7094807153484979 1102 56-57 0.8466529855767391 1102 58-59 0.9958604443358894 1102 60-61 1.0646674190743113 1102 62-63 1.153016509735302 1102 64-65 1.1466183484612955 1102 66-67 1.0766543979517245 1102 68-69 1.2164102652624216 1102 70-71 1.2205796720243463 1102 72-73 1.4233721718700245 1102 74-75 1.5241707961013589 1102 76-77 1.5335645775031423 1102 78-79 1.9373414382716234 1102 80-81 2.174204934521633 1102 82-83 2.172390873023989 1102 84-85 2.31930659364032 1102 86-87 2.52440840386085 1102 88-89 2.607133523541048 1102 90-91 2.681504690363468 1102 92-93 2.9294496843148607 1102 94-95 3.185765750039703 1102 96-97 3.407792652912434 1102 98-99 3.761502329527481 1102 100-101 4.035151699188834 1103 1 0.30563955318251246 1103 2 0.33722747284876675 1103 3 0.2919484518831723 1103 4 0.27247966105579735 1103 5 0.18535233744315605 1103 6 0.19440616056209592 1103 7 0.2343591584634197 1103 8 0.21706004256540723 1103 9 0.3256884113344185 1103 10-11 0.3809491424336713 1103 12-13 0.3758774570924075 1103 14-15 0.573102489639389 1103 16-17 0.5008333415482653 1103 18-19 0.5329652057613856 1103 20-21 0.5743499216599872 1103 22-23 0.4969266605094518 1103 24-25 0.4142035176942329 1103 26-27 0.4358710563958752 1103 28-29 0.4690483728448953 1103 30-31 0.5547935697096733 1103 32-33 0.6330459666003847 1103 34-35 0.6808996593454353 1103 36-37 0.6963671748307121 1103 38-39 0.8870722006129057 1103 40-41 0.7658047887484045 1103 42-43 0.8796339375473323 1103 44-45 0.9652556867962048 1103 46-47 1.055863101123471 1103 48-49 1.1902570450964092 1103 50-51 1.2132473761027853 1103 52-53 1.3305081951526745 1103 54-55 1.2954450098717487 1103 56-57 1.4374861510936654 1103 58-59 1.5424792072245737 1103 60-61 1.4156375735122353 1103 62-63 1.7176422167106935 1103 64-65 1.706970054568714 1103 66-67 1.694503272645175 1103 68-69 1.7218425895898655 1103 70-71 1.509154223876493 1103 72-73 1.5089006315808184 1103 74-75 1.6489273647476352 1103 76-77 1.6125874196573733 1103 78-79 2.009775583992461 1103 80-81 2.2057778835319866 1103 82-83 2.2428213689331713 1103 84-85 2.4083831507033615 1103 86-87 2.5639779508814478 1103 88-89 2.7944032638236997 1103 90-91 2.9803901455205803 1103 92-93 3.0689930246574555 1103 94-95 3.071322816593348 1103 96-97 3.396182733557975 1103 98-99 3.905955226584993 1103 100-101 4.000833781158175 1104 1 0.1976223981481091 1104 2 0.2598969725593818 1104 3 0.20736916849843823 1104 4 0.1407090448938746 1104 5 0.14299343363529715 1104 6 0.1577343839525227 1104 7 0.18800874596749395 1104 8 0.20848084966827685 1104 9 0.19082027048320782 1104 10-11 0.20218017785668252 1104 12-13 0.27543170324642574 1104 14-15 0.4498561873193765 1104 16-17 0.44235084098482247 1104 18-19 0.4108363601662859 1104 20-21 0.4253359712086535 1104 22-23 0.3368135025973302 1104 24-25 0.34753770749458823 1104 26-27 0.3172065733477396 1104 28-29 0.4474347293997454 1104 30-31 0.48581185407031313 1104 32-33 0.4156334428369135 1104 34-35 0.4233572802867158 1104 36-37 0.5445304207960291 1104 38-39 0.6508551521341701 1104 40-41 0.724282429792467 1104 42-43 0.6638210055842322 1104 44-45 0.6526506241265579 1104 46-47 0.6988671666420814 1104 48-49 0.9466805244694783 1104 50-51 0.8339047275734899 1104 52-53 0.9871305517533386 1104 54-55 1.011407311544943 1104 56-57 1.0482581043681805 1104 58-59 1.2152496909133959 1104 60-61 1.058213870992276 1104 62-63 1.2525936582889798 1104 64-65 1.2291872745270425 1104 66-67 1.4118390867538473 1104 68-69 1.3799271774379172 1104 70-71 1.3820419504527486 1104 72-73 1.6062384341824867 1104 74-75 1.6465817156978453 1104 76-77 1.6371204054140485 1104 78-79 1.9049669685300472 1104 80-81 2.0956176525167614 1104 82-83 2.2696682586033674 1104 84-85 2.3673551221684868 1104 86-87 2.5920977646594494 1104 88-89 2.6790172341227247 1104 90-91 2.9695707793489987 1104 92-93 3.036595297758165 1104 94-95 3.479871269772019 1104 96-97 3.7167659261560893 1104 98-99 4.276405047718374 1104 100-101 4.633098401911315 1105 1 0.2944272235912315 1105 2 0.2882742994732652 1105 3 0.27252200700347373 1105 4 0.1574489226336624 1105 5 0.13890344072763838 1105 6 0.20849024617123746 1105 7 0.31369279101426173 1105 8 0.21643433941570578 1105 9 0.31240946787417556 1105 10-11 0.26921414299871316 1105 12-13 0.36272003071066194 1105 14-15 0.535242645131838 1105 16-17 0.4868360015644413 1105 18-19 0.3798468698133348 1105 20-21 0.4164340402105111 1105 22-23 0.3789656036219853 1105 24-25 0.21806854772349737 1105 26-27 0.433349714272687 1105 28-29 0.4919136229408636 1105 30-31 0.6021119357624869 1105 32-33 0.5799694478999768 1105 34-35 0.6220850273349185 1105 36-37 0.704902788805363 1105 38-39 0.6129649462354791 1105 40-41 0.6092970138972262 1105 42-43 0.607073280806091 1105 44-45 0.6910246277446674 1105 46-47 0.853224634105338 1105 48-49 0.8647798640913251 1105 50-51 0.8992849448278761 1105 52-53 1.0221838635080474 1105 54-55 1.061247788934395 1105 56-57 0.9765558134125314 1105 58-59 1.223366300198407 1105 60-61 1.3366774138197641 1105 62-63 1.4625672087384132 1105 64-65 1.5604321246322783 1105 66-67 1.6436606368043094 1105 68-69 1.7466621783625236 1105 70-71 1.6554497144307838 1105 72-73 1.6995615225261034 1105 74-75 1.948873377491374 1105 76-77 1.8554583338400548 1105 78-79 2.211584431588726 1105 80-81 2.4834633492018092 1105 82-83 2.5065379523084808 1105 84-85 2.7091538416624665 1105 86-87 2.9991695438760964 1105 88-89 3.1544576811479352 1105 90-91 3.2655668920197556 1105 92-93 3.6247166117354688 1105 94-95 3.559696569453415 1105 96-97 4.009378500042043 1105 98-99 4.272078452039892 1105 100-101 4.718500836258574 1106 1 0.18610412901763596 1106 2 0.10576628369137353 1106 3 0.10877282656819176 1106 4 0.12805927833438346 1106 5 -0.030514922061485095 1106 6 0.04763946626253812 1106 7 0.0919259229698568 1106 8 0.11029780710698134 1106 9 0.21923231470476168 1106 10-11 0.2146797606796369 1106 12-13 0.10309850152173539 1106 14-15 0.0616041717769491 1106 16-17 0.09158611515793069 1106 18-19 0.23464565409533122 1106 20-21 0.34833057827493263 1106 22-23 0.23286814490428043 1106 24-25 0.12375635898143145 1106 26-27 0.07913219462347598 1106 28-29 0.228944972174574 1106 30-31 0.2630102943205017 1106 32-33 0.23932875920437624 1106 34-35 0.3242277795563595 1106 36-37 0.38089698343929257 1106 38-39 0.33724153449944083 1106 40-41 0.23434800784561816 1106 42-43 0.47041489507985546 1106 44-45 -0.014140375096772573 1106 46-47 -0.054582375587266085 1106 48-49 0.48612974445949675 1106 50-51 0.3740853907224775 1106 52-53 0.5766694680759414 1106 54-55 0.4832177762710259 1106 56-57 0.43502958798394786 1106 58-59 0.9110498966132639 1106 60-61 0.8011530786896301 1106 62-63 1.028459441170618 1106 64-65 0.7585852507979212 1106 66-67 0.8309489150267773 1106 68-69 0.66036487218771 1106 70-71 0.909006357647872 1106 72-73 1.1151065507285516 1106 74-75 1.5030334687258957 1106 76-77 1.5685872574191535 1106 78-79 1.8439356005631602 1106 80-81 1.6597405747027665 1106 82-83 1.8664466235708481 1106 84-85 1.9494984806707052 1106 86-87 2.290997236362994 1106 88-89 2.5442159807529556 1106 90-91 2.516910592730781 1106 92-93 2.3270191871065187 1106 94-95 2.9255787379948934 1106 96-97 3.3938563404253124 1106 98-99 3.7589969358098365 1106 100-101 4.043026152510986 1107 1 0.20561109380436804 1107 2 0.18697550831770116 1107 3 0.178427849586825 1107 4 0.16752970994755145 1107 5 0.10969365399692776 1107 6 0.09352548354163304 1107 7 0.22022381274973668 1107 8 0.21025272610754797 1107 9 0.24776157799796295 1107 10-11 0.18446723759432615 1107 12-13 0.2771225027629285 1107 14-15 0.4019072113942386 1107 16-17 0.4534737567841489 1107 18-19 0.46625115892121016 1107 20-21 0.4484205613809351 1107 22-23 0.3496115891316762 1107 24-25 0.32685902786767684 1107 26-27 0.4353270183749842 1107 28-29 0.3761881844917667 1107 30-31 0.46534238223430435 1107 32-33 0.5028844465985429 1107 34-35 0.4755403570901535 1107 36-37 0.49886169666103086 1107 38-39 0.5200029067182186 1107 40-41 0.5884644218830459 1107 42-43 0.5886026551656727 1107 44-45 0.5874089671304574 1107 46-47 0.611136321578357 1107 48-49 0.7166479059873936 1107 50-51 0.5867678533029661 1107 52-53 0.7749710999690933 1107 54-55 0.8087406475598939 1107 56-57 0.8023269088845524 1107 58-59 0.92640768557564 1107 60-61 0.9468980633471276 1107 62-63 1.1014330892773074 1107 64-65 1.1482536924069748 1107 66-67 1.309143746259263 1107 68-69 1.2732756183140275 1107 70-71 1.458169326111335 1107 72-73 1.5179863923010402 1107 74-75 1.4973876915405242 1107 76-77 1.4658058313113322 1107 78-79 1.639453789738127 1107 80-81 1.949063216423312 1107 82-83 1.9784078145549095 1107 84-85 2.147567023305424 1107 86-87 2.50555856180943 1107 88-89 2.7520436165114894 1107 90-91 2.8704749190235432 1107 92-93 3.1074776878672985 1107 94-95 3.1873350478711444 1107 96-97 3.572845756798756 1107 98-99 3.8364551838438032 1107 100-101 4.216315839240323 1108 1 0.23952171120260246 1108 2 0.17108596187966896 1108 3 0.18006070206438807 1108 4 0.08198128090224799 1108 5 0.0439819029692714 1108 6 0.019507123594088682 1108 7 0.13024519228049058 1108 8 0.10671077704373033 1108 9 0.251878147868986 1108 10-11 0.2021964981637865 1108 12-13 0.28327623518083556 1108 14-15 0.41629913456016965 1108 16-17 0.36495855050106485 1108 18-19 0.366039659525498 1108 20-21 0.285218425631939 1108 22-23 0.3167538768883986 1108 24-25 0.36888110168215604 1108 26-27 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1.7323440013506683 2316 84-85 1.8874228256425987 2316 86-87 1.9066937458336035 2316 88-89 2.179494274462577 2316 90-91 2.431157673887519 2316 92-93 2.3551003021308468 2316 94-95 2.949657070475471 2316 96-97 3.0613309704572806 2316 98-99 3.31482767563012 2316 100-101 3.60849434080426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 21.0 4 59.0 5 121.0 6 211.0 7 589.0 8 1360.0 9 2514.0 10 4092.0 11 6300.0 12 8991.0 13 12916.0 14 17212.0 15 22230.0 16 27814.0 17 34386.0 18 34311.0 19 31864.0 20 33479.0 21 36056.0 22 39781.0 23 44842.0 24 51585.0 25 60158.0 26 70654.0 27 83384.0 28 99462.0 29 116965.0 30 137989.0 31 161234.0 32 192224.0 33 231988.0 34 286338.0 35 360711.0 36 457103.0 37 514239.0 38 277005.0 39 14579.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.166828691592606 18.96241309506748 14.923675611708848 23.94708260163107 2 16.095655543763858 19.973546420733985 39.26617517751365 24.6646228579885 3 19.553748367515972 23.258630940903647 29.367445174725436 27.82017551685495 4 13.677288457526027 16.780885093279423 35.70305991035391 33.838766538840645 5 14.550558641917515 36.53130684903489 33.81237639948551 15.105758109562089 6 33.45108676101331 35.346749070083675 16.87481923289997 14.327344936003051 7 29.925362073803342 31.47084343590592 19.845428840716306 18.758365649574433 8 26.533746789360464 35.531221445992905 18.94188832370441 18.99314344094222 9 26.20653135814549 16.84647207373139 19.519882582325472 37.42711398579765 10-11 25.61755797137944 26.133979897979003 26.951299004971546 21.297163125670007 12-13 26.1699390608025 23.1227777338101 27.418724953414248 23.28855825197315 14-15 22.81072875222137 24.962076681223962 25.355728068723877 26.87146649783079 16-17 22.93069335424596 28.17402565633746 25.976171118993317 22.919109870423267 18-19 22.85717780287932 26.8015195228468 27.92970767891446 22.411594995359412 20-21 23.45207963105525 26.05849299594938 28.024519573209776 22.464907799785596 22-23 23.132058910289153 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27.09429958200129 23.283343955134892 48-49 23.282255685619933 26.27925549136275 27.24777863314891 23.19071018986841 50-51 23.235792245541077 26.446590066982758 27.097351627083764 23.2202660603924 52-53 23.389946111618737 26.50292464979747 26.931340878186354 23.175788360397437 54-55 23.129886107733594 26.333604334093575 27.131886237238383 23.404623320934448 56-57 23.126950665870453 26.67424509500615 27.077839572346413 23.120964666776985 58-59 23.29863084660158 26.349864379708038 27.002568511630248 23.348936262060132 60-61 23.21813642609953 26.37739134188545 27.30627162909829 23.098200602916734 62-63 23.065119324272796 26.442100567662653 27.063766718708404 23.429013389356147 64-65 22.901151873142865 26.289961220510683 27.085897648049155 23.7229892582973 66-67 22.939634343670097 26.417163978403725 27.037982850929076 23.605218826997103 68-69 22.88251842819547 26.250323582521496 27.169477811556185 23.697680177726845 70-71 22.859494499643862 26.321056759070732 27.03285824262003 23.78659049866538 72-73 23.11674415612989 26.308606877541802 26.81432329330243 23.760325673025882 74-75 22.94279485722097 26.47413141858106 26.651696872459368 23.9313768517386 76-77 22.899051017691793 26.3776791303034 26.889539610478373 23.83373024152643 78-79 22.98337302415264 26.335201559813225 26.909411400738176 23.77201401529595 80-81 22.723153426137475 26.573577612120275 26.913959559372657 23.789309402369593 82-83 22.964963431806858 26.594816707202064 26.69578826162871 23.744431599362372 84-85 22.94868051627286 26.261272585282686 26.729278509932914 24.060768388511544 86-87 22.83558065358153 26.149405522326763 26.954964283566124 24.060049540525583 88-89 22.868267528804395 26.278532974883113 26.74558500000072 24.107614496311776 90-91 22.796737602327653 26.359991884296548 26.839250074465898 24.004020438909908 92-93 22.797263011516165 26.0202839441367 26.886989289644664 24.295463754702475 94-95 22.622356843248845 26.116295299695665 26.818340755876278 24.443007101179212 96-97 22.606701153312084 26.105992474332872 26.8201394334885 24.467166938866544 98-99 22.65333726644171 26.008676820801348 26.599966904331936 24.738019008425006 100-101 22.704009428023188 25.810983388001574 26.710919282624136 24.774087901351106 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 394.0 1 281.0 2 205.5 3 268.5 4 244.0 5 237.0 6 484.0 7 1094.0 8 1759.5 9 2098.0 10 1965.0 11 1796.0 12 1944.5 13 2136.5 14 2316.0 15 2535.5 16 2761.0 17 2969.0 18 3186.0 19 3442.0 20 3892.5 21 4584.5 22 5491.0 23 6658.0 24 8141.0 25 10108.0 26 13163.5 27 16938.0 28 20519.5 29 24777.5 30 29521.0 31 35050.0 32 41435.0 33 47471.0 34 52919.0 35 58252.5 36 65890.0 37 73507.0 38 79253.0 39 84426.0 40 90570.0 41 100549.5 42 109810.0 43 116456.5 44 123896.0 45 132607.0 46 138385.0 47 142022.5 48 146018.0 49 145475.5 50 143017.0 51 139630.5 52 135785.5 53 134216.0 54 132774.5 55 125764.5 56 113280.0 57 102923.0 58 92630.5 59 78917.0 60 64917.0 61 53484.0 62 44156.0 63 35021.0 64 27868.5 65 22026.0 66 16543.5 67 13461.5 68 10347.5 69 7082.5 70 5198.5 71 3877.0 72 3127.5 73 2541.0 74 2034.5 75 1726.5 76 1175.0 77 646.5 78 374.5 79 223.0 80 133.0 81 70.5 82 36.5 83 25.0 84 13.0 85 5.0 86 5.5 87 5.5 88 2.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.8778841795511943E-5 2 0.04377261837097366 3 2.590095761596075E-4 4 0.0 5 0.09813585052269572 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001007259462842918 26-27 0.011252527142045169 28-29 0.0 30-31 0.0 32-33 5.18019152319215E-4 34-35 0.014619651632120065 36-37 0.0032663985437906048 38-39 0.011856882819750919 40-41 0.00728104697426452 42-43 0.00571260009640912 44-45 0.0028059370750624143 46-47 1.0072594628429178E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.7339899705736343E-4 68-69 1.2950478807980374E-4 70-71 0.0 72-73 4.316826269326791E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.7557683591023886E-5 82-83 9.78480621047406E-4 84-85 0.004014648430473915 86-87 0.0012662690390025254 88-89 4.316826269326791E-5 90-91 8.633652538653581E-4 92-93 2.0145189256858356E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.914181493765784E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3474775.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.0691972703348 #Duplication Level Percentage of deduplicated Percentage of total 1 73.34536661276967 36.72343629799792 2 14.290879321011376 14.31065711780534 3 5.043363544807245 7.57551492692907 4 2.3757029491343338 4.757981584636925 5 1.3293744019786535 3.3280354589401284 6 0.7942673782193601 2.386099802727406 7 0.5262233757357972 1.8443307405584 8 0.3713998266832422 1.4876552950697126 9 0.2710052135658008 1.2212112149383765 >10 1.4705349897545115 13.193237700161639 >50 0.10688023804209026 3.730593367694334 >100 0.06958904031155672 6.711138526293431 >500 0.00431896914151845 1.4421677786415867 >1k 9.78966334840462E-4 0.8782524371369282 >5k 1.1517250998123082E-4 0.40968775046876604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7686 0.22119417804030475 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6523 0.18772438503212438 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4260 0.12259786604888086 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.877884179551194E-5 0.0 0.0 0.0 0.0 3 2.877884179551194E-5 0.0 0.0 0.0 0.0 4 2.877884179551194E-5 0.0 0.0 0.0 0.0 5 2.877884179551194E-5 0.0 0.0 0.0 0.0 6 2.877884179551194E-5 0.0 0.0 0.0 0.0 7 5.755768359102388E-5 0.0 0.0 0.0 0.0 8 5.755768359102388E-5 0.0 0.0 0.0 0.0 9 5.755768359102388E-5 0.0 0.0 0.0 2.877884179551194E-5 10-11 5.755768359102388E-5 0.0 0.0 2.877884179551194E-5 2.877884179551194E-5 12-13 8.633652538653581E-5 0.0 0.0 2.877884179551194E-5 2.877884179551194E-5 14-15 8.633652538653581E-5 0.0 0.0 2.877884179551194E-5 4.316826269326791E-5 16-17 8.633652538653581E-5 0.0 0.0 2.877884179551194E-5 1.1511536718204776E-4 18-19 1.2950478807980374E-4 0.0 0.0 2.877884179551194E-5 1.1511536718204776E-4 20-21 1.438942089775597E-4 0.0 0.0 5.755768359102388E-5 1.1511536718204776E-4 22-23 1.438942089775597E-4 0.0 0.0 2.0145189256858358E-4 1.1511536718204776E-4 24-25 1.7267305077307162E-4 0.0 0.0 4.0290378513716717E-4 1.438942089775597E-4 26-27 1.7267305077307162E-4 0.0 0.0 8.058075702743342E-4 1.7267305077307162E-4 28-29 2.446201552618515E-4 0.0 0.0 0.0025900957615960746 1.7267305077307162E-4 30-31 2.877884179551194E-4 0.0 0.0 0.008576094855062558 1.870624716708276E-4 32-33 2.877884179551194E-4 0.0 0.0 0.02164168903022498 2.0145189256858358E-4 34-35 3.309566806483873E-4 0.0 0.0 0.04365750300379161 2.0145189256858358E-4 36-37 3.8851436423941116E-4 0.0 0.0 0.07996201192882993 2.0145189256858358E-4 38-39 4.316826269326791E-4 0.0 0.0 0.13327481635501578 2.0145189256858358E-4 40-41 4.316826269326791E-4 0.0 0.0 0.19247289392838385 2.0145189256858358E-4 42-43 4.74850889625947E-4 0.0 0.0 0.25613169198005625 2.0145189256858358E-4 44-45 5.467979941147269E-4 0.0 0.0 0.3340216272996093 2.0145189256858358E-4 46-47 5.755768359102388E-4 0.0 0.0 0.4314811750401105 2.1584131346633956E-4 48-49 5.755768359102388E-4 0.0 0.0 0.5360922649667964 2.3023073436409552E-4 50-51 5.755768359102388E-4 0.0 0.0 0.6459986617838565 2.3023073436409552E-4 52-53 5.755768359102388E-4 0.0 0.0 0.7550129145052558 2.590095761596075E-4 54-55 5.899662568079947E-4 0.0 0.0 0.8792943427991741 3.0217783885287534E-4 56-57 6.619133612967746E-4 0.0 0.0 1.0282248490909482 3.1656725975063134E-4 58-59 6.619133612967746E-4 1.438942089775597E-5 0.0 1.1897317092473614 3.1656725975063134E-4 60-61 6.619133612967746E-4 2.877884179551194E-5 0.0 1.3658582210358943 3.1656725975063134E-4 62-63 6.619133612967746E-4 2.877884179551194E-5 0.0 1.545078458317445 3.1656725975063134E-4 64-65 6.619133612967746E-4 2.877884179551194E-5 0.0 1.7320977617255795 3.1656725975063134E-4 66-67 7.194710448877985E-4 2.877884179551194E-5 0.0 1.9447014554899238 3.1656725975063134E-4 68-69 7.194710448877985E-4 2.877884179551194E-5 0.0 2.181421818678907 3.1656725975063134E-4 70-71 7.338604657855545E-4 2.877884179551194E-5 0.0 2.437236943399213 3.4534610154614325E-4 72-73 7.770287284788223E-4 2.877884179551194E-5 0.0 2.702621033016526 4.6046146872819103E-4 74-75 8.058075702743343E-4 2.877884179551194E-5 0.0 2.9691131080429667 4.6046146872819103E-4 76-77 8.058075702743343E-4 2.877884179551194E-5 0.0 3.261319078213697 4.892403105237029E-4 78-79 8.058075702743343E-4 2.877884179551194E-5 0.0 3.586966062550813 4.892403105237029E-4 80-81 9.49701779251894E-4 2.877884179551194E-5 0.0 3.940729975322143 5.03629731421459E-4 82-83 0.0010072594628429178 2.877884179551194E-5 0.0 4.3046815980890845 5.18019152319215E-4 84-85 0.0010072594628429178 2.877884179551194E-5 0.0 4.673928527746401 5.18019152319215E-4 86-87 0.0010072594628429178 2.877884179551194E-5 0.0 5.058816757919578 5.18019152319215E-4 88-89 0.0010072594628429178 2.877884179551194E-5 0.0 5.477246728205424 5.18019152319215E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5495 0.0 46.654488 1 GTATCAA 8625 0.0 41.910892 1 ATCAACG 11130 0.0 31.863764 3 TCAACGC 11355 0.0 31.273796 4 TATCAAC 11570 0.0 31.037447 2 CAACGCA 11575 0.0 30.761534 5 AACGCAG 12120 0.0 29.312458 6 ACGCAGA 14045 0.0 25.29491 7 CGCAGAG 14325 0.0 24.701021 8 ACCGTCG 160 1.8628652E-6 23.747795 8 GTACATG 10325 0.0 23.631956 1 TACATGG 10340 0.0 22.63169 2 GCAGAGT 15815 0.0 22.163607 9 ACATGGG 10640 0.0 21.714413 3 CCGTCGT 200 5.397378E-7 21.373014 9 GAGTACT 9210 0.0 21.09196 12-13 GTGGTAT 2820 0.0 21.083044 1 GTACTTT 9550 0.0 20.017775 14-15 AGTACTT 9470 0.0 19.45965 12-13 CAGAGTA 15275 0.0 19.278078 10-11 >>END_MODULE