FastQCFastQC Report
Thu 26 May 2016
SRR938644_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938644_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3467952
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70630.20366487194747795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57840.16678431535384575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37130.10706607242545456No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA87100.043.340811
GGTATCA53000.042.7716871
ATCAACG110900.033.94093
TCAACGC112700.033.483154
TATCAAC112800.033.453472
CAACGCA116350.032.2693675
AACGCAG121350.031.011836
ACGCAGA137250.027.2044167
CGCAGAG140000.026.4325248
GTACATG111450.023.9370371
GCAGAGT151950.023.9146989
TACATGG113400.022.9247172
ACATGGG114450.022.257623
GTGGTAT28800.021.8735921
GAGTACT83400.021.27284412-13
TGACGCG4250.020.69264494-95
GGACCGA8000.020.1943996
GTACTTT87950.020.14691214-15
AGTACTT87450.019.90742912-13
AGAGTAC145950.019.67345810-11
Produced by FastQC (version 0.11.5)