##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938644_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3467952 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.998747387507095 31.0 31.0 34.0 28.0 34.0 2 31.178888865820518 31.0 31.0 34.0 27.0 34.0 3 31.23931069403498 31.0 31.0 34.0 27.0 34.0 4 34.90275269092537 37.0 35.0 37.0 32.0 37.0 5 34.88114166516722 37.0 35.0 37.0 32.0 37.0 6 34.846993845358874 37.0 35.0 37.0 32.0 37.0 7 34.75689801934975 37.0 35.0 37.0 31.0 37.0 8 34.716543077874206 37.0 35.0 37.0 31.0 37.0 9 36.18649248893872 39.0 35.0 39.0 32.0 39.0 10-11 36.00545912976881 38.0 35.0 39.0 30.0 39.0 12-13 35.813846616100804 38.0 35.0 39.0 30.0 39.0 14-15 36.97055654172837 39.0 36.0 41.0 31.0 41.0 16-17 36.88911135448242 39.0 36.0 41.0 31.0 41.0 18-19 36.85452163121058 39.0 36.0 41.0 30.0 41.0 20-21 36.77691660668891 39.0 36.0 41.0 30.0 41.0 22-23 36.613847308151904 39.0 36.0 41.0 30.0 41.0 24-25 36.47136133948797 39.0 36.0 41.0 29.5 41.0 26-27 36.253220200279586 39.0 35.5 40.5 29.0 41.0 28-29 36.0039083585932 39.0 35.0 40.0 27.5 41.0 30-31 35.74214204810217 38.0 35.0 40.0 27.0 41.0 32-33 35.46222713001795 38.0 34.0 40.0 26.5 41.0 34-35 35.156930516916034 38.0 34.0 40.0 25.5 41.0 36-37 34.94842344992088 38.0 34.0 40.0 25.0 41.0 38-39 34.714938096029016 38.0 33.5 40.0 24.5 41.0 40-41 34.431695277212604 38.0 33.0 40.0 24.0 41.0 42-43 34.02595292553069 38.0 33.0 40.0 23.5 41.0 44-45 33.57023453611815 38.0 32.5 40.0 21.0 41.0 46-47 33.08666397343447 37.0 31.5 40.0 18.0 41.0 48-49 32.60762029001555 37.0 31.0 40.0 15.0 41.0 50-51 31.887579470534774 36.0 30.5 38.5 13.0 40.5 52-53 31.88483692969222 36.0 30.5 39.0 10.5 40.0 54-55 31.842610855052204 36.0 30.0 39.0 8.0 40.0 56-57 31.392083137252186 36.0 29.5 39.0 6.5 40.0 58-59 30.915424434940277 35.5 29.0 39.0 2.0 40.0 60-61 30.34857720060716 35.0 28.0 39.0 2.0 40.0 62-63 29.59100111535569 34.5 27.5 38.0 2.0 40.0 64-65 28.910232033199996 34.0 26.0 38.0 2.0 40.0 66-67 28.193557465616593 34.0 25.5 37.5 2.0 39.5 68-69 27.3308981785215 33.0 23.0 36.5 2.0 39.0 70-71 26.479443342929777 32.0 21.0 36.0 2.0 39.0 72-73 25.543682842207737 31.5 18.0 35.5 2.0 38.5 74-75 24.581298270564297 31.0 10.5 35.0 2.0 37.5 76-77 23.61531690750045 30.0 4.5 34.5 2.0 36.5 78-79 22.616771944940414 29.0 2.0 34.0 2.0 36.0 80-81 21.633698217276365 29.0 2.0 34.0 2.0 35.5 82-83 20.621478469136825 27.0 2.0 33.5 2.0 35.0 84-85 19.627946984271986 26.0 2.0 33.0 2.0 35.0 86-87 18.622905680355437 24.5 2.0 32.0 2.0 34.5 88-89 17.546721667427924 21.5 2.0 32.0 2.0 34.0 90-91 16.401907090986263 15.5 2.0 31.0 2.0 34.0 92-93 15.24407315902873 4.5 2.0 31.0 2.0 34.0 94-95 13.994966914190277 2.0 2.0 30.0 2.0 34.0 96-97 12.594650387317932 2.0 2.0 29.0 2.0 34.0 98-99 10.934869052397495 2.0 2.0 27.5 2.0 33.5 100-101 7.741628776868883 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.509667895249752 1101 2 0.42071856630496995 1101 3 0.4607911116867207 1101 4 0.37460011560900597 1101 5 0.41881427302050867 1101 6 0.4354899345247034 1101 7 0.334626506104172 1101 8 0.44069100509731385 1101 9 0.48754789165140267 1101 10-11 0.47724085668703964 1101 12-13 0.5462473682486433 1101 14-15 0.6921778680945962 1101 16-17 0.670772979639807 1101 18-19 0.6182641382438945 1101 20-21 0.6916983720877923 1101 22-23 0.6447740435587761 1101 24-25 0.6769264328096227 1101 26-27 0.5989230957470042 1101 28-29 0.7830057444051306 1101 30-31 0.828443476494563 1101 32-33 0.7899677390583193 1101 34-35 0.9360134869156127 1101 36-37 1.0336692376809253 1101 38-39 0.9808063316365505 1101 40-41 1.0680600245189922 1101 42-43 1.1390528855875957 1101 44-45 1.3016322345108051 1101 46-47 1.3243111123535485 1101 48-49 1.2355234187219892 1101 50-51 1.1556057159623911 1101 52-53 1.2666379666762033 1101 54-55 1.614292458184483 1101 56-57 1.8056758402153292 1101 58-59 1.7029671596722054 1101 60-61 1.8413955943119191 1101 62-63 1.7374563141926593 1101 64-65 1.7268931550656283 1101 66-67 1.862040686226841 1101 68-69 1.988388237943358 1101 70-71 2.2074307980174694 1101 72-73 2.401637763505896 1101 74-75 2.496182870313831 1101 76-77 2.6273620090630523 1101 78-79 2.7167748347927763 1101 80-81 3.1699284726162418 1101 82-83 3.0527243434194986 1101 84-85 3.1484449819342792 1101 86-87 3.232170515227054 1101 88-89 3.5757378568860148 1101 90-91 3.481567845863079 1101 92-93 3.4999648828438623 1101 94-95 3.512323102878364 1101 96-97 3.294282765873623 1101 98-99 2.800518910836008 1101 100-101 2.0950361050539454 1102 1 0.5863338366146387 1102 2 0.48979027597277636 1102 3 0.43109674687897837 1102 4 0.35160959237119727 1102 5 0.32853845638673107 1102 6 0.4040910769857149 1102 7 0.37523592465436195 1102 8 0.5506726538216498 1102 9 0.5438759618166031 1102 10-11 0.6278156197679863 1102 12-13 0.6954418605712789 1102 14-15 0.6649319000109628 1102 16-17 0.7536710983572803 1102 18-19 0.7531285024497905 1102 20-21 0.6420280992421468 1102 22-23 0.5790334718612868 1102 24-25 0.7113923406402023 1102 26-27 0.6902654783774409 1102 28-29 0.7963186216786369 1102 30-31 0.5458886818237403 1102 32-33 0.6222828822121542 1102 34-35 0.7261104807644969 1102 36-37 0.8458888080674356 1102 38-39 1.0674160698294486 1102 40-41 1.070887393100648 1102 42-43 1.355251273002203 1102 44-45 1.1375551333883678 1102 46-47 1.027350908474645 1102 48-49 1.239458264749011 1102 50-51 1.2898260645218933 1102 52-53 1.285280252065217 1102 54-55 1.2594042596630182 1102 56-57 1.5808727636743924 1102 58-59 1.726255435729172 1102 60-61 1.5426098239679575 1102 62-63 1.614344556191984 1102 64-65 1.768573165339287 1102 66-67 2.0349697567448963 1102 68-69 2.0168405150655353 1102 70-71 1.9071503759522166 1102 72-73 2.1362610973833753 1102 74-75 2.300305647332646 1102 76-77 2.5295690030790112 1102 78-79 2.6938197379508964 1102 80-81 3.034146242697723 1102 82-83 3.2399175201346146 1102 84-85 3.3640581796355065 1102 86-87 3.449269809980102 1102 88-89 3.6057375730722754 1102 90-91 3.487220016625553 1102 92-93 3.3364476596774963 1102 94-95 3.2709807303122442 1102 96-97 2.9424569880579146 1102 98-99 2.885012374766603 1102 100-101 2.1366923132178384 1103 1 0.4433819468643847 1103 2 0.3642390507153408 1103 3 0.4386010073756452 1103 4 0.45259473971794506 1103 5 0.3408815144811399 1103 6 0.38093279037358485 1103 7 0.3919251731428872 1103 8 0.30851339032977165 1103 9 0.3877267591227209 1103 10-11 0.52586561566293 1103 12-13 0.4912461634980758 1103 14-15 0.5841003460958092 1103 16-17 0.5972127947022088 1103 18-19 0.7184908010611579 1103 20-21 0.5394387691601636 1103 22-23 0.6872485064975535 1103 24-25 0.732880485734583 1103 26-27 0.8474890380306661 1103 28-29 0.7552715181026599 1103 30-31 0.6520681910693256 1103 32-33 0.8722208752085479 1103 34-35 0.8610237337089046 1103 36-37 0.8122920291935571 1103 38-39 0.9435711029539746 1103 40-41 1.0667897576248038 1103 42-43 1.0393462586306654 1103 44-45 1.066232804937009 1103 46-47 1.127812792393506 1103 48-49 0.9232530588814427 1103 50-51 1.0546241353256995 1103 52-53 1.2498975086262938 1103 54-55 1.2451680839196975 1103 56-57 1.3002046840751191 1103 58-59 1.3765486768843544 1103 60-61 1.5319268962180672 1103 62-63 1.7722039450965532 1103 64-65 1.659745676822677 1103 66-67 1.8212292921479012 1103 68-69 1.8812237017742994 1103 70-71 1.870292205919963 1103 72-73 2.1309732819008893 1103 74-75 2.0441433391028987 1103 76-77 2.3086670528928863 1103 78-79 2.4331004777852208 1103 80-81 2.53779802171195 1103 82-83 2.7006750143599874 1103 84-85 2.800027961028846 1103 86-87 2.7627812438416477 1103 88-89 3.146723220498682 1103 90-91 3.201596789477396 1103 92-93 3.236532654059264 1103 94-95 3.0580244423518135 1103 96-97 2.906694873843742 1103 98-99 2.8398644263913013 1103 100-101 2.09672344652959 1104 1 0.5641845528118168 1104 2 0.5568651026037728 1104 3 0.48389081469853323 1104 4 0.40294221682503206 1104 5 0.4071270323979448 1104 6 0.4889350727541739 1104 7 0.5299925757392288 1104 8 0.5014758481447146 1104 9 0.5722086283310759 1104 10-11 0.6650474375099478 1104 12-13 0.5774134236430797 1104 14-15 0.7021848417500394 1104 16-17 0.7240728655412312 1104 18-19 0.6728301941400403 1104 20-21 0.6673532425223314 1104 22-23 0.7670887086313201 1104 24-25 0.8176675448240118 1104 26-27 0.7513318627163486 1104 28-29 0.9117747552864657 1104 30-31 0.843999803041477 1104 32-33 0.9737755150893648 1104 34-35 0.8795165543398937 1104 36-37 0.9693837701112216 1104 38-39 1.1957421977408202 1104 40-41 1.11882462775678 1104 42-43 1.204329166031961 1104 44-45 1.2182178891959907 1104 46-47 1.2571015432015713 1104 48-49 1.1942534261452522 1104 50-51 1.2633426142602922 1104 52-53 1.3716291569850263 1104 54-55 1.4331453420341411 1104 56-57 1.6346301044975746 1104 58-59 1.8246182275927936 1104 60-61 2.08384044715023 1104 62-63 2.016137594790443 1104 64-65 2.0827009133441443 1104 66-67 2.2708288331737627 1104 68-69 2.5502938539357594 1104 70-71 2.6427989149819204 1104 72-73 2.9123835895878685 1104 74-75 2.6868740919404246 1104 76-77 2.9865249948141432 1104 78-79 3.188297756488115 1104 80-81 3.4035525871848726 1104 82-83 3.3920767158227356 1104 84-85 3.6356605580008647 1104 86-87 3.7071001853137204 1104 88-89 3.7308667643473115 1104 90-91 3.635025748544887 1104 92-93 3.5925528461293226 1104 94-95 3.546846315367807 1104 96-97 3.51864131596305 1104 98-99 2.9182718037741875 1104 100-101 2.072999527517182 1105 1 0.41606465467388176 1105 2 0.4286147198711987 1105 3 0.3195027890677089 1105 4 0.2952528235614196 1105 5 0.3443681982630338 1105 6 0.4583619732240507 1105 7 0.3368544668307294 1105 8 0.34937637585586856 1105 9 0.3806914283985421 1105 10-11 0.4160522640385622 1105 12-13 0.4806654029800441 1105 14-15 0.6407127716397625 1105 16-17 0.6465721370500788 1105 18-19 0.6023684009908479 1105 20-21 0.5920199314251064 1105 22-23 0.5618193366919613 1105 24-25 0.6186004099579563 1105 26-27 0.47833121433551895 1105 28-29 0.6149508732909084 1105 30-31 0.7006104217836437 1105 32-33 0.7054375979285226 1105 34-35 0.662126175559834 1105 36-37 0.7745849340767563 1105 38-39 0.7295776706964148 1105 40-41 0.9133880109688164 1105 42-43 0.88960263010501 1105 44-45 0.99774134523215 1105 46-47 0.9340430162955755 1105 48-49 0.9310154210828259 1105 50-51 0.9292738517627122 1105 52-53 0.9342314764870743 1105 54-55 1.21922129696517 1105 56-57 1.2706289096083339 1105 58-59 1.209475988456191 1105 60-61 1.4617452436773277 1105 62-63 1.5384415763727368 1105 64-65 1.7002899002299223 1105 66-67 1.8131146742274673 1105 68-69 1.9538555874525336 1105 70-71 2.0553808815486327 1105 72-73 2.170033694748291 1105 74-75 2.271601771561862 1105 76-77 2.1819756591351585 1105 78-79 2.48975062769993 1105 80-81 2.5492435781733036 1105 82-83 2.9217354199798287 1105 84-85 3.0438436101379054 1105 86-87 3.104875445297367 1105 88-89 2.9056308655098846 1105 90-91 2.7312799638174887 1105 92-93 2.8633328138766245 1105 94-95 3.0073885972011904 1105 96-97 2.8460452354997052 1105 98-99 2.5377871444921514 1105 100-101 1.920434949933024 1106 1 0.1965515615840232 1106 2 0.09476253009246349 1106 3 -0.025581160903342948 1106 4 0.07810893836602162 1106 5 0.07872780710454919 1106 6 -0.23989073699733865 1106 7 0.08824584112408473 1106 8 -0.180190719211744 1106 9 0.04819085339873652 1106 10-11 0.1386371709512062 1106 12-13 0.21971400476002145 1106 14-15 0.30024881463096165 1106 16-17 -0.17677051733267746 1106 18-19 0.2680250298932876 1106 20-21 0.2877453975088855 1106 22-23 0.19052654094900845 1106 24-25 0.2768653590135983 1106 26-27 0.3382609286973164 1106 28-29 0.35803202903424847 1106 30-31 0.25386709125956486 1106 32-33 0.3059853817686715 1106 34-35 0.23943866614173004 1106 36-37 0.34650987479230366 1106 38-39 0.42362476349324396 1106 40-41 0.6304838925509983 1106 42-43 0.14693159218757756 1106 44-45 0.5786233010078803 1106 46-47 0.8700693105877804 1106 48-49 0.6445912185793787 1106 50-51 0.6148164574739745 1106 52-53 0.670659164470262 1106 54-55 0.42733173699967963 1106 56-57 0.9556745318249114 1106 58-59 1.2039635457109341 1106 60-61 1.3840504773651787 1106 62-63 1.3601465144418157 1106 64-65 1.3363906209937433 1106 66-67 1.4357904958093037 1106 68-69 1.5167709178447062 1106 70-71 1.2469273200353435 1106 72-73 1.6055076552926622 1106 74-75 1.635102579066121 1106 76-77 1.6993279217541613 1106 78-79 2.0610374960402105 1106 80-81 1.9957675947984121 1106 82-83 2.312763669897489 1106 84-85 2.370031835227266 1106 86-87 2.078601272869225 1106 88-89 2.0689773100909647 1106 90-91 1.941310214442268 1106 92-93 2.1333245864747425 1106 94-95 2.106881553818864 1106 96-97 2.122318301757632 1106 98-99 2.0316326221134933 1106 100-101 1.280388206936439 1107 1 0.16191035550709287 1107 2 0.15436215498908723 1107 3 0.1824934328249519 1107 4 0.059322631324569386 1107 5 0.1056548364968748 1107 6 0.010470783527615879 1107 7 0.17912655158946222 1107 8 0.25909273222083584 1107 9 0.2559767764287173 1107 10-11 0.14334093037142992 1107 12-13 0.1351171185032598 1107 14-15 0.1921000268352202 1107 16-17 0.2815956562718114 1107 18-19 0.3018068848894231 1107 20-21 0.33009367786375066 1107 22-23 0.28673393603904174 1107 24-25 0.1474124008210964 1107 26-27 0.3022175390115436 1107 28-29 0.45929665868789016 1107 30-31 0.4522028007558774 1107 32-33 0.37990715016360355 1107 34-35 0.35249617998641014 1107 36-37 0.3597262899388056 1107 38-39 0.6347225347419752 1107 40-41 0.5431801065669717 1107 42-43 0.6431988561519333 1107 44-45 0.42385961252345083 1107 46-47 0.5898223073419402 1107 48-49 0.3516885693014302 1107 50-51 0.7128548690548264 1107 52-53 0.6867622865999614 1107 54-55 0.8319211757610852 1107 56-57 1.0936391425789083 1107 58-59 1.0541733475939168 1107 60-61 1.1653859144019378 1107 62-63 1.3370117689467271 1107 64-65 1.358955769524325 1107 66-67 1.4311981278067343 1107 68-69 1.5459721218029543 1107 70-71 1.6057779018508533 1107 72-73 1.3852060477812849 1107 74-75 1.555327584914572 1107 76-77 1.8730784699837137 1107 78-79 2.137510713747435 1107 80-81 2.4165841468568487 1107 82-83 2.369474180215441 1107 84-85 2.504337623126929 1107 86-87 2.475452178105062 1107 88-89 2.689896225083988 1107 90-91 2.6503289868655067 1107 92-93 2.7815226818259546 1107 94-95 2.6310628008168937 1107 96-97 2.5264563163661187 1107 98-99 2.346004642964875 1107 100-101 1.5220086310504337 1108 1 0.46115724938591995 1108 2 0.315240094227768 1108 3 0.15563640997415717 1108 4 0.3648096093635118 1108 5 0.24789806947643456 1108 6 0.3681251645194976 1108 7 0.31410733699507887 1108 8 0.3870288648939706 1108 9 0.34206284445944135 1108 10-11 0.35646504543955615 1108 12-13 0.3833550010643876 1108 14-15 0.36385704293512333 1108 16-17 0.5605851600573288 1108 18-19 0.4736791601150472 1108 20-21 0.5077954453250157 1108 22-23 0.5775543025959848 1108 24-25 0.629768548825183 1108 26-27 0.5982946946677927 1108 28-29 0.7070388521648425 1108 30-31 0.7508242641905198 1108 32-33 0.8672371036602868 1108 34-35 0.8606862389915975 1108 36-37 0.9380208410711219 1108 38-39 1.0652121884391974 1108 40-41 0.9294505218396125 1108 42-43 1.0822323544954244 1108 44-45 1.0267662905024721 1108 46-47 1.074020879954972 1108 48-49 1.309498340550988 1108 50-51 1.1017100824870347 1108 52-53 1.1298056798645426 1108 54-55 1.3896490082503625 1108 56-57 1.5340109939652962 1108 58-59 1.6004935952244068 1108 60-61 1.7229745003955017 1108 62-63 1.7043156963340778 1108 64-65 1.8633614747612341 1108 66-67 1.954402484182559 1108 68-69 2.3491012648633323 1108 70-71 2.444327371741206 1108 72-73 2.3629767162497863 1108 74-75 2.556155429842846 1108 76-77 2.636683088371978 1108 78-79 2.7259227333881917 1108 80-81 2.816613582840059 1108 82-83 3.2539038548598853 1108 84-85 3.2092308894343375 1108 86-87 2.9003431946142086 1108 88-89 2.8453550394807223 1108 90-91 2.6904936861686686 1108 92-93 2.9160585221001583 1108 94-95 2.9073915402008925 1108 96-97 2.616381635937012 1108 98-99 2.6087671587213226 1108 100-101 1.922507935079909 1109 1 0.6539344741865136 1109 2 0.584390981342402 1109 3 0.4897702861445694 1109 4 0.44055350816002203 1109 5 0.47954370593478757 1109 6 0.5619447184757576 1109 7 0.47416176255948983 1109 8 0.5765865015351608 1109 9 0.6407577657646115 1109 10-11 0.752185908336152 1109 12-13 0.5529083268478132 1109 14-15 0.7763866259010044 1109 16-17 0.7799741688673976 1109 18-19 0.768478823442571 1109 20-21 0.749157101072683 1109 22-23 0.8510173478268968 1109 24-25 0.6415657982299763 1109 26-27 0.6678802533730845 1109 28-29 0.7731605933100525 1109 30-31 0.7251073969152984 1109 32-33 0.8451415539313487 1109 34-35 0.7972730483973081 1109 36-37 0.6892030169746022 1109 38-39 0.8465142960309606 1109 40-41 0.46494917459804697 1109 42-43 0.6323603404737028 1109 44-45 0.8970482064147447 1109 46-47 0.9882246440351992 1109 48-49 1.0934543051783123 1109 50-51 1.1864896416131678 1109 52-53 1.2641961978688734 1109 54-55 1.4130668170579064 1109 56-57 1.6247341777898825 1109 58-59 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0.2159215132053518 2316 90-91 0.3600188732677516 2316 92-93 0.24442504912449436 2316 94-95 0.3309241208003968 2316 96-97 0.5204823039219555 2316 98-99 0.36263885801753126 2316 100-101 0.5886460644449754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11864.0 3 4734.0 4 5277.0 5 6133.0 6 7476.0 7 9544.0 8 12133.0 9 15373.0 10 19261.0 11 23875.0 12 29086.0 13 34361.0 14 39157.0 15 44380.0 16 49939.0 17 57208.0 18 58677.0 19 60039.0 20 66666.0 21 72627.0 22 80533.0 23 89249.0 24 98797.0 25 108606.0 26 119241.0 27 129877.0 28 143262.0 29 157258.0 30 173614.0 31 192788.0 32 214497.0 33 241621.0 34 268677.0 35 290870.0 36 285395.0 37 198643.0 38 46656.0 39 558.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64609298177411 18.11803481222524 14.243220109124351 23.992652096876302 2 15.699063120054527 19.2586934907597 40.30268251506447 24.739560874121306 3 19.606414435682137 22.994522524455952 28.820192324611316 28.5788707152506 4 13.208637402028986 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23.405970091142635 26.209249524314913 27.107380455057044 23.27739992948541 50-51 23.361300237545173 26.418169480420044 26.988600888638697 23.23192939339609 52-53 23.420475831579136 26.456940603169105 26.88099048501219 23.24159308023957 54-55 23.209852654005754 26.30570606154679 27.05500216356618 23.429439120881277 56-57 23.21230310129696 26.607243069217564 27.056092230867858 23.12436159861762 58-59 23.322474846764997 26.22874567682716 27.005550646095088 23.443228830312755 60-61 23.3652225557624 26.292062866788662 27.27180970916735 23.070904868281588 62-63 23.212620024665696 26.339926666192753 27.101843842450407 23.34560946669114 64-65 23.05707505586561 26.177993636698936 27.10986571202732 23.655065595408136 66-67 23.058441089380075 26.348413436604577 27.003175332784192 23.589970141231152 68-69 23.03993810357414 26.120470503962462 27.136048857342782 23.703542535120615 70-71 23.08158505940101 26.145702159615965 27.015192394847976 23.75752038613505 72-73 23.331177655935143 26.094640726441654 26.817131507135727 23.75705011048748 74-75 23.18169345385851 26.311182849314353 26.65729212283094 23.849831573996198 76-77 23.20870291033735 26.18862409575981 26.705961010271906 23.89671198363093 78-79 23.3740876914898 26.100116058576344 26.74549633173287 23.78029991820098 80-81 23.181320913138663 26.38884743344671 26.620709523951657 23.809122129462967 82-83 23.40229487276542 26.377410319357175 26.40669644305536 23.813598364822045 84-85 23.48045805244801 25.945390313383744 26.447209756720735 24.12694187744751 86-87 23.399729256542617 25.900898205621715 26.592794948236786 24.106577589598885 88-89 23.52753800868288 25.87927231323426 26.49327730862201 24.099912369460853 90-91 23.445112630152 26.03892301923798 26.4183467451184 24.09761760549162 92-93 23.581936794439827 25.5755833914068 26.507873406620536 24.33460640753284 94-95 23.438871991865586 25.6710768450278 26.39607619249234 24.493974970614275 96-97 23.490664259428033 25.682328138158578 26.313319797422796 24.513687804990596 98-99 23.663462589410827 25.481181152977555 25.939543673492693 24.915812584118925 100-101 24.71175567979237 24.541351082549152 25.943923102128725 24.802970135529755 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 317.0 1 244.0 2 213.5 3 262.5 4 231.0 5 228.0 6 401.5 7 875.0 8 1428.5 9 1720.5 10 1695.5 11 1580.0 12 1645.0 13 1817.5 14 2035.0 15 2298.0 16 2591.0 17 2830.5 18 3112.0 19 3327.0 20 3694.5 21 4363.5 22 5253.5 23 6560.5 24 8025.5 25 9868.0 26 12582.0 27 16247.5 28 20100.0 29 24021.5 30 28744.0 31 34262.0 32 40345.0 33 46461.0 34 52248.0 35 57794.0 36 64549.0 37 71819.0 38 77886.0 39 83085.0 40 89472.0 41 98410.5 42 106971.5 43 114528.0 44 121673.5 45 129393.0 46 135320.5 47 139207.0 48 143073.5 49 143449.0 50 141715.0 51 138593.5 52 135347.5 53 133911.5 54 131102.5 55 123920.0 56 113837.5 57 104193.5 58 94056.5 59 81794.0 60 69279.5 61 58091.5 62 47840.5 63 38847.5 64 31358.5 65 24894.0 66 19238.5 67 15142.0 68 11848.0 69 8705.0 70 6380.0 71 4772.0 72 3892.5 73 3161.5 74 2389.5 75 1887.5 76 1378.0 77 872.0 78 546.5 79 346.5 80 185.0 81 111.0 82 74.0 83 44.5 84 30.0 85 16.5 86 12.5 87 9.0 88 5.5 89 3.0 90 2.5 91 3.5 92 4.0 93 2.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3852706150488819 2 0.02938333633222144 3 0.03486207421556008 4 0.04305134557802415 5 0.06153487706865608 6 0.053605124869087 7 0.03639035373038612 8 0.039043216284423775 9 0.03546761892898172 10-11 0.03662103743073722 12-13 0.029008475319150896 14-15 0.027090917059982376 16-17 0.022188888427521487 18-19 0.025966334020770757 20-21 0.03383841529525207 22-23 0.03290126276257572 24-25 0.04186909161372476 26-27 0.03607316364240336 28-29 0.03099812223467914 30-31 0.03412676992069095 32-33 0.028345259680641485 34-35 0.029556349107484767 36-37 0.031791097454636055 38-39 0.014893516403917931 40-41 0.012543426206591095 42-43 0.01374009790216243 44-45 0.010856551647773672 46-47 0.01946393721712411 48-49 0.016854327856902288 50-51 0.011563020480098917 52-53 0.009155259357684305 54-55 0.008578550106806554 56-57 0.008823651538429598 58-59 0.0045415853506622925 60-61 0.007799992618121589 62-63 0.011058399885580885 64-65 0.007353042948691332 66-67 0.0036476860118017777 68-69 0.006444725878558873 70-71 0.013278730501460227 72-73 0.01051052609724702 74-75 0.011692780061546412 76-77 0.005550826539698358 78-79 0.008838069269701543 80-81 0.01077004526014201 82-83 0.01267318578803859 84-85 0.007799992618121589 86-87 0.002696115747853488 88-89 0.015960428518041775 90-91 0.024567814087392212 92-93 0.01042401970961536 94-95 0.02040108974980046 96-97 0.018224012327736946 98-99 0.01127466585466004 100-101 0.1192923085440629 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3467952.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.825630580194975 #Duplication Level Percentage of deduplicated Percentage of total 1 76.38320996311005 41.113744420038294 2 12.808754464065213 13.788785719503952 3 4.466872985299345 7.2129676546612576 4 2.0833652457575935 4.485537923270614 5 1.1531769582504257 3.1035238474190168 6 0.6934272214950036 2.239449447506466 7 0.45454555287577314 1.7126340707673295 8 0.3276709182045936 1.4109675036122993 9 0.23710120830533407 1.148590984352469 >10 1.2391557737142334 11.964730179945654 >50 0.08975512999596734 3.37878222070416 >100 0.058550681100493406 6.041530862741913 >500 0.003498821450897231 1.2562231453153032 >1k 8.074203310264128E-4 0.7703123908309316 >5k 1.0765604413685506E-4 0.372219629330311 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7063 0.20366487194747795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5784 0.16678431535384575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3713 0.10706607242545456 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.8835462543887575E-5 2 0.0 0.0 0.0 0.0 2.8835462543887575E-5 3 0.0 0.0 0.0 0.0 2.8835462543887575E-5 4 0.0 0.0 0.0 0.0 2.8835462543887575E-5 5 0.0 0.0 0.0 0.0 2.8835462543887575E-5 6 0.0 0.0 0.0 0.0 2.8835462543887575E-5 7 5.767092508777515E-5 0.0 0.0 0.0 2.8835462543887575E-5 8 5.767092508777515E-5 0.0 0.0 0.0 2.8835462543887575E-5 9 5.767092508777515E-5 0.0 0.0 0.0 8.650638763166272E-5 10-11 5.767092508777515E-5 0.0 0.0 0.0 8.650638763166272E-5 12-13 1.153418501755503E-4 0.0 0.0 0.0 8.650638763166272E-5 14-15 1.153418501755503E-4 0.0 0.0 0.0 1.153418501755503E-4 16-17 1.153418501755503E-4 0.0 0.0 0.0 1.4417731271943788E-4 18-19 1.4417731271943785E-4 0.0 0.0 0.0 1.4417731271943788E-4 20-21 1.7301277526332544E-4 0.0 0.0 1.4417731271943787E-5 1.4417731271943788E-4 22-23 2.306837003511006E-4 0.0 0.0 7.208865635971893E-5 1.4417731271943788E-4 24-25 3.171900879827633E-4 0.0 0.0 3.027723567108195E-4 1.4417731271943788E-4 26-27 3.171900879827633E-4 0.0 0.0 6.920511010533017E-4 1.4417731271943788E-4 28-29 4.0369647561442604E-4 0.0 0.0 0.002090571034431849 1.4417731271943788E-4 30-31 4.613674007022012E-4 2.8835462543887575E-5 0.0 0.007468384798866882 1.4417731271943788E-4 32-33 4.75785131974145E-4 2.8835462543887575E-5 0.0 0.020545267062519898 1.4417731271943788E-4 34-35 5.046205945180326E-4 2.8835462543887575E-5 0.0 0.041595154719557825 1.7301277526332544E-4 36-37 5.190383257899764E-4 2.8835462543887575E-5 0.0 0.0774087991990662 1.7301277526332544E-4 38-39 6.343801759655266E-4 2.8835462543887575E-5 0.0 0.13179248155683815 1.7301277526332544E-4 40-41 6.343801759655266E-4 2.8835462543887575E-5 0.0 0.191337711709966 1.7301277526332544E-4 42-43 6.343801759655266E-4 2.8835462543887575E-5 0.0 0.2554101094824842 1.7301277526332544E-4 44-45 6.343801759655266E-4 2.8835462543887575E-5 0.0 0.3315789837921632 1.7301277526332544E-4 46-47 7.064688323252455E-4 2.8835462543887575E-5 0.0 0.4256546803415964 1.7301277526332544E-4 48-49 7.497220261410769E-4 2.8835462543887575E-5 0.0 0.5310194604769616 1.7301277526332544E-4 50-51 7.497220261410769E-4 2.8835462543887575E-5 0.0 0.642482940940359 1.7301277526332544E-4 52-53 7.497220261410769E-4 2.8835462543887575E-5 0.0 0.754551966117178 2.0184823780721302E-4 54-55 7.497220261410769E-4 2.8835462543887575E-5 0.0 0.878198429505368 2.451014316230444E-4 56-57 7.497220261410769E-4 2.8835462543887575E-5 0.0 1.0211069818728749 2.595191628949882E-4 58-59 7.497220261410769E-4 2.8835462543887575E-5 0.0 1.179067645688291 2.595191628949882E-4 60-61 7.497220261410769E-4 2.8835462543887575E-5 0.0 1.3526138770086784 2.595191628949882E-4 62-63 7.497220261410769E-4 2.8835462543887575E-5 0.0 1.5246174110829678 2.595191628949882E-4 64-65 7.497220261410769E-4 2.8835462543887575E-5 0.0 1.7122786013185882 2.595191628949882E-4 66-67 7.497220261410769E-4 2.8835462543887575E-5 0.0 1.9229072374704148 2.595191628949882E-4 68-69 7.497220261410769E-4 2.8835462543887575E-5 0.0 2.1555517492744998 2.595191628949882E-4 70-71 7.929752199569083E-4 2.8835462543887575E-5 0.0 2.409693098405053 2.8835462543887576E-4 72-73 8.506461450446834E-4 2.8835462543887575E-5 0.0 2.6670207661467056 3.171900879827633E-4 74-75 8.650638763166272E-4 2.8835462543887575E-5 0.0 2.9291639561331877 3.4602555052665087E-4 76-77 8.650638763166272E-4 2.8835462543887575E-5 0.0 3.2183403922545644 3.4602555052665087E-4 78-79 8.650638763166272E-4 2.8835462543887575E-5 0.0 3.5298354763849096 3.7486101307053845E-4 80-81 8.938993388605148E-4 2.8835462543887575E-5 0.0 3.86161630841488 3.7486101307053845E-4 82-83 8.938993388605148E-4 2.8835462543887575E-5 0.0 4.204585299911878 3.7486101307053845E-4 84-85 9.5157026394829E-4 2.8835462543887575E-5 0.0 4.544569821035585 3.7486101307053845E-4 86-87 0.0010236589203080088 2.8835462543887575E-5 0.0 4.903109962306283 3.7486101307053845E-4 88-89 0.0010380766515799527 2.8835462543887575E-5 0.0 5.284545460836828 3.7486101307053845E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8710 0.0 43.34081 1 GGTATCA 5300 0.0 42.771687 1 ATCAACG 11090 0.0 33.9409 3 TCAACGC 11270 0.0 33.48315 4 TATCAAC 11280 0.0 33.45347 2 CAACGCA 11635 0.0 32.269367 5 AACGCAG 12135 0.0 31.01183 6 ACGCAGA 13725 0.0 27.204416 7 CGCAGAG 14000 0.0 26.432524 8 GTACATG 11145 0.0 23.937037 1 GCAGAGT 15195 0.0 23.914698 9 TACATGG 11340 0.0 22.924717 2 ACATGGG 11445 0.0 22.25762 3 GTGGTAT 2880 0.0 21.873592 1 GAGTACT 8340 0.0 21.272844 12-13 TGACGCG 425 0.0 20.692644 94-95 GGACCGA 800 0.0 20.194399 6 GTACTTT 8795 0.0 20.146912 14-15 AGTACTT 8745 0.0 19.907429 12-13 AGAGTAC 14595 0.0 19.673458 10-11 >>END_MODULE