FastQCFastQC Report
Thu 26 May 2016
SRR938644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3467952
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73870.2130075618116975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63150.18209594596465004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42530.12263722219915385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55300.046.912871
GTATCAA88300.040.088521
ATCAACG113600.030.8159683
TATCAAC114650.030.6201382
TCAACGC116400.030.1554364
CAACGCA119250.029.5144025
AACGCAG124700.028.2625686
ACGCAGA143950.024.318127
GTACATG110450.024.0045151
CGCAGAG146600.023.7813368
TACATGG111400.023.2832832
GTGGTAT28150.022.2802491
ACATGGG113800.022.163533
GCAGAGT158900.021.4921159
GAGTACT88200.021.11016712-13
TGGTATC30800.019.7422222
GTACTTT93400.019.73145114-15
CATGGGG74650.019.4699924
CAGAGTA156150.018.7983910-11
AGAGTAC152850.018.66043310-11
Produced by FastQC (version 0.11.5)