##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938644_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3467952 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.800120070866033 31.0 31.0 34.0 30.0 34.0 2 32.06360209137843 33.0 31.0 34.0 30.0 34.0 3 32.12574107138738 33.0 31.0 34.0 30.0 34.0 4 35.7738322214379 37.0 35.0 37.0 35.0 37.0 5 35.65983179697989 37.0 35.0 37.0 33.0 37.0 6 35.68752595191629 37.0 35.0 37.0 33.0 37.0 7 35.61003900861373 37.0 35.0 37.0 33.0 37.0 8 35.5847260861742 37.0 35.0 37.0 33.0 37.0 9 37.127988507338046 39.0 37.0 39.0 33.0 39.0 10-11 37.05237284137728 39.0 37.0 39.0 33.0 39.0 12-13 36.925805778165326 39.0 37.0 39.0 33.0 39.0 14-15 37.95134203126226 40.0 37.0 41.0 33.0 41.0 16-17 38.0402592077399 40.0 37.0 41.0 33.0 41.0 18-19 38.163531963533515 40.0 37.5 41.0 33.0 41.0 20-21 38.115139136873864 40.0 37.0 41.0 33.0 41.0 22-23 37.97986664751992 40.0 37.0 41.0 33.0 41.0 24-25 37.85749701841317 40.0 37.0 41.0 32.0 41.0 26-27 37.64432639206079 40.0 37.0 41.0 32.0 41.0 28-29 37.49413010906726 39.5 36.5 41.0 32.0 41.0 30-31 37.25372006302278 39.0 36.0 41.0 31.0 41.0 32-33 37.180287818285834 39.0 36.0 41.0 31.0 41.0 34-35 37.05374656281286 39.0 36.0 41.0 31.0 41.0 36-37 36.9313052775817 39.0 36.0 41.0 30.5 41.0 38-39 36.72628831079554 39.0 35.0 40.0 30.0 41.0 40-41 36.50920312622551 39.0 35.0 40.0 30.0 41.0 42-43 36.255825628497746 38.5 35.0 40.0 30.0 41.0 44-45 35.96761258517996 38.0 35.0 40.0 29.0 41.0 46-47 35.56363395456454 38.0 34.5 40.0 27.5 41.0 48-49 35.16771771927639 38.0 34.0 40.0 26.5 41.0 50-51 34.77198357993421 38.0 33.5 40.0 26.0 41.0 52-53 34.16278238568469 37.5 33.0 40.0 24.0 41.0 54-55 33.712151581106085 37.0 32.5 40.0 23.0 41.0 56-57 33.29996854051036 37.0 32.0 39.0 22.0 40.5 58-59 32.737587775147986 36.0 31.0 39.0 20.5 40.0 60-61 32.22280210337398 36.0 31.0 39.0 18.5 40.0 62-63 31.67604439161788 35.0 30.0 38.5 15.5 40.0 64-65 30.98795715742317 34.5 29.0 38.0 10.0 40.0 66-67 30.29859453072015 34.0 28.0 38.0 7.5 40.0 68-69 29.54550207153963 34.0 27.5 37.0 4.5 39.0 70-71 28.741198695945045 33.0 26.0 36.0 2.0 39.0 72-73 28.12362267413159 33.0 26.0 36.0 2.0 38.5 74-75 27.384545114811274 32.0 25.0 35.0 2.0 38.0 76-77 26.234139918891614 30.5 23.0 34.0 2.0 36.0 78-79 26.712178398086248 32.0 25.0 35.0 2.0 36.0 80-81 26.31028529229932 32.0 23.5 35.0 2.0 36.0 82-83 25.601649907495837 31.0 21.0 34.0 2.0 36.0 84-85 24.795711272820384 31.0 19.0 34.0 2.0 35.0 86-87 23.851144566014753 30.0 11.5 34.0 2.0 35.0 88-89 22.86245224847403 29.5 4.5 34.0 2.0 35.0 90-91 21.979566902886777 29.0 2.0 34.0 2.0 35.0 92-93 20.85895479522208 28.0 2.0 33.0 2.0 35.0 94-95 19.650856326731166 26.5 2.0 33.0 2.0 34.0 96-97 18.258286014339298 24.5 2.0 32.0 2.0 34.0 98-99 16.479450119263472 11.0 2.0 32.0 2.0 34.0 100-101 12.343767877986778 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28930150223486706 1101 2 0.2831848640166399 1101 3 0.25334648314134967 1101 4 0.18678506888216617 1101 5 0.185919579835236 1101 6 0.2488647054501314 1101 7 0.2271564928420986 1101 8 0.2473484259624854 1101 9 0.31310278916931367 1101 10-11 0.2807138242292311 1101 12-13 0.23351009188125715 1101 14-15 0.4828803346367678 1101 16-17 0.31445845294374664 1101 18-19 0.37114620566280365 1101 20-21 0.47407495556068113 1101 22-23 0.3754898708172618 1101 24-25 0.34416915115313174 1101 26-27 0.4828881779237335 1101 28-29 0.5175822330300406 1101 30-31 0.6184712339317215 1101 32-33 0.5265283415002742 1101 34-35 0.2828897379765536 1101 36-37 0.4894551707891779 1101 38-39 0.6866875408713113 1101 40-41 0.655069624159502 1101 42-43 0.6148571044793769 1101 44-45 0.751468882412972 1101 46-47 0.7170683426416531 1101 48-49 0.7115273887665836 1101 50-51 0.8401134243990853 1101 52-53 0.8939936496861804 1101 54-55 1.0063722023255366 1101 56-57 1.0614239455789516 1101 58-59 1.1213339390523132 1101 60-61 1.0281242247941194 1101 62-63 1.3251536447590695 1101 64-65 1.4010484182314862 1101 66-67 1.5035536066882536 1101 68-69 1.573545705031929 1101 70-71 1.8368546937281884 1101 72-73 1.8038620675630845 1101 74-75 1.8538861673779046 1101 76-77 1.925059841939639 1101 78-79 2.011839189337582 1101 80-81 2.1306199682723026 1101 82-83 2.371114494814659 1101 84-85 2.551788947307408 1101 86-87 2.632120471976272 1101 88-89 2.728019924092738 1101 90-91 2.944587131613847 1101 92-93 3.0964970761238853 1101 94-95 2.76478489103566 1101 96-97 2.5767485537821813 1101 98-99 2.613273800886713 1101 100-101 2.2399711668259368 1102 1 0.3031117580557172 1102 2 0.2433176888470001 1102 3 0.22500043488535937 1102 4 0.14629903885870021 1102 5 0.1994626280390861 1102 6 0.25208438110939824 1102 7 0.27339689676742296 1102 8 0.2790090563872738 1102 9 0.3168081687681763 1102 10-11 0.34192615776793644 1102 12-13 0.3242969242119571 1102 14-15 0.5720746397631089 1102 16-17 0.4535290978068076 1102 18-19 0.4685670835248672 1102 20-21 0.38707245739590945 1102 22-23 0.48638928461105024 1102 24-25 0.4789357908407226 1102 26-27 0.5308707151259853 1102 28-29 0.43766949267151034 1102 30-31 0.4956579835739845 1102 32-33 0.47692036677701566 1102 34-35 0.5847433134358866 1102 36-37 0.6240463391094409 1102 38-39 0.7563744822388188 1102 40-41 0.7777966233203344 1102 42-43 0.6570954327399576 1102 44-45 0.690015860732629 1102 46-47 0.6652177361558387 1102 48-49 0.7888150029940704 1102 50-51 0.882559077590372 1102 52-53 0.888847814713202 1102 54-55 0.8116636478372286 1102 56-57 1.0453899789115724 1102 58-59 1.154184071106208 1102 60-61 1.0897550829562945 1102 62-63 1.3987619962903608 1102 64-65 1.447310259152065 1102 66-67 1.5569585462953022 1102 68-69 1.7433580578538717 1102 70-71 1.7597842171405063 1102 72-73 1.8559658419436396 1102 74-75 1.7542989955979245 1102 76-77 1.7727458139121275 1102 78-79 1.862568661099825 1102 80-81 2.0793259688395302 1102 82-83 2.1261174668074787 1102 84-85 2.2265818286952026 1102 86-87 2.2589911517423857 1102 88-89 1.970200603945404 1102 90-91 2.1515821540039326 1102 92-93 2.227790067112096 1102 94-95 1.9767375456703675 1102 96-97 2.1292456416720498 1102 98-99 2.0754043926464156 1102 100-101 2.14040679072402 1103 1 0.20607149196735364 1103 2 0.1971863941781251 1103 3 0.2165948813953733 1103 4 0.15160132024701056 1103 5 0.10669603121431948 1103 6 0.16916715013668693 1103 7 0.21903651349947495 1103 8 0.17781458450923537 1103 9 0.3137651553073155 1103 10-11 0.29429874355435715 1103 12-13 0.3485949996620974 1103 14-15 0.4122306435403331 1103 16-17 0.3590699421002981 1103 18-19 0.25049384063186864 1103 20-21 0.2774523617810658 1103 22-23 0.4238702262591332 1103 24-25 0.38935351351053527 1103 26-27 0.4400451363005473 1103 28-29 0.3572653056827235 1103 30-31 0.4283348553783668 1103 32-33 0.4713355212216399 1103 34-35 0.54404971444594 1103 36-37 0.511321294880652 1103 38-39 0.46649396774689933 1103 40-41 0.6186405719301646 1103 42-43 0.46471459659718306 1103 44-45 0.644222598836599 1103 46-47 0.6777200150150122 1103 48-49 0.818870269460362 1103 50-51 0.9154663788204402 1103 52-53 1.0183417178128948 1103 54-55 1.0420985820609374 1103 56-57 1.0507785288309677 1103 58-59 1.0813590128617747 1103 60-61 1.0218125553295252 1103 62-63 1.124155418633407 1103 64-65 1.2815337087302971 1103 66-67 1.127039010261928 1103 68-69 1.1414101134638805 1103 70-71 1.4830602523940186 1103 72-73 1.6667933233709995 1103 74-75 1.5163728297728518 1103 76-77 1.5187523856486003 1103 78-79 1.5352803200546958 1103 80-81 1.5899487337493028 1103 82-83 1.969468663458823 1103 84-85 2.1068573457627195 1103 86-87 2.1189750744687785 1103 88-89 1.8823407753156154 1103 90-91 1.991866339118861 1103 92-93 2.201801205093176 1103 94-95 2.03848986311327 1103 96-97 1.978296357317653 1103 98-99 1.8937675572003094 1103 100-101 1.561353920520654 1104 1 0.21126911506616253 1104 2 0.26751304956759014 1104 3 0.2772883698605426 1104 4 0.1889345717824895 1104 5 0.15658053919333526 1104 6 0.1380357801784271 1104 7 0.11345648236424921 1104 8 0.18246245989411847 1104 9 0.2318410823912629 1104 10-11 0.2754164800264576 1104 12-13 0.22581905106029154 1104 14-15 0.36166955831300385 1104 16-17 0.40301876358208233 1104 18-19 0.3267805198296472 1104 20-21 0.37296795047169695 1104 22-23 0.4629121866304544 1104 24-25 0.3560411531228951 1104 26-27 0.5246796509608416 1104 28-29 0.46189820214142685 1104 30-31 0.4379611343844303 1104 32-33 0.5997485880249798 1104 34-35 0.4762107455250444 1104 36-37 0.5618487670670049 1104 38-39 0.7314060581648718 1104 40-41 0.6758337121881013 1104 42-43 0.8360551259989037 1104 44-45 0.8495742633387735 1104 46-47 0.8405422283860062 1104 48-49 0.9483319070160832 1104 50-51 0.8930520367419916 1104 52-53 0.8652877664024743 1104 54-55 1.0230551871658449 1104 56-57 1.1741238327539278 1104 58-59 1.1614244499217747 1104 60-61 1.0503104445499432 1104 62-63 1.0825456423447264 1104 64-65 1.3468308521452919 1104 66-67 1.2959822410500799 1104 68-69 1.3605640640385666 1104 70-71 1.5480523987896433 1104 72-73 1.7458358127344447 1104 74-75 1.69938568969987 1104 76-77 1.7843312724710145 1104 78-79 1.8229186374887227 1104 80-81 2.052764720442216 1104 82-83 2.16707858338356 1104 84-85 2.4954413192668277 1104 86-87 2.2936105727237894 1104 88-89 2.3851890213439724 1104 90-91 2.490911673651734 1104 92-93 2.6653292647730353 1104 94-95 2.4618743546990345 1104 96-97 2.6463710675360446 1104 98-99 2.5743977943715386 1104 100-101 2.2414985594564367 1105 1 0.1017322285921054 1105 2 0.11845096091946061 1105 3 0.11635435327534083 1105 4 0.08494843884106018 1105 5 0.010812756058491857 1105 6 -0.04617642677356315 1105 7 0.09300132988271059 1105 8 0.14984363826831526 1105 9 0.07608178776202834 1105 10-11 0.16038723135444144 1105 12-13 0.1559681045302952 1105 14-15 0.25172966389660445 1105 16-17 0.19507972258412565 1105 18-19 0.2637690731457667 1105 20-21 0.2591832775463345 1105 22-23 0.20693720173679253 1105 24-25 0.2565038365783394 1105 26-27 0.3023100211429366 1105 28-29 0.3119335687601392 1105 30-31 0.2458433440228518 1105 32-33 0.3201531662848396 1105 34-35 0.4221986747649211 1105 36-37 0.3284499837269621 1105 38-39 0.30507993493321095 1105 40-41 0.37238390042366376 1105 42-43 0.24553666352074544 1105 44-45 0.31528230373506716 1105 46-47 0.4554418526043662 1105 48-49 0.49737101835081887 1105 50-51 0.4845672457670247 1105 52-53 0.4222446565325697 1105 54-55 0.46804736100326494 1105 56-57 0.5292707897380069 1105 58-59 0.675037042937447 1105 60-61 0.6484550308027437 1105 62-63 0.7061953481170349 1105 64-65 0.8407563434232621 1105 66-67 0.8915859639264418 1105 68-69 1.0622014949611867 1105 70-71 1.1455685563829903 1105 72-73 1.2663769362216435 1105 74-75 1.14204725205893 1105 76-77 1.138511044868057 1105 78-79 1.1431812752481 1105 80-81 1.3011949415906692 1105 82-83 1.5528847832763617 1105 84-85 1.651738417480363 1105 86-87 1.775328224290675 1105 88-89 1.6638936197111356 1105 90-91 1.7418653916025413 1105 92-93 1.8635309150888375 1105 94-95 1.5176015793420916 1105 96-97 1.6967013814987126 1105 98-99 1.9651606644465538 1105 100-101 1.8334568100399853 1106 1 -0.031104622153808492 1106 2 -0.02138431572536348 1106 3 -0.00665377853673732 1106 4 0.035858775187769254 1106 5 0.031658012028366045 1106 6 -0.043640834181481125 1106 7 0.026503886388184128 1106 8 -0.07629119845469745 1106 9 -0.13458252797985892 1106 10-11 -0.06721947963416852 1106 12-13 -0.06254534462114236 1106 14-15 -0.0455752880309106 1106 16-17 -0.16672296753835525 1106 18-19 -0.09770176591147361 1106 20-21 -0.0903079467670338 1106 22-23 0.013485061547875432 1106 24-25 -0.06351483891430831 1106 26-27 -0.26318548258819874 1106 28-29 0.02068709024258908 1106 30-31 0.018004045092659737 1106 32-33 -0.15231559275496664 1106 34-35 -0.10655599045579578 1106 36-37 -0.08157851372238412 1106 38-39 -0.041146876712709 1106 40-41 0.02722857116688715 1106 42-43 -0.030022275941682608 1106 44-45 -0.013173361588755483 1106 46-47 -0.4721663611060478 1106 48-49 -0.15890223992155939 1106 50-51 0.2070131091704468 1106 52-53 0.03786131720922015 1106 54-55 -0.03854199062653407 1106 56-57 0.11125707907122262 1106 58-59 0.259574452988069 1106 60-61 0.27435535943964595 1106 62-63 0.37407880202292887 1106 64-65 0.5033992220037611 1106 66-67 0.48871856201138897 1106 68-69 0.5276676956171222 1106 70-71 0.27933350481249875 1106 72-73 0.5862660854431745 1106 74-75 0.5492561007527961 1106 76-77 0.6923847068716924 1106 78-79 0.8572452903621439 1106 80-81 0.9732613468672255 1106 82-83 1.3757379260311744 1106 84-85 1.4821260821379738 1106 86-87 1.4963613204861588 1106 88-89 1.4805239497297151 1106 90-91 1.5113038286382867 1106 92-93 1.344516266473196 1106 94-95 1.2634096325117312 1106 96-97 1.285433555983083 1106 98-99 1.220186120971352 1106 100-101 1.2589727340907562 1107 1 -0.06125494037774004 1107 2 0.05225793083408092 1107 3 0.03423192768926242 1107 4 0.03601237977953531 1107 5 0.004335815964225276 1107 6 -0.025362529786775667 1107 7 -0.025676813283141087 1107 8 0.008608081794633904 1107 9 0.03605724638029528 1107 10-11 0.0613126936661601 1107 12-13 0.06622726721467131 1107 14-15 0.0817353515749204 1107 16-17 -0.03063014189201141 1107 18-19 -0.01194815040528141 1107 20-21 0.04707394562708345 1107 22-23 0.14979356762284368 1107 24-25 0.21139572894517045 1107 26-27 0.21189048708091462 1107 28-29 0.09713346326019234 1107 30-31 0.03984470801605511 1107 32-33 0.07367544376705126 1107 34-35 0.16611952626436022 1107 36-37 0.22509036525702442 1107 38-39 0.07224878391744483 1107 40-41 0.21227551727245952 1107 42-43 0.12470168721728925 1107 44-45 0.17651197771969152 1107 46-47 0.3369514036181016 1107 48-49 0.3161831594965321 1107 50-51 0.3476820515498673 1107 52-53 0.3257738637109213 1107 54-55 0.3606210088040669 1107 56-57 0.3433778938175891 1107 58-59 0.5423097061647937 1107 60-61 0.5346890601988079 1107 62-63 0.6836957816703162 1107 64-65 0.8124813543147731 1107 66-67 0.8502615578546759 1107 68-69 1.0184813144217273 1107 70-71 0.9374987244474013 1107 72-73 1.3146339804784297 1107 74-75 1.306039962479769 1107 76-77 1.3383722283043085 1107 78-79 1.4649165315939818 1107 80-81 1.496940489827434 1107 82-83 1.6544704313389182 1107 84-85 1.8418305979444405 1107 86-87 1.983509535675438 1107 88-89 1.9525409088322938 1107 90-91 1.9519656284844409 1107 92-93 2.0481864050263034 1107 94-95 1.9744613384335707 1107 96-97 2.0942552265895102 1107 98-99 2.087492900399088 1107 100-101 1.703753929807002 1108 1 0.0694601347225614 1108 2 0.0996363171135144 1108 3 0.0950070168691397 1108 4 0.05730829950170602 1108 5 0.059022828311469766 1108 6 0.05490370083116147 1108 7 0.11021811170786577 1108 8 0.07228111470679721 1108 9 0.19350420585204375 1108 10-11 0.12177149384260844 1108 12-13 0.11811116265577937 1108 14-15 0.20533074324563216 1108 16-17 0.1807704376069239 1108 18-19 0.12082991335816473 1108 20-21 0.08991306828659873 1108 22-23 0.14563043045504998 1108 24-25 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-0.22375439432222777 2316 58-59 -0.21417889417693914 2316 60-61 0.0937686615849671 2316 62-63 0.03845218578918619 2316 64-65 0.0404897791569887 2316 66-67 7.610736468173229E-4 2316 68-69 0.2631249600484402 2316 70-71 0.2996272469884893 2316 72-73 0.5108401935151825 2316 74-75 0.6271885886986297 2316 76-77 0.5185071586750674 2316 78-79 0.4073337713081564 2316 80-81 -0.10546510281843169 2316 82-83 0.4152460156656481 2316 84-85 0.6628913440088056 2316 86-87 0.8296618345495332 2316 88-89 0.6946037078381018 2316 90-91 0.7519581652605858 2316 92-93 0.7711433509641239 2316 94-95 0.9948998555492565 2316 96-97 0.9351389472035834 2316 98-99 0.6030972460484527 2316 100-101 0.36370486257819756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 19.0 4 51.0 5 114.0 6 246.0 7 587.0 8 1511.0 9 2753.0 10 4531.0 11 7060.0 12 10315.0 13 14748.0 14 20057.0 15 26590.0 16 32954.0 17 41196.0 18 42632.0 19 42155.0 20 45122.0 21 49413.0 22 54160.0 23 60635.0 24 68026.0 25 77075.0 26 88365.0 27 102284.0 28 120102.0 29 139146.0 30 161388.0 31 186852.0 32 217671.0 33 257194.0 34 309253.0 35 371051.0 36 425596.0 37 370328.0 38 114754.0 39 2016.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18577576932595 18.986215095730586 14.931502345170033 23.89650678977343 2 16.178177601398573 19.94469260386863 39.25781154245574 24.619318252277054 3 19.56160098457535 23.244386862216835 29.400549209880012 27.7934629433278 4 13.714809201511441 16.78157598490406 35.70513663395572 33.79847817962878 5 14.565620524189097 36.48386206594276 33.841001607002646 15.1095158028655 6 33.491524680849096 35.310869354593144 16.842764836422187 14.354841128135568 7 29.976741315912097 31.421369153898326 19.834645923588333 18.767243606601244 8 26.581592268620174 35.50428279571908 18.900184403155183 19.01394053250556 9 26.222627842465556 16.852598372520863 19.486611648413756 37.43816213659983 10-11 25.644530258780975 26.114894323796868 26.95619777897733 21.284377638444823 12-13 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27.72242596278228 22.968824522379133 38-39 22.888684999151423 26.259859056532687 27.802269059184404 23.049186885131494 40-41 23.218336508463842 26.04157834843611 27.694967720948423 23.045117422151627 42-43 23.15024416183024 25.90859229328718 27.666055709384267 23.27510783549831 44-45 23.138758936171687 26.26496282506442 27.501796461748917 23.094481777014977 46-47 23.109744501831845 26.482774191846165 27.121436710241394 23.286044596080597 48-49 23.29803007654085 26.270620239265135 27.244537986684936 23.18681169750908 50-51 23.248908866097338 26.45114465252114 27.114951418012705 23.184995063368813 52-53 23.379720941927683 26.47216570471564 26.931788559933935 23.216324793422746 54-55 23.14292412351728 26.315473801252153 27.10977545248608 23.431826622744488 56-57 23.124152237401212 26.610936368208094 27.07226051571648 23.192650878674215 58-59 23.2772973789718 26.29748047262477 27.014964451641777 23.410257696761665 60-61 23.26486929461538 26.36120684484676 27.271657739207463 23.102266121330402 62-63 23.11234411548949 26.43726037730626 27.044636142599437 23.405759364604815 64-65 22.934505631141423 26.279553868614485 27.025326511622005 23.760613988622087 66-67 22.93088053523564 26.42759132572005 27.007375147800683 23.634152991243624 68-69 22.909923004201513 26.207378407179068 27.102108715708695 23.780589872910724 70-71 22.90483301164967 26.305385182001533 26.962415708524183 23.82736609782461 72-73 23.133579387080204 26.253571463071108 26.749715933789542 23.86313321605915 74-75 23.012256224999653 26.448318777191844 26.585892768988728 23.95353222881978 76-77 22.89806779332586 26.33222720499015 26.915467688134093 23.854237313549902 78-79 23.004831093394603 26.28474961591164 26.89329321743784 23.817126073255913 80-81 22.771213454417477 26.534780741283058 26.87818280933688 23.81582299496258 82-83 22.94950646052131 26.554863463833684 26.691141549561998 23.80448852608301 84-85 22.925923645666455 26.215721271890715 26.68230401326041 24.176051069182424 86-87 22.78695260999706 26.120545472371692 26.876250785796262 24.216251131834984 88-89 22.824436439720042 26.24975778211463 26.69144497963063 24.234360798534695 90-91 22.746527224089025 26.288912925188505 26.824568045876717 24.139991804845753 92-93 22.803116897197643 25.930149806686163 26.81390314008102 24.452830156035173 94-95 22.588099597745064 26.007973009991492 26.742167562969478 24.661759829293963 96-97 22.593881111319366 26.05359073804409 26.723006387058636 24.629521763577905 98-99 22.62289097426954 25.922172509884795 26.48904886803508 24.965887647810582 100-101 22.78333477270371 25.672778295774894 26.494055640628194 25.0498312908932 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 391.0 1 268.5 2 179.5 3 246.0 4 223.5 5 230.5 6 455.0 7 1022.5 8 1677.5 9 1929.5 10 1757.5 11 1645.0 12 1802.0 13 1980.5 14 2214.5 15 2451.5 16 2695.0 17 2916.0 18 3155.5 19 3409.0 20 3818.0 21 4510.0 22 5441.5 23 6811.0 24 8309.0 25 10276.0 26 13245.0 27 16837.5 28 20437.5 29 24539.5 30 29432.0 31 35019.0 32 41264.5 33 47194.0 34 52621.5 35 58173.0 36 65861.5 37 73516.0 38 79087.0 39 84111.5 40 90456.0 41 100106.0 42 108875.0 43 115857.5 44 123300.5 45 131742.0 46 137432.5 47 141382.5 48 145004.5 49 144430.5 50 142885.0 51 139685.0 52 135231.5 53 133317.0 54 132145.5 55 125104.5 56 113083.5 57 102935.5 58 92663.5 59 79239.0 60 65687.0 61 54189.0 62 44725.0 63 35969.5 64 28588.0 65 22561.5 66 16985.5 67 13623.0 68 10518.5 69 7323.0 70 5384.0 71 3974.5 72 3197.5 73 2633.5 74 2098.5 75 1783.5 76 1251.5 77 685.0 78 391.0 79 229.5 80 129.0 81 81.5 82 49.5 83 23.0 84 14.0 85 11.0 86 5.0 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023818092061251135 2 0.012197400656064443 3 0.001758963215177142 4 0.0 5 1.4417731271943788E-4 6 0.0 7 0.0 8 2.01848237807213E-4 9 3.4602555052665087E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014706085897382662 26-27 0.008102764974832408 28-29 2.162659690791568E-4 30-31 0.0 32-33 2.8835462543887576E-4 34-35 0.009241765745315968 36-37 9.804057264921776E-4 38-39 0.011289083585931985 40-41 0.010899804841589504 42-43 0.004902028632460888 44-45 6.920511010533017E-4 46-47 4.325319381583136E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.4417731271943788E-4 66-67 6.199624446935828E-4 68-69 0.0010236589203080088 70-71 1.4417731271943786E-5 72-73 3.027723567108195E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5859504399138167E-4 82-83 0.0012399248893871656 84-85 0.005348978301891145 86-87 0.0038062810557931595 88-89 0.0 90-91 0.0014129376646504913 92-93 4.6136740070220115E-4 94-95 5.767092508777514E-5 96-97 5.767092508777514E-5 98-99 0.0 100-101 1.4417731271943786E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3467952.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.595435318476504 #Duplication Level Percentage of deduplicated Percentage of total 1 73.59892135322238 37.237694648366016 2 14.296680619093552 14.466935590645289 3 4.987920404394483 7.570980125827507 4 2.3312377774098185 4.717999607157095 5 1.2629738511149784 3.195035589650753 6 0.7884674541080504 2.3935712445028625 7 0.5188752471039816 1.8376903302245606 8 0.3738671401549292 1.5132776565930008 9 0.26404552871549825 1.202354862233213 >10 1.3975939762512524 12.771389183800895 >50 0.10565833415148024 3.7195323863868355 >100 0.06845435562882224 6.690352931715066 >500 0.0042773860120645804 1.4337140889250917 >1k 9.12509012231218E-4 0.8540937725644311 >5k 1.1406362652890225E-4 0.39537798140740676 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7387 0.2130075618116975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6315 0.18209594596465004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4253 0.12263722219915385 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.8835462543887575E-5 0.0 0.0 0.0 0.0 6 2.8835462543887575E-5 0.0 0.0 0.0 0.0 7 2.8835462543887575E-5 0.0 0.0 0.0 0.0 8 1.4417731271943788E-4 0.0 0.0 0.0 0.0 9 1.4417731271943788E-4 0.0 0.0 0.0 0.0 10-11 1.4417731271943788E-4 0.0 0.0 0.0 0.0 12-13 1.7301277526332544E-4 0.0 0.0 0.0 0.0 14-15 2.0184823780721302E-4 0.0 0.0 0.0 0.0 16-17 2.162659690791568E-4 0.0 0.0 0.0 1.4417731271943787E-5 18-19 2.595191628949882E-4 0.0 0.0 0.0 5.767092508777515E-5 20-21 2.8835462543887576E-4 0.0 0.0 0.0 7.208865635971893E-5 22-23 2.8835462543887576E-4 0.0 0.0 4.325319381583136E-5 1.153418501755503E-4 24-25 3.4602555052665087E-4 0.0 0.0 2.7393689416693197E-4 1.7301277526332544E-4 26-27 3.4602555052665087E-4 0.0 0.0 6.776333697813581E-4 1.7301277526332544E-4 28-29 3.6044328179859466E-4 0.0 0.0 0.002104988765703793 1.7301277526332544E-4 30-31 3.7486101307053845E-4 0.0 0.0 0.0075404734552266 1.7301277526332544E-4 32-33 4.0369647561442604E-4 0.0 0.0 0.02074711530032711 1.7301277526332544E-4 34-35 5.334560570619202E-4 0.0 0.0 0.041897927076268646 2.0184823780721302E-4 36-37 7.064688323252455E-4 0.0 0.0 0.07775482474959285 2.0184823780721302E-4 38-39 7.497220261410769E-4 0.0 0.0 0.13254220358297925 2.0184823780721302E-4 40-41 7.785574886849645E-4 0.0 0.0 0.1921306869299229 2.0184823780721302E-4 42-43 7.929752199569083E-4 0.0 0.0 0.2562751733588008 2.0184823780721302E-4 44-45 8.073929512288521E-4 0.0 0.0 0.3328765796066382 2.162659690791568E-4 46-47 8.073929512288521E-4 0.0 0.0 0.4272838839753261 2.306837003511006E-4 48-49 8.073929512288521E-4 0.0 0.0 0.5328216768859546 2.306837003511006E-4 50-51 8.073929512288521E-4 0.0 0.0 0.6448474488689578 2.306837003511006E-4 52-53 8.218106825007959E-4 0.0 0.0 0.7567002080766978 2.595191628949882E-4 54-55 8.506461450446834E-4 0.0 0.0 0.8800583168394488 2.8835462543887576E-4 56-57 9.227348014044024E-4 0.0 0.0 1.022822691894236 2.8835462543887576E-4 58-59 9.227348014044024E-4 0.0 0.0 1.1807256847845644 2.8835462543887576E-4 60-61 9.227348014044024E-4 0.0 0.0 1.3545314352678468 2.8835462543887576E-4 62-63 9.227348014044024E-4 0.0 0.0 1.5260880196727058 2.8835462543887576E-4 64-65 9.227348014044024E-4 0.0 0.0 1.7126102091378428 3.027723567108195E-4 66-67 9.5157026394829E-4 0.0 0.0 1.9236713772278278 3.171900879827633E-4 68-69 9.5157026394829E-4 0.0 0.0 2.1581325231721777 3.171900879827633E-4 70-71 9.5157026394829E-4 0.0 0.0 2.4161666597461555 3.7486101307053845E-4 72-73 9.659879952202338E-4 0.0 0.0 2.6781224192261024 4.613674007022012E-4 74-75 9.804057264921776E-4 0.0 0.0 2.948108855024522 4.613674007022012E-4 76-77 9.804057264921776E-4 0.0 0.0 3.2482716023751195 4.613674007022012E-4 78-79 9.948234577641215E-4 0.0 0.0 3.5723966190996874 4.613674007022012E-4 80-81 0.0010669121141238403 0.0 0.0 3.918739359714321 4.75785131974145E-4 82-83 0.0010669121141238403 0.0 0.0 4.280076540851777 5.478737883338639E-4 84-85 0.001081329845395784 0.0 0.0 4.640375645337652 5.478737883338639E-4 86-87 0.0010957475766677279 0.0 0.0 5.022301346731442 5.478737883338639E-4 88-89 0.0010957475766677279 0.0 0.0 5.434057334126885 5.478737883338639E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5530 0.0 46.91287 1 GTATCAA 8830 0.0 40.08852 1 ATCAACG 11360 0.0 30.815968 3 TATCAAC 11465 0.0 30.620138 2 TCAACGC 11640 0.0 30.155436 4 CAACGCA 11925 0.0 29.514402 5 AACGCAG 12470 0.0 28.262568 6 ACGCAGA 14395 0.0 24.31812 7 GTACATG 11045 0.0 24.004515 1 CGCAGAG 14660 0.0 23.781336 8 TACATGG 11140 0.0 23.283283 2 GTGGTAT 2815 0.0 22.280249 1 ACATGGG 11380 0.0 22.16353 3 GCAGAGT 15890 0.0 21.492115 9 GAGTACT 8820 0.0 21.110167 12-13 TGGTATC 3080 0.0 19.742222 2 GTACTTT 9340 0.0 19.731451 14-15 CATGGGG 7465 0.0 19.469992 4 CAGAGTA 15615 0.0 18.79839 10-11 AGAGTAC 15285 0.0 18.660433 10-11 >>END_MODULE