FastQCFastQC Report
Thu 26 May 2016
SRR938643_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938643_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186135
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56910.47979361539791004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.39363141632276255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31360.26438811771004145No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13620.1148267271432004No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37300.049.7313841
GTATCAA63300.039.598771
ATCAACG79700.031.329783
TATCAAC79700.031.2104282
TCAACGC80800.030.9032634
CAACGCA81950.030.527645
AACGCAG84400.029.6696
ACGCAGA95700.026.1194217
CGCAGAG96900.025.6488368
GTACATG74250.025.175081
TACATGG74950.024.3677832
GCAGAGT100650.023.9839869
CGTTCGC601.715873E-423.74119246-47
TAGCCCT8350.023.3535984
GAGTACT54300.022.83987612-13
ACATGGG75600.022.7742023
GTAGGAC13600.022.0322073
TAGGACC7700.021.6189064
GTACTTT56850.021.56009914-15
TGGTATC17250.021.5061132
Produced by FastQC (version 0.11.5)