##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938643_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1186135 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.140123173163257 31.0 31.0 34.0 28.0 34.0 2 31.164614483174343 31.0 31.0 34.0 28.0 34.0 3 31.195954929244984 31.0 31.0 34.0 27.0 34.0 4 34.865491702040664 37.0 35.0 37.0 32.0 37.0 5 34.858099626096525 37.0 35.0 37.0 32.0 37.0 6 34.89554561664566 37.0 35.0 37.0 32.0 37.0 7 34.813702487490886 37.0 35.0 37.0 32.0 37.0 8 34.78496714117701 37.0 35.0 37.0 32.0 37.0 9 36.27091941473778 39.0 35.0 39.0 32.0 39.0 10-11 36.097374666458705 39.0 35.0 39.0 31.0 39.0 12-13 35.967204829129905 38.0 35.0 39.0 30.0 39.0 14-15 36.89207552260072 39.0 36.0 41.0 31.0 41.0 16-17 36.74996859548028 39.0 36.0 41.0 30.0 41.0 18-19 36.9534896955237 39.0 36.0 41.0 31.0 41.0 20-21 36.972051663596474 39.0 36.0 41.0 31.0 41.0 22-23 36.8833357923002 39.0 36.0 41.0 30.0 41.0 24-25 36.761449582045884 39.0 36.0 41.0 30.0 41.0 26-27 36.565367770110484 39.0 36.0 41.0 30.0 41.0 28-29 36.35543761882079 39.0 35.5 40.5 29.5 41.0 30-31 36.08666972983682 38.5 35.0 40.0 29.0 41.0 32-33 35.800879326552206 38.0 35.0 40.0 27.5 41.0 34-35 35.567639855497056 38.0 35.0 40.0 27.0 41.0 36-37 35.28670808971997 38.0 34.0 40.0 26.5 41.0 38-39 35.11780657345075 38.0 34.0 40.0 25.0 41.0 40-41 34.984191934307646 38.0 34.0 40.0 25.0 41.0 42-43 34.68591307060326 38.0 33.5 40.0 24.0 41.0 44-45 34.28564581603274 38.0 33.0 40.0 23.5 41.0 46-47 33.81168163826209 38.0 33.0 40.0 20.5 41.0 48-49 33.410917812896514 38.0 32.5 40.0 17.5 41.0 50-51 32.725801447558666 37.5 31.5 39.5 15.0 40.5 52-53 32.769901402454195 37.0 32.0 39.5 15.0 40.0 54-55 32.90619954726907 38.0 32.0 40.0 13.5 41.0 56-57 32.62709598823068 37.0 31.5 40.0 9.0 41.0 58-59 32.29045977059947 37.0 31.0 40.0 8.0 41.0 60-61 31.863996088134993 36.5 31.0 39.5 6.5 41.0 62-63 31.337352830832916 36.0 30.0 39.0 2.0 40.5 64-65 30.85000821997496 35.0 29.0 39.0 2.0 40.0 66-67 30.349480033891588 35.0 28.5 38.5 2.0 40.0 68-69 29.802768234644454 34.5 28.0 38.0 2.0 40.0 70-71 29.187519127249427 34.0 27.0 37.5 2.0 40.0 72-73 28.572516619103222 34.0 26.0 37.0 2.0 39.0 74-75 27.914145944601586 33.5 26.0 36.0 2.0 39.0 76-77 27.172689449345985 33.0 24.5 36.0 2.0 38.5 78-79 26.404012190855173 32.0 22.0 35.0 2.0 37.5 80-81 25.713500992720054 32.0 20.0 35.0 2.0 37.0 82-83 24.987341238560536 31.0 17.5 35.0 2.0 36.0 84-85 24.273509339156167 31.0 10.5 34.0 2.0 36.0 86-87 23.504513398559187 30.5 4.5 34.0 2.0 35.0 88-89 22.657735839512366 29.5 2.0 34.0 2.0 35.0 90-91 21.75072694086255 29.0 2.0 34.0 2.0 35.0 92-93 20.818822478048453 28.5 2.0 34.0 2.0 35.0 94-95 19.80710121529168 26.5 2.0 33.5 2.0 35.0 96-97 18.5340243732796 25.0 2.0 33.0 2.0 34.5 98-99 17.019504946738778 20.0 2.0 32.0 2.0 34.0 100-101 13.526792481462902 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21810707791776807 1101 2 0.26412976747476336 1101 3 0.23036009067929086 1101 4 0.13904105360060726 1101 5 0.004741200608329166 1101 6 0.24517996458832414 1101 7 0.12380227194255866 1101 8 0.014761961198196616 1101 9 0.21406944250170312 1101 10-11 0.1659932724816997 1101 12-13 0.34872015517653665 1101 14-15 0.2935421145982602 1101 16-17 0.299990590442583 1101 18-19 0.35914509520423366 1101 20-21 0.5893166136604933 1101 22-23 0.5114803007007751 1101 24-25 0.322251477148221 1101 26-27 0.4977027689833875 1101 28-29 0.38110653478833 1101 30-31 0.4122071405563261 1101 32-33 0.5797506196355116 1101 34-35 0.23457278667092396 1101 36-37 0.24167276746207023 1101 38-39 0.2072571088177071 1101 40-41 0.3707901708011647 1101 42-43 0.4246843799157318 1101 44-45 0.28099272327940383 1101 46-47 0.1683080866906579 1101 48-49 0.07961219050599055 1101 50-51 0.26071127729826316 1101 52-53 0.14363037825581415 1101 54-55 0.2234380312941866 1101 56-57 0.27973017624678675 1101 58-59 0.3424200040589227 1101 60-61 0.25008276745418456 1101 62-63 0.1410609644399763 1101 64-65 0.23570972602687945 1101 66-67 0.1121151599713599 1101 68-69 0.29423937499393205 1101 70-71 0.5392131020342497 1101 72-73 0.34552294760053215 1101 74-75 0.13109697928492636 1101 76-77 0.1479969696955088 1101 78-79 0.19837776260408546 1101 80-81 0.22959096581136862 1101 82-83 0.2309978282608789 1101 84-85 0.4722566582766632 1101 86-87 0.6400062939580735 1101 88-89 0.36672753471583874 1101 90-91 0.10456196620854996 1101 92-93 0.03093532698276036 1101 94-95 0.2840195443987561 1101 96-97 0.36052273550299674 1101 98-99 0.49405149576314855 1101 100-101 0.8860370793672399 1102 1 0.42753969322598095 1102 2 0.41143872520025937 1102 3 0.45038202458787424 1102 4 0.3707903862717856 1102 5 0.3789212655823633 1102 6 0.3328121370798911 1102 7 0.420422173860743 1102 8 0.2470234299423737 1102 9 0.5117239627491799 1102 10-11 0.7018143455878274 1102 12-13 0.6728531450165818 1102 14-15 0.5793321491545171 1102 16-17 0.7187083879470819 1102 18-19 0.5586738265552427 1102 20-21 0.6144449063333255 1102 22-23 0.542888726632448 1102 24-25 0.6930354074563283 1102 26-27 0.5285343951964876 1102 28-29 0.40571234520486854 1102 30-31 0.5283171825618282 1102 32-33 0.6684587951895509 1102 34-35 0.6104315198842443 1102 36-37 0.8119854291211865 1102 38-39 0.26651746017409295 1102 40-41 0.4870191939145343 1102 42-43 0.4584155239966776 1102 44-45 0.6949823150190113 1102 46-47 0.6647671054586297 1102 48-49 0.7430275690728294 1102 50-51 0.5584692593786755 1102 52-53 0.3070224779102446 1102 54-55 0.2306648403243301 1102 56-57 0.41922000594861686 1102 58-59 0.3561545623803539 1102 60-61 0.3118520682591246 1102 62-63 0.5270149860014222 1102 64-65 0.5821025292217819 1102 66-67 0.24740443925623268 1102 68-69 0.33446274909122664 1102 70-71 0.8279285199165933 1102 72-73 0.39600197588699615 1102 74-75 0.4627697501997332 1102 76-77 0.4726886534333765 1102 78-79 0.5594750788041409 1102 80-81 0.7885538643980787 1102 82-83 0.8902074833191307 1102 84-85 0.8544860538526358 1102 86-87 1.0148355819337382 1102 88-89 0.8629227258098453 1102 90-91 0.8658315463604325 1102 92-93 1.1568975220290092 1102 94-95 1.1348224082222913 1102 96-97 0.7972377188456399 1102 98-99 1.0830002200787483 1102 100-101 0.8279122216272601 1103 1 0.23821330202332547 1103 2 0.31438647853912727 1103 3 0.22563883203657298 1103 4 0.0555442207712602 1103 5 0.19433319351548306 1103 6 0.3273005361960486 1103 7 0.17663536424055337 1103 8 0.37455524389724815 1103 9 0.4697885537386668 1103 10-11 0.6397292696769199 1103 12-13 0.6454679479333407 1103 14-15 0.6097648885610525 1103 16-17 0.7382986066374642 1103 18-19 0.7303618517113435 1103 20-21 0.49922965552647725 1103 22-23 0.6261022966082308 1103 24-25 0.7139090417810863 1103 26-27 0.6636581285642933 1103 28-29 0.8028758184582685 1103 30-31 0.6755138083017513 1103 32-33 0.8370760712817997 1103 34-35 0.7316900545774914 1103 36-37 0.7884504789410798 1103 38-39 0.674245802069052 1103 40-41 0.8206683046819734 1103 42-43 0.7409248522775727 1103 44-45 0.8634752692868091 1103 46-47 0.6630647252758664 1103 48-49 0.6482276194646133 1103 50-51 0.832428561386223 1103 52-53 0.785173746075742 1103 54-55 0.7189114550410522 1103 56-57 0.691391145714519 1103 58-59 0.5518097632595556 1103 60-61 0.4325511547423524 1103 62-63 0.5489407973670914 1103 64-65 0.3922929405281046 1103 66-67 0.38332921957956856 1103 68-69 0.5408315903952463 1103 70-71 0.3485406640039308 1103 72-73 0.6778946679186291 1103 74-75 0.9305496773637216 1103 76-77 0.8811678991480072 1103 78-79 1.0239364420675408 1103 80-81 0.8880792865535554 1103 82-83 0.9563089829718585 1103 84-85 1.2097978281239854 1103 86-87 1.0770889067137297 1103 88-89 1.1515221658643462 1103 90-91 1.3061454672552806 1103 92-93 1.0982511877823242 1103 94-95 1.1296187110569953 1103 96-97 1.3682093187680628 1103 98-99 1.1941828853065957 1103 100-101 1.2538081261348388 1104 1 0.30763459160258577 1104 2 0.18490187925810986 1104 3 0.36101408088384446 1104 4 0.22756021234448554 1104 5 0.32597427465039175 1104 6 0.4171765073713871 1104 7 0.26691664820300787 1104 8 0.32072566042032236 1104 9 0.47487324544033527 1104 10-11 0.4488728460916036 1104 12-13 0.6359932753148243 1104 14-15 0.5874415672055804 1104 16-17 0.6964541456139983 1104 18-19 0.6662251873966838 1104 20-21 0.6909083739601201 1104 22-23 0.7136914790355533 1104 24-25 0.57804951805862 1104 26-27 0.6649456384734549 1104 28-29 0.5166942616681922 1104 30-31 0.9173929659668758 1104 32-33 0.744472601773225 1104 34-35 0.6504831871318899 1104 36-37 0.8456773770846624 1104 38-39 0.6870539973224581 1104 40-41 0.8854833427977056 1104 42-43 0.9828448524020459 1104 44-45 0.9266639995686532 1104 46-47 0.8638929325564035 1104 48-49 0.5872828905924123 1104 50-51 0.9174384452613893 1104 52-53 0.9168945672503384 1104 54-55 1.2384913300553464 1104 56-57 1.1849808244749624 1104 58-59 0.9982108424340268 1104 60-61 1.277344363536006 1104 62-63 1.3940860292627981 1104 64-65 1.5339306998096554 1104 66-67 1.6519811928149153 1104 68-69 1.643454298647061 1104 70-71 1.824433499614198 1104 72-73 1.7410141605074756 1104 74-75 1.3809529285790774 1104 76-77 1.6609255205454083 1104 78-79 1.7013308020739792 1104 80-81 1.6044109024290591 1104 82-83 1.5575247657428761 1104 84-85 1.7525793318577634 1104 86-87 1.969342220204112 1104 88-89 1.9003993872079157 1104 90-91 1.6746786472802846 1104 92-93 1.9020459579248516 1104 94-95 1.8864395962570093 1104 96-97 1.9999278060878432 1104 98-99 1.9914945957343377 1104 100-101 1.5544323545040886 1105 1 0.38211407083774773 1105 2 0.33172592012448376 1105 3 0.2263161422925144 1105 4 0.3214169043851314 1105 5 0.21140873827926043 1105 6 0.3619629725393523 1105 7 0.4124499756020228 1105 8 0.21613192648245416 1105 9 0.47230043283355627 1105 10-11 0.5658931069908846 1105 12-13 0.5004895381322285 1105 14-15 0.568507274427553 1105 16-17 0.5685834208331642 1105 18-19 0.5022604286122245 1105 20-21 0.6736811538324545 1105 22-23 0.6573781816718238 1105 24-25 0.7704936038917864 1105 26-27 0.6297952323078917 1105 28-29 0.7086803524000373 1105 30-31 0.8087551639987822 1105 32-33 0.7868769899118746 1105 34-35 1.0845096388614976 1105 36-37 0.9594145022537148 1105 38-39 0.8846526816274363 1105 40-41 0.9167278691101757 1105 42-43 0.719209807018899 1105 44-45 0.9229088704542363 1105 46-47 0.6096615028938288 1105 48-49 0.38898824037720203 1105 50-51 0.4679216372718855 1105 52-53 0.4182409149188686 1105 54-55 0.40548725547824205 1105 56-57 0.5359174172785544 1105 58-59 0.6307892974255935 1105 60-61 0.7199553519727111 1105 62-63 0.7688232922611 1105 64-65 0.9915159026914289 1105 66-67 1.0651275904465543 1105 68-69 1.0238827609608059 1105 70-71 1.266839179902032 1105 72-73 1.1589942728957396 1105 74-75 0.793783467520143 1105 76-77 0.9062870638883993 1105 78-79 1.0268490256847045 1105 80-81 1.1787227612000208 1105 82-83 1.2176916827036237 1105 84-85 1.3106542592820496 1105 86-87 1.6045128700402955 1105 88-89 1.4791788517987214 1105 90-91 1.2996280381037302 1105 92-93 1.265621205514993 1105 94-95 1.6597661194855462 1105 96-97 1.7509830612585908 1105 98-99 1.7678781285850782 1105 100-101 1.494111600003821 1106 1 0.20235971666478747 1106 2 0.14840321354596142 1106 3 0.07993259474617886 1106 4 0.1145167846943167 1106 5 0.35215730926039157 1106 6 -0.1124601065348898 1106 7 0.24461716242037568 1106 8 0.05228134984118071 1106 9 -0.028619620412079882 1106 10-11 0.15295559230918343 1106 12-13 0.27469337047558895 1106 14-15 0.4756014722194166 1106 16-17 0.16279939004913757 1106 18-19 0.11791727392023432 1106 20-21 0.3074104736082859 1106 22-23 0.31849236894880306 1106 24-25 0.228235474424352 1106 26-27 0.23540280303174654 1106 28-29 0.426263157192146 1106 30-31 0.20370829441703364 1106 32-33 0.20832569624430164 1106 34-35 -0.0351657976744022 1106 36-37 0.27301143503669323 1106 38-39 0.3899548729761406 1106 40-41 0.6000254070588724 1106 42-43 0.119729399398949 1106 44-45 0.35895398382493227 1106 46-47 0.41173959276261485 1106 48-49 0.7256228589885652 1106 50-51 0.2957290581025589 1106 52-53 0.30338806750788194 1106 54-55 0.3025531525441352 1106 56-57 -0.3872958896556753 1106 58-59 -0.03726164388115194 1106 60-61 0.6371841180386859 1106 62-63 0.4332667299603514 1106 64-65 0.5850788857892972 1106 66-67 0.13104982497344153 1106 68-69 0.08005634620416302 1106 70-71 -0.042044227818582414 1106 72-73 0.399087620547224 1106 74-75 0.7257750332326438 1106 76-77 0.7932841107691679 1106 78-79 0.9037744258659188 1106 80-81 1.2205208640446372 1106 82-83 0.8686335487617676 1106 84-85 0.7476557805858981 1106 86-87 0.33214774593098184 1106 88-89 0.6767330202831197 1106 90-91 0.5408064333518929 1106 92-93 0.6643244617763919 1106 94-95 0.807711520337925 1106 96-97 0.7607610739435806 1106 98-99 1.4515336203801041 1106 100-101 0.8774871607049626 1107 1 0.32647216762394393 1107 2 0.2902399018480999 1107 3 0.2863688128980435 1107 4 0.27454705830680126 1107 5 0.2533809569097727 1107 6 0.3904977216810792 1107 7 0.3898723352768201 1107 8 0.20804002208118533 1107 9 0.28411120919722777 1107 10-11 0.3025311733780143 1107 12-13 0.3510416427134544 1107 14-15 0.4090784180077236 1107 16-17 0.43581081218684403 1107 18-19 0.47512985512010886 1107 20-21 0.6034928221131892 1107 22-23 0.44561780653889826 1107 24-25 0.34019903376678684 1107 26-27 0.37888187492622194 1107 28-29 0.5564831490086419 1107 30-31 0.4818718468061647 1107 32-33 0.49753731200476636 1107 34-35 0.3998438834631557 1107 36-37 0.49759343453926164 1107 38-39 0.47041294856924765 1107 40-41 0.45904016410779036 1107 42-43 0.6390395247456127 1107 44-45 0.44784494533305264 1107 46-47 0.19756448184405428 1107 48-49 0.3805387775065512 1107 50-51 0.45059951271487364 1107 52-53 0.3362554626667773 1107 54-55 0.43476219023371243 1107 56-57 0.5493585151281906 1107 58-59 0.3108651763465389 1107 60-61 0.33891248189969403 1107 62-63 0.6182209183895218 1107 64-65 0.6399270764595677 1107 66-67 0.5423734798435618 1107 68-69 0.6690683219317854 1107 70-71 0.7321609561364006 1107 72-73 0.6965141629102263 1107 74-75 0.5583124139233178 1107 76-77 0.6987867142463564 1107 78-79 0.8479220177498092 1107 80-81 0.9590251817785038 1107 82-83 0.7333022104915905 1107 84-85 0.5456532961171696 1107 86-87 0.8018340182383845 1107 88-89 0.9921261627215365 1107 90-91 0.8926037953756243 1107 92-93 1.0806147140475808 1107 94-95 0.952942624067834 1107 96-97 1.3823673271026529 1107 98-99 1.5897084585152257 1107 100-101 1.5213028660774608 1108 1 0.25585997984246944 1108 2 0.33291165611892026 1108 3 0.3287002548041613 1108 4 0.29742945147795297 1108 5 0.4691606762637619 1108 6 0.5899444184502798 1108 7 0.48926588287554296 1108 8 0.5874239253063323 1108 9 0.5130606228810635 1108 10-11 0.6817610162758996 1108 12-13 0.5361196681922138 1108 14-15 0.7584297550477075 1108 16-17 0.8562621399634978 1108 18-19 0.7103093501832731 1108 20-21 0.6142129900882196 1108 22-23 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68-69 -2.220986472380904 2316 70-71 -2.2272507505526598 2316 72-73 -1.7138359452602465 2316 74-75 -1.9393327554056405 2316 76-77 -2.3366244531952383 2316 78-79 -2.628855324985725 2316 80-81 -2.7027759047701316 2316 82-83 -2.7554013137007196 2316 84-85 -2.7478172574110182 2316 86-87 -2.871058700111611 2316 88-89 -3.1157886945383737 2316 90-91 -2.9191967951120255 2316 92-93 -3.126876798932585 2316 94-95 -3.324100724075347 2316 96-97 -3.5427006186621224 2316 98-99 -3.1765417698187246 2316 100-101 -2.741247330917112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4051.0 3 1385.0 4 1429.0 5 1550.0 6 1928.0 7 2368.0 8 2927.0 9 3806.0 10 4777.0 11 6177.0 12 7944.0 13 9490.0 14 11440.0 15 13486.0 16 16177.0 17 19449.0 18 17281.0 19 14245.0 20 14809.0 21 15944.0 22 17376.0 23 19441.0 24 21929.0 25 24415.0 26 27545.0 27 31188.0 28 35147.0 29 40300.0 30 46656.0 31 54653.0 32 63950.0 33 75747.0 34 92831.0 35 113865.0 36 136180.0 37 140452.0 38 70246.0 39 3551.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75117511413889 19.254587687754785 14.93042059600946 22.063816602096868 2 14.776684215056957 20.672977110575115 42.01342882268249 22.536909851685436 3 19.21174657459095 25.88346603962452 29.781266721921103 25.12352066386343 4 12.237549755400929 17.06420345781046 37.80515801876441 32.8930887680242 5 12.899516004282669 38.60009389449367 34.17506256818328 14.325327533040392 6 30.511325134871253 36.8054014323821 18.351857649539795 14.331415783206852 7 27.95540694953345 33.44993190452297 20.83738462770959 17.757276518233986 8 26.086780532313746 35.81354008375484 20.027119970451377 18.07255941348004 9 27.10964121911463 16.750219457835847 20.573465882212066 35.566673440837455 10-11 24.58052399864536 27.34930810973581 27.680376262118255 20.389791629500575 12-13 26.219273284147814 24.92991421846182 28.083658403988487 20.767154093401878 14-15 22.204630186359392 26.787718613254718 26.834521393086852 24.17312980729904 16-17 20.879837709583306 29.615260224216133 28.195219078147595 21.309682988052966 18-19 21.038485136636677 27.409395576939698 31.25390322737077 20.29821605905285 20-21 22.16327589370259 26.59042260412207 30.944896336615496 20.301405165559842 22-23 21.447781547515135 27.154969939085426 30.855974949378417 20.54127356402102 24-25 21.16730631352537 26.973134831934548 31.384673780102062 20.474885074438024 26-27 20.493019854159048 27.6142912154214 31.067184773627666 20.825504156791887 28-29 20.503560256545974 27.703532251921835 30.852998076369115 20.93990941516307 30-31 20.822329222741757 27.22523468757184 31.032287863736734 20.920148225949664 32-33 20.36037821788859 27.513580080299604 31.124869260096492 21.00117244171531 34-35 20.95593119967809 27.682601384054145 30.922901005162263 20.4385664111055 36-37 20.85819891509446 27.339881117565735 30.766164982906535 21.035754984433268 38-39 20.855340554991 27.720581724342946 30.624312164515487 20.799765556150568 40-41 21.50647090003091 27.11047990206889 30.75215594761546 20.63089325028474 42-43 20.732566072710128 27.604305025312513 30.788082413472345 20.875046488505006 44-45 20.74362985699296 27.962190798867343 30.34929499802076 20.94488434611894 46-47 21.21079000932557 27.76866674648773 29.72273703062262 21.297806213564073 48-49 21.229579484187393 27.929485852610153 29.451536489545177 21.389398173657277 50-51 21.198866156178386 28.454269367162034 29.144035600402677 21.2028288762569 52-53 21.261032548759058 28.262674994688187 29.33169819465851 21.14459426189424 54-55 20.963427543737833 28.096513072552593 29.56672994577221 21.373329437937365 56-57 21.10058209171054 28.402466648931707 28.98531722459414 21.51163403476361 58-59 21.244034712188434 28.068420939461948 28.973158892626717 21.714385455722905 60-61 20.95496576548164 27.882485730873974 29.638293413792216 21.524255089852172 62-63 21.102941796443538 27.89816274736301 29.613620458512152 21.3852749976813 64-65 20.929253139758956 28.01662846526483 29.272658518252147 21.781459876724067 66-67 20.866658909669773 28.38171338570993 29.02874810501625 21.722879599604056 68-69 21.291811162909134 27.726626178208235 29.53068969500437 21.450872963878258 70-71 21.32308822345055 27.862546977719287 29.109678137529766 21.704686661300396 72-73 21.243324290433108 28.028661805369914 28.99123735378372 21.736776550413257 74-75 20.981912079330826 28.28247818596261 29.129108236585854 21.606501498120707 76-77 21.1238685674677 27.750517334718516 29.383473622253742 21.742140475560046 78-79 21.02628438361685 27.953025269278875 29.134855063024602 21.885835284079675 80-81 21.18385026399823 28.212842644408614 28.874303118446154 21.729003973147005 82-83 21.124500768502443 28.255048382350832 28.94847113120356 21.671979717943163 84-85 21.156853801475282 27.929632005962503 29.02407849730077 21.889435695261444 86-87 21.037077259502873 28.170782966054286 28.702403421932527 22.089736352510315 88-89 21.289042568892448 27.807501739442113 28.76784245925489 22.13561323241055 90-91 21.27453849455909 27.98410948783839 28.82097156288188 21.920380454720643 92-93 21.27468933695561 27.876292206891407 28.904449741337253 21.94456871481573 94-95 21.172207237069614 27.77770749094565 28.80717060638418 22.242914665600555 96-97 21.62483796067874 27.4255293846765 28.60908061187397 22.34055204277079 98-99 21.52134050059219 27.354344606067098 28.54045033311394 22.583864560226775 100-101 21.946524795051367 27.004539790449144 28.955358867861808 22.093576546637678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 516.0 1 335.0 2 248.5 3 316.0 4 249.5 5 254.0 6 466.0 7 915.5 8 1384.0 9 1592.0 10 1453.0 11 1268.5 12 1284.0 13 1407.0 14 1624.5 15 1836.5 16 1952.0 17 2137.5 18 2437.5 19 2688.5 20 2910.5 21 3361.5 22 3947.0 23 4586.0 24 5489.5 25 6503.0 26 7825.5 27 9759.0 28 11773.0 29 13948.0 30 16822.0 31 20026.0 32 23775.5 33 27609.0 34 31244.0 35 34907.5 36 38202.0 37 40567.0 38 41116.0 39 40624.0 40 40507.5 41 41262.5 42 42288.5 43 42979.5 44 43215.5 45 43559.5 46 43137.0 47 42832.0 48 43258.5 49 42352.0 50 40842.5 51 38232.5 52 35013.5 53 32913.0 54 31257.5 55 28584.0 56 25013.5 57 21999.5 58 19606.0 59 16760.0 60 13716.5 61 11274.0 62 9148.5 63 6937.0 64 5034.5 65 3808.0 66 2790.0 67 2057.0 68 1616.5 69 1217.5 70 883.5 71 701.5 72 583.5 73 458.0 74 341.0 75 262.5 76 192.5 77 133.0 78 96.5 79 64.0 80 35.5 81 21.0 82 10.0 83 7.0 84 7.0 85 4.0 86 5.0 87 3.0 88 1.0 89 0.0 90 2.0 91 3.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006744594839541873 2 0.10293937873850784 3 0.110695662803981 4 0.11288765612683212 5 0.15402968464803754 6 0.09408709801160914 7 0.025292230648282024 8 0.024702078599822114 9 0.02175131835752254 10-11 0.04877185143343717 12-13 0.05256568603067947 14-15 0.02596669013223621 16-17 0.030308523060191293 18-19 0.035198354318859155 20-21 0.05796136190231297 22-23 0.031699595745846805 24-25 0.04931984976414996 26-27 0.05610659832143896 28-29 0.05197553398221957 30-31 0.06597056827426895 32-33 0.048476775409207216 34-35 0.05939458830571562 36-37 0.050078616683598406 38-39 0.0295076024229957 40-41 0.03401805022193932 42-43 0.030182061906949886 44-45 0.00628090394432337 46-47 0.01256180788864674 48-49 0.008725819573657298 50-51 0.006828902275036147 52-53 0.008346436113933068 54-55 0.010074738541565674 56-57 0.012941191348370969 58-59 0.017831022607038827 60-61 0.022805161301200957 62-63 0.010538429436784177 64-65 0.008135667525197385 66-67 0.009189510468875802 68-69 0.009948277388324264 70-71 0.028116529737340183 72-73 0.025798075261247666 74-75 0.026641149616190404 76-77 0.020992551438074082 78-79 0.004257525492460807 80-81 0.004004603185977987 82-83 0.005227061000644952 84-85 0.004721216387679311 86-87 0.011676579815956867 88-89 0.0345660485526521 90-91 0.03338574445573227 92-93 0.027357762817891724 94-95 0.043586944150539354 96-97 0.03928726494033141 98-99 0.02356392822064942 100-101 0.07064963094420112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1186135.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.49963509583323 #Duplication Level Percentage of deduplicated Percentage of total 1 85.36671073031937 60.18321955819066 2 9.44687536260909 13.320025317195158 3 2.418615011322733 5.115344272066716 4 0.93515555002198 2.637125001375712 5 0.5018048982310432 1.7688531107295138 6 0.27859736617728703 1.178460759249538 7 0.18997051500594322 0.9374996390820557 8 0.13064577430896723 0.7368383532475818 9 0.10228080146457985 0.648968326250602 >10 0.5699487489395472 7.29999377821315 >50 0.038063753641945704 1.8052986452300472 >100 0.01977357505011092 2.667493475986752 >500 0.001078555089741257 0.445581157909022 >1k 3.595183632470857E-4 0.7744866277117946 >5k 1.1983945441569523E-4 0.48081197756166927 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5691 0.47979361539791004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4669 0.39363141632276255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3136 0.26438811771004145 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1362 0.1148267271432004 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 8.430743549427342E-5 0.0 0.0 0.0 0.0 9 8.430743549427342E-5 0.0 0.0 0.0 0.0 10-11 1.2646115324141014E-4 0.0 0.0 0.0 0.0 12-13 3.372297419770937E-4 0.0 0.0 0.0 0.0 14-15 4.2153717747136707E-4 0.0 0.0 0.0 0.0 16-17 4.6369089521850377E-4 0.0 0.0 8.430743549427342E-5 0.0 18-19 5.90152048459914E-4 0.0 0.0 8.430743549427342E-5 0.0 20-21 5.90152048459914E-4 0.0 0.0 2.1076858873568353E-4 0.0 22-23 5.90152048459914E-4 0.0 0.0 5.479983307127772E-4 0.0 24-25 8.430743549427341E-4 0.0 0.0 0.0015175338388969215 0.0 26-27 8.852280726898709E-4 0.0 0.0 0.0025292230648282024 0.0 28-29 0.001306765250161238 0.0 0.0 0.005564290742622046 0.0 30-31 0.001433226403402648 0.0 0.0 0.015681183001934856 0.0 32-33 0.0014753801211497847 0.0 0.0 0.034144511375180736 0.0 34-35 0.0017704561453797418 0.0 0.0 0.0636099600804293 0.0 36-37 0.0021076858873568353 0.0 0.0 0.12072824762779953 0.0 38-39 0.002234147040598246 0.0 0.0 0.20537291286405004 0.0 40-41 0.0022763007583453823 0.0 0.0 0.29022834668903624 0.0 42-43 0.0023606081938396554 0.0 0.0 0.37322901693314847 0.0 44-45 0.0026978379358167494 0.0 0.0 0.4764213179781391 0.0 46-47 0.0029507602422995695 0.0 0.0 0.6040206215987219 0.0 48-49 0.0029507602422995695 0.0 0.0 0.7474697230922281 0.0 50-51 0.0029507602422995695 0.0 0.0 0.9038178622163582 0.0 52-53 0.0029507602422995695 0.0 0.0 1.0476041934518414 0.0 54-55 0.0029507602422995695 0.0 0.0 1.2063129407698112 0.0 56-57 0.003035067677793843 0.0 0.0 1.3981545102370303 0.0 58-59 0.003035067677793843 0.0 0.0 1.6140658525378644 0.0 60-61 0.003035067677793843 0.0 0.0 1.8481876009054619 0.0 62-63 0.0031193751132881165 0.0 0.0 2.082309349273059 0.0 64-65 0.00320368254878239 0.0 0.0 2.324018766835141 0.0 66-67 0.0033722974197709366 0.0 0.0 2.5935074843925863 0.0 68-69 0.0033722974197709366 0.0 0.0 2.911936668254457 0.0 70-71 0.00358306600850662 0.0 0.0 3.244487347561618 0.0 72-73 0.0037938345972423036 0.0 0.0 3.5819700118451947 0.0 74-75 0.003878142032736577 0.0 0.0 3.9132560796199423 0.0 76-77 0.003878142032736577 0.0 0.0 4.274639901866145 0.0 78-79 0.004004603185977987 0.0 4.215371774713671E-5 4.6770392914803125 0.0 80-81 0.004889831258667858 0.0 8.430743549427342E-5 5.101822305218208 0.0 82-83 0.004974138694162132 0.0 8.430743549427342E-5 5.537565285570361 0.0 84-85 0.005184907282897815 0.0 8.430743549427342E-5 5.969725199914007 0.0 86-87 0.005395675871633499 0.0 8.430743549427342E-5 6.414025384968827 0.0 88-89 0.005395675871633499 0.0 8.430743549427342E-5 6.894577767286186 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3730 0.0 49.731384 1 GTATCAA 6330 0.0 39.59877 1 ATCAACG 7970 0.0 31.32978 3 TATCAAC 7970 0.0 31.210428 2 TCAACGC 8080 0.0 30.903263 4 CAACGCA 8195 0.0 30.52764 5 AACGCAG 8440 0.0 29.669 6 ACGCAGA 9570 0.0 26.119421 7 CGCAGAG 9690 0.0 25.648836 8 GTACATG 7425 0.0 25.17508 1 TACATGG 7495 0.0 24.367783 2 GCAGAGT 10065 0.0 23.983986 9 CGTTCGC 60 1.715873E-4 23.741192 46-47 TAGCCCT 835 0.0 23.353598 4 GAGTACT 5430 0.0 22.839876 12-13 ACATGGG 7560 0.0 22.774202 3 GTAGGAC 1360 0.0 22.032207 3 TAGGACC 770 0.0 21.618906 4 GTACTTT 5685 0.0 21.560099 14-15 TGGTATC 1725 0.0 21.506113 2 >>END_MODULE